BR112018008109A2 - métodos para modificar uma sequência de nucleotídeos no genoma de uma célula vegetal, produzir uma planta, produzir tecido de calo de planta que tem uma sequência de nucleotídeos modificada em seu genoma sem o uso de um marcador selecionável, e planta de progênie da planta - Google Patents
métodos para modificar uma sequência de nucleotídeos no genoma de uma célula vegetal, produzir uma planta, produzir tecido de calo de planta que tem uma sequência de nucleotídeos modificada em seu genoma sem o uso de um marcador selecionável, e planta de progênie da plantaInfo
- Publication number
- BR112018008109A2 BR112018008109A2 BR112018008109A BR112018008109A BR112018008109A2 BR 112018008109 A2 BR112018008109 A2 BR 112018008109A2 BR 112018008109 A BR112018008109 A BR 112018008109A BR 112018008109 A BR112018008109 A BR 112018008109A BR 112018008109 A2 BR112018008109 A2 BR 112018008109A2
- Authority
- BR
- Brazil
- Prior art keywords
- plant
- genome
- nucleotide sequence
- producing
- methods
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
trata-se de composições e métodos para modificar uma sequência de nucleotídeos no genoma de uma célula vegetal sem o uso de um marcador selecionável. os métodos e as composições empregam um sistema de polinucleotídeo-guia/endonuclease cas para produzir uma ruptura de filamento duplo em um sítio-alvo localizado em uma sequência de nucleotídeos, e células vegetais são obtidas sem o uso de um marcador selecionável, e para fornecer um sistema eficaz para modificar sítios-alvo dentro do genoma de uma planta, célula vegetal ou semente. são também fornecidos composições e métodos para produzir uma célula vegetal, tecido de calo ou planta que tem uma sequência de nucleotídeos modificada em seu genoma, sem o uso de um marcador selecionável.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562243719P | 2015-10-20 | 2015-10-20 | |
US201662309033P | 2016-03-16 | 2016-03-16 | |
US201662359254P | 2016-07-07 | 2016-07-07 | |
PCT/US2016/057272 WO2017070029A1 (en) | 2015-10-20 | 2016-10-17 | Methods and compositions for marker-free genome modification |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112018008109A2 true BR112018008109A2 (pt) | 2018-11-06 |
Family
ID=57200160
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018008134A BR112018008134A2 (pt) | 2015-10-20 | 2016-10-17 | método para restaurar a função de um produto gênico não funcional no genoma de uma célula, método para editar uma sequência nucleotídica no genoma de uma célula, planta ou planta de progênie, método para editar uma sequência nucleotídica no genoma de uma célula sem o uso de um molde polinucleotídico de modificação e método para entregar um complexo de rna guia/endonuclease cas numa célula |
BR112018008109A BR112018008109A2 (pt) | 2015-10-20 | 2016-10-17 | métodos para modificar uma sequência de nucleotídeos no genoma de uma célula vegetal, produzir uma planta, produzir tecido de calo de planta que tem uma sequência de nucleotídeos modificada em seu genoma sem o uso de um marcador selecionável, e planta de progênie da planta |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018008134A BR112018008134A2 (pt) | 2015-10-20 | 2016-10-17 | método para restaurar a função de um produto gênico não funcional no genoma de uma célula, método para editar uma sequência nucleotídica no genoma de uma célula, planta ou planta de progênie, método para editar uma sequência nucleotídica no genoma de uma célula sem o uso de um molde polinucleotídico de modificação e método para entregar um complexo de rna guia/endonuclease cas numa célula |
