BR112018076190A2 - ortólogos e sistemas crispr tipo vi - Google Patents
ortólogos e sistemas crispr tipo viInfo
- Publication number
- BR112018076190A2 BR112018076190A2 BR112018076190A BR112018076190A BR112018076190A2 BR 112018076190 A2 BR112018076190 A2 BR 112018076190A2 BR 112018076190 A BR112018076190 A BR 112018076190A BR 112018076190 A BR112018076190 A BR 112018076190A BR 112018076190 A2 BR112018076190 A2 BR 112018076190A2
- Authority
- BR
- Brazil
- Prior art keywords
- systems
- orthologs
- targeting
- crispr type
- rna
- Prior art date
Links
- 108091033409 CRISPR Proteins 0.000 title abstract 2
- 230000008685 targeting Effects 0.000 abstract 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 239000012636 effector Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Analytical Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Immunology (AREA)
- Compounds Of Unknown Constitution (AREA)
- Saccharide Compounds (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
a invenção fornece sistemas, métodos e composições para ácidos nucleicos de alvejamento. em particular, a invenção fornece sistemas de alvejamento de rna de ocorrência não natural ou geneticamente modificados que compreendem uma proteína efetora de crispr de alvejamento de rna inovadora e pelo menos um componente de ácido nucleico de alvejamento semelhante a um rna-guia.
Applications Claiming Priority (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662351662P | 2016-06-17 | 2016-06-17 | |
US201662351803P | 2016-06-17 | 2016-06-17 | |
US201662376377P | 2016-08-17 | 2016-08-17 | |
US201662410366P | 2016-10-19 | 2016-10-19 | |
US201662432240P | 2016-12-09 | 2016-12-09 | |
US201762471792P | 2017-03-15 | 2017-03-15 | |
US201762484786P | 2017-04-12 | 2017-04-12 | |
PCT/US2017/038154 WO2017219027A1 (en) | 2016-06-17 | 2017-06-19 | Type vi crispr orthologs and systems |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112018076190A2 true BR112018076190A2 (pt) | 2019-06-18 |
Family
ID=59295312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018076190A BR112018076190A2 (pt) | 2016-06-17 | 2017-06-19 | ortólogos e sistemas crispr tipo vi |
Country Status (9)
Country | Link |
---|---|
US (3) | US11788083B2 (pt) |
EP (1) | EP3455357A1 (pt) |
JP (3) | JP7267013B2 (pt) |
KR (2) | KR20190019168A (pt) |
CN (1) | CN109642231A (pt) |
AU (4) | AU2017283713B2 (pt) |
BR (1) | BR112018076190A2 (pt) |
CA (1) | CA3028158A1 (pt) |
WO (1) | WO2017219027A1 (pt) |
Families Citing this family (119)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
WO2016069124A1 (en) | 2014-09-15 | 2016-05-06 | Board Of Regents, The University Of Texas System | Improved single molecule peptide sequencing |
DE112012002570B4 (de) | 2011-06-23 | 2023-11-23 | Board Of Regents, The University Of Texas System | Identifizieren von Peptiden auf der Einzelmolekülebene |
CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
EP4079847A1 (en) | 2014-07-30 | 2022-10-26 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
EP3215617B1 (en) | 2014-11-07 | 2024-05-08 | Editas Medicine, Inc. | Systems for improving crispr/cas-mediated genome-editing |
EP3294896A1 (en) | 2015-05-11 | 2018-03-21 | Editas Medicine, Inc. | Optimized crispr/cas9 systems and methods for gene editing in stem cells |
CN116334142A (zh) | 2015-06-09 | 2023-06-27 | 爱迪塔斯医药公司 | 用于改善移植的crispr/cas相关方法和组合物 |
EP3786294A1 (en) | 2015-09-24 | 2021-03-03 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
IL258821B (en) | 2015-10-23 | 2022-07-01 | Harvard College | Nucleobase editors and their uses |
WO2017091630A1 (en) | 2015-11-23 | 2017-06-01 | The Regents Of The University Of California | Tracking and manipulating cellular rna via nuclear delivery of crispr/cas9 |
US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
EP3443086B1 (en) | 2016-04-13 | 2021-11-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
KR102547316B1 (ko) | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
EP3523426A4 (en) | 2016-09-30 | 2020-01-22 | The Regents of The University of California | RNA GUIDED NUCLEIC ACID MODIFYING ENZYMES AND METHOD FOR USE THEREOF |
EA201990860A1 (ru) | 2016-09-30 | 2019-10-31 | Рнк-направляемые модифицирующие нуклеиновые кислоты ферменты и способы их применения | |
GB2573062A (en) | 2016-10-14 | 2019-10-23 | Harvard College | AAV delivery of nucleobase editors |
