CN1192239A - α-hordothionin的高甲硫氨酸衍生物 - Google Patents
α-hordothionin的高甲硫氨酸衍生物 Download PDFInfo
- Publication number
- CN1192239A CN1192239A CN96195938A CN96195938A CN1192239A CN 1192239 A CN1192239 A CN 1192239A CN 96195938 A CN96195938 A CN 96195938A CN 96195938 A CN96195938 A CN 96195938A CN 1192239 A CN1192239 A CN 1192239A
- Authority
- CN
- China
- Prior art keywords
- sequence
- cell
- met
- methionine
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000002741 methionine derivatives Chemical class 0.000 title description 3
- 229930182817 methionine Natural products 0.000 claims abstract description 34
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims abstract description 28
- 108090000623 proteins and genes Proteins 0.000 claims description 58
- 241000196324 Embryophyta Species 0.000 claims description 37
- 102000004169 proteins and genes Human genes 0.000 claims description 33
- 230000014509 gene expression Effects 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 14
- 241000894006 Bacteria Species 0.000 claims description 8
- 235000013311 vegetables Nutrition 0.000 claims description 8
- 239000013612 plasmid Substances 0.000 claims description 7
- 241001233957 eudicotyledons Species 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 230000001965 increasing effect Effects 0.000 claims description 4
- 244000020551 Helianthus annuus Species 0.000 claims description 3
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 3
- 240000004658 Medicago sativa Species 0.000 claims description 3
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 2
- 240000003768 Solanum lycopersicum Species 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 125000000539 amino acid group Chemical group 0.000 claims 7
- 244000061176 Nicotiana tabacum Species 0.000 claims 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims 1
- 235000009973 maize Nutrition 0.000 claims 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 abstract description 21
- 101710103241 Alpha-hordothionin Proteins 0.000 abstract description 5
- 238000006467 substitution reaction Methods 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 description 30
- 210000004027 cell Anatomy 0.000 description 29
- 229940024606 amino acid Drugs 0.000 description 26
- 235000001014 amino acid Nutrition 0.000 description 26
- 235000018102 proteins Nutrition 0.000 description 24
- 150000001875 compounds Chemical class 0.000 description 9
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 8
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 6
- 235000020776 essential amino acid Nutrition 0.000 description 6
- 108020004999 messenger RNA Proteins 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 239000004475 Arginine Substances 0.000 description 5
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 5
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- 108700005078 Synthetic Genes Proteins 0.000 description 4
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 4
- 239000004473 Threonine Substances 0.000 description 4
- 239000013599 cloning vector Substances 0.000 description 4
- 239000003797 essential amino acid Substances 0.000 description 4
- SMCGQGDVTPFXKB-XPUUQOCRSA-N Ala-Gly-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@H](C)N SMCGQGDVTPFXKB-XPUUQOCRSA-N 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 108010004073 cysteinylcysteine Proteins 0.000 description 3
- 108010069495 cysteinyltyrosine Proteins 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 101100062433 Arabidopsis thaliana DHDPS1 gene Proteins 0.000 description 2
- PSOPJDUQUVFSLS-GUBZILKMSA-N Arg-Met-Cys Chemical compound CSCC[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N PSOPJDUQUVFSLS-GUBZILKMSA-N 0.000 description 2
- HGGIYWURFPGLIU-FXQIFTODSA-N Asn-Met-Cys Chemical compound SC[C@@H](C(O)=O)NC(=O)[C@H](CCSC)NC(=O)[C@@H](N)CC(N)=O HGGIYWURFPGLIU-FXQIFTODSA-N 0.000 description 2
- GEEXORWTBTUOHC-FXQIFTODSA-N Cys-Arg-Ser Chemical compound C(C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CS)N)CN=C(N)N GEEXORWTBTUOHC-FXQIFTODSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- PHONXOACARQMPM-BQBZGAKWSA-N Gly-Ala-Met Chemical compound [H]NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCSC)C(O)=O PHONXOACARQMPM-BQBZGAKWSA-N 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- PINHPJWGVBKQII-SRVKXCTJSA-N Lys-Leu-Cys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CCCCN)N PINHPJWGVBKQII-SRVKXCTJSA-N 0.000 description 2
- CTJUSALVKAWFFU-CIUDSAMLSA-N Lys-Ser-Cys Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)O)N CTJUSALVKAWFFU-CIUDSAMLSA-N 0.000 description 2
- 108010003571 Nut Proteins Proteins 0.000 description 2
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
- 241000607715 Serratia marcescens Species 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- QHUWWSQZTFLXPQ-FJXKBIBVSA-N Thr-Met-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(=O)NCC(O)=O QHUWWSQZTFLXPQ-FJXKBIBVSA-N 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 239000011593 sulfur Substances 0.000 description 2
