MX2018001617A - Composiciones de crispr-cas9 diseñados y metodos de uso. - Google Patents

Composiciones de crispr-cas9 diseñados y metodos de uso.

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Publication number
MX2018001617A
MX2018001617A MX2018001617A MX2018001617A MX2018001617A MX 2018001617 A MX2018001617 A MX 2018001617A MX 2018001617 A MX2018001617 A MX 2018001617A MX 2018001617 A MX2018001617 A MX 2018001617A MX 2018001617 A MX2018001617 A MX 2018001617A
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MX
Mexico
Prior art keywords
systems
cas9
caspn
methods
nexus
Prior art date
Application number
MX2018001617A
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English (en)
Inventor
Paul May Andrew
Daniel Donohoue Paul
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Caribou Biosciences Inc
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Publication date
Application filed by Caribou Biosciences Inc filed Critical Caribou Biosciences Inc
Publication of MX2018001617A publication Critical patent/MX2018001617A/es

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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2310/53—Physical structure partially self-complementary or closed
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Abstract

La presente especificación describe sistemas de polinucleótidos de nexo de división asociados a CRISPR-Cas9 tipo II (sn-casPN), incluyendo sistemas que comprenden tres polinucleótidos de nexo de división (sn1-casPN/sn2-casPN/sn3-casPN) y sistemas que comprenden dos polinucleótidos de nexo de división (sn1-casPN/sn2-casPN). Junto con una proteína Cas9, sn-casPN facilitan modificaciones específicas de sitio, incluyendo corte y mutagénesis, de un polinucleótido objetivo in vitro e in vivo. Adicionalmente, los sistemas de polinucleótidos de nexo de división asociado a CRISR-Cas9 tipo II que comprenden sn-casPN son útiles en métodos para regular la expresión de un ácido nucleico objetivo. Se describen métodos en la presente para la creación de una variedad de sistemas de polinucleótidos de nexo de división asociado a CRISPR-Cas9 tipo II diseñados que comprenden dos o más sn-casPN. Las secuencias de polinucleótidos, casetes de expresión, vectores, composiciones y kits para realizar una variedad de métodos también se describen. Además, la presente especificación proporciona células genéticamente modificadas, composiciones de células modificadas, organismos transgénicos, composiciones farmacéuticas, así como una variedad de composiciones y métodos que involucran los sistemas de polinucleótidos de nexo de división asociados a CRISPR-cas9 tipo II diseñados.
MX2018001617A 2015-08-07 2016-08-05 Composiciones de crispr-cas9 diseñados y metodos de uso. MX2018001617A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562202715P 2015-08-07 2015-08-07
US201562209334P 2015-08-24 2015-08-24
PCT/US2016/045915 WO2017027423A1 (en) 2015-08-07 2016-08-05 Engineered crispr-cas9 compositions and methods of use

Publications (1)

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MX2018001617A true MX2018001617A (es) 2018-06-06

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MX2018001617A MX2018001617A (es) 2015-08-07 2016-08-05 Composiciones de crispr-cas9 diseñados y metodos de uso.

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US (5) US9580727B1 (es)
EP (2) EP3320092B1 (es)
JP (1) JP6649468B2 (es)
KR (1) KR102012382B1 (es)
CN (1) CN107922944B (es)
AU (1) AU2016304795B2 (es)
CA (1) CA2994166C (es)
DK (1) DK3320092T3 (es)
ES (1) ES2705540T3 (es)
GB (1) GB2556276A (es)
HK (1) HK1247238B (es)
IL (1) IL257307B (es)
MX (1) MX2018001617A (es)
NZ (1) NZ738689A (es)
WO (1) WO2017027423A1 (es)
ZA (1) ZA201800330B (es)

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