PT3551753T (pt) | 2016-12-09 | 2022-09-02 | Harvard College | Diagnósticos baseados num sistema efetor de crispr |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
US11739308B2 (en) * | 2017-03-15 | 2023-08-29 | The Broad Institute, Inc. | Cas13b orthologues CRISPR enzymes and systems |
US11021740B2 (en) | 2017-03-15 | 2021-06-01 | The Broad Institute, Inc. | Devices for CRISPR effector system based diagnostics |
US11104937B2 (en) | 2017-03-15 | 2021-08-31 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
US11174515B2 (en) * | 2017-03-15 | 2021-11-16 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
KR102687373B1 (ko) | 2017-03-23 | 2024-07-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
WO2018183908A1 (en) | 2017-03-31 | 2018-10-04 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for treating ovarian tumors |
EP3610009A1 (en) | 2017-04-12 | 2020-02-19 | The Broad Institute, Inc. | Novel type vi crispr orthologs and systems |
US11618928B2 (en) | 2017-04-12 | 2023-04-04 | The Broad Institute, Inc. | CRISPR effector system based diagnostics for malaria detection |
CA3059956A1 (en) | 2017-04-21 | 2018-10-25 | The General Hospital Corporation | Variants of cpf1 (cas12a) with altered pam specificity |
EP3615672A1 (en) | 2017-04-28 | 2020-03-04 | Editas Medicine, Inc. | Methods and systems for analyzing guide rna molecules |
JP7398279B2 (ja) | 2017-05-10 | 2023-12-14 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Crispr/cas9核送達による細胞rnaの狙いを定めた編集 |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
EP3635104A1 (en) | 2017-06-09 | 2020-04-15 | Editas Medicine, Inc. | Engineered cas9 nucleases |
US11897953B2 (en) | 2017-06-14 | 2024-02-13 | The Broad Institute, Inc. | Compositions and methods targeting complement component 3 for inhibiting tumor growth |
US20200248169A1 (en) * | 2017-06-26 | 2020-08-06 | The Broad Institute, Inc. | Crispr/cas-cytidine deaminase based compositions, systems, and methods for targeted nucleic acid editing |
US11168322B2 (en) | 2017-06-30 | 2021-11-09 | Arbor Biotechnologies, Inc. | CRISPR RNA targeting enzymes and systems and uses thereof |
US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
US12049643B2 (en) | 2017-07-14 | 2024-07-30 | The Broad Institute, Inc. | Methods and compositions for modulating cytotoxic lymphocyte activity |
CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
JP2020537516A (ja) * | 2017-10-04 | 2020-12-24 | ザ・ブロード・インスティテュート・インコーポレイテッド | 標的化された核酸編集のためのシステム、方法、及び組成物 |
RU2020115264A (ru) | 2017-10-04 | 2021-11-08 | Зе Броад Институт, Инк. | Диагностика на основе эффекторной системы crispr |
US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
US20200332272A1 (en) * | 2017-10-23 | 2020-10-22 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
WO2019089808A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Class 2 crispr/cas compositions and methods of use |
AU2018358051A1 (en) | 2017-11-01 | 2020-05-14 | The Regents Of The University Of California | CasZ compositions and methods of use |
US11332736B2 (en) | 2017-12-07 | 2022-05-17 | The Broad Institute, Inc. | Methods and compositions for multiplexing single cell and single nuclei sequencing |
EP3728576A4 (en) * | 2017-12-22 | 2021-11-24 | The Broad Institute, Inc. | CAS12B SYSTEMS, METHODS AND COMPOSITIONS FOR SPECIFIC EDITING OF RNA BASES |
KR20200111180A (ko) * | 2017-12-22 | 2020-09-28 | 더 브로드 인스티튜트, 인코퍼레이티드 | Crispr 이펙터 시스템 기반 다중 진단 |
JP7527961B2 (ja) * | 2018-01-29 | 2024-08-05 | ザ・ブロード・インスティテュート・インコーポレイテッド | Crisprエフェクター系に基づく診断 |
CN108559738A (zh) * | 2018-02-12 | 2018-09-21 | 南昌大学 | 一种植物rna修饰和编辑的系统及方法 |
EP4257696A3 (en) | 2018-03-14 | 2024-01-24 | Arbor Biotechnologies, Inc. | Novel crispr dna targeting enzymes and systems |
FI3765616T3 (fi) | 2018-03-14 | 2023-08-29 | Arbor Biotechnologies Inc | Uusia CRISPR-DNA- ja RNA-kohdennusentsyymejä ja -järjestelmiä |
WO2019189827A1 (ja) * | 2018-03-30 | 2019-10-03 | 国立大学法人 岡山大学 | 核酸構築物、医薬組成物、抗がん剤、抗ウイルス剤及び抗菌剤 |
CN108796036B (zh) * | 2018-04-03 | 2021-11-19 | 交弘生物科技(上海)有限公司 | 基于原核Argonaute蛋白的核酸检测方法及其应用 |
US11795456B2 (en) | 2018-04-16 | 2023-10-24 | Georgia Tech Research Corporation | MRNA driven expression of RNA editors for treatment of pathologies |
CN112513250A (zh) * | 2018-04-20 | 2021-03-16 | 加利福尼亚大学董事会 | 用于跟踪和操纵细胞rna的融合蛋白和融合核糖核酸 |
