AR111790A1 - Método para la producción de una planta resistente a herbicidas mediante la introducción de un sistema de edición de bases de un gen relacionado con la resistencia a herbicidas - Google Patents

Método para la producción de una planta resistente a herbicidas mediante la introducción de un sistema de edición de bases de un gen relacionado con la resistencia a herbicidas

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Publication number
AR111790A1
AR111790A1 ARP180101256A ARP180101256A AR111790A1 AR 111790 A1 AR111790 A1 AR 111790A1 AR P180101256 A ARP180101256 A AR P180101256A AR P180101256 A ARP180101256 A AR P180101256A AR 111790 A1 AR111790 A1 AR 111790A1
Authority
AR
Argentina
Prior art keywords
plant
base
fusion protein
guide rna
herbicide
Prior art date
Application number
ARP180101256A
Other languages
English (en)
Original Assignee
Inst Genetics & Developmental Biology Cas
Univ China Agricultural
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Filing date
Publication date
Application filed by Inst Genetics & Developmental Biology Cas, Univ China Agricultural filed Critical Inst Genetics & Developmental Biology Cas
Publication of AR111790A1 publication Critical patent/AR111790A1/es

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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Abstract

La presente divulgación pertenece al campo de la ingeniería genética de las plantas. Específicamente, se refiere a un método para crear plantas resistentes a herbicidas mediante técnicas de edición de bases, y un método para detectar sitios de mutaciones de genes endógenos capaces de conferir resistencia a herbicidas en plantas. Además se refiere al uso de los sitios de mutaciones de genes endógenos identificados en la reproducción de cultivos. Reivindicación 1: Un método para la producción de una planta resistente a herbicidas, preferentemente, una planta resistente a herbicidas libre de transgenes, que comprende introducir en la planta un sistema para la edición de bases de un gen relacionado con la resistencia a herbicidas en el genoma de la planta, por lo cual el ARN guía dirige la proteína de fusión de edición de bases a una secuencia objetivo de un gen relacionado con la resistencia a herbicidas en la planta, de modo de lograr una o más sustituciones de nucleótidos en la secuencia objetivo, donde dicho sistema comprende por lo menos uno de los siguientes (i) a (v): i) una proteína de fusión de edición de bases, y un ARN guía; ii) una construcción de expresión que comprende una secuencia de nucleótidos que codifica una proteína de fusión de edición de bases, y un ARN guía; iii) una proteína de fusión de edición de bases, y una construcción de expresión que comprende una secuencia de nucleótidos que codifica un ARN guía; iv) una construcción de expresión que comprende una secuencia de nucleótidos que codifica una proteína de fusión de edición de bases, y una construcción de expresión que comprende una secuencia de nucleótidos que codifica un ARN guía; v) una construcción de expresión que comprende una secuencia de nucleótidos que codifica proteína de fusión de edición de bases y una secuencia de nucleótidos que codifica ARN guía; donde dicha proteína de fusión de edición de bases contiene un dominio de nucleasa CRISPR inactivada por nucleasa (tal como Cas9 inactivada por nucleasa) y un dominio de desaminasa, donde dicho ARN guía puede dirigir dicha proteína de fusión de edición de bases a una secuencia objetivo en el gen relacionado con la resistencia a herbicidas en el genoma de la planta, donde dichas una o más sustituciones de nucleótidos confieren resistencia a herbicidas a la planta, opcionalmente, donde dicho método además comprende una etapa de cribado de la planta para detectar la resistencia a herbicidas.
ARP180101256A 2017-05-11 2018-05-11 Método para la producción de una planta resistente a herbicidas mediante la introducción de un sistema de edición de bases de un gen relacionado con la resistencia a herbicidas AR111790A1 (es)

