BR112022025102A2 - HETEROZYGOTES MONOCOT FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING - Google Patents

HETEROZYGOTES MONOCOT FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING

Info

Publication number
BR112022025102A2
BR112022025102A2 BR112022025102A BR112022025102A BR112022025102A2 BR 112022025102 A2 BR112022025102 A2 BR 112022025102A2 BR 112022025102 A BR112022025102 A BR 112022025102A BR 112022025102 A BR112022025102 A BR 112022025102A BR 112022025102 A2 BR112022025102 A2 BR 112022025102A2
Authority
BR
Brazil
Prior art keywords
cenh3
monocot
haploid
allele
methods
Prior art date
Application number
BR112022025102A
Other languages
Portuguese (pt)
Inventor
Kelly Dawe R
Jackson David
Original Assignee
Univ Georgia
Cold Spring Harbor Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Georgia, Cold Spring Harbor Laboratory filed Critical Univ Georgia
Publication of BR112022025102A2 publication Critical patent/BR112022025102A2/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MONOCOTILEDÔNEAS HETEROZIGOTAS PARA CENH3 E MÉTODOS DE USO DAS MESMAS PARA INDUÇÃO HAPLOIDE E EDIÇÃO SIMULTÂNEA DE GENOMA. Trata-se de plantas monocotiledôneas heterozigotas para histona 3 centromérica (CenH3) e, opcionalmente, que expressam construtos de edição de genes para uso na indução de haploides de uma planta alvo monocotiledônea e, opcionalmente, edição de genes de passagem. As plantas indutoras haploides monocotiledôneas são tipicamente compostas por células vegetais diploides com apenas um alelo que codifica uma proteína CENH3 funcional. As células vegetais diploides também podem incluir, por exemplo, um alelo CenH3 que codifica a proteína CENH3 não funcional. Em algumas modalidades, o alelo que codifica proteína CENH3 não funcional é uma mutação por mudança da matriz de leitura, alelo nulo de proteína, um alelo nulo de RNA ou uma combinação dos mesmos. A planta indutora haploide monocotiledônea também pode incluir um maquinário de edição de genes, tal como uma nuclease sítio-dirigida e, opcionalmente, um RNA guia expresso de maneira estável por células da planta monocotiledônea. Também são fornecidos métodos para induzir a formação de uma planta monocotiledônea haploide alvo enquanto, opcionalmente, modifica de forma simultânea o genoma da planta monocotiledônea alvo.HETEROZYGOTES MONOCOTYLEDONS FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING. These are monocot plants that are heterozygous for centromeric histone 3 (CenH3) and optionally express gene editing constructs for use in inducing haploids from a target monocot plant and optionally pass gene editing. Haploid monocot inducer plants are typically composed of diploid plant cells with only one allele encoding a functional CENH3 protein. Diploid plant cells may also include, for example, a CenH3 allele encoding the non-functional CENH3 protein. In some embodiments, the non-functional CENH3 protein-encoding allele is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The haploid monocot plant inducer may also include gene editing machinery, such as a site-directed nuclease and, optionally, a guide RNA stably expressed by cells of the monocot plant. Also provided are methods for inducing the formation of a target haploid monocot plant while optionally simultaneously modifying the genome of the target monocot plant.

BR112022025102A 2020-06-09 2021-06-09 HETEROZYGOTES MONOCOT FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING BR112022025102A2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063036910P 2020-06-09 2020-06-09
US202063036902P 2020-06-09 2020-06-09
PCT/US2021/036605 WO2021252619A1 (en) 2020-06-09 2021-06-09 Heterozygous cenh3 monocots and methods of use thereof for haploid induction and simultaneous genome editing

Publications (1)

Publication Number Publication Date
BR112022025102A2 true BR112022025102A2 (en) 2023-02-07

Family

ID=78846501

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022025102A BR112022025102A2 (en) 2020-06-09 2021-06-09 HETEROZYGOTES MONOCOT FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING

Country Status (13)

Country Link
US (1) US20230270067A1 (en)
EP (1) EP4161253A4 (en)
KR (1) KR20230021743A (en)
CN (1) CN115811937A (en)
AU (1) AU2021286555A1 (en)
BR (1) BR112022025102A2 (en)
CA (1) CA3181878A1 (en)
CL (2) CL2022003484A1 (en)
CO (1) CO2022017885A2 (en)
CR (1) CR20220673A (en)
IL (1) IL298774A (en)
MX (1) MX2022015615A (en)
WO (1) WO2021252619A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240060081A1 (en) * 2022-08-11 2024-02-22 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
CN116463348B (en) * 2023-05-26 2024-05-14 中国农业科学院作物科学研究所 Sg RNA for editing corn ZmCENH gene by using CRISPR/Cas9 system and application thereof
CN117305326B (en) * 2023-11-29 2024-02-09 中国农业科学院蔬菜花卉研究所 Broccoli BoCENH3 gene and application thereof in haploid induction