US11957695B2 (en) | 2018-04-26 | 2024-04-16 | The Broad Institute, Inc. | Methods and compositions targeting glucocorticoid signaling for modulating immune responses |
CN108546718B (zh) * | 2018-05-16 | 2021-07-09 | 康春生 | crRNA介导的CRISPR/Cas13a基因编辑系统在肿瘤细胞中的应用 |
US20210198664A1 (en) | 2018-05-16 | 2021-07-01 | Arbor Biotechnologies, Inc. | Novel crispr-associated systems and components |
CN110527697B (zh) * | 2018-05-23 | 2023-07-07 | 中国科学院分子植物科学卓越创新中心 | 基于CRISPR-Cas13a的RNA定点编辑技术 |
US12036240B2 (en) | 2018-06-14 | 2024-07-16 | The Broad Institute, Inc. | Compositions and methods targeting complement component 3 for inhibiting tumor growth |
US20190390193A1 (en) * | 2018-06-23 | 2019-12-26 | John Lawrence Mee | Reversible method for sustainable human cognitive enhancement |
CA3102211A1 (en) | 2018-06-26 | 2020-01-02 | The Broad Institute, Inc. | Crispr double nickase based amplification compositions, systems, and methods |
US20210269866A1 (en) | 2018-06-26 | 2021-09-02 | The Broad Institute, Inc. | Crispr effector system based amplification methods, systems, and diagnostics |
WO2020000454A1 (zh) * | 2018-06-29 | 2020-01-02 | 深圳市博奥康生物科技有限公司 | 一种表达LwCas13a基因的质粒、构建方法及其应用 |
CN112469832A (zh) * | 2018-07-23 | 2021-03-09 | 德克萨斯大学系统董事会 | 蛋白质上的翻译后修饰的单分子测序鉴别 |
WO2020022461A1 (ja) * | 2018-07-25 | 2020-01-30 | 国立研究開発法人産業技術総合研究所 | 植物における標的dnaのメチル化を抑制する方法 |
SG11202102068TA (en) * | 2018-07-31 | 2021-03-30 | Broad Inst Inc | Novel crispr enzymes and systems |
EP3830301B1 (en) | 2018-08-01 | 2024-05-22 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
EP3847255A4 (en) * | 2018-09-06 | 2024-01-10 | The Regents of The University of California | ADAR RECRUITMENT DEVELOPED RNA AND DNA BASE EDITING |
EP3650553B1 (en) * | 2018-11-07 | 2023-07-12 | Siemens Healthcare GmbH | Method for detection of specific nucleic acids |
MX2021005702A (es) | 2018-11-14 | 2021-09-21 | Massachusetts Inst Technology | Multiplexión de variantes virales altamente evolutivas con sherlock. |
MX2021005701A (es) | 2018-11-14 | 2021-09-23 | Broad Inst Inc | Sistema crispr a base de sistemas y métodos de diagnóstico de gotas. |
EP3882345A4 (en) * | 2018-11-15 | 2023-02-22 | China Agricultural University | CRISPR-CAS12J ENZYME AND SYSTEM |
WO2020102659A1 (en) * | 2018-11-15 | 2020-05-22 | The Broad Institute, Inc. | G-to-t base editors and uses thereof |
WO2020124050A1 (en) | 2018-12-13 | 2020-06-18 | The Broad Institute, Inc. | Tiled assays using crispr-cas based detection |
EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
US11739156B2 (en) | 2019-01-06 | 2023-08-29 | The Broad Institute, Inc. Massachusetts Institute of Technology | Methods and compositions for overcoming immunosuppression |
WO2020186231A2 (en) | 2019-03-14 | 2020-09-17 | The Broad Institute, Inc. | Crispr effector system based multiplex diagnostics |
WO2020191102A1 (en) | 2019-03-18 | 2020-09-24 | The Broad Institute, Inc. | Type vii crispr proteins and systems |
JP2022526908A (ja) | 2019-03-19 | 2022-05-27 | ザ ブロード インスティテュート,インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
AU2020248911B2 (en) * | 2019-03-26 | 2022-12-15 | Edigene Inc. | Method for identifying functional elements |
CN109913502A (zh) * | 2019-04-16 | 2019-06-21 | 和元生物技术(上海)股份有限公司 | 一种Cas9-gRNA表达系统及其应用 |
WO2020219742A1 (en) | 2019-04-24 | 2020-10-29 | Novartis Ag | Compositions and methods for selective protein degradation |
US20220403356A1 (en) * | 2019-07-12 | 2022-12-22 | Duke University | 3' utr crispr-dcas 13 engineering system and methods of using same |
CN110819652A (zh) * | 2019-08-21 | 2020-02-21 | 山东金城生物药业有限公司 | 一种谷氨酸棒杆菌同时基因敲除及基因表达抑制的双功能系统及应用 |
WO2021046257A1 (en) | 2019-09-03 | 2021-03-11 | The Broad Institute, Inc. | Crispr effector system based multiplex cancer diagnostics |
JP2022546592A (ja) | 2019-09-03 | 2022-11-04 | マイエロイド・セラピューティクス,インコーポレーテッド | ゲノム組込みのための方法および組成物 |
CA3151563A1 (en) * | 2019-09-20 | 2021-03-25 | Feng Zhang | Novel type vi crispr enzymes and systems |
US11844800B2 (en) | 2019-10-30 | 2023-12-19 | Massachusetts Institute Of Technology | Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia |
EP4069842A1 (en) | 2019-12-02 | 2022-10-12 | Shape Therapeutics Inc. | Therapeutic editing |
EP4100424A2 (en) * | 2020-02-07 | 2022-12-14 | University of Rochester | Targeted translation of rna with crispr-cas13 to enhance protein synthesis |
CN111337666B (zh) * | 2020-02-12 | 2021-04-02 | 山东大学 | I-motif重组介导的FRET探针及其原位成像癌细胞表面蛋白质同源二聚化的应用 |
