AR083432A1 - Precursor de microarn y metodos para regular la expresion del gen objetivo - Google Patents
Precursor de microarn y metodos para regular la expresion del gen objetivoInfo
- Publication number
- AR083432A1 AR083432A1 ARP110102361A ARP110102361A AR083432A1 AR 083432 A1 AR083432 A1 AR 083432A1 AR P110102361 A ARP110102361 A AR P110102361A AR P110102361 A ARP110102361 A AR P110102361A AR 083432 A1 AR083432 A1 AR 083432A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleic acid
- acid molecule
- seq
- nematodes
- base pairs
- Prior art date
Links
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Métodos para modular la expresión del gen objetivo en plantas al expresar un precursor microARN recombinante. También el uso de dichos precursores microARN recombinantes para el control de nemátodos, en particular el control de nemátodos de quiste en soja. Además la introducción de material genético en plantas que son susceptibles a los nemátodos a fin de aumentar la resistencia a los nemátodos.Reivindicación 1: Una molécula aislada de ácido nucleico que comprende una molécula de ácido nucleico comprendido en el grupo que consiste en i) una molécula de ácido nucleico representado por SEQ ID Nº 1 y ii) una molécula de ácido nucleico con al menos 100 pares de base consecutivos de una secuencia descrita por SEQ ID Nº 1 y iii) una molécula de ácido nucleico con una identidad de al menos 70% sobre una secuencia de al menos 100 pares de bases consecutivos de ácidos nucleicos con una secuencia descrita SEQ ID Nº 1 y iv) una molécula de ácido nucleico con al menos 100 pb que hibridan en condiciones rigurosas con una molécula de ácido nucleico con al menos 100 pares de bases consecutivos de una molécula de ácido nucleico descrita por cualquiera de SEQ ID Nº 1 y v) una molécula de ácido nucleico capaz de formar una estructura secundaria homóloga a la estructura secundaria formada por SEQ ID Nº 1, vi) el complemento de cualquiera de las secuencias como se definen en i) a v).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35986410P | 2010-06-30 | 2010-06-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR083432A1 true AR083432A1 (es) | 2013-02-27 |
Family
ID=45401472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP110102361A AR083432A1 (es) | 2010-06-30 | 2011-06-30 | Precursor de microarn y metodos para regular la expresion del gen objetivo |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20130117885A1 (es) |
| EP (1) | EP2588611A4 (es) |
| CN (1) | CN102985545A (es) |
| AR (1) | AR083432A1 (es) |
| AU (1) | AU2011273004A1 (es) |
| BR (1) | BR112012033544A2 (es) |
| CA (1) | CA2801808A1 (es) |
| CL (1) | CL2012003629A1 (es) |
| WO (1) | WO2012001626A1 (es) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8097712B2 (en) | 2007-11-07 | 2012-01-17 | Beelogics Inc. | Compositions for conferring tolerance to viral disease in social insects, and the use thereof |
| US8962584B2 (en) | 2009-10-14 | 2015-02-24 | Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. | Compositions for controlling Varroa mites in bees |
| US20130047297A1 (en) | 2010-03-08 | 2013-02-21 | Robert D. Sammons | Polynucleotide molecules for gene regulation in plants |
| CN103189512A (zh) * | 2010-10-29 | 2013-07-03 | 先正达参股股份有限公司 | 用于寄生体控制的植物miRNA的过表达 |
| AU2012308686B2 (en) | 2011-09-13 | 2018-05-10 | Monsanto Technology Llc | Methods and compositions for weed control |
| US9840715B1 (en) | 2011-09-13 | 2017-12-12 | Monsanto Technology Llc | Methods and compositions for delaying senescence and improving disease tolerance and yield in plants |
| US10829828B2 (en) | 2011-09-13 | 2020-11-10 | Monsanto Technology Llc | Methods and compositions for weed control |
| MX362812B (es) | 2011-09-13 | 2019-02-13 | Monsanto Technology Llc | Metodos y composiciones para el control de malezas. |
| MX343072B (es) | 2011-09-13 | 2016-10-21 | Monsanto Technology Llc | Metodos y composiciones para controlar malezas. |
| US10806146B2 (en) | 2011-09-13 | 2020-10-20 | Monsanto Technology Llc | Methods and compositions for weed control |
| CA2848689A1 (en) | 2011-09-13 | 2013-03-21 | Monsanto Technology Llc | Methods and compositions for weed control targeting pds |
| US10760086B2 (en) | 2011-09-13 | 2020-09-01 | Monsanto Technology Llc | Methods and compositions for weed control |
| US9920326B1 (en) | 2011-09-14 | 2018-03-20 | Monsanto Technology Llc | Methods and compositions for increasing invertase activity in plants |
| IN2014MN02404A (es) | 2012-05-24 | 2015-08-21 | Seeds Ltd Ab | |
| BR112015008706A2 (pt) | 2012-10-18 | 2018-02-06 | Monsanto Technology Llc | métodos e composições para controle de praga de plantas |