Country Status (11)
Country | Link |
---|---|
US (3) | US20180282763A1 (pt) |
EP (2) | EP3365440B1 (pt) |
JP (2) | JP2018531024A (pt) |
KR (2) | KR20180059535A (pt) |
CN (2) | CN108138155A (pt) |
AU (2) | AU2016341044B2 (pt) |
BR (2) | BR112018008134A2 (pt) |
CA (2) | CA2996326A1 (pt) |
MX (2) | MX2018004808A (pt) |
WO (2) | WO2017070029A1 (pt) |
ZA (2) | ZA201801578B (pt) |
Families Citing this family (65)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
WO2015026887A1 (en) | 2013-08-22 | 2015-02-26 | E. I. Du Pont De Nemours And Company | A soybean u6 polymerase iii promoter and methods of use |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
US9576926B2 (en) | 2014-01-16 | 2017-02-21 | Taiwan Semiconductor Manufacturing Company, Ltd. | Pad structure design in fan-out package |
AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
PT3221457T (pt) | 2014-11-21 | 2019-06-27 | Regeneron Pharma | Métodos e composições para modificação genética visada através da utilização de arn guia emparelhados |
CN107567499A (zh) | 2015-03-27 | 2018-01-09 | 纳幕尔杜邦公司 | 大豆u6核小rna基因启动子及其在植物小rna基因的组成型表达中的用途 |
CN108513575A (zh) | 2015-10-23 | 2018-09-07 | 哈佛大学的校长及成员们 | 核碱基编辑器及其用途 |
WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
JP7127942B2 (ja) | 2016-12-22 | 2022-08-30 | キージーン ナムローゼ フェンノートシャップ | 二重鎖dnaの標的化改変の方法 |
US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
SG11201908658TA (en) | 2017-03-23 | 2019-10-30 | Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
CN110891965A (zh) | 2017-04-24 | 2020-03-17 | 杜邦营养生物科学有限公司 | 植物中使用的抗crispr蛋白的方法和组合物 |
KR20200004382A (ko) * | 2017-05-05 | 2020-01-13 | 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 | 전이유전자성 마커 서열을 이용하지 않는 세포 단리 방법 |
EP3622061A4 (en) * | 2017-05-11 | 2021-01-27 | Institute Of Genetics And Developmental Biology Chinese Academy of Sciences | CREATION OF A HERBICIDE RESISTANT GENE AND USE OF IT |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
CN111263810A (zh) | 2017-08-22 | 2020-06-09 | 纳匹基因公司 | 使用多核苷酸指导的核酸内切酶的细胞器基因组修饰 |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
CN111465321B (zh) * | 2017-10-13 | 2022-12-06 | 先锋国际良种公司 | 用于植物细胞的细胞重编程的系统和方法 |
CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
JP7384801B2 (ja) | 2017-12-15 | 2023-11-21 | ダニスコ・ユーエス・インク | Cas9変異体及び使用方法 |
US20210238614A1 (en) * | 2018-05-07 | 2021-08-05 | Pioneer Hi-Bred International, Inc. | Methods and compositions for homology directed repair of double strand breaks in plant cell genomes |
EP3794130A4 (en) | 2018-05-16 | 2022-07-27 | Synthego Corporation | METHODS AND SYSTEMS FOR DESIGN AND USE OF GUIDE RNA |
EP3575402A1 (en) * | 2018-06-01 | 2019-12-04 | Algentech SAS | Gene targeting |
GB201809273D0 (en) | 2018-06-06 | 2018-07-25 | Vib Vzw | Novel mutant plant cinnamoyl-coa reductase proteins |
US20210324398A1 (en) | 2018-06-29 | 2021-10-21 | Pioneer Hi-Bred International, Inc. | Edited nac genes in plants |
US20210277421A1 (en) * | 2018-08-06 | 2021-09-09 | NemaMetrix, Inc | Homologous Recombination Reporter Construct and Uses Thereof |
GB201820109D0 (en) | 2018-12-11 | 2019-01-23 | Vib Vzw | Plants with a lignin trait and udp-glycosyltransferase mutation |
EP3894550A4 (en) | 2018-12-14 | 2023-01-04 | Pioneer Hi-Bred International, Inc. | NEW CRISPR-CAS SYSTEMS FOR GENOME EDITING |
BR112021017998A2 (pt) | 2019-03-11 | 2021-11-16 | Pioneer Hi Bred Int | Métodos para a produção de plantas clonais |
DE112020001342T5 (de) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen |
US20220195455A1 (en) * | 2019-04-03 | 2022-06-23 | University Of Georgia Research Foundation, Inc. | Delivery of crispr/mcas9 through extracellular vesicles for genome editing |
