BR112022008916A2 - Método para gerar novas mutações em organismos e aplicação das mesmas - Google Patents
Método para gerar novas mutações em organismos e aplicação das mesmasInfo
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- BR112022008916A2 BR112022008916A2 BR112022008916A BR112022008916A BR112022008916A2 BR 112022008916 A2 BR112022008916 A2 BR 112022008916A2 BR 112022008916 A BR112022008916 A BR 112022008916A BR 112022008916 A BR112022008916 A BR 112022008916A BR 112022008916 A2 BR112022008916 A2 BR 112022008916A2
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- Prior art keywords
- new
- mutations
- dna
- repair
- sequence
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- C12N15/09—Recombinant DNA-technology
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- C12N15/8275—Glyphosate
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- C12N15/8277—Phosphinotricin
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Abstract
MÉTODO PARA GERAR NOVAS MUTAÇÕES EM ORGANISMOS E APLICAÇÃO DAS MESMAS. A presente invenção pertence ao campo técnico da engenharia genética, e refere-se especificamente a um método para gerar uma mutação específica de sítio em um organismo na ausência de um modelo de DNA artificial e um uso do mesmo. O método compreende as seguintes etapas: gerar sequencialmente duas ou mais quebras de DNA em um sítio específico em um genoma de um organismo e repará-las espontaneamente, respectivamente, em que uma quebra de DNA posterior é gerada com base em uma nova sequência gerada a partir de um reparo de quebra de DNA anterior. Na presente invenção, um novo alvo é projetado com base em uma sequência formada por um novo evento de reparo gerado por edição sequencial e, assim, as mutações podem ser sequencialmente formadas por várias vezes em um sítio específico no genoma, o que enriquece muito os tipos de eventos de reparo após quebras de DNA, e realiza novas mutações de substituição, exclusão e inserção de base que não podem ser obtidas em uma única edição de gene.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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CN201911081617 | 2019-11-07 | ||
CN202010821877 | 2020-08-15 | ||
CN202010974151 | 2020-09-16 | ||
PCT/CN2020/120633 WO2021088601A1 (zh) | 2019-11-07 | 2020-10-13 | 一种在生物体内产生新突变的方法及应用 |
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BR112022008916A2 true BR112022008916A2 (pt) | 2022-08-02 |
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Application Number | Title | Priority Date | Filing Date |
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BR112022008916A BR112022008916A2 (pt) | 2019-11-07 | 2020-10-13 | Método para gerar novas mutações em organismos e aplicação das mesmas |
Country Status (13)
Country | Link |
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US (1) | US20230287389A1 (pt) |
EP (1) | EP3889266A4 (pt) |
JP (1) | JP2023500357A (pt) |
KR (1) | KR20220093324A (pt) |
CN (1) | CN112251419B (pt) |
AU (1) | AU2020378387A1 (pt) |
BR (1) | BR112022008916A2 (pt) |
CA (1) | CA3159682A1 (pt) |
CL (1) | CL2021003593A1 (pt) |
IL (1) | IL289541A (pt) |
MX (1) | MX2022001245A (pt) |
WO (1) | WO2021088601A1 (pt) |
ZA (1) | ZA202203920B (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115161316A (zh) * | 2021-04-02 | 2022-10-11 | 上海科技大学 | 一种引导编辑工具、融合rna及其用途 |