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Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019233349A1 (zh) * 2018-06-04 2019-12-12 青岛清原化合物有限公司 突变型对羟苯基丙酮酸双氧化酶、其编码核酸以及应用
CN111139261B (zh) * 2019-02-28 2022-05-31 山东省农业科学院作物研究所 一种利用基因编辑降低小麦籽粒多酚氧化酶含量的方法
CN110373414A (zh) * 2019-08-01 2019-10-25 中国农业科学院作物科学研究所 一种高效创制磺酰脲类除草剂抗性玉米的方法及其应用
US20230257761A1 (en) * 2019-09-12 2023-08-17 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Herbicide resistant plant
EP3889266A4 (en) * 2019-11-07 2022-08-10 Qingdao KingAgroot Chemical Compound Co., Ltd. METHOD FOR GENERATION OF NEW MUTATIONS IN ORGANISMS, AND ASSOCIATED APPLICATION
CN112813064A (zh) * 2019-11-18 2021-05-18 安徽省农业科学院水稻研究所 一种创制高抗稳定的内源抗除草剂水稻的方法
CN110982818B (zh) * 2019-12-20 2022-03-08 北京市农林科学院 核定位信号f4nls在高效创制水稻除草剂抗性材料中的应用
CN111423990B (zh) * 2020-04-10 2021-08-27 科稷达隆(北京)生物技术有限公司 一种乙氧氟草醚敏感型酵母菌及其制备方法
CN111560396B (zh) * 2020-05-07 2022-03-25 海南波莲水稻基因科技有限公司 植物转基因筛选载体pCALSm1及其应用
CN115786285A (zh) * 2020-09-23 2023-03-14 山东舜丰生物科技有限公司 抗除草剂乙酰辅酶a羧化酶突变体及其应用
CN116724119A (zh) * 2020-12-17 2023-09-08 苏州齐禾生科生物科技有限公司 除草剂抗性植物
WO2022151352A1 (zh) * 2021-01-15 2022-07-21 江苏省农业科学院 具有除草剂抗性的hppd突变型蛋白及其应用
CN112813094B (zh) * 2021-02-08 2023-03-24 山东师范大学 一种抗二硝基苯胺类除草剂小麦的构建方法
CN113265401B (zh) * 2021-04-15 2022-06-03 江苏里下河地区农业科学研究所 一种通过基因编辑提高水稻对HPPD抑制剂类除草剂抗性的方法及其专用sgRNA
CN113201557B (zh) * 2021-05-10 2023-04-07 安徽省农业科学院水稻研究所 一种引导编辑系统介导作物产生内源除草剂抗性的方法
KR20230139064A (ko) * 2022-03-25 2023-10-05 기초과학연구원 식물세포 소기관 dna의 아데닌을 구아닌으로 염기 교정

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6919619B2 (en) 2003-02-28 2005-07-19 The Regents Of The University Of Michigan Actively-shielded signal wires
WO2007149069A2 (en) * 2006-06-15 2007-12-27 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Resistance to acetolactate synthase-inhibiting herbicides
TW201113376A (en) 2009-09-01 2011-04-16 Basf Agrochemical Products Bv Herbicide-tolerant plants
US20130047297A1 (en) 2010-03-08 2013-02-21 Robert D. Sammons Polynucleotide molecules for gene regulation in plants
UA123491C2 (uk) * 2011-02-01 2021-04-14 Колорадо Уіт Рісерч Фаундейшн, Інк. РОСЛИНА, СТІЙКА ДО КВІЗАЛОФОП-р-ЕТИЛУ, ТА СПОСІБ ЇЇ ОДЕРЖАННЯ
US9540627B2 (en) * 2011-11-14 2017-01-10 Basf Se Methods and compositions for isolating, identifying and characterizing monocot plastidic accase herbicide tolerant mutations using a model system
CN105263312A (zh) * 2013-04-05 2016-01-20 美国陶氏益农公司 用于在植物基因组内整合外源序列的方法和组合物
WO2014186686A2 (en) * 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
AU2014310621A1 (en) * 2013-08-21 2016-02-18 Bayer Cropscience Lp ALS inhibitor herbicide tolerant mutant plants
EP3611268A1 (en) 2013-08-22 2020-02-19 E. I. du Pont de Nemours and Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
EP3613854A1 (en) * 2014-03-05 2020-02-26 National University Corporation Kobe University Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
HUE051354T2 (hu) * 2014-04-14 2021-03-01 Nemesis Bioscience Ltd Terapeutikum
BR112018002026A2 (pt) 2015-08-14 2018-09-18 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences método para se obter arroz resistente a glifosato através da substituição de nucleotídeo direcionada ao sítio
JP2018531024A (ja) * 2015-10-20 2018-10-25 パイオニア ハイ−ブレッド インターナショナル， イン マーカーフリーゲノム改変のための方法および組成物
IL310721A (en) 2015-10-23 2024-04-01 Harvard College Nucleobase editors and their uses
EP3538661A4 (en) * 2016-11-14 2020-04-15 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences BASIC EDITING METHOD IN PLANTS

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