BR112022025102A2 - HETEROZYGOTES MONOCOT FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING - Google Patents
HETEROZYGOTES MONOCOT FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITINGInfo
- Publication number
- BR112022025102A2 BR112022025102A2 BR112022025102A BR112022025102A BR112022025102A2 BR 112022025102 A2 BR112022025102 A2 BR 112022025102A2 BR 112022025102 A BR112022025102 A BR 112022025102A BR 112022025102 A BR112022025102 A BR 112022025102A BR 112022025102 A2 BR112022025102 A2 BR 112022025102A2
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- Brazil
- Prior art keywords
- cenh3
- monocot
- haploid
- allele
- methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Physiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
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Abstract
MONOCOTILEDÔNEAS HETEROZIGOTAS PARA CENH3 E MÉTODOS DE USO DAS MESMAS PARA INDUÇÃO HAPLOIDE E EDIÇÃO SIMULTÂNEA DE GENOMA. Trata-se de plantas monocotiledôneas heterozigotas para histona 3 centromérica (CenH3) e, opcionalmente, que expressam construtos de edição de genes para uso na indução de haploides de uma planta alvo monocotiledônea e, opcionalmente, edição de genes de passagem. As plantas indutoras haploides monocotiledôneas são tipicamente compostas por células vegetais diploides com apenas um alelo que codifica uma proteína CENH3 funcional. As células vegetais diploides também podem incluir, por exemplo, um alelo CenH3 que codifica a proteína CENH3 não funcional. Em algumas modalidades, o alelo que codifica proteína CENH3 não funcional é uma mutação por mudança da matriz de leitura, alelo nulo de proteína, um alelo nulo de RNA ou uma combinação dos mesmos. A planta indutora haploide monocotiledônea também pode incluir um maquinário de edição de genes, tal como uma nuclease sítio-dirigida e, opcionalmente, um RNA guia expresso de maneira estável por células da planta monocotiledônea. Também são fornecidos métodos para induzir a formação de uma planta monocotiledônea haploide alvo enquanto, opcionalmente, modifica de forma simultânea o genoma da planta monocotiledônea alvo.HETEROZYGOTES MONOCOTYLEDONS FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING. These are monocot plants that are heterozygous for centromeric histone 3 (CenH3) and optionally express gene editing constructs for use in inducing haploids from a target monocot plant and optionally pass gene editing. Haploid monocot inducer plants are typically composed of diploid plant cells with only one allele encoding a functional CENH3 protein. Diploid plant cells may also include, for example, a CenH3 allele encoding the non-functional CENH3 protein. In some embodiments, the non-functional CENH3 protein-encoding allele is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The haploid monocot plant inducer may also include gene editing machinery, such as a site-directed nuclease and, optionally, a guide RNA stably expressed by cells of the monocot plant. Also provided are methods for inducing the formation of a target haploid monocot plant while optionally simultaneously modifying the genome of the target monocot plant.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202063036910P | 2020-06-09 | 2020-06-09 | |
US202063036902P | 2020-06-09 | 2020-06-09 | |
PCT/US2021/036605 WO2021252619A1 (en) | 2020-06-09 | 2021-06-09 | Heterozygous cenh3 monocots and methods of use thereof for haploid induction and simultaneous genome editing |
Publications (1)
Publication Number | Publication Date |
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BR112022025102A2 true BR112022025102A2 (en) | 2023-02-07 |
Family
ID=78846501
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022025102A BR112022025102A2 (en) | 2020-06-09 | 2021-06-09 | HETEROZYGOTES MONOCOT FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING |
Country Status (13)
Country | Link |
---|---|
US (1) | US20230270067A1 (en) |
EP (1) | EP4161253A4 (en) |
KR (1) | KR20230021743A (en) |
CN (1) | CN115811937A (en) |
AU (1) | AU2021286555A1 (en) |
BR (1) | BR112022025102A2 (en) |
CA (1) | CA3181878A1 (en) |
CL (2) | CL2022003484A1 (en) |
CO (1) | CO2022017885A2 (en) |
CR (1) | CR20220673A (en) |
IL (1) | IL298774A (en) |
MX (1) | MX2022015615A (en) |
WO (1) | WO2021252619A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20240060081A1 (en) * | 2022-08-11 | 2024-02-22 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
CN116463348B (en) * | 2023-05-26 | 2024-05-14 | 中国农业科学院作物科学研究所 | Sg RNA for editing corn ZmCENH gene by using CRISPR/Cas9 system and application thereof |
CN117305326B (en) * | 2023-11-29 | 2024-02-09 | 中国农业科学院蔬菜花卉研究所 | Broccoli BoCENH3 gene and application thereof in haploid induction |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009137135A2 (en) * | 2008-02-15 | 2009-11-12 | Zgi, Inc. | On-orbit procedures for adapting plants and animals to hostile environments |
MX2012003981A (en) * | 2009-10-06 | 2012-06-12 | Univ California | Generation of haploid plants and improved plant breeding. |
EP2697377A1 (en) * | 2011-04-15 | 2014-02-19 | Pioneer Hi-Bred International Inc. | Self-reproducing hybrid plants |
IN2015DN01829A (en) * | 2012-08-06 | 2015-05-29 | Pioneer Hi Bred Int | |
CN106998665B (en) * | 2014-08-28 | 2024-02-27 | Kws种子欧洲股份公司 | Haploid plant production |
EP3037540A1 (en) * | 2014-12-23 | 2016-06-29 | Kws Saat Se | Haploid inducer |
AU2016341041A1 (en) * | 2015-10-20 | 2018-03-15 | Pioneer Hi-Bred International, Inc. | Methods and compositions for marker-free genome modification |
NL2015753B1 (en) * | 2015-11-09 | 2017-05-26 | Rijk Zwaan Zaadteelt En Zaadhandel Bv | Non-transgenic haploid inducer lines in cucurbits. |
EP3457837A1 (en) * | 2016-05-20 | 2019-03-27 | Keygene N.V. | Method for the production of haploid and subsequent doubled haploid plants |
MX2019002907A (en) * | 2016-09-14 | 2019-09-18 | Monsanto Technology Llc | Methods and compositions for genome editing via haploid induction. |
US10519456B2 (en) * | 2016-12-02 | 2019-12-31 | Syngenta Participations Ag | Simultaneous gene editing and haploid induction |
EP3547825A4 (en) * | 2016-12-02 | 2020-07-01 | Syngenta Participations AG | Simultaneous gene editing and haploid induction |
CN106755077A (en) * | 2016-12-30 | 2017-05-31 | 华智水稻生物技术有限公司 | Using CRISPR CAS9 technologies to the method for paddy rice CENH3 site-directed point mutations |
WO2019136417A2 (en) * | 2018-01-08 | 2019-07-11 | The Regents Of The University Of California | Cenh3 deletion mutants |
CN111394386A (en) * | 2018-04-12 | 2020-07-10 | 中国水稻研究所 | Method for utilizing plant heterosis |
WO2019234129A1 (en) * | 2018-06-05 | 2019-12-12 | KWS SAAT SE & Co. KGaA | Haploid induction with modified dna-repair |
US12016286B2 (en) * | 2018-10-12 | 2024-06-25 | Syngenta Crop Protection Ag | Wheat CENH3 alleles |
-
2021
- 2021-06-09 AU AU2021286555A patent/AU2021286555A1/en active Pending
- 2021-06-09 BR BR112022025102A patent/BR112022025102A2/en unknown
- 2021-06-09 MX MX2022015615A patent/MX2022015615A/en unknown
- 2021-06-09 CA CA3181878A patent/CA3181878A1/en active Pending
- 2021-06-09 EP EP21821400.5A patent/EP4161253A4/en active Pending
- 2021-06-09 WO PCT/US2021/036605 patent/WO2021252619A1/en active Application Filing
- 2021-06-09 KR KR1020237000949A patent/KR20230021743A/en unknown
- 2021-06-09 IL IL298774A patent/IL298774A/en unknown
- 2021-06-09 CN CN202180041377.XA patent/CN115811937A/en active Pending
- 2021-06-09 CR CR20220673A patent/CR20220673A/en unknown
- 2021-06-09 US US18/001,370 patent/US20230270067A1/en active Pending
-
2022
- 2022-12-07 CL CL2022003484A patent/CL2022003484A1/en unknown
- 2022-12-09 CO CONC2022/0017885A patent/CO2022017885A2/en unknown
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2024
- 2024-01-02 CL CL2024000004A patent/CL2024000004A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
EP4161253A1 (en) | 2023-04-12 |
CA3181878A1 (en) | 2021-12-16 |
MX2022015615A (en) | 2023-03-08 |
WO2021252619A1 (en) | 2021-12-16 |
US20230270067A1 (en) | 2023-08-31 |
CO2022017885A2 (en) | 2023-02-06 |
CR20220673A (en) | 2023-05-25 |
CL2024000004A1 (en) | 2024-05-31 |
IL298774A (en) | 2023-02-01 |
CN115811937A (en) | 2023-03-17 |
AU2021286555A1 (en) | 2023-02-02 |
CL2022003484A1 (en) | 2023-07-21 |
KR20230021743A (en) | 2023-02-14 |
EP4161253A4 (en) | 2024-07-03 |
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