CN113846075A (zh) * | 2021-11-29 | 2021-12-28 | 科稷达隆(北京)生物技术有限公司 | Mad7-nls融合蛋白、用于植物基因组定点编辑的核酸构建物及其应用 |
CN114145231B (zh) * | 2021-12-16 | 2022-09-13 | 广西壮族自治区中国科学院广西植物研究所 | 一种二色波罗蜜种苗快速繁殖方法 |
CN117987447A (zh) * | 2022-11-02 | 2024-05-07 | 广州大学 | 一种真核细胞持续进化的控制方法及其应用 |
CN115644067B (zh) * | 2022-12-09 | 2023-03-14 | 云南省农业科学院药用植物研究所 | 一种生产滇黄精双单倍体的方法 |
CN116286869B (zh) * | 2023-03-23 | 2024-04-05 | 石河子大学 | 一种羽毛针禾糖转运蛋白基因SpSWEET14在提高植物抗寒性中的应用 |
CN117918260B (zh) * | 2024-03-21 | 2024-05-24 | 西南林业大学 | 一种云南松组培苗的繁殖方法 |
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EA021541B9 (ru) * | 2007-04-04 | 2017-04-28 | Басф Плант Сайенс Гмбх | Рекомбинантный или мутантный полинуклеотид для придания растениям толерантности к гербицидам, ингибирующим ацетогидроксикислотную синтазу (ahas) (варианты), растение, не встречающееся в природе, и его семена, способ борьбы с сорняками, способ получения трансгенного растения, толерантного к соединениям, ингибирующим ahas, способ идентификации или отбора клетки растения, ткани растения или растения или части растения, способ предотвращения нежелательного вегетативного роста и способ идентификации растения |
WO2010039750A2 (en) * | 2008-10-01 | 2010-04-08 | Monsanto Technology Llc | Transgenic plants with enhanced agronomic traits |
EP3036331B1 (en) * | 2013-08-21 | 2021-06-16 | BASF Agricultural Solutions Seed US LLC | Als inhibitor herbicide tolerant mutant plants |
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WO2018140899A1 (en) * | 2017-01-28 | 2018-08-02 | Inari Agriculture, Inc. | Novel plant cells, plants, and seeds |
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AU2018263195B2 (en) * | 2017-05-05 | 2022-04-07 | Suzhou Qi Biodesign Biotechnology Company Limited | Methods for isolating cells without the use of transgenic marker sequences |
US11345924B2 (en) * | 2017-05-11 | 2022-05-31 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | Creation of herbicide resistant gene and use thereof |
CN110157727A (zh) * | 2017-12-21 | 2019-08-23 | 中国科学院遗传与发育生物学研究所 | 植物碱基编辑方法 |
KR20200119239A (ko) * | 2018-02-08 | 2020-10-19 | 지머젠 인코포레이티드 | 코리네박테리움에서 crispr을 사용하는 게놈 편집 |
WO2019196717A1 (zh) * | 2018-04-13 | 2019-10-17 | 青岛清原化合物有限公司 | 一种随机突变的基因编辑系统及其应用 |
US20190330643A1 (en) * | 2018-04-25 | 2019-10-31 | The Catholic University Of America | Engineering of bacteriophages by genome editing using the crispr-cas9 system |
CN109536527A (zh) * | 2018-11-27 | 2019-03-29 | 中国科学院动物研究所 | 一种基因点突变修复的新方法 |
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2020
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- 2020-10-13 AU AU2020378387A patent/AU2020378387A1/en active Pending
- 2020-10-13 CN CN202011089074.9A patent/CN112251419B/zh active Active
- 2020-10-13 MX MX2022001245A patent/MX2022001245A/es unknown
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WO2021088601A1 (zh) | 2021-05-14 |
EP3889266A4 (en) | 2022-08-10 |
IL289541A (en) | 2022-03-01 |
EP3889266A1 (en) | 2021-10-06 |
KR20220093324A (ko) | 2022-07-05 |
TW202130813A (zh) | 2021-08-16 |
US20230287389A1 (en) | 2023-09-14 |
CA3159682A1 (en) | 2021-05-14 |
CN112251419A (zh) | 2021-01-22 |
AU2020378387A1 (en) | 2022-01-20 |
ZA202203920B (en) | 2023-11-29 |
CL2021003593A1 (es) | 2022-08-19 |
JP2023500357A (ja) | 2023-01-05 |
CN112251419B (zh) | 2023-11-10 |
MX2022001245A (es) | 2022-02-22 |
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