WO2021003410A1 (en) * | 2019-07-03 | 2021-01-07 | Napigen, Inc. | Organelle genome modification |
WO2021067788A1 (en) | 2019-10-03 | 2021-04-08 | Artisan Development Labs, Inc. | Crispr systems with engineered dual guide nucleic acids |
US20210147862A1 (en) * | 2019-10-23 | 2021-05-20 | Pairwise Plants Services, Inc. | Compositions and methods for rna-templated editing in plants |
US20210121581A1 (en) * | 2019-10-29 | 2021-04-29 | Stitch Bio, Llc | Methods and compositions for treating tumor cells |
JP2023505600A (ja) | 2019-11-05 | 2023-02-09 | ペアーワイズ プランツ サービシズ, インコーポレイテッド | 対立遺伝子のrnaにコードされたdnaの置換のための組成物および方法 |
EP4110905A4 (en) * | 2020-02-24 | 2024-03-27 | Pioneer Hi-Bred International, Inc. | HOMOLOGOUS INTRAGENOMIC RECOMBINATION |
BR112022019960A2 (pt) * | 2020-03-30 | 2022-12-13 | Inari Agriculture Tech Inc | Método de modificação de um gene endógeno, método de modificação da expressão de um gene endógeno, célula vegetal, planta de soja, parte da planta, tecido ou tecido do calo, método para obter a célula vegetal, polinucleotídeo isolado, ácido nucleico recombinante |
EP4146804A1 (en) | 2020-05-08 | 2023-03-15 | The Broad Institute Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
KR20230010678A (ko) * | 2020-05-13 | 2023-01-19 | 넌헴스 비.브이. | 표적화된 돌연변이유발에 의해 돌연변이체 식물을 수득하는 방법 |
BR112022025102A2 (pt) * | 2020-06-09 | 2023-02-07 | Univ Georgia | Monocotiledôneas heterozigotas para cenh3 e métodos de uso das mesmas para indução haploide e edição simultânea de genoma |
JP2023545403A (ja) * | 2020-10-02 | 2023-10-30 | リマグレン、ヨーロッパ | Crisprを介した指令コドン書換え |
WO2022256448A2 (en) | 2021-06-01 | 2022-12-08 | Artisan Development Labs, Inc. | Compositions and methods for targeting, editing, or modifying genes |
EP4370676A2 (en) | 2021-06-18 | 2024-05-22 | Artisan Development Labs, Inc. | Compositions and methods for targeting, editing or modifying human genes |
WO2023102393A1 (en) | 2021-11-30 | 2023-06-08 | Pioneer Hi-Bred International, Inc. | High efficiency large scale chromosomal genome manipulation |
WO2023148291A1 (en) * | 2022-02-02 | 2023-08-10 | Biotalys NV | Methods for genome editing |
WO2023150773A2 (en) * | 2022-02-07 | 2023-08-10 | Inari Agriculture Technology, Inc. | Use of cas12f nucleases in production of expression modulated plant materials |
WO2023167882A1 (en) | 2022-03-01 | 2023-09-07 | Artisan Development Labs, Inc. | Composition and methods for transgene insertion |
WO2023192825A1 (en) * | 2022-03-29 | 2023-10-05 | Inari Agriculture Technology, Inc. | Targeted donor dna insertion and indel editing of plant genes |
WO2023225410A2 (en) | 2022-05-20 | 2023-11-23 | Artisan Development Labs, Inc. | Systems and methods for assessing risk of genome editing events |
WO2024036190A2 (en) | 2022-08-09 | 2024-02-15 | Pioneer Hi-Bred International, Inc. | Guide polynucleotide multiplexing |
WO2024033378A1 (en) * | 2022-08-09 | 2024-02-15 | Institute Of Molecular Biology Ggmbh (Imb) | Method of parallel, rapid and sensitive detection of dna double strand breaks |
WO2024123786A1 (en) | 2022-12-06 | 2024-06-13 | Pioneer Hi-Bred International, Inc. | Methods and compositions for co-delivery of t-dnas expressing multiple guide polynucleotides into plants |
Family Cites Families (101)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
US5304732A (en) | 1984-03-06 | 1994-04-19 | Mgi Pharma, Inc. | Herbicide resistance in plants |
US4761373A (en) | 1984-03-06 | 1988-08-02 | Molecular Genetics, Inc. | Herbicide resistance in plants |
