CR20220673A - Monocotiledóneas heterocigotas con cenh3 y métodos de uso de estas para inducción de haploides y edición simultánea del genoma - Google Patents

Monocotiledóneas heterocigotas con cenh3 y métodos de uso de estas para inducción de haploides y edición simultánea del genoma

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Publication number
CR20220673A
CR20220673A CR20220673A CR20220673A CR20220673A CR 20220673 A CR20220673 A CR 20220673A CR 20220673 A CR20220673 A CR 20220673A CR 20220673 A CR20220673 A CR 20220673A CR 20220673 A CR20220673 A CR 20220673A
Authority
CR
Costa Rica
Prior art keywords
monocot
cenh3
plant
optionally
protein
Prior art date
Application number
CR20220673A
Other languages
English (en)
Inventor
R Kelly Dawe
David Jackson
Original Assignee
Univ Georgia
Cold Spring Harbor Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Georgia, Cold Spring Harbor Laboratory filed Critical Univ Georgia
Publication of CR20220673A publication Critical patent/CR20220673A/es

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporcionan plantas monocotiledóneas heterocigotas para la histona centromérica 3 (CenH3) y construcciones que expresan opcionalmente edición de genes, para su uso en la inducción de haploides de una planta objetivo monocotiledónea y, opcionalmente, se proporcionan ediciones de genes de transferencia. Las plantas monocotiledóneas inductoras de haploides están típicamente compuestas de células vegetales diploides que tienen solo un alelo que codifica una proteína CENH3 funcional. Las células vegetales diploides también pueden incluir, por ejemplo, un alelo CenH3 que codifica la proteína CENH3 no funcional. En algunas modalidades, el alelo que codifica la proteína CENH3 no funcional es un desplazamiento del marco de lectura, un alelo nulo de proteína, un alelo nulo de ARN o una combinación de estos. La planta monocotiledónea inductora de haploides también puede incluir maquinaria de edición de genes, tal como una nucleasa dirigida al sitio y, opcionalmente, un ARN guía expresado de manera estable por las células de la planta monocotiledónea. También se proporcionan métodos para inducir la formación de una planta monocotiledónea haploide objetivo mientras que opcionalmente se modifica simultáneamente el genoma de la planta monocotiledónea objetivo.<br /> <br />
CR20220673A 2020-06-09 2021-06-09 Monocotiledóneas heterocigotas con cenh3 y métodos de uso de estas para inducción de haploides y edición simultánea del genoma CR20220673A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063036910P 2020-06-09 2020-06-09
US202063036902P 2020-06-09 2020-06-09
PCT/US2021/036605 WO2021252619A1 (en) 2020-06-09 2021-06-09 Heterozygous cenh3 monocots and methods of use thereof for haploid induction and simultaneous genome editing

Publications (1)

Publication Number Publication Date
CR20220673A true CR20220673A (es) 2023-05-25

Family

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CR20220673A CR20220673A (es) 2020-06-09 2021-06-09 Monocotiledóneas heterocigotas con cenh3 y métodos de uso de estas para inducción de haploides y edición simultánea del genoma

Country Status (13)

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US (1) US20230270067A1 (es)
EP (1) EP4161253A1 (es)
KR (1) KR20230021743A (es)
CN (1) CN115811937A (es)
AU (1) AU2021286555A1 (es)
BR (1) BR112022025102A2 (es)
CA (1) CA3181878A1 (es)
CL (2) CL2022003484A1 (es)
CO (1) CO2022017885A2 (es)
CR (1) CR20220673A (es)
IL (1) IL298774A (es)
MX (1) MX2022015615A (es)
WO (1) WO2021252619A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024036240A1 (en) * 2022-08-11 2024-02-15 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
CN116463348B (zh) * 2023-05-26 2024-05-14 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统编辑玉米ZmCENH3基因的sg RNA及其应用
CN117305326B (zh) * 2023-11-29 2024-02-09 中国农业科学院蔬菜花卉研究所 青花菜BoCENH3基因及其在单倍体诱导中的应用

Family Cites Families (13)

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Publication number Priority date Publication date Assignee Title
WO2009137135A2 (en) * 2008-02-15 2009-11-12 Zgi, Inc. On-orbit procedures for adapting plants and animals to hostile environments
US8618354B2 (en) * 2009-10-06 2013-12-31 The Regents Of The University Of California Generation of haploid plants and improved plant breeding
US20120266324A1 (en) * 2011-04-15 2012-10-18 Pioneer Hi Bred International Inc. Self-Reproducing Hybrid Plants
US9526243B2 (en) * 2012-08-06 2016-12-27 E I Du Pont De Nemours And Company Pollination success and seed set in corn hybrid seed production
PL3186381T3 (pl) * 2014-08-28 2022-10-31 KWS SAAT SE & Co. KGaA Wytwarzanie roślin haploidalnych
CA2996329A1 (en) * 2015-10-20 2017-04-27 Pioneer Hi-Bred International, Inc. Restoring function to a non-functional gene product via guided cas systems and methods of use
NL2015753B1 (en) * 2015-11-09 2017-05-26 Rijk Zwaan Zaadteelt En Zaadhandel Bv Non-transgenic haploid inducer lines in cucurbits.
EP3457837A1 (en) * 2016-05-20 2019-03-27 Keygene N.V. Method for the production of haploid and subsequent doubled haploid plants
CN109982560A (zh) * 2016-12-02 2019-07-05 先正达参股股份有限公司 同时基因编辑和单倍体诱导
US10519456B2 (en) * 2016-12-02 2019-12-31 Syngenta Participations Ag Simultaneous gene editing and haploid induction
CN109943585B (zh) * 2018-04-12 2020-05-15 中国水稻研究所 一种利用植物杂种优势的方法
WO2019234129A1 (en) * 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Haploid induction with modified dna-repair
CA3112005A1 (en) * 2018-10-12 2020-04-16 Syngenta Crop Protection Ag Novel wheat cenh3 alleles

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CL2024000004A1 (es) 2024-05-31
EP4161253A1 (en) 2023-04-12
KR20230021743A (ko) 2023-02-14
AU2021286555A1 (en) 2023-02-02
CO2022017885A2 (es) 2023-02-06
IL298774A (en) 2023-02-01
US20230270067A1 (en) 2023-08-31
CA3181878A1 (en) 2021-12-16
CN115811937A (zh) 2023-03-17
CL2022003484A1 (es) 2023-07-21
MX2022015615A (es) 2023-03-08
WO2021252619A1 (en) 2021-12-16
BR112022025102A2 (pt) 2023-02-07

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