- 238000001308 synthesis method Methods 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 108010036387 trimethionine Proteins 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- RWWPBOUMKFBHAL-FXQIFTODSA-N Arg-Asn-Cys Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(O)=O RWWPBOUMKFBHAL-FXQIFTODSA-N 0.000 description 1
- AHPWQERCDZTTNB-FXQIFTODSA-N Arg-Cys-Cys Chemical compound C(C[C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CS)C(=O)O)N)CN=C(N)N AHPWQERCDZTTNB-FXQIFTODSA-N 0.000 description 1
- SVHRPCMZTWZROG-DCAQKATOSA-N Arg-Cys-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CS)NC(=O)[C@H](CCCN=C(N)N)N SVHRPCMZTWZROG-DCAQKATOSA-N 0.000 description 1
- OMKZPCPZEFMBIT-SRVKXCTJSA-N Arg-Met-Arg Chemical compound NC(=N)NCCC[C@H](N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O OMKZPCPZEFMBIT-SRVKXCTJSA-N 0.000 description 1
- ISVACHFCVRKIDG-SRVKXCTJSA-N Arg-Val-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O ISVACHFCVRKIDG-SRVKXCTJSA-N 0.000 description 1
- UHGUKCOQUNPSKK-CIUDSAMLSA-N Asn-Leu-Cys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CC(=O)N)N UHGUKCOQUNPSKK-CIUDSAMLSA-N 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 241000209763 Avena sativa Species 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- HJGUQJJJXQGXGJ-FXQIFTODSA-N Cys-Met-Ser Chemical compound CSCC[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CS)N HJGUQJJJXQGXGJ-FXQIFTODSA-N 0.000 description 1
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- GZUKEVBTYNNUQF-WDSKDSINSA-N Gly-Ala-Gln Chemical compound NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(O)=O GZUKEVBTYNNUQF-WDSKDSINSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- CKRRYCJKCRZOCU-GUBZILKMSA-N Met-Cys-Met Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@H](C(O)=O)CCSC CKRRYCJKCRZOCU-GUBZILKMSA-N 0.000 description 1
- KKXGLCPUAWODHF-GUBZILKMSA-N Met-Met-Cys Chemical compound N[C@@H](CCSC)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CS)C(O)=O KKXGLCPUAWODHF-GUBZILKMSA-N 0.000 description 1
- VWWGEKCAPBMIFE-SRVKXCTJSA-N Met-Met-Met Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCSC)C(O)=O VWWGEKCAPBMIFE-SRVKXCTJSA-N 0.000 description 1
- WRXOPYNEKGZWAZ-FXQIFTODSA-N Met-Ser-Cys Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CS)C(O)=O WRXOPYNEKGZWAZ-FXQIFTODSA-N 0.000 description 1
- 102000005877 Peptide Initiation Factors Human genes 0.000 description 1
- 108010044843 Peptide Initiation Factors Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 241000201976 Polycarpon Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- RFKVQLIXNVEOMB-WEDXCCLWSA-N Thr-Leu-Gly Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)O)N)O RFKVQLIXNVEOMB-WEDXCCLWSA-N 0.000 description 1
- 235000019714 Triticale Nutrition 0.000 description 1
- COYSIHFOCOMGCF-UHFFFAOYSA-N Val-Arg-Gly Natural products CC(C)C(N)C(=O)NC(C(=O)NCC(O)=O)CCCN=C(N)N COYSIHFOCOMGCF-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 230000003570 biosynthesizing effect Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000005094 computer simulation Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- YQYJSBFKSSDGFO-FWAVGLHBSA-N hygromycin A Chemical compound O[C@H]1[C@H](O)[C@H](C(=O)C)O[C@@H]1Oc1ccc(\C=C(/C)C(=O)N[C@@H]2[C@@H]([C@H]3OCO[C@H]3[C@@H](O)[C@@H]2O)O)cc1O YQYJSBFKSSDGFO-FWAVGLHBSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- XUWPJKDMEZSVTP-LTYMHZPRSA-N kalafungina Chemical compound O=C1C2=C(O)C=CC=C2C(=O)C2=C1[C@@H](C)O[C@H]1[C@@H]2OC(=O)C1 XUWPJKDMEZSVTP-LTYMHZPRSA-N 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003990 molecular pathway Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- -1 phosphonothricin Chemical compound 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 241000228158 x Triticosecale Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8253—Methionine or cysteine
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
通过利用甲硫氨酸残基进行位置特异性替换产生α－Hordothionin的衍生物,在植物中提供丰富的苏氨酸。
Description
技术领域
本发明涉及饲料制剂的改善，本发明尤其涉及能够在植物中提供较高百分含量的甲硫氨酸的α-hordothionin衍生物。发明背景
使用饲料制剂为动物提供对其生长起关键作用的必需营养物质是必要的。然而，农作物植物一般说来是低营养质量的食物来源，因为它们含有低比例的某些氨基酸，而这些氨基酸对动物来说是必不可少的，但又无法自身合成。
多年以来研究者们曾经试图通过杂交计划来改善重要的农作物蛋白质中必需氨基酸的平衡。随着更多地有关贮藏蛋白质和编码这些蛋白质的基因表达的知识得到了解，以及随着适用于更多种类植物的转化系统的发展，利用分子途径改善种子蛋白质营养质量为比较传统的途径提供了另外的选择。因此，在某一给定的农作物中的特殊氨基酸水平可以通过生物工程学方法得到增加。
一种可供选择的方法是在大量的足以免除食物或饲料添加剂的水平上来表达一种含有想要的氨基酸组合物的异源蛋白质。例如，许多富含含硫氨基酸的种子蛋白质已经得到鉴定。这些蛋白质很好地表达的关键涉及具有种子特异性启动子的有效的表达盒。不仅基因控制区域必须指导高水平的信使RNA的合成，而且信使RNA必须被翻译成稳定的蛋白质。
在动物营养必需的氨基酸中，农作物经常缺乏的是甲硫氨酸，苏氨酸和赖氨酸。通过育种，突变筛选和/或改变农作物中积累的贮藏蛋白质的组成来增加这些自由氨基酸的水平的努力很少曾取得成功。通常转基因贮藏蛋白质的表达水平太低。菜豆蛋白启动的巴西坚果2S表达盒是一个有效的嵌合种子特异性基因的例子，然而，虽然巴西坚果蛋白质中增加了总的甲硫氨酸和结合甲硫氨酸的数量，由此改善了营养价值，但是对于积累在种子中的甲硫氨酸总量似乎有一个阈值限制。其种子作为甲硫氨酸的来源仍然是不充足的。
另一种通过改变含有希望得到的氨基酸的蛋白质水平来增加特殊氨基酸水平的可供选择的方法是对氨基酸的生物合成进行修饰。重组DNA和基因转移技术已被用于改变氨基酸生物合成途径中催化关键步骤的酶的活性。Glassman，美国专利申请号NO.5,258,300；Galili等，欧洲专利申请号NO.485970；(1992)；在此全文引入作为参考。然而，种子中氨基酸水平的修饰并不总是与结合这些氨基酸的蛋白质水平的变化有关。Burrow等，Mol.Gen.Genet.Vol.241；pp.431-439；(1993)；在此全文引入作为参考。虽然通过选择DHDPS突变体或者将大肠杆菌(E.Coli)DHDPS在植物中进行表达能够使叶子中游离氨基酸的水平得到显著增加，但仍显示出这些改变只能增加代表着占氨基酸总量大约90％左右的靶结合氨基酸，对种子的营养价值只有极小的影响。