| AR093909A1 (es) | 2012-12-12 | 2015-06-24 | Bayer Cropscience Ag | Uso de ingredientes activos para controlar nematodos en cultivos resistentes a nematodos |
| US10683505B2 (en) | 2013-01-01 | 2020-06-16 | Monsanto Technology Llc | Methods of introducing dsRNA to plant seeds for modulating gene expression |
| CN105358695B (zh) | 2013-01-01 | 2019-07-12 | A.B.种子有限公司 | 将dsRNA引入植物种子以调节基因表达的方法 |
| US10000767B2 (en) | 2013-01-28 | 2018-06-19 | Monsanto Technology Llc | Methods and compositions for plant pest control |
| EP2967082A4 (en) | 2013-03-13 | 2016-11-02 | Monsanto Technology Llc | METHOD AND COMPOSITIONS FOR WEED CONTROL |
| CA2905027A1 (en) | 2013-03-13 | 2014-10-09 | Monsanto Technology Llc | Methods and compositions for weed control |
| US20140283211A1 (en) | 2013-03-14 | 2014-09-18 | Monsanto Technology Llc | Methods and Compositions for Plant Pest Control |
| US10568328B2 (en) | 2013-03-15 | 2020-02-25 | Monsanto Technology Llc | Methods and compositions for weed control |
| MX359191B (es) | 2013-07-19 | 2018-09-18 | Monsanto Technology Llc | Composiciones y métodos para controlar leptinotarsa. |
| US9850496B2 (en) | 2013-07-19 | 2017-12-26 | Monsanto Technology Llc | Compositions and methods for controlling Leptinotarsa |
| CN104450770B (zh) * | 2013-09-25 | 2017-08-15 | 中国林业科学研究院林业研究所 | 落叶松miR166a在调控植物发育中的应用 |
| MX2016005778A (es) | 2013-11-04 | 2016-12-20 | Monsanto Technology Llc | Composiciones y metodos para controlar infestaciones de plagas y parasitos de los artropodos. |
| UA119253C2 (uk) | 2013-12-10 | 2019-05-27 | Біолоджикс, Інк. | Спосіб боротьби із вірусом у кліща varroa та у бджіл |
| AU2015206585A1 (en) | 2014-01-15 | 2016-07-21 | Monsanto Technology Llc | Methods and compositions for weed control using EPSPS polynucleotides |
| EP3125676A4 (en) | 2014-04-01 | 2018-02-14 | Monsanto Technology LLC | Compositions and methods for controlling insect pests |
| CA2953347A1 (en) | 2014-06-23 | 2015-12-30 | Monsanto Technology Llc | Compositions and methods for regulating gene expression via rna interference |
| WO2015200539A1 (en) | 2014-06-25 | 2015-12-30 | Monsanto Technology Llc | Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression |
| EP3174982A4 (en) | 2014-07-29 | 2018-06-20 | Monsanto Technology LLC | Compositions and methods for controlling insect pests |
| RU2723049C2 (ru) | 2015-01-22 | 2020-06-08 | Монсанто Текнолоджи Ллс | Композиции и способы борьбы с leptinotarsa |
| UY36703A (es) | 2015-06-02 | 2016-12-30 | Monsanto Technology Llc | Composiciones y métodos para la administración de un polinucleótido en una planta |
| CN108024517A (zh) | 2015-06-03 | 2018-05-11 | 孟山都技术公司 | 用于将核酸引入到植物中的方法和组合物 |
| WO2017019935A1 (en) * | 2015-07-30 | 2017-02-02 | Modernatx, Inc. | Multimeric mrna |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2583690C (en) * | 2004-10-12 | 2016-04-05 | The Rockefeller University | Microrna constructs for the suppression of the expression of targeted genes or the down regulation of targeted genes and methods therefore |
| EP2163638A1 (en) * | 2004-12-20 | 2010-03-17 | BASF Plant Science GmbH | Nucleic acid molecules encoding fatty acid desaturase genes from plants and methods of use |
| CN101225394A (zh) * | 2007-01-18 | 2008-07-23 | 中国科学院上海生命科学研究院 | 植物miRNA及其用途 |
| AU2008218813B2 (en) * | 2007-02-20 | 2014-04-17 | Monsanto Technology, Llc | Invertebrate microRNAs |
| EP2171065A2 (en) * | 2007-06-15 | 2010-04-07 | BASF Plant Science GmbH | Compositions and methods of using rna interference for control of nematodes |
-
2011
- 2011-06-28 EP EP11800280.7A patent/EP2588611A4/en not_active Withdrawn
- 2011-06-28 AU AU2011273004A patent/AU2011273004A1/en not_active Abandoned
- 2011-06-28 CN CN2011800322989A patent/CN102985545A/zh active Pending
- 2011-06-28 BR BR112012033544A patent/BR112012033544A2/pt not_active IP Right Cessation
- 2011-06-28 CA CA2801808A patent/CA2801808A1/en not_active Abandoned
- 2011-06-28 US US13/807,412 patent/US20130117885A1/en not_active Abandoned
- 2011-06-28 WO PCT/IB2011/052837 patent/WO2012001626A1/en active Application Filing
- 2011-06-30 AR ARP110102361A patent/AR083432A1/es unknown
-
2012
- 2012-12-20 CL CL2012003629A patent/CL2012003629A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN102985545A (zh) | 2013-03-20 |
| CA2801808A1 (en) | 2012-01-05 |
| EP2588611A1 (en) | 2013-05-08 |
| BR112012033544A2 (pt) | 2015-09-08 |
| WO2012001626A1 (en) | 2012-01-05 |
| CL2012003629A1 (es) | 2013-05-17 |
| US20130117885A1 (en) | 2013-05-09 |
| AU2011273004A1 (en) | 2015-11-19 |
| EP2588611A4 (en) | 2014-01-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |