AR107260A1 - Una soja transgénica resistente al glifosato y el método de preparación de la misma - Google Patents

Una soja transgénica resistente al glifosato y el método de preparación de la misma

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Publication number
AR107260A1
AR107260A1 ARP160104073A ARP160104073A AR107260A1 AR 107260 A1 AR107260 A1 AR 107260A1 AR P160104073 A ARP160104073 A AR P160104073A AR P160104073 A ARP160104073 A AR P160104073A AR 107260 A1 AR107260 A1 AR 107260A1
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Argentina
Prior art keywords
glyphosate
transgenic
transgenic soybeans
dna
same preparation
Prior art date
Application number
ARP160104073A
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English (en)
Inventor
Hui Yang
Shuang Wang
Longguo Jin
Huilong Hong
Lijuan Zhang
Yong Guo
Bingfu Guo
Lijuan Qiu
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Inst Of Crop Science Chinese Academy Of Agriculture Sciences
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Application filed by Inst Of Crop Science Chinese Academy Of Agriculture Sciences filed Critical Inst Of Crop Science Chinese Academy Of Agriculture Sciences
Publication of AR107260A1 publication Critical patent/AR107260A1/es

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Medicinal Chemistry (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente describe una soja transgénica resistente al glifosato y su método de preparación y aplicación. Proporciona un método para el fitomejoramiento de la soja transgénica, que comprende: insertar el fragmento de ADN exógeno en las posiciones 50, 543, 767 - 50, 543, 792 del cromosoma 19 del genoma de soja blanco para obtener la soja transgénica. La soja transgénica divulgada tiene tolerancia a la dosis alta de glifosato, y también se puede mejorar mediante la hibridación con una cepa de soja excelente para optimizar otros caracteres agronómicos tales como rendimiento, calidad etc. El método de detección de la presente divulgación puede identificar la región de unión del ADN-T insertado y ADN del genoma de la planta y además identificar célula, tejido, órgano, producto, etc. de la soja transgénica y su descendiente.
ARP160104073A 2015-12-30 2016-12-29 Una soja transgénica resistente al glifosato y el método de preparación de la misma AR107260A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201511022465.8A CN105505981B (zh) 2015-12-30 2015-12-30 一种抗草甘膦转基因大豆及其制备方法与应用

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AR107260A1 true AR107260A1 (es) 2018-04-11

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CN (1) CN105505981B (es)
AR (1) AR107260A1 (es)
WO (1) WO2017113573A1 (es)

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CN105505981B (zh) * 2015-12-30 2018-12-25 中国农业科学院作物科学研究所 一种抗草甘膦转基因大豆及其制备方法与应用
CN106350532A (zh) * 2016-08-28 2017-01-25 浙江大学 一种抗草甘膦融合基因、编码蛋白及其应用
CN108179146B (zh) * 2018-02-03 2022-02-11 吉林省农业科学院 抗病转基因大豆事件b5c9120-3外源插入片段侧翼序列及其应用
CN108239639B (zh) * 2018-02-03 2022-02-11 吉林省农业科学院 耐逆转基因大豆事件wb1外源插入片段侧翼序列及其应用
CN108179147B (zh) * 2018-02-03 2022-02-11 吉林省农业科学院 高油酸转基因大豆事件e2d9050外源插入片段侧翼序列及其应用
CN112359123A (zh) * 2020-10-10 2021-02-12 浙江省农业科学院 转g10evo-epsps基因大豆实时荧光定量检测方法及其试剂盒
CN112322631B (zh) * 2020-11-06 2022-08-16 武汉天问生物科技有限公司 一种抗草甘膦转基因大豆的培育方法
CN114807221B (zh) * 2022-04-28 2023-06-06 宁波大学科学技术学院 水稻根系伸长调控基因OsDRP1C的突变体、蛋白质、表达载体及其应用

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EP1910547A2 (en) * 2005-07-29 2008-04-16 Monsanto Technology, LLC Development of novel germplasm using segregates from transgenic crosses
CN100365133C (zh) * 2005-10-21 2008-01-30 天津师范大学 一种转基因大豆的检测方法及所用引物
CN101100676B (zh) * 2007-06-15 2010-12-22 中国农业科学院生物技术研究所 培育抗草甘膦植物的双价表达载体
TW201231655A (en) * 2010-12-15 2012-08-01 Syngenta Participations Ag Soybean event SYHT04R and compositions and methods for detection thereof
CN104450755B (zh) * 2014-11-14 2017-07-11 重庆大学 一种东亚飞蝗ATP合酶α亚基基因及其dsRNA在害虫防治中的应用
CN105505981B (zh) * 2015-12-30 2018-12-25 中国农业科学院作物科学研究所 一种抗草甘膦转基因大豆及其制备方法与应用

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WO2017113573A1 (zh) 2017-07-06
CN105505981A (zh) 2016-04-20

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