基于前述的原因，有必要寻找方法增加植物种子中甲硫氨酸、赖氨酸和苏氨酸等必需氨基酸的水平。
因此，本发明的目的之一正是为通过植物遗传修饰来增加植物中必需氨基酸甲硫氨酸的水平提供方法。
本发明更深入的目的是提供与同类种子的野生型相比，具有更高水平必需氨基酸甲硫氨酸的种子作为食物和/或饲料。发明内容
已经发现一类化合物，α-hordothionins，可被修饰以增加它们的甲硫氨酸含量。α-hordothionin是一种已经得到充分认识的45个氨基酸的蛋白质，它可以从大麦(Hordeum vulgare)种子中分离得到。其分子由于8个半胱氨酸残基形成的4个二硫键而保持稳定，序列I.D.NO.1提供了其氨基酸序列。在天然形式下，些蛋白质尤其富含精氨酸和赖氨酸残基，分别含有5个残基(10％)，但它缺乏必需氨基酸甲硫氨酸。
此蛋白质已被人工合成，并且其三维结构已通过计算机模型得到确定。蛋白质模型表明10个带电荷的残基(位置5，10，17，19和30上的精氨酸及位置1，23，32，38和45上的赖氨酸)都出现在分子的表面。极性氨基酸(位置11上的天冬酰胺，位置22上的谷氨酰胺和位置41上的苏氨酸)的侧链也出现在分子的表面上，再者，疏水氨基酸(例如位置8，15，24和33上的亮氨酸及位置18上的缬氨酸的侧链)也是靠近溶剂的。
蛋白质的三维模型显示出位置10上的精氨酸残基对于保持合适的三维结构和通过其与蛋白质C-末端残基的氢键作用进行合理的折叠是非常关键的。在这一位点上替换成甲硫氨酸会打破涉及位置10上的精氨酸，位置2上的丝氨酸和位置45上的赖氨酸的氢键，导致结构稳定性的破坏。具有这种替换的合成多肽无法正确折叠，更加支持了这一分析。位置10上的精氨酸残基的保留提供了正确折叠的蛋白质。
由于甲硫氨酸是一个疏水氨基酸，位置8，15，和33上的亮氨酸及位置18上的缬氨酸等表面疏水氨基酸可被甲硫氨酸替换。位置11上的天冬酰胺，位置22上的谷氨酰胺及位置41上的苏氨酸等表面极性氨基酸也可被甲硫氨酸替换。形成的化合物具备序列I.D.NO.2中显示的序列，其分子通过固相多肽合成法进行合成并折叠成稳定的结构。它含有7个甲硫氨酸残基(15.5％)，并且包括8个半胱氨酸，被修饰后的蛋白质含有33％的含硫氨基酸。
虽然序列I.D.NO.2在本发明中进行了绘图说明，但并不打算就此为止。甲硫氨酸的替换还可以在含有带电荷的氨基酸的位置上进行，只有位置10上的精氨酸对于通过其与位置2上的丝氨酸及位置45上的赖氨酸形成的氢键网络来保持蛋白质的结构是极关键的。因而，我们可以把位置1，23，32和/或38上的赖氨酸及位置5，17，19和/或30上的精氨酸替换为甲硫氨酸，最后形成的化合物具有序列I.D.NO.3中显示的序列。
化合物的合成按照本领域中熟知的多肽合成方法进行，因而不构成本发明的一部分。正如Rao等于Int.J.Pep.Prot.Res.；Vol.40；pp508-515；(1992)上发表的那样，在体外，化合物利用包含hbtu[2-(1h-苯并三唑-1-基)-1，1，3，3-tetramethyluroniumhexafluorophosphate的fastmocTM化学，在应用生物系统431a型多肽合成仪上进行合成，在此全文引入作为参考。多肽按照标准规程进行切割，通过反相色谱利用标准方法进行纯化。每个多肽的氨基酸顺序利用自动Edman降解在应用生物系统477a蛋白质顺序仪/120a pth分析仪上进行测定。然而，更优选的是本发明的化合物在细菌或植物细胞内进行合成，这些细胞已经被转化插入了含有合成基因的表达盒，此合成基因在被转录和翻译时将产生出所希望的化合物。这种空的表达盒是为人熟知的，它为植物或细菌表达所希望的序列提供了合适的调控序列，同时合成基因，RNA或DNA，的核苷酸序列可以方便地利用标准的参照系统从蛋白质的氨基酸顺序推导出来。此合成基因将优选地使用植物优先的密码子的增强所希望的蛋白质的表达。工业应用性
以下的描述进一步举例说明了本发明的组合物以及制造和使用它们的方法。然而，应当知道的是被本领域中的普通技术人员所了解的相当的其它方法也可以使用。植物
编码这些化合物的基因可以插入到合适的表达盒中，并被引入到植物细胞中。如此，本方法特别优选的实施方案涉及将一段DNA序列以及在植物中具有活性的转录起始因子和启动子顺序插入到植物的基因组中，此DNA序列在适当的阅读框架下，可以编码本发明中的化合物。在此调控顺序控制下的DNA序列的转录和翻译导致了此蛋白质序列在植物组织中的高效表达，从而产生出超量的蛋白质。
根据本发明的方法，优选的转化植物是谷类作物，包括玉米、黑麦、大麦、小麦、高梁、燕麦、小米、大米、黑小麦、向日葵、紫苜蓿、油菜籽和大豆。
此时可以制备编码适当氨基酸顺序的合成DNA序列，并且此合成DNA序列可以被插入到合适的植物表达盒中。
同样地，许多植物表达盒和载体在本领域中是人所共知的。“表达盒”这个术语的意思是指一个包括起始、启动子和终止子序列的一整套的控制序列，当它们插入到一个具有适当的阅读框架的结构基因之侧翼时，就会在植物的细胞中起作用。表达盒通常并且优选地含有各种可供选择的适合于切开和插入任何希望的结构基因的限制性位点。重要的是克隆的基因必须有一个在正确阅读框架内的结构序列的起始密码子。
另外，植物表达盒优选地应在某一末端包含一个强组成型启动子序列，使基因得以高频率地转录，在另一末端含有一个多聚腺核苷酸识别序列，以使信使RNA得到恰当的加工和转运。Pioneer Hi-BredInternational，Inc.Johnston，IA的Beach等人开发的pPHI 414质粒就是这样一个优选的(空的)表达盒的例子，本发明的cDNA可被插入到此表达盒中，pPHI 414质粒公开于美国专利申请号NO.07/785,648，(1991)上，在此全文引入作为参考。高度优选的植物表达盒将被设计包含有一个或多个选择标记基因，例如卡那霉素抗性或除草剂耐受性的基因。
术语“载体”的意思在此是指一段DNA序列，它可以在宿主细胞内复制并表达一个外来的基因。载体通常含有一个或者多个内切酶识别位点，这些位点可以用合适的酶以一种可预测的方式进行切割，这种载体优选地被构建成含有附加的带有抗生素或除草剂抗性的结构基因序列，作为鉴定和分离转化后的细胞的标记物。优选的标记物/筛选剂包括卡那霉素、chlorosulfuron、phosphonothricin，潮霉素和氨甲蝶呤。细胞中载体的外来遗传物质被功能性地表达后，此细胞即已被载体转化并且被称为“转化株”。
一种特别优选的载体是质粒，质粒是一个存在于细胞染色体外的环状双链DNA分子。
如上所述，无论编码兴趣基因的是基因组基因还是cDNA都可用于本发明中。感兴趣的载体也可以由部分cDNA克隆和部分基因组基因构建而成。当兴趣基因被分离出来后，对基因进行遗传构建使之含有必需的调控顺序，从而使基因在宿主细胞内能够有效地表达。根据本发明，遗传构建物将含有(a)一段首要的编码感兴趣的蛋白质或特征的遗传序列，(b)可操作地连接在感兴趣的结构基因任何一端的一个或多个调控顺序。通常调控顺序将选自启动子种终止子之一。调控顺序可以来源于同种或异种。
可以用在遗传序列中的启动子包括NOS，OCS和CaMV启动子。
可使用的有效的植物启动子是高产植物启动子。在本发明中可使用的高产植物启动子包括叶绿素α-β结合蛋白启动子和大豆核酮糖-1，5-二磷酸羧化酶小亚基(ss)的启动子，参看例如Berry-Lowe等，J.Molecular and App.Gen.，Vol.1；pp.483-498；(1982)，在此全文引入作为参考。据悉这两个启动子在植物细胞中是光诱导的。参看例如An Agricultural Perspective，A.Cashmore，Pelham，New York；1983，pp29-38，G.Coruzzi等，J.Biol.Chem.，Vol.258；p.1399；(1983)；以及P.Dunsmuir等，J.Molecular and App.Gen.；Vol 2；p.285；(1983)；都在此全文引入作为参考。
包含被可操作地连接在所希望的控制顺序上的本发明的蛋白质结构基因的表达盒可以被连接入适当的克隆载体中。一般使用源自与宿主细胞相容的物种的含有复制和控制顺序的质粒或病毒(噬菌体)载体。克隆载体通常将带有一个复制起点，以及能够在转化后的宿主细胞中提供表型选择标记的特殊基因，通常使用能赋予抗生素或挑选的除草剂抗性的基因。遗传物质被引入靶细胞后，成功地被转化的细胞和/或细胞克隆可以通过这些标记进行筛选从而得到分离。
通常为了增加克隆载体的拷贝数量，在本发明的实践中将使用一个中间宿主细胞。有了增加的拷贝数量，含有兴趣基因的载体就可以被大量地分离以便于引入到所希望的植物细胞中。可用于本发明实践中的宿主细胞包括原核生物，包括细菌宿主例如大肠杆菌(E.Coli)，鼠伤寒沙门氏菌(S.typhimurium)，和粘质沙雷氏菌(Serratia marcescens)。真核宿主例如酵母或丝状真菌也可以用于本发明中。由于这些宿主也是微生物，必须保证在载体中使用无法引起细菌本身蛋白质表达的植物启动子。
然后，分离的克隆载体将利用适当的技术引入到植物细胞中，这些技术包括电穿孔(在原生质体中)，逆病毒、粒子辐射、和显微注射到单子叶或双子叶植物的细胞或组织培养物中以提供转化的植物细胞，这些细胞含有作为外来DNA的至少一个拷贝的植物表达盒DNA序列。优选地，单子叶的品种将选自玉米、高梁、小麦或大米，而双子叶的品种将选自大豆、紫苜蓿、油菜籽、向日葵或蕃茄。利用已知的技术，原生质体可以被再生，细胞和组织培养物也可被再生从而生成完整的可繁殖的植物，此种植物携带并且表达本发明中蛋白质的基因。因此，本发明高度优选的实施方案是一种转化后的玉米植物，其细胞中含有作为外来DNA的至少一个拷贝的本发明的表达盒的DNA序列。
普通技术人员将会欣赏的是这里提供的植物载体可被引入到根癌土壤杆菌(Agrobacterium tumefaciens)中，然后利用其将载体转入到易感植物细胞中，这些植物细胞主要来自双子叶的物种。至此，本发明提供了增加根癌土壤杆菌一易感的双子叶植物中甲硫氨酸水平的一个方法，其中通过利用其质粒已经过修饰因而包含有一个本发明中的植物表达盒的根癌土壤杆菌来感染细胞，从而将表达盒引入到细胞中去。
序列表(1)一般资料：
(i)申请人：Pioneer Hi-Bred International.Inc.
(ii)发明名称：Alpha-Hordothionin的高甲硫氨酸衍生物
(iii)序列数目：3
(iv)通讯地址：
(A)称呼：Pioneer Hi-Bred International.Inc.
(B)街道：700Capital Square，400 Locust Street.
(C)城市：Des Moines
(D)州：Iowa.
(E)国家：United States of America.
(F)邮编：(ZIP)50309.(v)计算机可读形式：
(A)媒体类型：软盘
(B)计算机：IBM兼容
(C)操作系统：PC-DOS/MS-DOS
(D)软件：Patent In Release#1.0，Version#1.25(vi)通用申请资料：
(A)申请号：PCT
(B)存档日期：
(C)分类：(viii)律师/事务所资料：
(A)姓名：Simon，Soma G.