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009137135A2 (en) * 2008-02-15 2009-11-12 Zgi, Inc. On-orbit procedures for adapting plants and animals to hostile environments
MX2012003981A (en) * 2009-10-06 2012-06-12 Univ California Generation of haploid plants and improved plant breeding.
EP2697377A1 (en) * 2011-04-15 2014-02-19 Pioneer Hi-Bred International Inc. Self-reproducing hybrid plants
IN2015DN01829A (en) * 2012-08-06 2015-05-29 Pioneer Hi Bred Int
CN106998665B (en) * 2014-08-28 2024-02-27 Kws种子欧洲股份公司 Haploid plant production
EP3037540A1 (en) * 2014-12-23 2016-06-29 Kws Saat Se Haploid inducer
AU2016341041A1 (en) * 2015-10-20 2018-03-15 Pioneer Hi-Bred International, Inc. Methods and compositions for marker-free genome modification
NL2015753B1 (en) * 2015-11-09 2017-05-26 Rijk Zwaan Zaadteelt En Zaadhandel Bv Non-transgenic haploid inducer lines in cucurbits.
EP3457837A1 (en) * 2016-05-20 2019-03-27 Keygene N.V. Method for the production of haploid and subsequent doubled haploid plants
MX2019002907A (en) * 2016-09-14 2019-09-18 Monsanto Technology Llc Methods and compositions for genome editing via haploid induction.
US10519456B2 (en) * 2016-12-02 2019-12-31 Syngenta Participations Ag Simultaneous gene editing and haploid induction
EP3547825A4 (en) * 2016-12-02 2020-07-01 Syngenta Participations AG Simultaneous gene editing and haploid induction
CN106755077A (en) * 2016-12-30 2017-05-31 华智水稻生物技术有限公司 Using CRISPR CAS9 technologies to the method for paddy rice CENH3 site-directed point mutations
WO2019136417A2 (en) * 2018-01-08 2019-07-11 The Regents Of The University Of California Cenh3 deletion mutants
CN111394386A (en) * 2018-04-12 2020-07-10 中国水稻研究所 Method for utilizing plant heterosis
WO2019234129A1 (en) * 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Haploid induction with modified dna-repair
US12016286B2 (en) * 2018-10-12 2024-06-25 Syngenta Crop Protection Ag Wheat CENH3 alleles

Also Published As

Publication number Publication date
EP4161253A1 (en) 2023-04-12
CA3181878A1 (en) 2021-12-16
MX2022015615A (en) 2023-03-08
WO2021252619A1 (en) 2021-12-16
US20230270067A1 (en) 2023-08-31
CO2022017885A2 (en) 2023-02-06
CR20220673A (en) 2023-05-25
CL2024000004A1 (en) 2024-05-31
IL298774A (en) 2023-02-01
CN115811937A (en) 2023-03-17
AU2021286555A1 (en) 2023-02-02
CL2022003484A1 (en) 2023-07-21
KR20230021743A (en) 2023-02-14
EP4161253A4 (en) 2024-07-03

Similar Documents

Publication Publication Date Title
BR112022025102A2 (en) HETEROZYGOTES MONOCOT FOR CENH3 AND METHODS OF THEIR USE FOR HAPLOID INDUCTION AND SIMULTANEOUS GENOME EDITING
US11702643B2 (en) System and method for genome editing
Ye et al. Cytokinins initiate secondary growth in the Arabidopsis root through a set of LBD genes
Zhao et al. Risk associated with off-target plant genome editing and methods for its limitation
Bayne et al. RNA-directed transcriptional gene silencing in mammals
US10858662B2 (en) Genome editing with split Cas9 expressed from two vectors
Zuo et al. piRNAs and their functions in the brain
van Erp et al. The history and market impact of CRISPR RNA-guided nucleases
Fichtner et al. Precision genetic modifications: a new era in molecular biology and crop improvement
Soboleva et al. Histone variants at the transcription start-site
AU2017302551A1 (en) Variants of CRISPR from Prevotella and Francisella 1 (Cpf1)
CY1120538T1 (en) MODIFIED Ribbon Nuclear Proteins CASCADE AND THEIR USES
BR112018011242A2 (en) methods for genetic modification of plants
CO2017003428A2 (en) Nucleic acid molecules of copi delta coatomer subunits that confer resistance to coleopteran and hemiptera pests
Tsai et al. CRISPRing into the woods
BR112017017260A2 (en) dna constructs, vector, cell, plants, seed, rna expression method, and target site modification method
EA201891629A1 (en) METHODS AND COMPOSITIONS FOR IMPROVING THE EFFICIENCY OF TARGETED MODIFICATION OF GENES WITH THE APPLICATION OF MEDIATED OLIGONUCLEOTIDE REPAIR GENES
CN109689875A (en) Genome editing system and method
BR112021007403A2 (en) Compositions and methods for transgene delivery
US20240174995A1 (en) System and method for genome editing based on c2c1 nucleases
Lehmann et al. Research on genodermatoses using novel genome‐editing tools
Inacio et al. Molecular organization of the 25S–18S rDNA IGS of Fagus sylvatica and Quercus suber: a comparative analysis
JP7416745B2 (en) Modified cells, preparation methods, and constructs
Yu et al. Application of genome editing technology to microrna research in mammalians
WO2020139154A8 (en) Gene therapy dna vector and its application