WO2021168799A1 (en) * | 2020-02-28 | 2021-09-02 | Huigene Therapeutics Co., Ltd. | Type vi-e and type vi-f crispr-cas system and uses thereof |
US11851702B2 (en) | 2020-03-23 | 2023-12-26 | The Broad Institute, Inc. | Rapid diagnostics |
AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
JP7419168B2 (ja) * | 2020-06-10 | 2024-01-22 | 株式会社東芝 | 改変型piggyBacトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法 |
EP4222253A1 (en) * | 2020-09-30 | 2023-08-09 | HuidaGene Therapeutics Co., Ltd. | Engineered crispr/cas13 system and uses thereof |
WO2022068912A1 (en) | 2020-09-30 | 2022-04-07 | Huigene Therapeutics Co., Ltd. | Engineered crispr/cas13 system and uses thereof |
WO2022188039A1 (en) | 2021-03-09 | 2022-09-15 | Huigene Therapeutics Co., Ltd. | Engineered crispr/cas13 system and uses thereof |
CN112430586B (zh) * | 2020-11-16 | 2021-09-07 | 珠海舒桐医疗科技有限公司 | 一种VI-B型CRISPR/Cas13基因编辑系统及其应用 |
CN116917478A (zh) * | 2020-12-22 | 2023-10-20 | 亥姆霍兹慕尼黑中心-德国健康与环境研究中心(有限公司) | Crispr/cas13在rna病毒和/或细菌诱导的疾病的治疗中的应用 |
WO2022149568A1 (ja) * | 2021-01-05 | 2022-07-14 | 学校法人 川崎学園 | ヒトを含めた生物の細胞にある転写産物および、遺伝子導入されたrnaとその複合体精製ツール |
US20240102007A1 (en) | 2021-06-01 | 2024-03-28 | Arbor Biotechnologies, Inc. | Gene editing systems comprising a crispr nuclease and uses thereof |
WO2023282710A1 (ko) * | 2021-07-08 | 2023-01-12 | 한국과학기술원 | Cas13 단백질의 활성 조절을 통한 rna 발현 조절 또는 편집 방법 |
WO2023004391A2 (en) | 2021-07-21 | 2023-01-26 | Montana State University | Nucleic acid detection using type iii crispr complex |
EP4215612A1 (en) * | 2022-01-20 | 2023-07-26 | Sartorius Stedim Cellca GmbH | Genome editing with crispr/cas nucleases comprising collateral activity |
WO2024173645A1 (en) | 2023-02-15 | 2024-08-22 | Arbor Biotechnologies, Inc. | Gene editing method for inhibiting aberrant splicing in stathmin 2 (stmn2) transcript |
Family Cites Families (131)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4217344A (en) | 1976-06-23 | 1980-08-12 | L'oreal | Compositions containing aqueous dispersions of lipid spheres |
US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
US4186183A (en) | 1978-03-29 | 1980-01-29 | The United States Of America As Represented By The Secretary Of The Army | Liposome carriers in chemotherapy of leishmaniasis |
US4261975A (en) | 1979-09-19 | 1981-04-14 | Merck & Co., Inc. | Viral liposome particle |
US4485054A (en) | 1982-10-04 | 1984-11-27 | Lipoderm Pharmaceuticals Limited | Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV) |
US4501728A (en) | 1983-01-06 | 1985-02-26 | Technology Unlimited, Inc. | Masking of liposomes from RES recognition |
US5049386A (en) | 1985-01-07 | 1991-09-17 | Syntex (U.S.A.) Inc. | N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4946787A (en) | 1985-01-07 | 1990-08-07 | Syntex (U.S.A.) Inc. | N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4897355A (en) | 1985-01-07 | 1990-01-30 | Syntex (U.S.A.) Inc. | N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
US4797368A (en) | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
US4774085A (en) | 1985-07-09 | 1988-09-27 | 501 Board of Regents, Univ. of Texas | Pharmaceutical administration systems containing a mixture of immunomodulators |
DE122007000007I1 (de) | 1986-04-09 | 2007-05-16 | Genzyme Corp | Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern |
US4837028A (en) | 1986-12-24 | 1989-06-06 | Liposome Technology, Inc. | Liposomes with enhanced circulation time |
US4873316A (en) | 1987-06-23 | 1989-10-10 | Biogen, Inc. | Isolation of exogenous recombinant proteins from the milk of transgenic mammals |
US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5264618A (en) | 1990-04-19 | 1993-11-23 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
WO1991017424A1 (en) | 1990-05-03 | 1991-11-14 | Vical, Inc. | Intracellular delivery of biologically active substances by means of self-assembling lipid complexes |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5173414A (en) | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
GB9114259D0 (en) | 1991-07-02 | 1991-08-21 | Ici Plc | Plant derived enzyme and dna sequences |
US5587308A (en) | 1992-06-02 | 1996-12-24 | The United States Of America As Represented By The Department Of Health & Human Services | Modified adeno-associated virus vector capable of expression from a novel promoter |
