BR112022025102A2 - Monocotiledôneas heterozigotas para cenh3 e métodos de uso das mesmas para indução haploide e edição simultânea de genoma - Google Patents

Monocotiledôneas heterozigotas para cenh3 e métodos de uso das mesmas para indução haploide e edição simultânea de genoma

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Publication number
BR112022025102A2
BR112022025102A2 BR112022025102A BR112022025102A BR112022025102A2 BR 112022025102 A2 BR112022025102 A2 BR 112022025102A2 BR 112022025102 A BR112022025102 A BR 112022025102A BR 112022025102 A BR112022025102 A BR 112022025102A BR 112022025102 A2 BR112022025102 A2 BR 112022025102A2
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BR
Brazil
Prior art keywords
cenh3
monocot
haploid
allele
methods
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Application number
BR112022025102A
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English (en)
Inventor
Kelly Dawe R
Jackson David
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Univ Georgia
Cold Spring Harbor Laboratory
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Application filed by Univ Georgia, Cold Spring Harbor Laboratory filed Critical Univ Georgia
Publication of BR112022025102A2 publication Critical patent/BR112022025102A2/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
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    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Life Sciences & Earth Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract

MONOCOTILEDÔNEAS HETEROZIGOTAS PARA CENH3 E MÉTODOS DE USO DAS MESMAS PARA INDUÇÃO HAPLOIDE E EDIÇÃO SIMULTÂNEA DE GENOMA. Trata-se de plantas monocotiledôneas heterozigotas para histona 3 centromérica (CenH3) e, opcionalmente, que expressam construtos de edição de genes para uso na indução de haploides de uma planta alvo monocotiledônea e, opcionalmente, edição de genes de passagem. As plantas indutoras haploides monocotiledôneas são tipicamente compostas por células vegetais diploides com apenas um alelo que codifica uma proteína CENH3 funcional. As células vegetais diploides também podem incluir, por exemplo, um alelo CenH3 que codifica a proteína CENH3 não funcional. Em algumas modalidades, o alelo que codifica proteína CENH3 não funcional é uma mutação por mudança da matriz de leitura, alelo nulo de proteína, um alelo nulo de RNA ou uma combinação dos mesmos. A planta indutora haploide monocotiledônea também pode incluir um maquinário de edição de genes, tal como uma nuclease sítio-dirigida e, opcionalmente, um RNA guia expresso de maneira estável por células da planta monocotiledônea. Também são fornecidos métodos para induzir a formação de uma planta monocotiledônea haploide alvo enquanto, opcionalmente, modifica de forma simultânea o genoma da planta monocotiledônea alvo.
BR112022025102A 2020-06-09 2021-06-09 Monocotiledôneas heterozigotas para cenh3 e métodos de uso das mesmas para indução haploide e edição simultânea de genoma BR112022025102A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063036910P 2020-06-09 2020-06-09
US202063036902P 2020-06-09 2020-06-09
PCT/US2021/036605 WO2021252619A1 (en) 2020-06-09 2021-06-09 Heterozygous cenh3 monocots and methods of use thereof for haploid induction and simultaneous genome editing

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BR112022025102A2 true BR112022025102A2 (pt) 2023-02-07

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US (1) US20230270067A1 (pt)
EP (1) EP4161253A1 (pt)
KR (1) KR20230021743A (pt)
CN (1) CN115811937A (pt)
AU (1) AU2021286555A1 (pt)
BR (1) BR112022025102A2 (pt)
CA (1) CA3181878A1 (pt)
CL (2) CL2022003484A1 (pt)
CO (1) CO2022017885A2 (pt)
CR (1) CR20220673A (pt)
IL (1) IL298774A (pt)
MX (1) MX2022015615A (pt)
WO (1) WO2021252619A1 (pt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024036240A1 (en) * 2022-08-11 2024-02-15 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
CN116463348B (zh) * 2023-05-26 2024-05-14 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统编辑玉米ZmCENH3基因的sg RNA及其应用
CN117305326B (zh) * 2023-11-29 2024-02-09 中国农业科学院蔬菜花卉研究所 青花菜BoCENH3基因及其在单倍体诱导中的应用

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WO2009137135A2 (en) * 2008-02-15 2009-11-12 Zgi, Inc. On-orbit procedures for adapting plants and animals to hostile environments
US8618354B2 (en) * 2009-10-06 2013-12-31 The Regents Of The University Of California Generation of haploid plants and improved plant breeding
US20120266324A1 (en) * 2011-04-15 2012-10-18 Pioneer Hi Bred International Inc. Self-Reproducing Hybrid Plants
US9526243B2 (en) * 2012-08-06 2016-12-27 E I Du Pont De Nemours And Company Pollination success and seed set in corn hybrid seed production
PL3186381T3 (pl) * 2014-08-28 2022-10-31 KWS SAAT SE & Co. KGaA Wytwarzanie roślin haploidalnych
CA2996329A1 (en) * 2015-10-20 2017-04-27 Pioneer Hi-Bred International, Inc. Restoring function to a non-functional gene product via guided cas systems and methods of use
NL2015753B1 (en) * 2015-11-09 2017-05-26 Rijk Zwaan Zaadteelt En Zaadhandel Bv Non-transgenic haploid inducer lines in cucurbits.
EP3457837A1 (en) * 2016-05-20 2019-03-27 Keygene N.V. Method for the production of haploid and subsequent doubled haploid plants
CN109982560A (zh) * 2016-12-02 2019-07-05 先正达参股股份有限公司 同时基因编辑和单倍体诱导
US10519456B2 (en) * 2016-12-02 2019-12-31 Syngenta Participations Ag Simultaneous gene editing and haploid induction
CN109943585B (zh) * 2018-04-12 2020-05-15 中国水稻研究所 一种利用植物杂种优势的方法
WO2019234129A1 (en) * 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Haploid induction with modified dna-repair
CA3112005A1 (en) * 2018-10-12 2020-04-16 Syngenta Crop Protection Ag Novel wheat cenh3 alleles

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CL2024000004A1 (es) 2024-05-31
EP4161253A1 (en) 2023-04-12
KR20230021743A (ko) 2023-02-14
AU2021286555A1 (en) 2023-02-02
CO2022017885A2 (es) 2023-02-06
CR20220673A (es) 2023-05-25
IL298774A (en) 2023-02-01
US20230270067A1 (en) 2023-08-31
CA3181878A1 (en) 2021-12-16
CN115811937A (zh) 2023-03-17
CL2022003484A1 (es) 2023-07-21
MX2022015615A (es) 2023-03-08
WO2021252619A1 (en) 2021-12-16

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