AR120421A1 - Método para producir una mutación en un gen de un organismo y su uso - Google Patents

Método para producir una mutación en un gen de un organismo y su uso

Info

Publication number
AR120421A1
AR120421A1 ARP200103100A ARP200103100A AR120421A1 AR 120421 A1 AR120421 A1 AR 120421A1 AR P200103100 A ARP200103100 A AR P200103100A AR P200103100 A ARP200103100 A AR P200103100A AR 120421 A1 AR120421 A1 AR 120421A1
Authority
AR
Argentina
Prior art keywords
different
organism
gene
creating
protein domains
Prior art date
Application number
ARP200103100A
Other languages
English (en)
Inventor
Huarong Li
Bo Chen
Sudong Mo
Jiyao Wang
Linjian Jiang
Original Assignee
Qingdao Kingagroot Chemical Compounds Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Kingagroot Chemical Compounds Co Ltd filed Critical Qingdao Kingagroot Chemical Compounds Co Ltd
Publication of AR120421A1 publication Critical patent/AR120421A1/es

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente se refiere a los campos técnicos de ingeniería genética y bioinformática, en particular, a un método para crear un gen en un organismo en ausencia de un molde de ADN artificial y uno de sus usos. El método comprende generar simultáneamente rupturas de ADN en dos o más sitios específicos diferentes en el genoma del organismo, en donde los sitios específicos son sitios genómicos capaces de separar diferentes elementos genéticos o diferentes dominios de proteína, y las rupturas de ADN se ligan entre sí a través de la unión de extremos no homólogos (NHEJ) o reparación homóloga para generar una combinación de los diferentes elementos genéticos o diferentes dominios de proteína que es diferente de la secuencia del genoma original, de este modo se crean un gen. El gen de la presente puede cambiar el crecimiento, desarrollo, resistencia, rendimiento y otros rasgos del organismo, y tiene gran valor de aplicación.
ARP200103100A 2019-11-06 2020-11-09 Método para producir una mutación en un gen de un organismo y su uso AR120421A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911073406 2019-11-06

Publications (1)

Publication Number Publication Date
AR120421A1 true AR120421A1 (es) 2022-02-16

Family

ID=75750163

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP200103100A AR120421A1 (es) 2019-11-06 2020-11-09 Método para producir una mutación en un gen de un organismo y su uso

Country Status (16)

Country Link
US (1) US20220348950A1 (es)
EP (1) EP4074832A4 (es)
JP (1) JP2022553598A (es)
KR (1) KR20220066979A (es)
CN (1) CN112779266A (es)
AR (1) AR120421A1 (es)
AU (1) AU2020378500A1 (es)
BR (1) BR112022001455A2 (es)
CA (1) CA3159360A1 (es)
CL (1) CL2022001192A1 (es)
CO (1) CO2022007902A2 (es)
IL (1) IL292654A (es)
MX (1) MX2022005431A (es)
PE (1) PE20221178A1 (es)
WO (1) WO2021088923A1 (es)
ZA (1) ZA202206200B (es)

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KR20240005867A (ko) * 2021-05-02 2024-01-12 칭다오 킹아그루트 케미컬 컴파운드 컴퍼니 리미티드 유기체 내에서 새로운 유전자를 생성하는 방법 및 이의 용도
CN113652390B (zh) * 2021-09-22 2023-08-11 北京林业大学 一种紫薇原生质体的制备方法
CN114107367A (zh) * 2021-11-29 2022-03-01 广西壮族自治区林业科学研究院 一种靶向切除尾叶桉cad基因编码区dna的基因编辑方法
CN114634992B (zh) * 2022-03-25 2023-10-13 西南交通大学 一种暗紫贝母的Indel标记及其应用
CN114686498A (zh) * 2022-04-22 2022-07-01 中佳源物种(深圳)生物科技有限公司 苹果突变基因及编码蛋白、苹果突变体的制备方法
CN115644067B (zh) * 2022-12-09 2023-03-14 云南省农业科学院药用植物研究所 一种生产滇黄精双单倍体的方法
CN116426671B (zh) * 2023-03-03 2024-05-10 中国医学科学院药用植物研究所 基于时珍法的中药鉴定方法及应用
CN116904495A (zh) * 2023-05-26 2023-10-20 四川农业大学 白芷原生质体高效瞬时转化的方法

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KR20100133319A (ko) * 2009-06-11 2010-12-21 주식회사 툴젠 표적 특이적인 게놈의 재배열을 위한 표적 특이적 뉴클레아제 및 이의 용도
DK2816112T3 (en) 2009-12-10 2018-11-19 Univ Minnesota TAL effector-mediated DNA modification
MX2014014650A (es) 2012-05-30 2015-10-14 Univ Washington Minivectores superenrollados como una herramienta para la reparacion, alteración y reemplazo de ácido desoxirribonucleico.
CA3221516A1 (en) * 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
AU2015288157A1 (en) * 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
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CN109475099A (zh) * 2016-07-29 2019-03-15 孟山都技术公司 用于在植物中进行基因表达的方法和组合物
AU2018320864B2 (en) * 2017-08-22 2024-02-22 Napigen, Inc. Organelle genome modification using polynucleotide guided endonuclease
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US11932858B2 (en) * 2017-12-14 2024-03-19 Donald Danforth Plant Science Center Homologous recombination via transcriptional activation
WO2019196717A1 (zh) * 2018-04-13 2019-10-17 青岛清原化合物有限公司 一种随机突变的基因编辑系统及其应用

Also Published As

Publication number Publication date
CA3159360A1 (en) 2021-05-14
BR112022001455A2 (pt) 2022-05-24
ZA202206200B (en) 2023-11-29
EP4074832A1 (en) 2022-10-19
KR20220066979A (ko) 2022-05-24
PE20221178A1 (es) 2022-08-01
IL292654A (en) 2022-07-01
AU2020378500A1 (en) 2022-06-23
MX2022005431A (es) 2022-05-26
CO2022007902A2 (es) 2022-06-30
CL2022001192A1 (es) 2022-11-25
US20220348950A1 (en) 2022-11-03
EP4074832A4 (en) 2023-12-06
WO2021088923A1 (zh) 2021-05-14
CN112779266A (zh) 2021-05-11
JP2022553598A (ja) 2022-12-26

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