(B)注册号：37,444
(C)参考文献/摘要号：355-US(ix)电信资料：
(A)电话：515-248-4896
(B)电传：515-248-4844(2)SEQ ID NO.1的资料：(i)序列特征：
(A)长度：45个氨基酸
(B)类型：氨基酸
(C)拓扑结构：线性(xi)序列描述：SEQ ID NO.1：Lys Ser Cys Cys Arg Ser Thr Leu Gly Arg Asn Cys Tyr Asn LeuCys1 5 10 15Arg Val Arg Gly Ala Gln Lys Leu Cys Ala Gly Val Cys Arg CysCys
20 25 30Leu Thr Ser Ser Gly Lys Cys Pro Thr Gly Phe Pro Lys
35 40 45(2)SEQ ID.NO.2的资料：(i)序列特征：
(A)长度：45个氨基酸
(B)类型：氨基酸
(C)拓扑结构：线性(xi)序列描述：SEQ ID NO.2：Lys Ser Cys Cys Arg Ser Thr Met Gly Arg Met Cys Tyr ASn MetCys1 5 10 15Arg Met Arg Gly Ala Met Lys Leu Cys Ala Gly Val Cys Arg CysLys
20 25 30Met Thr Ser Ser Gly Lys Cys Pro Met Gly Phe Pro Lys
35 40 45(2)SEQ ID NO.3的资料：(i)序列特征：
(A)长度：45个氨基酸
(B)类型：氨基酸
(C)拓扑结构：线性(xi)序列描述：SEQ ID NO.3：Met Ser Cys Cys Met Ser Thr Met Gly Arg Met Cys Tyr Asn MetCys1 5 10 15Met Met Met Gly Ala Met Met Met Cys Ala Gly Val Cys Met CysMet
20 25 30Met Thr Ser Ser Gly Met Cys Pro Met Gly Phe Pro Lys
35 40 45
Claims (20)
1.一种具有序列I.D.NO.3之序列的蛋白质，其中位置1，5，8，11，15，17，18，19，22，23，24，30，32，33，38和41上的一个或多个位置上的氨基酸残基是甲硫氨酸，并且这些位置上的余留残基为序列I.D.NO.1中相应位置上的残基。
2.权利要求1中的蛋白质，其中位置8，11，15，18，22，33和41上的一个或多个氨基酸残基是甲硫氨酸。
3.权利要求2中的蛋白质，其中位置8，11，15，18，22，33和41上至少有3个氨基酸残基是甲硫氨酸。
4.权利要求3中的蛋白质，其中位置8，11，15，18，22，33和41上至少有5个氨基酸残基是甲硫氨酸。
5.一段编码具有序列I.D.NO.2之序列的蛋白质的核苷酸序列，其中位置1，5，8，11，15，17，18，19，22，23，24，30，32，33，38和41上的一个或多个位置上的氨基酸残基是甲硫氨酸，并且这些位置上的余留残基为序列I.D.NO.1中相应位置上的残基。
6.一段编码具有序列I.D.NO.2之序列的蛋白质的RNA序列，其中位置1，5，8，11，15，17，18，19，22，23，24，30，32，33，38和41上的一个或多个位置上的氨基酸残基是甲硫氨酸，并且这些位置上的余留残基为序列I.D.NO.1中相应位置上的残基。
7.一段编码具有序列I.D.NO.3之序列的蛋白质的DNA序列，其中位置1，5，8，11，15，17，18，19，22，23，24，30，32，33，38和41上的一个或多个位置上的氨基酸残基是甲硫氨酸，并且这些位置上的余留残基为序列I.D.NO.1中相应位置上的残基。
8.一种含有权利要求7中的DNA序列的表达盒，被可操作地连接到植物调控顺序上，从而引起DNA序列在植物细胞中的表达。
9.一种含有一个权利要求8中表达盒的细菌转化载体，被可操作地连接到细菌表达调控顺序上，从而引起此表达盒在细菌中的复制。
10.细菌细胞，含有作为外来质粒的至少一个拷贝的权利要求9中的细菌转化载体。
11.转化的植物细胞，含有至少一个拷贝的权利要求8中的表达盒。
12.权利要求11中的转化植物细胞，其中细胞属于单子叶的物种。
13.权利要求12中的转化植物细胞，其中细胞选自玉米、高梁、小麦和大米细胞之一。
14.权利要求11中的转化细胞，其中细胞属于双子叶的物种。
15.权利要求14中的转化细胞，其中细胞选自大豆、紫苜蓿、油菜籽、向日葵、烟草和蕃茄细胞之一。
16.一种包括权利要求13中细胞的玉米细胞或组织培养物。
17.一种增加植物细胞或种子中甲硫氨酸含量的方法，包括在细胞或种子中表达权利要求1中蛋白质的步骤。
18.一种权利要求17中的方法，其中植物是双子叶的植物。
19.一种权利要求17中的方法，其中植物是单子叶的植物。
20.一种权利要求19中的方法，其中植物种子中的甲硫氨酸含量得到增加。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US46044095A | 1995-06-02 | 1995-06-02 | |
| US08/460,440 | 1995-06-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1192239A true CN1192239A (zh) | 1998-09-02 |
Family
ID=23828714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96195938A Pending CN1192239A (zh) | 1995-06-02 | 1996-05-31 | α-hordothionin的高甲硫氨酸衍生物 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5885802A (zh) |
| EP (1) | EP0832235A1 (zh) |
| JP (1) | JPH11506329A (zh) |
| CN (1) | CN1192239A (zh) |
| AR (1) | AR004938A1 (zh) |
| AU (1) | AU707354B2 (zh) |
| BR (1) | BR9609299A (zh) |
| CA (1) | CA2222600A1 (zh) |
| HU (1) | HUP9900878A2 (zh) |
| MX (1) | MX9709352A (zh) |
| PL (1) | PL323635A1 (zh) |
| WO (1) | WO1996038563A1 (zh) |
Families Citing this family (195)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6080913A (en) * | 1996-09-25 | 2000-06-27 | Pioneer Hi-Bred International, Inc. | Binary methods of increasing accumulation of essential amino acids in seeds |
| AR017831A1 (es) | 1997-12-10 | 2001-10-24 | Pioneer Hi Bred Int | Metodo para alterar la composicion de aminoacidos de una proteina nativa de interes, proteina elaborada, y polinucleotido |
| US7053282B1 (en) | 1998-02-09 | 2006-05-30 | Pioneer Hi-Bred International, Inc. | Alteration of amino acid compositions in seeds |
| MXPA04002817A (es) | 2001-09-27 | 2004-07-05 | Pioneer Hi Bred Int | Polinucleotidos de fitato y metodos de uso. |
| EP1572927A4 (en) | 2002-04-08 | 2007-10-17 | Pioneer Hi Bred Int | REINFORCED EXTRACTION OF SILK UNDER STRESS |
| EP2216405A1 (en) | 2002-05-03 | 2010-08-11 | Monsanto Technology LLC | Speed specific USP promoters for expressing genes in plants |
| EP2275536A1 (en) | 2002-08-06 | 2011-01-19 | Verdia, Inc. | AP1 amine oxidase variants |
| US7078234B2 (en) | 2002-12-18 | 2006-07-18 | Monsanto Technology Llc | Maize embryo-specific promoter compositions and methods for use thereof |
| WO2004087878A2 (en) | 2003-03-28 | 2004-10-14 | Monsanto Technology, Llc | Novel plant promoters for use in early seed development |
| CN1863914B (zh) | 2003-04-29 | 2011-03-09 | 先锋高级育种国际公司 | 新的草甘膦-n-乙酰转移酶(gat)基因 |
| CA2529658C (en) | 2003-06-23 | 2017-09-12 | Pioneer Hi-Bred International, Inc. | Engineering single-gene-controlled staygreen potential into plants |
| ES2339559T3 (es) | 2003-12-16 | 2010-05-21 | Pioneer Hi-Bred International, Inc. | Transgenes de supresion de gen dominante y metodos de uso de los mismos. |
| US20070169227A1 (en) | 2003-12-16 | 2007-07-19 | Pioneer Hi-Bred International Inc. | Dominant Gene Suppression Transgenes and Methods of Using Same |
| CA2571585C (en) | 2004-06-30 | 2011-11-08 | Pioneer Hi-Bred International, Inc. | Methods of protecting plants from pathogenic fungi |
| WO2006014316A1 (en) | 2004-07-02 | 2006-02-09 | Pioneer Hi-Bred International, Inc. | Antifungal polypeptides |
| CA2592894C (en) | 2004-12-28 | 2013-08-27 | Pioneer Hi-Bred International, Inc. | Improved grain quality through altered expression of gamma-zein protein |
| US20090031440A1 (en) | 2005-02-26 | 2009-01-29 | Basf Plant Science Gmbh | Expression Cassettes for Seed-Preferential Expression in Plants |
| ATE552343T1 (de) * | 2005-04-19 | 2012-04-15 | Basf Plant Science Gmbh | Endosperm-spezifische expression und/oder expression in keimenden embryos monokotyledoner pflanzen |
| WO2006111541A2 (en) | 2005-04-20 | 2006-10-26 | Basf Plant Science Gmbh | Expression cassettes for seed-preferential expression in plants |
| EP1882037A2 (en) | 2005-05-10 | 2008-01-30 | BASF Plant Science GmbH | Expression cassettes for seed-preferential expression in plants |
| EP2261362A3 (en) | 2005-05-25 | 2011-04-20 | Pioneer Hi-Bred International Inc. | Methods for improving crop plant architecture and yield |
| EP1907553B1 (en) | 2005-07-18 | 2012-08-22 | Pioneer Hi-Bred International Inc. | Modified frt recombination sites and methods of use |
| CA2895745A1 (en) | 2006-02-09 | 2007-08-16 | Pioneer Hi-Bred International, Inc. | Genes for enhancing nitrogen utilization efficiency in crop plants |
| CA2638739A1 (en) | 2006-03-01 | 2007-09-13 | Pioneer Hi-Bred International, Inc. | Compositions related to the quantitative trait locus 6 (qtl6) in maize and methods of use |
| US20070214515A1 (en) | 2006-03-09 | 2007-09-13 | E.I.Du Pont De Nemours And Company | Polynucleotide encoding a maize herbicide resistance gene and methods for use |
| MX2008013354A (es) | 2006-04-19 | 2008-11-10 | Pioneer Hi Bred Int | Moleculas de polinucleotidos aislados que corresponden a alelos mutantes y tipo salvaje del gen de maiz d9, y metodos para usarlas. |
| DE602007012343D1 (de) | 2006-05-16 | 2011-03-17 | Du Pont | Antimykotische polypeptide |
| US7951995B2 (en) | 2006-06-28 | 2011-05-31 | Pioneer Hi-Bred International, Inc. | Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof |
| US7777102B2 (en) * | 2007-02-08 | 2010-08-17 | University Of Tennessee Research Foundation | Soybean varieties |
| BRPI0808008B1 (pt) | 2007-02-16 | 2016-08-09 | Basf Plant Science Gmbh | molécula de ácido nucleico isolada, cassete de expressão, vetor de expressão, métodos para excisão de sequências alvo de uma planta, para produzir uma planta com rendimento aumentado, e/ou tolerância a estresse aumentada, e/ou qualidade nutritional aumentada, e/ou teor de óleo aumentado ou modificado de uma semente ou broto para a planta, e, uso da molécula de ácido nucleico |
| US8847013B2 (en) | 2008-01-17 | 2014-09-30 | Pioneer Hi Bred International Inc | Compositions and methods for the suppression of target polynucleotides from lepidoptera |
| US8367895B2 (en) | 2008-01-17 | 2013-02-05 | Pioneer Hi-Bred International, Inc. | Compositions and methods for the suppression of target polynucleotides from the family aphididae |
| US7935870B2 (en) * | 2008-05-14 | 2011-05-03 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV354718 |
| US7964774B2 (en) * | 2008-05-14 | 2011-06-21 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV384196 |
| US7947877B2 (en) * | 2008-05-14 | 2011-05-24 | Monosanto Technology LLC | Plants and seeds of spring canola variety SCV328921 |
| US8829282B2 (en) * | 2008-05-14 | 2014-09-09 | Monsanto Technology, Llc | Plants and seeds of spring canola variety SCV425044 |
| US20100115662A1 (en) | 2008-10-30 | 2010-05-06 | Pioneer Hi-Bred International Inc. | Manipulation of glutamine synthetases (gs) to improve nitrogen use efficiency and grain yield in higher plants |
| CA2751724A1 (en) | 2009-02-19 | 2010-08-26 | Pioneer Hi-Bred International, Inc. | Blended refuge deployment via manipulation during hybrid seed production |
| CN102395674B (zh) | 2009-04-14 | 2015-07-29 | 先锋国际良种公司 | 调节acc合酶改善低氮条件下的植物产量 |
| AU2010237615B2 (en) | 2009-04-17 | 2013-08-15 | Basf Plant Science Company Gmbh | Plant promoter operable in endosperm and uses thereof |
| US8471100B2 (en) * | 2009-05-15 | 2013-06-25 | University Of Tennessee Research Foundation | Environmental stress-inducible promoter and its application in crops |
| US8466342B2 (en) | 2009-06-09 | 2013-06-18 | Pioneer Hi Bred International Inc | Early endosperm promoter and methods of use |
| US8071848B2 (en) * | 2009-06-17 | 2011-12-06 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV218328 |
| EP2451958A1 (en) | 2009-07-10 | 2012-05-16 | BASF Plant Science Company GmbH | Expression cassettes for endosperm-specific expression in plants |
| EP2456874A1 (en) | 2009-07-24 | 2012-05-30 | Pioneer Hi-Bred International Inc. | The use of dimerization domain component stacks to modulate plant architecture |
| US20110035843A1 (en) | 2009-08-05 | 2011-02-10 | Pioneer Hi-Bred International, Inc. | Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants |
| IN2012DN01359A (zh) | 2009-08-20 | 2015-06-05 | Pioneer Hi Bred Int | |
| CN102549149A (zh) | 2009-08-20 | 2012-07-04 | 先锋国际良种公司 | 在玉蜀黍中功能性表达酵母硝酸盐转运蛋白(ynt1)以改善硝酸盐吸收 |
| US20110054007A1 (en) | 2009-08-28 | 2011-03-03 | E.I. Du Pont De Nemours And Company | Compositions and methods to control insect pests |
| IN2012DN02408A (zh) | 2009-10-02 | 2015-08-21 | Pioneer Hi Bred Int | |
| MX2012004819A (es) | 2009-10-26 | 2012-06-25 | Pioneer Hi Bred Int | Promotor especifico de ovulo somatico y metodos de uso. |
| AU2010325720A1 (en) | 2009-12-03 | 2012-07-19 | Basf Plant Science Company Gmbh | Expression cassettes for embryo-specific expression in plants |
| CN102939383B (zh) | 2009-12-30 | 2015-04-29 | 先锋国际良种公司 | 用于靶向多核苷酸修饰的方法和组合物 |
| AU2010339404B2 (en) | 2009-12-30 | 2016-01-28 | Pioneer Hi-Bred International, Inc. | Methods and compositions for the introduction and regulated expression of genes in plants |
| WO2011082304A1 (en) | 2009-12-31 | 2011-07-07 | Pioneer Hi-Bred International, Inc. | Engineering plant resistance to diseases caused by pathogens |
| CA2786741A1 (en) | 2010-01-06 | 2011-07-14 | Pioneer Hi-Bred International, Inc. | Identification of diurnal rhythms in photosynthetic and non-photosynthetic tissues from zea mays and use in improving crop plants |
| US8148611B2 (en) * | 2010-02-26 | 2012-04-03 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV453784 |
| US8143488B2 (en) * | 2010-02-26 | 2012-03-27 | Monsanto Technoloy LLC | Plants and seeds of spring canola variety SCV470336 |
| US8138394B2 (en) * | 2010-02-26 | 2012-03-20 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV431158 |
| US8581048B2 (en) * | 2010-03-09 | 2013-11-12 | Monsanto Technology, Llc | Plants and seeds of spring canola variety SCV119103 |
| US8153865B2 (en) * | 2010-03-11 | 2012-04-10 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV152154 |
| AR080745A1 (es) | 2010-05-06 | 2012-05-02 | Du Pont | Gen y proteina acc (acido 1-aminociclopropano-1-carboxilico) sintasa 3 de maiz y sus usos |
| MX2012014663A (es) | 2010-06-25 | 2013-01-22 | Du Pont | Composiciones y metodos para mejorar la resistencia al tizon norteño de las hojas en maiz. |
| BR112013003221A2 (pt) | 2010-08-13 | 2019-09-24 | Pioneer Hi Bred Int | polinucleotídeo quimérico, construto de ácido nucleico, célula, planta, explante vegetal, semente transgênica, polipeptídeo quimérico, método de direcionar um polipeptídeo de interesse a um cloroplasto, cassete de expressão, célula vegetal, polipeptídeo isolado |
| CN103154252B (zh) | 2010-08-23 | 2017-05-24 | 先锋国际良种公司 | 防御素变体及其使用方法 |
| BR112013012068B1 (pt) | 2010-11-17 | 2020-12-01 | Pioneer Hi-Bred International, Inc. | método imparcial para prever o fenótipo ou traço de pelo menos uma planta independente |
| WO2012071039A1 (en) | 2010-11-24 | 2012-05-31 | Pioner Hi-Bred International, Inc. | Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof |
| CN103748227A (zh) | 2010-11-24 | 2014-04-23 | 先锋国际良种公司 | 芸苔gat事件dp-073496-4及其鉴定和/或检测组合物和方法 |
| TWI667347B (zh) | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法 |
| US8895716B2 (en) | 2010-12-22 | 2014-11-25 | Pioneer Hi-Bred International, Inc. | Viral promoter, truncations thereof, and methods of use |
| CN103261425B (zh) | 2010-12-22 | 2015-07-15 | 先锋国际良种公司 | 病毒启动子、其截短物以及使用方法 |
| BR112013015515A2 (pt) | 2010-12-28 | 2018-04-24 | Pioneer Hi Bred Int | molécula de ácido nucleico isolada, construto de dna, célula hospedeira, planta transgênica, semente transformada da planta, polipeptídeo isolado com atividade pesticida, composição, método para controlar uma população de praga de lepidóptero, método para matar uma praga de lepidóptero, método para produzir um polipeptídeo com atividade pesticida, planta que tem incorporado de maneira estável em seu genoma um construto de dna, método para proteger uma planta contra uma praga |
| EA030578B1 (ru) | 2011-02-01 | 2018-08-31 | Колорадо Уит Рисерч Фаундейшн, Инк. | РАСТЕНИЯ, УСТОЙЧИВЫЕ К ГЕРБИЦИДАМ, ВОЗДЕЙСТВУЮЩИМ НА АЦЕТИЛ-КоА КАРБОКСИЛАЗУ |
| CN103459601A (zh) | 2011-02-11 | 2013-12-18 | 先锋国际良种公司 | 具有对抗玉米根虫的活性的合成杀昆虫蛋白 |
| US8878007B2 (en) | 2011-03-10 | 2014-11-04 | Pioneer Hi Bred International Inc | Bacillus thuringiensis gene with lepidopteran activity |
| CA2831144A1 (en) | 2011-03-23 | 2012-09-27 | Pioneer Hi-Bred International, Inc. | Methods for producing a complex transgenic trait locus |
| EP2794887A2 (en) | 2011-03-30 | 2014-10-29 | Universidad Nacional Autonoma De Mexico | Mutant bacillus thuringiensis cry genes and methods of use |
| US8513487B2 (en) | 2011-04-07 | 2013-08-20 | Zenon LISIECZKO | Plants and seeds of spring canola variety ND-662c |
| US8513494B2 (en) | 2011-04-08 | 2013-08-20 | Chunren Wu | Plants and seeds of spring canola variety SCV695971 |
| EP2794643A1 (en) | 2011-04-29 | 2014-10-29 | Pioneer Hi-Bred International Inc. | Down-regulation of a homeodomain-leucine zipper i-class homeobox gene for improved plant performance |
| US8507761B2 (en) | 2011-05-05 | 2013-08-13 | Teresa Huskowska | Plants and seeds of spring canola variety SCV372145 |
| US8513495B2 (en) | 2011-05-10 | 2013-08-20 | Dale Burns | Plants and seeds of spring canola variety SCV291489 |
| BR112013033176A2 (pt) | 2011-06-21 | 2018-06-12 | E I Du Pont De Nemouras And Company | método para produzir uma modificação direcionada em um gene de fertilidade masculina, planta, molécula de ácido nucleio isolada, construto de expressão |
| BR112014002622A2 (pt) | 2011-08-03 | 2019-09-24 | Du Pont | método para introduzir no genoma de uma célula vegetal um sítio alvo para a integração específica de sítio, célula vegetal, parte de planta, planta ou semente, método para integrar um polinucleotídeo de interesse em um sítio alvo no genoma de uma célula vegetal |
| AU2012301912A1 (en) | 2011-08-31 | 2014-03-06 | E. I. Dupont De Nemours & Company | Methods for tissue culture and transformation of sugarcane |
| US20140298544A1 (en) | 2011-10-28 | 2014-10-02 | Pioneer Hi Bred International Inc | Engineered PEP carboxylase variants for improved plant productivity |
| BR112014009954A2 (pt) | 2011-10-28 | 2017-12-05 | Du Pont | constructo, célula, planta, semente e método |
| CN104093842B (zh) | 2011-10-31 | 2016-12-07 | 先锋国际良种公司 | 改善植物耐旱性、氮利用效率和产量 |
| WO2013096818A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11268 |
| WO2013096810A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11482 |
| EP2800816A1 (en) | 2012-01-06 | 2014-11-12 | Pioneer Hi-Bred International Inc. | Ovule specific promoter and methods of use |
| WO2013103365A1 (en) | 2012-01-06 | 2013-07-11 | Pioneer Hi-Bred International, Inc. | Pollen preferred promoters and methods of use |
| AR089793A1 (es) | 2012-01-27 | 2014-09-17 | Du Pont | Metodos y composiciones para generar locus de rasgos transgenicos complejos |
| EA201491670A1 (ru) | 2012-03-13 | 2015-07-30 | Пайонир Хай-Бред Интернэшнл, Инк. | Генетическое снижение мужской репродуктивной функции у растений |
| WO2013138309A1 (en) | 2012-03-13 | 2013-09-19 | Pioneer Hi-Bred International, Inc. | Genetic reduction of male fertility in plants |
| US8859857B2 (en) | 2012-04-26 | 2014-10-14 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV259778 |
| US8878009B2 (en) | 2012-04-26 | 2014-11-04 | Monsanto Technology, LLP | Plants and seeds of spring canola variety SCV318181 |
| US8835720B2 (en) | 2012-04-26 | 2014-09-16 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV967592 |
| US8802935B2 (en) | 2012-04-26 | 2014-08-12 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV942568 |
| US9347105B2 (en) | 2012-06-15 | 2016-05-24 | Pioneer Hi Bred International Inc | Genetic loci associated with resistance of soybean to cyst nematode and methods of use |
| CA2876426A1 (en) | 2012-06-15 | 2013-12-19 | E. I. Du Pont De Nemours And Company | Methods and compositions involving als variants with native substrate preference |
| AU2012208997B1 (en) | 2012-07-30 | 2013-09-19 | Dlf Usa Inc. | An alfalfa variety named magnum salt |
| US9816102B2 (en) | 2012-09-13 | 2017-11-14 | Indiana University Research And Technology Corporation | Compositions and systems for conferring disease resistance in plants and methods of use thereof |
| WO2014059155A1 (en) | 2012-10-11 | 2014-04-17 | Pioneer Hi-Bred International, Inc. | Guard cell promoters and uses thereof |
| BR112015008504A2 (pt) | 2012-10-15 | 2017-08-22 | Pioneer Hi Bred Int | Método para aumentar a atividade pesticida, polipeptídeo, molécula de ácido nucleico, cassete de expressão, vetor, método de obtenção de uma planta, método de obtenção de uma célula de planta, composição pesticida, método para proteção de uma planta, método para proteger uma planta, método para aumentar a resistência de uma planta |
| US20150299718A1 (en) | 2012-11-20 | 2015-10-22 | Rajeev Gupta | Engineering Plants for Efficient Uptake and Utilization of Urea to Improve |
| US20140173781A1 (en) | 2012-12-13 | 2014-06-19 | Pioneer Hi-Bred International, Inc. | Methods and compositions for producing and selecting transgenic wheat plants |
| AU2013361220A1 (en) | 2012-12-21 | 2015-04-02 | Pioneer Hi-Bred International, Inc. | Compositions and methods for auxin-analog conjugation |
| US20150361447A1 (en) | 2013-01-25 | 2015-12-17 | Pioneer Hi-Breed International, Inc. | Maize event dp-032218-9 and methods for detection thereof |
| WO2014164014A1 (en) | 2013-03-11 | 2014-10-09 | Pioneer Hi-Bred International, Inc. | Genes for improving nutrient uptake and abiotic stress tolerance in plants |
| CN105473722A (zh) | 2013-03-11 | 2016-04-06 | 先锋国际良种公司 | 用于改善植物中化学信号扩散的方法及组合物 |
| WO2014164828A2 (en) | 2013-03-11 | 2014-10-09 | Pioneer Hi-Bred International, Inc. | Methods and compositions employing a sulfonylurea-dependent stabilization domain |
| WO2014164116A1 (en) | 2013-03-13 | 2014-10-09 | Pioneer Hi-Bred International, Inc. | Functional expression of bacterial major facilitator superfamily (sfm) gene in maize to improve agronomic traits and grain yield |
| WO2014164074A1 (en) | 2013-03-13 | 2014-10-09 | Pioneer Hi-Bred International, Inc. | Enhanced nitrate uptake and nitrate translocation by over-expressing maize functional low-affinity nitrate transporters in transgenic maize |
| CA2905743C (en) | 2013-03-13 | 2021-09-28 | Pioneer Hi-Bred International, Inc. | Glyphosate application for weed control in brassica |
| EP3744727A1 (en) | 2013-03-14 | 2020-12-02 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| CN105143248A (zh) | 2013-03-14 | 2015-12-09 | 先锋国际良种公司 | 玉蜀黍胁迫相关转录因子18及其用途 |
| US20160040149A1 (en) | 2013-03-14 | 2016-02-11 | Pioneer Hi-Bred International Inc. | Compositions Having Dicamba Decarboxylase Activity and Methods of Use |
| US20140289906A1 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Compositions Having Dicamba Decarboxylase Activity and Methods of Use |
| WO2014152507A2 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Modulation of acc deaminase expression |
| CA2903555A1 (en) | 2013-03-15 | 2014-09-18 | Pioneer Hi-Bred International, Inc. | Compositions and methods of use of acc oxidase polynucleotides and polypeptides |
| RU2015143825A (ru) | 2013-03-15 | 2017-04-26 | Пайонир Хай-Бред Интернэшнл, Инк. | Полипептиды phi-4 и способы их применения |
| US9797001B2 (en) | 2013-04-17 | 2017-10-24 | Pioneer Hi-Bred International, Inc. | Methods for characterizing a target DNA sequence composition in a plant genome |
| CN108823217B (zh) | 2013-08-08 | 2022-02-11 | 先锋国际良种公司 | 具有广谱活性的杀昆虫多肽及其用途 |
| EA030896B1 (ru) | 2013-08-16 | 2018-10-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| MX361154B (es) | 2013-08-22 | 2018-11-28 | Pioneer Hi Bred Int | Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso. |
| ES2937045T3 (es) | 2013-09-13 | 2023-03-23 | Pioneer Hi Bred Int | Proteínas insecticidas y métodos para su uso |
| BR112016008489A2 (pt) | 2013-10-18 | 2017-10-03 | Pioneer Hi Bred Int | Sequências de glifosato-n-acetiltransferase (glyat) e métodos de uso |
| UA120608C2 (uk) | 2014-02-07 | 2020-01-10 | Піонір Хай-Бред Інтернешнл, Інк. | Очищений поліпептид ptip-83 та спосіб його застосування |
| US9686931B2 (en) | 2014-07-07 | 2017-06-27 | Alforex Seeds LLC | Hybrid alfalfa variety named HybriForce-3400 |
| US10676754B2 (en) | 2014-07-11 | 2020-06-09 | E I Du Pont De Nemours And Company | Compositions and methods for producing plants resistant to glyphosate herbicide |
| US20170218384A1 (en) | 2014-08-08 | 2017-08-03 | Pioneer Hi-Bred International, Inc. | Ubiquitin promoters and introns and methods of use |
| RU2017112324A (ru) | 2014-09-12 | 2018-10-15 | Пайонир Хай-Бред Интернэшнл, Инк. | Создание сайтов сайт-специфической интеграции для сложных локусов признаков в кукурузе и сое, а также способы применения |
| US20170247719A1 (en) | 2014-09-17 | 2017-08-31 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| CA2962242A1 (en) | 2014-09-29 | 2016-04-07 | Agrigenetics, Inc. | Low lignin non-transgenic alfalfa varieties and methods for producing the same |
| WO2016061206A1 (en) | 2014-10-16 | 2016-04-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CN107074917B (zh) | 2014-10-16 | 2022-05-24 | 先锋国际良种公司 | 具有改进的活性谱的杀昆虫多肽及其用途 |
| CN106852153B (zh) | 2014-10-16 | 2023-06-06 | 先锋国际良种公司 | 具有广谱活性的杀昆虫多肽和其用途 |
| US10947556B2 (en) | 2014-12-19 | 2021-03-16 | AgBiome, Inc. | Sequences to facilitate incorporation of DNA into the genome of an organism |
| US20170359965A1 (en) | 2014-12-19 | 2017-12-21 | E I Du Pont De Nemours And Company | Polylactic acid compositions with accelerated degradation rate and increased heat stability |
| US11041158B2 (en) | 2014-12-22 | 2021-06-22 | AgBiome, Inc. | Optimization methods for making a synthetic gene |
| CA2985991A1 (en) | 2015-02-25 | 2016-09-01 | Andrew Mark CIGAN | Composition and methods for regulated expression of a guide rna/cas endonuclease complex |
| WO2016149352A1 (en) | 2015-03-19 | 2016-09-22 | Pioneer Hi-Bred International Inc | Methods and compositions for accelerated trait introgression |
| US20190136248A1 (en) | 2015-05-15 | 2019-05-09 | Pioneer Hi-Bred International, Inc. | Novel guide rna/cas endonuclease systems |
| BR112017024948A2 (pt) | 2015-05-19 | 2018-07-31 | Pioneer Hi Bred Int | proteínas inseticidas e métodos para uso das mesmas |
| MX2017016119A (es) | 2015-06-16 | 2018-03-07 | Pioneer Hi Bred Int | Composiciones y metodos para controlar plagas de insectos. |
| BR112018002535A2 (pt) | 2015-08-06 | 2018-09-25 | Du Pont | polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, planta transgênica ou célula de planta, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas ou uma praga e uso do polipeptídeo |
| AU2016315655A1 (en) | 2015-08-28 | 2018-02-01 | E. I. Du Pont De Nemours And Company | Ochrobactrum-mediated transformation of plants |
| EP4144844A1 (en) | 2015-10-12 | 2023-03-08 | DuPont US Holding, LLC | Protected dna templates for gene modification and increased homologous recombination in cells and methods of use |
| BR112018008109A2 (pt) | 2015-10-20 | 2018-11-06 | Pioneer Hi Bred Int | métodos para modificar uma sequência de nucleotídeos no genoma de uma célula vegetal, produzir uma planta, produzir tecido de calo de planta que tem uma sequência de nucleotídeos modificada em seu genoma sem o uso de um marcador selecionável, e planta de progênie da planta |
| AU2016350610A1 (en) | 2015-11-06 | 2018-04-12 | Pioneer Hi-Bred International, Inc. | Methods and compositions of improved plant transformation |
| US20180258438A1 (en) | 2015-11-06 | 2018-09-13 | Pioneer Hi-Bred International, Inc. | Generation of complex trait loci in soybean and methods of use |
| CN108575091A (zh) | 2015-12-18 | 2018-09-25 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| BR112018012887B1 (pt) | 2015-12-22 | 2024-02-06 | Pioneer Hi-Bred International, Inc | Cassete de expressão, vetor, métodos de obtenção de célula vegetal e planta transgênica, métodos para expressar um polinucleotídeo |
| US9896696B2 (en) | 2016-02-15 | 2018-02-20 | Benson Hill Biosystems, Inc. | Compositions and methods for modifying genomes |
| WO2017155715A1 (en) | 2016-03-11 | 2017-09-14 | Pioneer Hi-Bred International, Inc. | Novel cas9 systems and methods of use |
| US20190161742A1 (en) | 2016-03-11 | 2019-05-30 | Pioneer Hi-Bred International, Inc. | Novel cas9 systems and methods of use |
| CA3010628A1 (en) | 2016-03-11 | 2017-09-14 | Pioneer Hi-Bred International, Inc. | Novel cas9 systems and methods of use |
| BR112018070754A2 (pt) | 2016-04-14 | 2019-02-12 | Pioneer Hi-Bred International, Inc. | polipeptídeos, polinucleotídeos, construção de dna, célula hospedeira, plantas ou célula vegetal, semente, composição, método para controlar uma população de pragas lepidópteras, método para exterminar uma praga de lepidóptero, método para produzir um polipeptídeo com atividade pesticida e método para proteger uma planta contra uma praga de inseto |
| CN109072249B (zh) | 2016-04-19 | 2023-08-18 | 先锋国际良种公司 | 具有改善的活性谱的多肽的杀昆虫组合及其用途 |
| CN109068660B (zh) | 2016-05-04 | 2023-05-02 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| BR112018076027A2 (pt) | 2016-06-14 | 2019-03-26 | Pioneer Hi-Bred International, Inc. | método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo |
| EP3472323A1 (en) | 2016-06-16 | 2019-04-24 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| EP3472310A1 (en) | 2016-06-20 | 2019-04-24 | Pioneer Hi-Bred International, Inc. | Novel cas systems and methods of use |
| WO2017222821A2 (en) | 2016-06-24 | 2017-12-28 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
| WO2018005411A1 (en) | 2016-07-01 | 2018-01-04 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| WO2018013333A1 (en) | 2016-07-12 | 2018-01-18 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| WO2018060881A1 (en) | 2016-09-27 | 2018-04-05 | University Of Florida Research Foundation, Inc. | Insect toxin delivery mediated by a densovirus coat protein |
| CN116003539A (zh) | 2016-11-01 | 2023-04-25 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| WO2018140214A1 (en) | 2017-01-24 | 2018-08-02 | Pioneer Hi-Bred International, Inc. | Nematicidal protein from pseudomonas |
| US20200407737A1 (en) | 2017-05-03 | 2020-12-31 | KWS SAAT SE & Co. KGaA | Use of crispr-cas endonucleases for plant genome engineering |
| BR112019024827A2 (pt) | 2017-05-26 | 2020-06-16 | Pioneer Hi-Bred International, Inc. | Construto de dna, planta transgênica ou progênie da mesma, composição e método para controlar uma população de pragas de insetos |
| KR102631985B1 (ko) | 2017-08-09 | 2024-02-01 | 라이스텍, 인크. | 게놈을 변형시키기 위한 조성물 및 방법 |
| EP3688171A1 (en) | 2017-09-25 | 2020-08-05 | Pioneer Hi-Bred International, Inc. | Tissue-preferred promoters and methods of use |
| BR112020012477A2 (pt) | 2017-12-19 | 2020-11-24 | Pioneer Hi-Bred International, Inc. | polipeptídeo recombinante; polipeptídeo inseticida recombinante; composição agrícola; construto de dna; célula hospedeira; planta transgênica; método para inibir o crescimento ou exterminar uma praga de inseto ou população de praga; método para controlar a infestação de praga; e método para melhorar o rendimento de uma cultura |
| WO2019133371A1 (en) | 2017-12-27 | 2019-07-04 | Pioneer Hi-Bred International, Inc. | Transformation of dicot plants |
| WO2019157522A1 (en) | 2018-02-12 | 2019-08-15 | Curators Of The University Of Missouri | Small auxin upregulated (saur) gene for the improvement of plant root system architecture, waterlogging tolerance, drought resistance and yield |
| CA3087861A1 (en) | 2018-03-02 | 2019-09-06 | Pioneer Hi-Bred International, Inc. | Plant health assay |
| WO2019226508A1 (en) | 2018-05-22 | 2019-11-28 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
| WO2020005933A1 (en) | 2018-06-28 | 2020-01-02 | Pioneer Hi-Bred International, Inc. | Methods for selecting transformed plants |
| EP3874050A1 (en) | 2018-10-31 | 2021-09-08 | Pioneer Hi-Bred International, Inc. | Compositions and methods for ochrobactrum-mediated plant transformation |
| CN113412333A (zh) | 2019-03-11 | 2021-09-17 | 先锋国际良种公司 | 用于克隆植物生产的方法 |
| US20220154193A1 (en) | 2019-03-28 | 2022-05-19 | Pioneer Hi-Bred International, Inc. | Modified agrobacterium strains and use thereof for plant transformation |
| CA3138663A1 (en) | 2019-06-25 | 2020-12-30 | Inari Agriculture Technology, Inc. | Improved homology dependent repair genome editing |
| WO2021046526A1 (en) | 2019-09-05 | 2021-03-11 | Benson Hill, Inc. | Compositions and methods for modifying genomes |
| CA3186978A1 (en) | 2020-07-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| MX2023001641A (es) | 2020-08-10 | 2023-03-08 | Du Pont | Composiciones y metodos para mejorar la resistencia al tizon foliar norte?o en el maiz. |
| WO2022109289A1 (en) | 2020-11-20 | 2022-05-27 | AgBiome, Inc. | Compositions and methods for incorporation of dna into the genome of an organism |
| CA3198940A1 (en) | 2020-11-24 | 2022-06-02 | Rebekah Deter Kelly | Pesticidal genes and methods of use |
| US20240141311A1 (en) | 2021-04-22 | 2024-05-02 | North Carolina State University | Compositions and methods for generating male sterile plants |
| WO2022236060A1 (en) | 2021-05-06 | 2022-11-10 | AgBiome, Inc. | Pesticidal genes and methods of use |
| CA3239251A1 (en) | 2021-12-07 | 2023-06-15 | Rebekah Deter Kelly | Pesticidal genes and methods of use |
| WO2023119135A1 (en) | 2021-12-21 | 2023-06-29 | Benson Hill, Inc. | Compositions and methods for modifying genomes |
| WO2023141464A1 (en) | 2022-01-18 | 2023-07-27 | AgBiome, Inc. | Method for designing synthetic nucleotide sequences |
| WO2024044596A1 (en) | 2022-08-23 | 2024-02-29 | AgBiome, Inc. | Pesticidal genes and methods of use |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5521153A (en) * | 1987-10-02 | 1996-05-28 | Ciba-Geigy Corporation | Synergistic antifungal protein and compositions containing same |
| EP0318341B1 (en) * | 1987-10-20 | 1996-07-31 | Plant Genetic Systems, N.V. | A process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins in said plants |
| AU2802989A (en) * | 1987-11-02 | 1989-06-01 | Louisiana State University Agricultural And Mechanical College | Plants genetically enhanced for disease resistance |
| MX9200621A (es) * | 1991-02-14 | 1993-02-01 | Du Pont | Gen de una proteina con alto contenido de azufre de una semilla y metodo para aumentar el contenido de azufre en aminoacidos de las plantas. |
| CA2061862A1 (en) * | 1991-03-04 | 1992-09-05 | Jonathan Duvick | Natural and synthetic proteins with inhibitory activity towards pathogenic microorganisms |
| EP0598806A1 (en) * | 1991-08-09 | 1994-06-01 | E.I. Du Pont De Nemours And Company | Synthetic storage proteins with defined structure containing programmable levels of essential amino acids for improvement of the nutritional value of plants |
| DE69332974T2 (de) * | 1992-03-19 | 2004-05-19 | E.I. Du Pont De Nemours And Co., Wilmington | Nukleinsäurefragmente und verfahren zur steigerung des lysin- und threoningehaltes der pflanzensamen |
| AU5446694A (en) * | 1992-10-23 | 1994-05-24 | Pioneer Hi-Bred International, Inc. | Process for enhancing the content of a selected amino acid in a seed storage protein |
| AU6162294A (en) * | 1993-01-13 | 1994-08-15 | Pioneer Hi-Bred International, Inc. | High lysine derivatives of alpha-hordothionin |
| AU6355594A (en) * | 1993-03-02 | 1994-09-26 | E.I. Du Pont De Nemours And Company | Improved feedcrops enriched in sulfur amino acids and methods for improvement |
| US5703049A (en) * | 1996-02-29 | 1997-12-30 | Pioneer Hi-Bred Int'l, Inc. | High methionine derivatives of α-hordothionin for pathogen-control |
-
1996
- 1996-05-31 WO PCT/US1996/008220 patent/WO1996038563A1/en not_active Application Discontinuation
- 1996-05-31 AR ARP960102843A patent/AR004938A1/es unknown
- 1996-05-31 JP JP8536728A patent/JPH11506329A/ja active Pending
- 1996-05-31 PL PL96323635A patent/PL323635A1/xx unknown
- 1996-05-31 HU HU9900878A patent/HUP9900878A2/hu unknown
- 1996-05-31 CA CA002222600A patent/CA2222600A1/en not_active Abandoned
- 1996-05-31 BR BR9609299A patent/BR9609299A/pt not_active Application Discontinuation
- 1996-05-31 CN CN96195938A patent/CN1192239A/zh active Pending
- 1996-05-31 EP EP96916884A patent/EP0832235A1/en not_active Withdrawn
- 1996-05-31 AU AU59611/96A patent/AU707354B2/en not_active Ceased
- 1996-05-31 MX MX9709352A patent/MX9709352A/es unknown
-
1997
- 1997-03-26 US US08/824,382 patent/US5885802A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AR004938A1 (es) | 1999-04-07 |
| BR9609299A (pt) | 1999-05-11 |
| WO1996038563A1 (en) | 1996-12-05 |
| HUP9900878A2 (hu) | 1999-07-28 |
| MX9709352A (es) | 1998-02-28 |
| CA2222600A1 (en) | 1996-12-05 |
| PL323635A1 (en) | 1998-04-14 |
| US5885802A (en) | 1999-03-23 |
| AU5961196A (en) | 1996-12-18 |
| EP0832235A1 (en) | 1998-04-01 |
| AU707354B2 (en) | 1999-07-08 |
| JPH11506329A (ja) | 1999-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1192239A (zh) | α-hordothionin的高甲硫氨酸衍生物 | |
| CN1192238A (zh) | α-hordothionin的高苏氨酸衍生物 | |
| MXPA97009352A (en) | Alpha-hortodionine derivatives with high mettion content | |
| MXPA97009351A (en) | Alpha-hordotionine derivatives with high content treon | |
| AU661334B2 (en) | Synthetic storage proteins with defined structure containing programmable levels of essential amino acids for improvement of the nutritional value of plants | |
| CA2266316C (en) | Binary methods of increasing accumulation of essential amino acids in seeds | |
| CA2177351C (en) | Chimeric genes and methods for increasing the lysine content of the seeds of corn, soybean and rapeseed plants | |
| CA2280196C (en) | Chimeric genes and methods for increasing the lysine content of the seeds of plants | |
| US5703049A (en) | High methionine derivatives of α-hordothionin for pathogen-control | |
| US5773691A (en) | Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants | |
| BG98042A (bg) | Ген,кодиращ семенен протеин с високо съдържание на сяра и метод за повишаване на серни амино киселини в растенията | |
| CA2222673A1 (en) | Methods of increasing accumulation of essential amino acids in seeds | |
| CN1038465A (zh) | 在植物中诱导赖氨酸过量生成的方法 | |
| CA2919813C (en) | Gene capable of increasing seed protein content and method of use thereof | |
| Yu et al. | Seed-specific expression of the lysine-rich protein gene sb401 significantly increases both lysine and total protein content in maize seeds | |
| CN1334877A (zh) | 蛋白质的共表达 | |
| US20050005330A1 (en) | Chimeric genes and methods for increasing the lysine content of the seeds of plants | |
| CN101942450A (zh) | 强旱生植物霸王液泡膜钠氢逆向转运蛋白基因和植物表达载体及其植株遗传转化方法 | |
| US20200010843A1 (en) | Genetically Altered Alfalfa Producing Clovamide and/or Related Hydroxycinnamoyl Amides |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |