AR103927A1 - Método para mejorar la habilidad de resistir a adn de virus que infectan plantas - Google Patents
Método para mejorar la habilidad de resistir a adn de virus que infectan plantasInfo
- Publication number
- AR103927A1 AR103927A1 ARP160100673A ARP160100673A AR103927A1 AR 103927 A1 AR103927 A1 AR 103927A1 AR P160100673 A ARP160100673 A AR P160100673A AR P160100673 A ARP160100673 A AR P160100673A AR 103927 A1 AR103927 A1 AR 103927A1
- Authority
- AR
- Argentina
- Prior art keywords
- dna
- virus
- sequence
- ability
- resist
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/10—Vectors comprising a non-peptidic targeting moiety
Abstract
La presente divulga un método para mejorar la habilidad de resistir contra un ADN de virus que infecta una planta. La presente provee un método para elaborar una planta con mejor habilidad para resistir contra un ADN de virus que específicamente puede comprender los siguientes pasos: 1) seleccionar una secuencia que cumple con 5-NX-NGG-3 o 5-CCN-NX-3 de la secuencia genómica del ADN de virus como la secuencia diana; designar una secuencia de ADN reversa-complementaria a dicha secuencia diana; 2) construir dicha secuencia de ADN para un vector para expresar la nucleasa Cas9 del sistema CRISPR de manera de obtener un vector recombinante capaz de transcribir un ARN guía y expresar una proteína Cas9; el ARNcr en dicho ARN guía contiene un fragmento de ARN transcripto desde dicho fragmento de ADN; 3) introducir dicho vector recombinante en una planta receptora de manera de obtener una planta con habilidad mejorada para resistir contra dicho ADN de virus. El método de la presente depende meramente de la secuencia genómica del virus, sin la necesidad de conocer las funciones específicas de los genes virales. Por lo tanto, el método puede utilizarse ampliamente para resistir contra diversos ADN de virus bicatenario conocidos o virus monocatenario con ADN bicatenario como intermedio.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510107492 | 2015-03-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR103927A1 true AR103927A1 (es) | 2017-06-14 |
Family
ID=56879162
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP160100673A AR103927A1 (es) | 2015-03-12 | 2016-03-14 | Método para mejorar la habilidad de resistir a adn de virus que infectan plantas |
Country Status (11)
Country | Link |
---|---|
US (1) | US20180195084A1 (es) |
EP (1) | EP3309255A4 (es) |
JP (1) | JP2018507705A (es) |
KR (1) | KR101994953B1 (es) |
CN (1) | CN105969792A (es) |
AR (1) | AR103927A1 (es) |
AU (1) | AU2016228599A1 (es) |
BR (1) | BR112017016423A2 (es) |
CA (1) | CA2979292A1 (es) |
EA (1) | EA201791991A1 (es) |
WO (1) | WO2016141893A1 (es) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
JP7109784B2 (ja) | 2015-10-23 | 2022-08-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子編集のための進化したCas9蛋白質 |
WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
CN106939317A (zh) * | 2017-03-24 | 2017-07-11 | 华南农业大学 | 一种提高植物抵御rna病毒的能力的方法 |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
CN109504704B (zh) * | 2018-12-07 | 2022-07-12 | 华南农业大学 | 一种增强单子叶植物抵御rna病毒侵染的方法 |
MX2021011426A (es) | 2019-03-19 | 2022-03-11 | Broad Inst Inc | Metodos y composiciones para editar secuencias de nucleótidos. |
IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
US20230183733A1 (en) * | 2020-05-20 | 2023-06-15 | KWS SAAT SE & Co. KGaA | Multiple virus resistance |
CN115595330A (zh) * | 2021-07-12 | 2023-01-13 | 中国科学院微生物研究所(Cn) | 一种CRISPR-Cas3系统及其在抗植物病毒方面的应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040261149A1 (en) * | 2003-02-24 | 2004-12-23 | Fauquet Claude M. | siRNA-mediated inhibition of gene expression in plant cells |
MX2015011985A (es) * | 2013-03-15 | 2016-04-07 | Univ Minnesota | Ingenieria genomica de plantas utilizando sistemas crispr/cas. |
CN115261411A (zh) * | 2013-04-04 | 2022-11-01 | 哈佛学院校长同事会 | 利用CRISPR/Cas系统的基因组编辑的治疗性用途 |
CN103343120B (zh) * | 2013-07-04 | 2015-03-04 | 中国科学院遗传与发育生物学研究所 | 一种小麦基因组定点改造方法 |
CN103397018B (zh) * | 2013-08-20 | 2015-04-22 | 中国医学科学院医学生物学研究所 | 一种dna病毒基因组的定点改造方法 |
US20160237451A1 (en) * | 2013-09-30 | 2016-08-18 | Regents Of The University Of Minnesota | Conferring resistance to geminiviruses in plants using crispr/cas systems |
CN103757053B (zh) * | 2014-01-28 | 2016-05-25 | 中国医学科学院医学生物学研究所 | 一种特异性的dna病毒基因组定点改造及筛选方法 |
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2016
- 2016-03-14 EP EP16761120.1A patent/EP3309255A4/en not_active Withdrawn
- 2016-03-14 JP JP2017548173A patent/JP2018507705A/ja active Pending
- 2016-03-14 WO PCT/CN2016/076246 patent/WO2016141893A1/zh active Application Filing
- 2016-03-14 US US15/557,306 patent/US20180195084A1/en not_active Abandoned
- 2016-03-14 EA EA201791991A patent/EA201791991A1/ru unknown
- 2016-03-14 CA CA2979292A patent/CA2979292A1/en not_active Abandoned
- 2016-03-14 AU AU2016228599A patent/AU2016228599A1/en not_active Abandoned
- 2016-03-14 AR ARP160100673A patent/AR103927A1/es unknown
- 2016-03-14 BR BR112017016423A patent/BR112017016423A2/pt not_active Application Discontinuation
- 2016-03-14 KR KR1020177029290A patent/KR101994953B1/ko active IP Right Grant
- 2016-03-14 CN CN201610141330.1A patent/CN105969792A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
EP3309255A4 (en) | 2018-08-01 |
BR112017016423A2 (pt) | 2018-04-10 |
WO2016141893A1 (zh) | 2016-09-15 |
AU2016228599A1 (en) | 2017-08-17 |
EP3309255A1 (en) | 2018-04-18 |
EA201791991A1 (ru) | 2018-07-31 |
JP2018507705A (ja) | 2018-03-22 |
KR101994953B1 (ko) | 2019-07-01 |
KR20170126495A (ko) | 2017-11-17 |
CN105969792A (zh) | 2016-09-28 |
US20180195084A1 (en) | 2018-07-12 |
CA2979292A1 (en) | 2016-09-15 |
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