US5331107A (en) | 1984-03-06 | 1994-07-19 | Mgi Pharma, Inc. | Herbicide resistance in plants |
US5036006A (en) * | 1984-11-13 | 1991-07-30 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
US5569597A (en) | 1985-05-13 | 1996-10-29 | Ciba Geigy Corp. | Methods of inserting viral DNA into plant material |
US5107065A (en) | 1986-03-28 | 1992-04-21 | Calgene, Inc. | Anti-sense regulation of gene expression in plant cells |
US5605011A (en) | 1986-08-26 | 1997-02-25 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5378824A (en) | 1986-08-26 | 1995-01-03 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5013659A (en) | 1987-07-27 | 1991-05-07 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
US5268463A (en) | 1986-11-11 | 1993-12-07 | Jefferson Richard A | Plant promoter α-glucuronidase gene construct |
US5608142A (en) | 1986-12-03 | 1997-03-04 | Agracetus, Inc. | Insecticidal cotton plants |
US4873192A (en) | 1987-02-17 | 1989-10-10 | The United States Of America As Represented By The Department Of Health And Human Services | Process for site specific mutagenesis without phenotypic selection |
US5316931A (en) | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
US5990387A (en) * | 1988-06-10 | 1999-11-23 | Pioneer Hi-Bred International, Inc. | Stable transformation of plant cells |
US5023179A (en) | 1988-11-14 | 1991-06-11 | Eric Lam | Promoter enhancer element for gene expression in plant roots |
US5110732A (en) | 1989-03-14 | 1992-05-05 | The Rockefeller University | Selective gene expression in plants |
US5231020A (en) | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
US5034323A (en) | 1989-03-30 | 1991-07-23 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
US5879918A (en) | 1989-05-12 | 1999-03-09 | Pioneer Hi-Bred International, Inc. | Pretreatment of microprojectiles prior to using in a particle gun |
US5240855A (en) | 1989-05-12 | 1993-08-31 | Pioneer Hi-Bred International, Inc. | Particle gun |
US5310667A (en) | 1989-07-17 | 1994-05-10 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases |
US5322783A (en) | 1989-10-17 | 1994-06-21 | Pioneer Hi-Bred International, Inc. | Soybean transformation by microparticle bombardment |
EP0452269B1 (en) | 1990-04-12 | 2002-10-09 | Syngenta Participations AG | Tissue-preferential promoters |
CA2079877C (en) | 1990-04-26 | 2001-07-31 | Marnix Peferoen | Bacillus thuringiensis strains and their genes encoding insecticidal toxins |
US5478369A (en) | 1990-06-12 | 1995-12-26 | Pioneer Hi-Bred International, Inc. | Nucleotide sequences mediating male fertility and method of using same |
US5498830A (en) | 1990-06-18 | 1996-03-12 | Monsanto Company | Decreased oil content in plant seeds |
US5633435A (en) | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
US5866775A (en) | 1990-09-28 | 1999-02-02 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases |
US5932782A (en) | 1990-11-14 | 1999-08-03 | Pioneer Hi-Bred International, Inc. | Plant transformation method using agrobacterium species adhered to microprojectiles |
US5277905A (en) | 1991-01-16 | 1994-01-11 | Mycogen Corporation | Coleopteran-active bacillus thuringiensis isolate |
US5459252A (en) | 1991-01-31 | 1995-10-17 | North Carolina State University | Root specific gene promoter |
US5399680A (en) | 1991-05-22 | 1995-03-21 | The Salk Institute For Biological Studies | Rice chitinase promoter |
GB9114259D0 (en) | 1991-07-02 | 1991-08-21 | Ici Plc | Plant derived enzyme and dna sequences |
US5731180A (en) | 1991-07-31 | 1998-03-24 | American Cyanamid Company | Imidazolinone resistant AHAS mutants |
ES2124738T3 (es) | 1991-08-02 | 1999-02-16 | Mycogen Corp | Nuevo microorganismo e insecticida. |
HU219057B (hu) | 1991-08-27 | 2001-02-28 | Novartis Ag. | Inszekticid hatású proteinek Homoptera rovarok ellen és alkalmazásuk a növényvédelemben |
JPH06511152A (ja) | 1991-10-04 | 1994-12-15 | ノースカロライナ ステイト ユニバーシティー | 病原体耐性トランスジェニック植物 |
TW261517B (pt) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
US5324646A (en) | 1992-01-06 | 1994-06-28 | Pioneer Hi-Bred International, Inc. | Methods of regeneration of Medicago sativa and expressing foreign DNA in same |
US5401836A (en) | 1992-07-16 | 1995-03-28 | Pioneer Hi-Bre International, Inc. | Brassica regulatory sequence for root-specific or root-abundant gene expression |
EP0652965A1 (en) | 1992-07-27 | 1995-05-17 | Pioneer Hi-Bred International, Inc. | An improved method of agrobacterium-mediated transformation of cultured soybean cells |
IL108241A (en) | 1992-12-30 | 2000-08-13 | Biosource Genetics Corp | Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus |
DE69428290T2 (de) | 1993-01-13 | 2002-04-18 | Pioneer Hi Bred Int | Derivate von alpha-hordothionin mit höherem behalt an lysin |
US5789156A (en) | 1993-06-14 | 1998-08-04 | Basf Ag | Tetracycline-regulated transcriptional inhibitors |
US5814618A (en) | 1993-06-14 | 1998-09-29 | Basf Aktiengesellschaft | Methods for regulating gene expression |
US5593881A (en) | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
US5633363A (en) | 1994-06-03 | 1997-05-27 | Iowa State University, Research Foundation In | Root preferential promoter |
US5736369A (en) | 1994-07-29 | 1998-04-07 | Pioneer Hi-Bred International, Inc. | Method for producing transgenic cereal plants |
US5608144A (en) | 1994-08-12 | 1997-03-04 | Dna Plant Technology Corp. | Plant group 2 promoters and uses thereof |
US5792931A (en) | 1994-08-12 | 1998-08-11 | Pioneer Hi-Bred International, Inc. | Fumonisin detoxification compositions and methods |
EP0711834A3 (en) | 1994-10-14 | 1996-12-18 | Nissan Chemical Ind Ltd | New Bacillus strain and product for the control of harmful organisms |
US5659026A (en) | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
US5853973A (en) | 1995-04-20 | 1998-12-29 | American Cyanamid Company | Structure based designed herbicide resistant products |
BR9604993B1 (pt) | 1995-04-20 | 2009-05-05 | dna mutante codificando uma proteìna ahas mutante de sìntese de ácido acetohidróxi e proteìnas ahas mutantes. | |
AU705933B2 (en) | 1995-06-02 | 1999-06-03 | Pioneer Hi-Bred International, Inc. | High threonine derivatives of alpha-hordothionin |
EP0832235A1 (en) | 1995-06-02 | 1998-04-01 | Pioneer Hi-Bred International, Inc. | HIGH METHIONINE DERIVATIVES OF alfa-HORDOTHIONIN |
US5837876A (en) | 1995-07-28 | 1998-11-17 | North Carolina State University | Root cortex specific gene promoter |
US5703049A (en) | 1996-02-29 | 1997-12-30 | Pioneer Hi-Bred Int'l, Inc. | High methionine derivatives of α-hordothionin for pathogen-control |
ATE229977T1 (de) | 1996-03-26 | 2003-01-15 | Razvan T Radulescu | Peptide mit antiproliferativen eigenschaften |
US6072050A (en) | 1996-06-11 | 2000-06-06 | Pioneer Hi-Bred International, Inc. | Synthetic promoters |
AU3495297A (en) | 1996-07-08 | 1998-02-02 | Pioneer Hi-Bred International, Inc. | Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs |
US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
AU762993C (en) | 1998-02-26 | 2004-06-10 | Pioneer Hi-Bred International, Inc. | Constitutive maize promoters |
DE69928264T2 (de) | 1998-08-20 | 2006-07-13 | Pioneer Hi-Bred International, Inc. | Samen-bevorzugende promotoren |
WO2000012733A1 (en) | 1998-08-28 | 2000-03-09 | Pioneer Hi-Bred International, Inc. | Seed-preferred promoters from end genes |
CN1360634A (zh) | 1999-04-29 | 2002-07-24 | 辛甄塔有限公司 | 抗除草剂植物 |
US7612251B2 (en) | 2000-09-26 | 2009-11-03 | Pioneer Hi-Bred International, Inc. | Nucleotide sequences mediating male fertility and method of using same |
US7517975B2 (en) | 2000-09-26 | 2009-04-14 | Pioneer Hi-Bred International, Inc. | Nucleotide sequences mediating male fertility and method of using same |
BR0114199A (pt) | 2000-09-26 | 2006-01-31 | Pioneer Hi Bred Int | Sequências de nucleotìdeos que medeiam a fertilidade masculina e métodos de uso das mesmas |
JP2004528808A (ja) | 2000-09-29 | 2004-09-24 | シンジェンタ リミテッド | 除草剤抵抗性植物 |
SI1495330T1 (sl) | 2002-04-12 | 2009-06-30 | Dowd Brian F O | Postopki identificiranja spojin, ki medsebojno vplivajo na transmembranske proteine |
US7045684B1 (en) | 2002-08-19 | 2006-05-16 | Mertec, Llc | Glyphosate-resistant plants |
AU2005210493B2 (en) | 2004-02-02 | 2009-06-04 | Pioneer Hi-Bred International, Inc. | AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use |
US20070016985A1 (en) | 2005-07-18 | 2007-01-18 | Pioneer Hi-Bred International, Inc. | Particle Preparation for Direct-Delivery Transformation |
EP2325332B1 (en) | 2005-08-26 | 2012-10-31 | DuPont Nutrition Biosciences ApS | Use of CRISPR associated genes (CAS) |
RU2441366C2 (ru) | 2006-01-12 | 2012-02-10 | Сайбас Юроп Б.В. | Мутанты epsps |
RU2485180C2 (ru) | 2007-06-07 | 2013-06-20 | Эгрикалча Энд Эгри-Фуд Кэнэда | Способ трансфекции и трансдукции растительных клеток |
US7919676B2 (en) | 2007-08-03 | 2011-04-05 | Pioneer Hi-Bred International, Inc. | Msca1 nucleotide sequences impacting plant male fertility and method of using same |
UA106458C2 (uk) | 2008-10-28 | 2014-08-26 | Піонер Хай-Бред Інтернешенл, Інк. | Чутливий до сульфонілсечовини репресорний білок |
EP2529018B1 (en) | 2009-12-30 | 2016-06-22 | Pioneer Hi-Bred International, Inc. | Methods and compositions for the introduction and regulated expression of genes in plants |
US10030245B2 (en) | 2011-03-23 | 2018-07-24 | E I Du Pont De Nemours And Company | Methods for producing a complex transgenic trait locus |
WO2013088446A1 (en) | 2011-12-16 | 2013-06-20 | Targetgene Biotechnologies Ltd | Compositions and methods for modifying a predetermined target nucleic acid sequence |
AR089793A1 (es) | 2012-01-27 | 2014-09-17 | Du Pont | Metodos y composiciones para generar locus de rasgos transgenicos complejos |
WO2013119888A1 (en) | 2012-02-09 | 2013-08-15 | President And Fellows Of Harvard College | Selective nucleic acid amplification from nucleic acid pools |
US10723806B2 (en) | 2012-02-27 | 2020-07-28 | Riken | Method of introducing nucleic acid into plant cells |
BR112014028615A2 (pt) * | 2012-05-18 | 2017-06-27 | Pioneer Hi Bred Int | polinucleotídeos, constructo de dna, vetor, célula, plantas, semente, método de expressão, métodos para alterar a expressão e para alterar uma característica de planta. |
DK3401400T3 (da) | 2012-05-25 | 2019-06-03 | Univ California | Fremgangsmåder og sammensætninger til rna-styret mål-dna-modifikation og til rna-styret transskriptionsmodulering |
WO2014036219A2 (en) * | 2012-08-29 | 2014-03-06 | Sangamo Biosciences, Inc. | Methods and compositions for treatment of a genetic condition |
CN110643600A (zh) * | 2012-10-23 | 2020-01-03 | 基因工具股份有限公司 | 用于切割靶dna的系统及其用途 |
CN105658796B (zh) | 2012-12-12 | 2021-10-26 | 布罗德研究所有限公司 | 用于序列操纵的crispr-cas组分系统、方法以及组合物 |
US20140273230A1 (en) * | 2013-03-15 | 2014-09-18 | Sigma-Aldrich Co., Llc | Crispr-based genome modification and regulation |
JP2016521561A (ja) | 2013-06-14 | 2016-07-25 | セレクティス | 植物における非トランスジェニックのゲノム編集のための方法 |
US20150044772A1 (en) * | 2013-08-09 | 2015-02-12 | Sage Labs, Inc. | Crispr/cas system-based novel fusion protein and its applications in genome editing |
WO2015026887A1 (en) | 2013-08-22 | 2015-02-26 | E. I. Du Pont De Nemours And Company | A soybean u6 polymerase iii promoter and methods of use |
CN103667338B (zh) * | 2013-11-28 | 2016-01-27 | 中国科学院遗传与发育生物学研究所 | 一种玉米基因组定点改造方法 |
CN104805078A (zh) * | 2014-01-28 | 2015-07-29 | 北京大学 | 用于高效基因组编辑的rna分子的设计、合成及其应用 |
CN106232803A (zh) | 2014-02-27 | 2016-12-14 | 孟山都技术公司 | 用于定点基因组修饰的组合物和方法 |
WO2015171894A1 (en) | 2014-05-09 | 2015-11-12 | The Regents Of The University Of California | Methods for selecting plants after genome editing |
EA038896B1 (ru) | 2015-03-16 | 2021-11-03 | Институт Генетики И Биологии Развития Академии Наук Китая | Способ осуществления сайт-направленной модификации растительных геномов с использованием ненаследуемых материалов |
-
2016
- 2016-10-17 MX MX2018004808A patent/MX2018004808A/es unknown
- 2016-10-17 EP EP16788879.1A patent/EP3365440B1/en active Active
- 2016-10-17 US US15/765,039 patent/US20180282763A1/en not_active Abandoned
- 2016-10-17 KR KR1020187012258A patent/KR20180059535A/ko unknown
- 2016-10-17 EP EP16785676.4A patent/EP3365439A1/en not_active Withdrawn
- 2016-10-17 US US15/763,758 patent/US20180273960A1/en active Pending
- 2016-10-17 WO PCT/US2016/057272 patent/WO2017070029A1/en active Application Filing
- 2016-10-17 CN CN201680059024.1A patent/CN108138155A/zh active Pending
- 2016-10-17 CN CN201680060783.XA patent/CN108138156A/zh active Pending
- 2016-10-17 WO PCT/US2016/057279 patent/WO2017070032A1/en active Application Filing
- 2016-10-17 BR BR112018008134A patent/BR112018008134A2/pt unknown
- 2016-10-17 KR KR1020187012259A patent/KR20180069832A/ko active Search and Examination
- 2016-10-17 AU AU2016341044A patent/AU2016341044B2/en active Active
- 2016-10-17 MX MX2018004809A patent/MX2018004809A/es unknown
- 2016-10-17 BR BR112018008109A patent/BR112018008109A2/pt not_active Application Discontinuation
- 2016-10-17 CA CA2996326A patent/CA2996326A1/en not_active Abandoned
- 2016-10-17 AU AU2016341041A patent/AU2016341041A1/en not_active Abandoned
- 2016-10-17 CA CA2996329A patent/CA2996329A1/en active Pending
- 2016-10-17 JP JP2018520177A patent/JP2018531024A/ja not_active Withdrawn
-
2018
- 2018-03-07 ZA ZA2018/01578A patent/ZA201801578B/en unknown
- 2018-03-07 ZA ZA2018/01576A patent/ZA201801576B/en unknown
- 2018-07-24 US US16/044,019 patent/US20180327785A1/en active Pending
-
2021
- 2021-07-02 JP JP2021110756A patent/JP2021151275A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2017070029A1 (en) | 2017-04-27 |
US20180273960A1 (en) | 2018-09-27 |
KR20180069832A (ko) | 2018-06-25 |
ZA201801578B (en) | 2018-11-28 |
CA2996329A1 (en) | 2017-04-27 |
CN108138155A (zh) | 2018-06-08 |
KR20180059535A (ko) | 2018-06-04 |
AU2016341041A1 (en) | 2018-03-15 |
AU2016341044A1 (en) | 2018-03-15 |
EP3365439A1 (en) | 2018-08-29 |
MX2018004809A (es) | 2018-06-08 |
AU2016341044B2 (en) | 2023-03-09 |
WO2017070032A1 (en) | 2017-04-27 |
EP3365440B1 (en) | 2022-09-14 |
EP3365440A1 (en) | 2018-08-29 |
ZA201801576B (en) | 2018-11-28 |
BR112018008134A2 (pt) | 2018-11-06 |
CA2996326A1 (en) | 2017-04-27 |
CN108138156A (zh) | 2018-06-08 |
US20180282763A1 (en) | 2018-10-04 |
JP2021151275A (ja) | 2021-09-30 |
MX2018004808A (es) | 2018-08-01 |
US20180327785A1 (en) | 2018-11-15 |
JP2018531024A (ja) | 2018-10-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR112018008109A2 (pt) | métodos para modificar uma sequência de nucleotídeos no genoma de uma célula vegetal, produzir uma planta, produzir tecido de calo de planta que tem uma sequência de nucleotídeos modificada em seu genoma sem o uso de um marcador selecionável, e planta de progênie da planta | |
BR112017018327A2 (pt) | método para a expressão regulada de um complexo, planta ou célula vegetal, semente, método para modificação de uma sequência, método para alteração da expressão de pelo menos um polinucleotídeo | |
BR112016003561A2 (pt) | métodos para produzir modificações genéticas em um genoma de planta sem a incorporação de um transgene marcador selecionável, e composições dos mesmos | |
BR112018004108A2 (pt) | ochrobactrum haywardense h1 isolada, ochrobactrum haywardense h1, método para produzir uma célula vegetal transformada, kit e vetor | |
BR112018076027A2 (pt) | método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo | |
DOP2017000061A (es) | Composiciones microbianas y métodos | |
BR112017003757A2 (pt) | ?plantas de milho, partes de planta de milho ou sementes de milho? | |
BR112018007351A2 (pt) | métodos de seleção de células e levedura | |
BR112017012837A2 (pt) | sistemas de modificação de genoma fúngico e métodos de uso | |
BR112016013201A2 (pt) | administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para edição de genoma | |
BR112017000621A2 (pt) | método para melhorar um traço agronômico de uma planta, método para melhorar a produtividade de grãos ou a eficiência de uso de nitrogênio de uma planta de milho ou de cultura, método para editar, substituir ou eliminar uma sequência reguladora, método para inserir um promotor ou elemento do promotor no genoma de uma célula vegetal e método para fornecer um perfil de expressão adicional para um polinucleotídeo endógeno de uma célula vegetal | |
AR101277A1 (es) | Composiciones de lipo-quitooligosacáridos para mejorar el crecimiento de las plantas | |
CL2017003456A1 (es) | Metodos para tratar tumores de celulas epitelioides | |
BR112017015368A2 (pt) | método para a modificação precisa da planta através da expressão transiente do gene. | |
BR112017005044A2 (pt) | composições compreendendo uma ou mais cepas microbianas, célula vegetal tendo um revestimento, método para produzir um consórcio microbiano, consórcio microbiano, método para melhorar a saúde, o crescimento ou a produtividade de uma planta | |
BR112017016431A2 (pt) | método para realização de modificação direcionada de sítio para genomas de plantas através de uso de materiais não herdados | |
EP3313991A4 (en) | Modified factor ix, and compositions, methods and uses for gene transfer to cells, organs and tissues | |
BR112017017279A2 (pt) | métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta | |
WO2016073990A3 (en) | Methods for improving crispr/cas-mediated genome-editing | |
BR112017014230A2 (pt) | endófitos de semente através de cultivares e espécies, composições associadas e métodos de uso dos mesmos | |
BR112016013520A2 (pt) | Composições e métodos de uso de sistemas crispr-cas em distúrbios de repetições de nucleotídeos | |
BR112014027227A2 (pt) | modificação alvejada de malato desidrogenase | |
MX2022006948A (es) | Composiciones y metodos para programar celulas terapeuticas utilizando nanoportadores de acidos nucleicos dirigidos. | |
EP3471733A4 (en) | REAGENTS, COMPOSITIONS AND METHODS FOR IMPROVING THE VIABILITY AND FUNCTIONALITY OF CELLS, TISSUES AND ORGANS | |
BR112019007327A2 (pt) | método para obter uma célula vegetal, célula vegetal, planta, semente, polinucleotídeo-guia |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |