CO2022017885A2 - Monocotiledóneas heterocigotas con cenh3 y métodos de uso de estas para inducción de haploides y edición simultánea del genoma - Google Patents

Monocotiledóneas heterocigotas con cenh3 y métodos de uso de estas para inducción de haploides y edición simultánea del genoma

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Publication number
CO2022017885A2
CO2022017885A2 CONC2022/0017885A CO2022017885A CO2022017885A2 CO 2022017885 A2 CO2022017885 A2 CO 2022017885A2 CO 2022017885 A CO2022017885 A CO 2022017885A CO 2022017885 A2 CO2022017885 A2 CO 2022017885A2
Authority
CO
Colombia
Prior art keywords
cenh3
monocot
plant
haploid
optionally
Prior art date
Application number
CONC2022/0017885A
Other languages
English (en)
Inventor
R Kelly Dawe
David Jackson
Original Assignee
Univ Georgia
Cold Spring Harbor Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Georgia, Cold Spring Harbor Laboratory filed Critical Univ Georgia
Publication of CO2022017885A2 publication Critical patent/CO2022017885A2/es

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporcionan plantas monocotiledóneas heterocigotas para la histona centromérica 3 (CenH3) y construcciones que expresan opcionalmente edición de genes, para su uso en la inducción de haploides de una planta objetivo monocotiledónea y, opcionalmente, se proporcionan ediciones de genes de transferencia. Las plantas monocotiledóneas inductoras de haploides están típicamente compuestas de células vegetales diploides que tienen solo un alelo que codifica una proteína CENH3 funcional. Las células vegetales diploides también pueden incluir, por ejemplo, un alelo CenH3 que codifica la proteína CENH3 no funcional. En algunas modalidades, el alelo que codifica la proteína CENH3 no funcional es un desplazamiento del marco de lectura, un alelo nulo de proteína, un alelo nulo de ARN o una combinación de estos. La planta monocotiledónea inductora de haploides también puede incluir maquinaria de edición de genes, tal como una nucleasa dirigida al sitio y, opcionalmente, un ARN guía expresado de manera estable por las células de la planta monocotiledónea. También se proporcionan métodos para inducir la formación de una planta monocotiledónea haploide objetivo mientras que opcionalmente se modifica simultáneamente el genoma de la planta monocotiledónea objetivo.
CONC2022/0017885A 2020-06-09 2022-12-09 Monocotiledóneas heterocigotas con cenh3 y métodos de uso de estas para inducción de haploides y edición simultánea del genoma CO2022017885A2 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063036902P 2020-06-09 2020-06-09
US202063036910P 2020-06-09 2020-06-09
PCT/US2021/036605 WO2021252619A1 (en) 2020-06-09 2021-06-09 Heterozygous cenh3 monocots and methods of use thereof for haploid induction and simultaneous genome editing

Publications (1)

Publication Number Publication Date
CO2022017885A2 true CO2022017885A2 (es) 2023-02-06

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ID=78846501

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CONC2022/0017885A CO2022017885A2 (es) 2020-06-09 2022-12-09 Monocotiledóneas heterocigotas con cenh3 y métodos de uso de estas para inducción de haploides y edición simultánea del genoma

Country Status (13)

Country Link
US (1) US20230270067A1 (es)
EP (1) EP4161253A1 (es)
KR (1) KR20230021743A (es)
CN (1) CN115811937A (es)
AU (1) AU2021286555A1 (es)
BR (1) BR112022025102A2 (es)
CA (1) CA3181878A1 (es)
CL (1) CL2022003484A1 (es)
CO (1) CO2022017885A2 (es)
CR (1) CR20220673A (es)
IL (1) IL298774A (es)
MX (1) MX2022015615A (es)
WO (1) WO2021252619A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240060081A1 (en) * 2022-08-11 2024-02-22 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
CN116463348B (zh) * 2023-05-26 2024-05-14 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统编辑玉米ZmCENH3基因的sg RNA及其应用
CN117305326B (zh) * 2023-11-29 2024-02-09 中国农业科学院蔬菜花卉研究所 青花菜BoCENH3基因及其在单倍体诱导中的应用

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WO2009137135A2 (en) * 2008-02-15 2009-11-12 Zgi, Inc. On-orbit procedures for adapting plants and animals to hostile environments
BR112012007692A2 (pt) * 2009-10-06 2017-05-09 Univ California planta, ácido nucleico isolado, e, métodos para gerar uma planta haploide e para preparar uma planta
BR112013026600A2 (pt) * 2011-04-15 2016-12-27 Pioneer Hi Bred Int método para a produção de uma planta híbrida autorreprodutora, planta híbrida autorreprodutora, semente, cassete de supressão, planta e cassete de expressão
US9526243B2 (en) * 2012-08-06 2016-12-27 E I Du Pont De Nemours And Company Pollination success and seed set in corn hybrid seed production
CA2959566A1 (en) * 2014-08-28 2016-03-03 Kws Saat Se Plants having haploid inducing activity and their use to generate haploid plants
AU2016341041A1 (en) * 2015-10-20 2018-03-15 Pioneer Hi-Bred International, Inc. Methods and compositions for marker-free genome modification
NL2015753B1 (en) * 2015-11-09 2017-05-26 Rijk Zwaan Zaadteelt En Zaadhandel Bv Non-transgenic haploid inducer lines in cucurbits.
JP2019516373A (ja) * 2016-05-20 2019-06-20 キージーン ナムローゼ フェンノートシャップ 半数体及びそれに続く倍加半数体植物の産生方法
US10519456B2 (en) * 2016-12-02 2019-12-31 Syngenta Participations Ag Simultaneous gene editing and haploid induction
US20190376075A1 (en) * 2016-12-02 2019-12-12 Syngenta Participations Ag Simultaneous gene editing and haploid induction
CN111394386A (zh) * 2018-04-12 2020-07-10 中国水稻研究所 一种利用植物杂种优势的方法
WO2019234129A1 (en) * 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Haploid induction with modified dna-repair
CA3112005A1 (en) * 2018-10-12 2020-04-16 Syngenta Crop Protection Ag Novel wheat cenh3 alleles

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Publication number Publication date
BR112022025102A2 (pt) 2023-02-07
IL298774A (en) 2023-02-01
KR20230021743A (ko) 2023-02-14
CN115811937A (zh) 2023-03-17
AU2021286555A1 (en) 2023-02-02
US20230270067A1 (en) 2023-08-31
CA3181878A1 (en) 2021-12-16
EP4161253A1 (en) 2023-04-12
WO2021252619A1 (en) 2021-12-16
MX2022015615A (es) 2023-03-08
CL2022003484A1 (es) 2023-07-21
CR20220673A (es) 2023-05-25

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