BR9306802A (pt) | 1992-07-27 | 1998-12-08 | Pioneer Hi Bred Int | Processo independente de genótipos para produção de planta de soja transgénica e processo de regeneração de plantas de soja a partir de nodos cotiledonais |
US5593972A (en) | 1993-01-26 | 1997-01-14 | The Wistar Institute | Genetic immunization |
WO1994026877A1 (en) | 1993-05-17 | 1994-11-24 | The Regents Of The University Of California | Ribozyme gene therapy for hiv infection and aids |
US5814618A (en) | 1993-06-14 | 1998-09-29 | Basf Aktiengesellschaft | Methods for regulating gene expression |
US5789156A (en) | 1993-06-14 | 1998-08-04 | Basf Ag | Tetracycline-regulated transcriptional inhibitors |
US5543158A (en) | 1993-07-23 | 1996-08-06 | Massachusetts Institute Of Technology | Biodegradable injectable nanoparticles |
US6007845A (en) | 1994-07-22 | 1999-12-28 | Massachusetts Institute Of Technology | Nanoparticles and microparticles of non-linear hydrophilic-hydrophobic multiblock copolymers |
AU698739B2 (en) | 1995-06-06 | 1998-11-05 | Isis Pharmaceuticals, Inc. | Oligonucleotides having phosphorothioate linkages of high chiral purity |
US5985662A (en) | 1995-07-13 | 1999-11-16 | Isis Pharmaceuticals Inc. | Antisense inhibition of hepatitis B virus replication |
US5985309A (en) | 1996-05-24 | 1999-11-16 | Massachusetts Institute Of Technology | Preparation of particles for inhalation |
US5855913A (en) | 1997-01-16 | 1999-01-05 | Massachusetts Instite Of Technology | Particles incorporating surfactants for pulmonary drug delivery |
US5846946A (en) | 1996-06-14 | 1998-12-08 | Pasteur Merieux Serums Et Vaccins | Compositions and methods for administering Borrelia DNA |
GB9907461D0 (en) | 1999-03-31 | 1999-05-26 | King S College London | Neurite regeneration |
GB9720465D0 (en) | 1997-09-25 | 1997-11-26 | Oxford Biomedica Ltd | Dual-virus vectors |
JP4303418B2 (ja) | 1997-10-24 | 2009-07-29 | ライフ テクノロジーズ コーポレーション | 組換え部位を有する核酸を使用する組換えクローニング |
US6750059B1 (en) | 1998-07-16 | 2004-06-15 | Whatman, Inc. | Archiving of vectors |
US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US7868149B2 (en) | 1999-07-20 | 2011-01-11 | Monsanto Technology Llc | Plant genome sequence and uses thereof |
US6603061B1 (en) | 1999-07-29 | 2003-08-05 | Monsanto Company | Agrobacterium-mediated plant transformation method |
GB0024550D0 (pt) | 2000-10-06 | 2000-11-22 | Oxford Biomedica Ltd | |
US7776321B2 (en) | 2001-09-26 | 2010-08-17 | Mayo Foundation For Medical Education And Research | Mutable vaccines |
GB0125216D0 (en) | 2001-10-19 | 2001-12-12 | Univ Strathclyde | Dendrimers for use in targeted delivery |
US20090100536A1 (en) | 2001-12-04 | 2009-04-16 | Monsanto Company | Transgenic plants with enhanced agronomic traits |
JP2005512598A (ja) | 2001-12-21 | 2005-05-12 | オックスフォード バイオメディカ (ユーケー) リミテッド | Eiavなどのレンチウイルス発現ベクターを使用するトランスジェニック生物の作製方法 |
EP1519714B1 (en) | 2002-06-28 | 2010-10-20 | Protiva Biotherapeutics Inc. | Method and apparatus for producing liposomes |
GB0220467D0 (en) | 2002-09-03 | 2002-10-09 | Oxford Biomedica Ltd | Composition |
EP1558724A4 (en) | 2002-11-01 | 2006-08-02 | New England Biolabs Inc | ORGANIC RNA SCREENING AND ITS USE IN INTERRUPTING GENE TRANSMISSION IN THE ENVIRONMENT |
NZ544637A (en) | 2003-07-16 | 2010-04-30 | Protiva Biotherapeutics Inc | Lipid encapsulated interfering RNA |
NZ581166A (en) | 2003-09-15 | 2011-06-30 | Protiva Biotherapeutics Inc | Polyethyleneglycol-modified lipid compounds and uses thereof |
GB0325379D0 (en) | 2003-10-30 | 2003-12-03 | Oxford Biomedica Ltd | Vectors |
JP4764426B2 (ja) | 2004-06-07 | 2011-09-07 | プロチバ バイオセラピューティクス インコーポレイティッド | カチオン性脂質および使用方法 |
AU2005252273B2 (en) | 2004-06-07 | 2011-04-28 | Arbutus Biopharma Corporation | Lipid encapsulated interfering RNA |
EP2397490B1 (en) | 2004-07-16 | 2013-09-04 | THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Vaccine constructs and combinations of vaccines designed to improve the breadth of the immune response to diverse strains and clades of HIV |
GB0422877D0 (en) | 2004-10-14 | 2004-11-17 | Univ Glasgow | Bioactive polymers |
WO2007048046A2 (en) | 2005-10-20 | 2007-04-26 | Protiva Biotherapeutics, Inc. | Sirna silencing of filovirus gene expression |
WO2007049731A1 (ja) | 2005-10-28 | 2007-05-03 | Mitsubishi Tanabe Pharma Corporation | 新規細胞膜透過ペプチド |
WO2007051303A1 (en) | 2005-11-02 | 2007-05-10 | Protiva Biotherapeutics, Inc. | MODIFIED siRNA MOLECULES AND USES THEREOF |
GB0526211D0 (en) | 2005-12-22 | 2006-02-01 | Oxford Biomedica Ltd | Viral vectors |
US7915399B2 (en) | 2006-06-09 | 2011-03-29 | Protiva Biotherapeutics, Inc. | Modified siRNA molecules and uses thereof |
JP2008078613A (ja) | 2006-08-24 | 2008-04-03 | Rohm Co Ltd | 窒化物半導体の製造方法及び窒化物半導体素子 |
WO2008042156A1 (en) | 2006-09-28 | 2008-04-10 | Northwestern University | Maximizing oligonucleotide loading on gold nanoparticle |
CA2670096A1 (en) | 2006-11-21 | 2008-05-29 | The Samuel Roberts Noble Foundation, Inc. | Biofuel production methods and compositions |
WO2008149176A1 (en) | 2007-06-06 | 2008-12-11 | Cellectis | Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof |
CA2711179A1 (en) | 2007-12-31 | 2009-07-16 | Nanocor Therapeutics, Inc. | Rna interference for the treatment of heart failure |
AU2009238175C1 (en) | 2008-04-15 | 2023-11-30 | Arbutus Biopharma Corporation | Novel lipid formulations for nucleic acid delivery |
JP2011523557A (ja) | 2008-06-04 | 2011-08-18 | メディカル リサーチ カウンシル | ペプチド |
JP2011526619A (ja) | 2008-06-30 | 2011-10-13 | サイレンシード リミテッド | 局所ドラッグデリバリーシステム、その方法、および、その組成物 |
EP2309980A1 (en) | 2008-07-08 | 2011-04-20 | S.I.F.I. Societa' Industria Farmaceutica Italiana | Ophthalmic compositions for treating pathologies of the posterior segment of the eye |
AU2009311667B2 (en) | 2008-11-07 | 2016-04-14 | Massachusetts Institute Of Technology | Aminoalcohol lipidoids and uses thereof |
GB2465749B (en) | 2008-11-25 | 2013-05-08 | Algentech Sas | Plant cell transformation method |
US20120047601A1 (en) | 2009-02-17 | 2012-02-23 | The Regents Of The University Of California | Method of Reducing Acetylation in Plants to Improve Biofuel Production |
ES2613498T3 (es) | 2009-07-01 | 2017-05-24 | Protiva Biotherapeutics Inc. | Nuevas formulaciones de lípidos para el suministro de agentes terapéuticos a tumores sólidos |
US8236943B2 (en) | 2009-07-01 | 2012-08-07 | Protiva Biotherapeutics, Inc. | Compositions and methods for silencing apolipoprotein B |
US20110027239A1 (en) | 2009-07-29 | 2011-02-03 | Tissue Genesis, Inc. | Adipose-derived stromal cells (asc) as delivery tool for treatment of cancer |
US8927807B2 (en) | 2009-09-03 | 2015-01-06 | The Regents Of The University Of California | Nitrate-responsive promoter |
US8889394B2 (en) | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
US20120003201A1 (en) | 2010-04-21 | 2012-01-05 | Nicholas Susanne B | Vault agents for chronic kidney disease |
JP5952263B2 (ja) | 2010-04-26 | 2016-07-13 | サンガモ バイオサイエンシーズ, インコーポレイテッド | ジンクフィンガーヌクレアーゼを使ったrosa遺伝子座のゲノム編集 |
US8372951B2 (en) | 2010-05-14 | 2013-02-12 | National Tsing Hua University | Cell penetrating peptides for intracellular delivery |
EP2575894B1 (en) | 2010-05-28 | 2015-02-25 | Oxford Biomedica (UK) Ltd | Delivery of lentiviral vectors to the brain |
EP2609135A4 (en) | 2010-08-26 | 2015-05-20 | Massachusetts Inst Technology | POLY (BETA-AMINO ALCOHOLS), THEIR PREPARATION AND USES THEREOF |
US9405700B2 (en) | 2010-11-04 | 2016-08-02 | Sonics, Inc. | Methods and apparatus for virtualization in an integrated circuit |
CA2831392C (en) | 2011-03-28 | 2020-04-28 | Massachusetts Institute Of Technology | Conjugated lipomers and uses thereof |
AU2012236099A1 (en) | 2011-03-31 | 2013-10-03 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
US20120295960A1 (en) | 2011-05-20 | 2012-11-22 | Oxford Biomedica (Uk) Ltd. | Treatment regimen for parkinson's disease |
WO2013033436A1 (en) | 2011-09-01 | 2013-03-07 | University Of Iowa Research Foundation | Oligonucleotide-based probes for detection of bacterial nucleases |
PT2791160T (pt) | 2011-12-16 | 2022-07-04 | Modernatx Inc | Composições arnm modificado |
US20130185823A1 (en) | 2012-01-16 | 2013-07-18 | Academia Sinica | Mesoporous silica nanoparticle-mediated delivery of dna into arabidopsis root |
LT3241902T (lt) * | 2012-05-25 | 2018-06-25 | The Regents Of The University Of California | Būdai ir kompozicijos, skirti rnr nukreiptos tikslinės dnr modifikacijai ir rnr nukreiptos transkripcijos moduliacijai |
US8614194B1 (en) | 2012-07-25 | 2013-12-24 | Kaohsiung Medical University | Anionic cell penetrating peptide and its use for intracellular delivery |
CN116622704A (zh) | 2012-07-25 | 2023-08-22 | 布罗德研究所有限公司 | 可诱导的dna结合蛋白和基因组干扰工具及其应用 |
CN110592089B (zh) | 2012-10-23 | 2022-11-01 | 基因工具股份有限公司 | 用于切割靶dna的组合物及其用途 |
EP3434776A1 (en) | 2012-12-12 | 2019-01-30 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
DK2771468T3 (en) | 2012-12-12 | 2015-05-26 | Broad Inst Inc | Production systems, processes and optimized drive configurations for sequence manipulation |
KR20150105956A (ko) | 2012-12-12 | 2015-09-18 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작 및 치료적 적용을 위한 시스템, 방법 및 조성물의 전달, 유전자 조작 및 최적화 |
WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
PL2931898T3 (pl) | 2012-12-12 | 2016-09-30 | Le Cong | Projektowanie i optymalizacja systemów, sposoby i kompozycje do manipulacji sekwencją z domenami funkcjonalnymi |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
PL2784162T3 (pl) | 2012-12-12 | 2016-01-29 | Broad Inst Inc | Opracowanie systemów, metod oraz zoptymalizowanych kompozycji przewodnikowych do manipulacji sekwencyjnej |
WO2014093595A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
DK2898075T3 (en) | 2012-12-12 | 2016-06-27 | Broad Inst Inc | CONSTRUCTION AND OPTIMIZATION OF IMPROVED SYSTEMS, PROCEDURES AND ENZYME COMPOSITIONS FOR SEQUENCE MANIPULATION |
WO2014093701A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
US11332719B2 (en) | 2013-03-15 | 2022-05-17 | The Broad Institute, Inc. | Recombinant virus and preparations thereof |
EP3725885A1 (en) | 2013-06-17 | 2020-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
JP6702858B2 (ja) | 2013-06-17 | 2020-06-03 | ザ・ブロード・インスティテュート・インコーポレイテッド | ウイルス成分を使用して障害および疾患をターゲティングするためのCRISPR−Cas系および組成物の送達、使用および治療上の適用 |
EP3011035B1 (en) | 2013-06-17 | 2020-05-13 | The Broad Institute, Inc. | Assay for quantitative evaluation of target site cleavage by one or more crispr-cas guide sequences |
AU2014281026B2 (en) | 2013-06-17 | 2020-05-28 | Massachusetts Institute Of Technology | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
DK3011031T3 (da) | 2013-06-17 | 2020-12-21 | Broad Inst Inc | Fremføring og anvendelse af crispr-cas-systemerne, vektorer og sammensætninger til levermålretning og -terapi |
EP3011032B1 (en) | 2013-06-17 | 2019-10-16 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells |
KR20160034901A (ko) | 2013-06-17 | 2016-03-30 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작에 최적화된 crispr-cas 이중 닉카아제 시스템, 방법 및 조성물 |
WO2015065964A1 (en) | 2013-10-28 | 2015-05-07 | The Broad Institute Inc. | Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof |
US9068179B1 (en) * | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
EP3079726B1 (en) | 2013-12-12 | 2018-12-05 | The Broad Institute, Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components |
US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
CA3012631A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
CN109153980B (zh) * | 2015-10-22 | 2023-04-14 | 布罗德研究所有限公司 | Vi-b型crispr酶和系统 |
EP3390624A4 (en) * | 2015-12-18 | 2019-07-10 | The Regents of The University of California | MODIFIED POLYPEPTIDES AND METHOD OF USE THEREOF |
MX2018008416A (es) * | 2016-01-08 | 2019-11-11 | Univ California | Polipéptidos heterodímericos condicionalmente activos y métodos para su uso. |
US11427837B2 (en) * | 2016-01-12 | 2022-08-30 | The Regents Of The University Of California | Compositions and methods for enhanced genome editing |
WO2017136335A1 (en) * | 2016-02-01 | 2017-08-10 | The Regents Of The University Of California | Self-inactivating endonuclease-encoding nucleic acids and methods of using the same |
JP6998313B2 (ja) * | 2016-02-11 | 2022-02-04 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 細胞のゲノムにおける変異ジストロフィン遺伝子を修飾する方法及び組成物 |
WO2017160752A1 (en) * | 2016-03-14 | 2017-09-21 | Intellia Therapeutics, Inc. | Methods and compositions for gene editing |
TWI773666B (zh) * | 2016-03-30 | 2022-08-11 | 美商英特利亞醫療公司 | Crispr/cas 組分之脂質奈米粒子調配物 |
US20190093128A1 (en) * | 2016-03-31 | 2019-03-28 | The Regents Of The University Of California | Methods for genome editing in zygotes |
WO2017189336A1 (en) * | 2016-04-25 | 2017-11-02 | The Regents Of The University Of California | Methods and compositions for genomic editing |
US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
PT3551753T (pt) * | 2016-12-09 | 2022-09-02 | Harvard College | Diagnósticos baseados num sistema efetor de crispr |
US11021740B2 (en) * | 2017-03-15 | 2021-06-01 | The Broad Institute, Inc. | Devices for CRISPR effector system based diagnostics |
US11174515B2 (en) * | 2017-03-15 | 2021-11-16 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
-
2017
- 2017-06-19 KR KR1020197001527A patent/KR20190019168A/ko not_active IP Right Cessation
- 2017-06-19 CN CN201780050351.5A patent/CN109642231A/zh active Pending
- 2017-06-19 WO PCT/US2017/038154 patent/WO2017219027A1/en unknown
- 2017-06-19 KR KR1020237037115A patent/KR20230156150A/ko not_active Application Discontinuation
- 2017-06-19 US US16/310,577 patent/US11788083B2/en active Active
- 2017-06-19 EP EP17736810.7A patent/EP3455357A1/en active Pending
- 2017-06-19 BR BR112018076190A patent/BR112018076190A2/pt not_active Application Discontinuation
- 2017-06-19 JP JP2018566199A patent/JP7267013B2/ja active Active
- 2017-06-19 AU AU2017283713A patent/AU2017283713B2/en active Active
- 2017-06-19 CA CA3028158A patent/CA3028158A1/en active Pending
-
2018
- 2018-11-30 AU AU2018271372A patent/AU2018271372A1/en not_active Abandoned
-
2021
- 2021-06-07 AU AU2021203747A patent/AU2021203747B2/en active Active
-
2022
- 2022-12-23 JP JP2022206177A patent/JP2023052051A/ja active Pending
-
2023
- 2023-06-13 US US18/334,074 patent/US20240191223A1/en active Pending
- 2023-06-13 US US18/334,046 patent/US20230383282A1/en active Pending
- 2023-06-16 JP JP2023098872A patent/JP2023134453A/ja active Pending
- 2023-06-27 AU AU2023204078A patent/AU2023204078A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
EP3455357A1 (en) | 2019-03-20 |
KR20190019168A (ko) | 2019-02-26 |
AU2023204078A1 (en) | 2023-07-13 |
JP2023052051A (ja) | 2023-04-11 |
AU2021203747B2 (en) | 2023-11-09 |
AU2018271372A1 (en) | 2019-01-17 |
CA3028158A1 (en) | 2017-12-21 |
JP2019524072A (ja) | 2019-09-05 |
AU2017283713B2 (en) | 2021-04-08 |
US20230383282A1 (en) | 2023-11-30 |
KR20230156150A (ko) | 2023-11-13 |
WO2017219027A1 (en) | 2017-12-21 |
US11788083B2 (en) | 2023-10-17 |
JP2023134453A (ja) | 2023-09-27 |
AU2017283713A1 (en) | 2018-12-20 |
US20190359971A1 (en) | 2019-11-28 |
US20240191223A1 (en) | 2024-06-13 |
CN109642231A (zh) | 2019-04-16 |
AU2021203747A1 (en) | 2021-07-01 |
JP7267013B2 (ja) | 2023-05-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR112018076190A2 (pt) | ortólogos e sistemas crispr tipo vi | |
AU2018251801A8 (en) | Novel type VI CRISPR orthologs and systems | |
MX2020011939A (es) | Nuevas enzimas y sistemas crispr. | |
EP4361261A3 (en) | Novel cas13b orthologues crispr enzymes and systems | |
AU2018236742B2 (en) | Compositions of adenosine deaminase-2 (ada2), variants thereof and methods of using same | |
AU2018320870A1 (en) | RNA targeting methods and compositions | |
EP3645054A4 (en) | COMPOSITIONS BASED ON CRISPR / CAS-ADENIN-DEAMINASE, SYSTEMS AND METHODS FOR TARGETED NUCLEIC ACID EDITING | |
WO2017127807A8 (en) | Crystal structure of crispr cpf1 | |
EP3692145A4 (en) | NUCLEIC ACID TARGETED EDITING SYSTEMS, METHODS AND COMPOSITIONS | |
EP3684397A4 (en) | SYSTEMS, METHODS AND COMPOSITIONS FOR TARGETED EDITION OF NUCLEIC ACIDS | |
EP3701025A4 (en) | SYSTEMS, PROCEDURES AND COMPOSITIONS FOR TARGETED NUCLEIC ACID EDITING | |
EA201892489A1 (ru) | Число мишеней для мультиплексной оптимизированной амплификации с несоответствиями (moma) | |
MX2020006072A (es) | Métodos y composiciones relacionados con cpf1 para la edición genica. | |
EP3701042A4 (en) | SYSTEMS, PROCEDURES AND COMPOSITIONS FOR TARGETED NUCLEIC ACID EDITING | |
WO2016154127A3 (en) | Compositions and methods for treating hypertriglyceridemia | |
EA201991555A1 (ru) | Композиции и способы усиления или увеличения продукции ifn i типа | |
BR112018010805A2 (pt) | mutantes de proteína f de rsv | |
WO2018109170A3 (en) | Il-11ra antibodies | |
MX2018001497A (es) | Proteinas de fusion de factor ix y metodos para producirlas y usarlas. | |
WO2015191693A3 (en) | Method for gene editing | |
WO2015023975A8 (en) | Compositions and methods for modulating rna | |
SG10201803042PA (en) | Anti-tim-3 antibodies | |
BR112019000693A2 (pt) | composições de muc1- car e métodos para uso | |
WO2018094356A3 (en) | Compositions and methods for target nucleic acid modification | |
IL266131A (en) | Compositions for reprogramming cells into dendritic cells or antigen-presenting cells, methods and uses thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] | ||
B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |