BR112017016431A2 - método para realização de modificação direcionada de sítio para genomas de plantas através de uso de materiais não herdados - Google Patents

método para realização de modificação direcionada de sítio para genomas de plantas através de uso de materiais não herdados

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Publication number
BR112017016431A2
BR112017016431A2 BR112017016431A BR112017016431A BR112017016431A2 BR 112017016431 A2 BR112017016431 A2 BR 112017016431A2 BR 112017016431 A BR112017016431 A BR 112017016431A BR 112017016431 A BR112017016431 A BR 112017016431A BR 112017016431 A2 BR112017016431 A2 BR 112017016431A2
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Brazil
Prior art keywords
nuclease
plant
target
site directed
inherited
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BR112017016431A
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English (en)
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BR112017016431B1 (pt
Inventor
Gao Caixia
Song Qianna
Shan Qiwei
Wang Yanpeng
Liang Zhen
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Inst Genetics & Developmental Biology Cas
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Application filed by Inst Genetics & Developmental Biology Cas filed Critical Inst Genetics & Developmental Biology Cas
Publication of BR112017016431A2 publication Critical patent/BR112017016431A2/pt
Publication of BR112017016431B1 publication Critical patent/BR112017016431B1/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se a um método de aplicação de uma substância não genética para realizar reforma direcionada de sítio para um genoma de planta. o método compreende as seguintes etapas: introduzir uma substância não genética em uma célula ou um tecido ou uma parte da planta de uma planta alvo; a sustância não genética sendo uma nuclease específica para o segmento alvo ou um mrna expressando a nuclease, a nuclease sendo uma talen nuclease, uma nucleasse de dedo de zinco (zfn), uma nuclease de repetições palindrômicas curtas agrupadas e regularmente interespaçadas (crispr/cas9), ou todas as nucleases capazes de realizar edição de genoma. a nuclease atua para cortar o segmento alvo, e então, a reforma direcionada de sítio do segmento alvo é completada através de reparo de dna da planta.
BR112017016431-0A 2015-03-16 2016-03-14 Método para conduzir modificação sítio-direcionada a fragmento alvo de gene alvo em planta e método para fabricar planta mutante livre de transgene BR112017016431B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201510114017.4 2015-03-16
CN201510114017 2015-03-16
PCT/CN2016/076244 WO2016155482A1 (zh) 2015-03-16 2016-03-14 一种利用非遗传物质对植物基因组进行定点改造的方法

Publications (2)

Publication Number Publication Date
BR112017016431A2 true BR112017016431A2 (pt) 2018-04-10
BR112017016431B1 BR112017016431B1 (pt) 2024-08-27

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Also Published As

Publication number Publication date
US20180163232A1 (en) 2018-06-14
AU2016239037A1 (en) 2017-08-10
AU2016239037B2 (en) 2022-04-21
WO2016155482A1 (zh) 2016-10-06
EP3279321A4 (en) 2018-10-31
US12043835B2 (en) 2024-07-23
CN105985943A (zh) 2016-10-05
KR20170126502A (ko) 2017-11-17
KR102194612B1 (ko) 2020-12-23
AR103926A1 (es) 2017-06-14
JP2023018066A (ja) 2023-02-07
UA125246C2 (uk) 2022-02-09
EA038896B1 (ru) 2021-11-03
EP3279321A1 (en) 2018-02-07
CA2979290A1 (en) 2016-10-06
JP2018508221A (ja) 2018-03-29
EA201792036A1 (ru) 2018-06-29
CN105985943B (zh) 2021-07-06

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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