RU2014127702A - Модифицированные Cascade-рибонуклеопротеины и их применения - Google Patents
Модифицированные Cascade-рибонуклеопротеины и их применения Download PDFInfo
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- 102000004389 Ribonucleoproteins Human genes 0.000 title claims abstract 3
- 108010081734 Ribonucleoproteins Proteins 0.000 title claims abstract 3
- 238000000034 method Methods 0.000 claims abstract 41
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 27
- 108020004707 nucleic acids Proteins 0.000 claims abstract 26
- 102000039446 nucleic acids Human genes 0.000 claims abstract 26
- 210000004027 cell Anatomy 0.000 claims abstract 15
- 108020004414 DNA Proteins 0.000 claims abstract 10
- 108091079001 CRISPR RNA Proteins 0.000 claims abstract 8
- 102000053602 DNA Human genes 0.000 claims abstract 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims abstract 6
- 210000003527 eukaryotic cell Anatomy 0.000 claims abstract 5
- 108020004682 Single-Stranded DNA Proteins 0.000 claims abstract 2
- 238000011065 in-situ storage Methods 0.000 claims abstract 2
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 210000000130 stem cell Anatomy 0.000 claims 4
- 238000012986 modification Methods 0.000 claims 3
- 230000004048 modification Effects 0.000 claims 3
- 239000013598 vector Substances 0.000 claims 3
- 210000001161 mammalian embryo Anatomy 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 125000006850 spacer group Chemical group 0.000 claims 2
- 241000238631 Hexapoda Species 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 230000006801 homologous recombination Effects 0.000 claims 1
- 238000002744 homologous recombination Methods 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
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Abstract
1. Способ модификации целевой нуклеиновой кислоты in situ в эукариотической клетке, включающий:(а) приведение указанной целевой нуклеиновой кислоты в контакт с рибонуклеопротеином, содержащим CRISPR РНК (производное РНК и кластерных коротких палиндромных повторов, разделенных регулярными промежутками); и(б) модификацию указанной целевой нуклеиновой кислоты в указанной эукариотической клетке.2. Способ по п. 1, где указанная целевая нуклеиновая кислота содержит мотив, примыкающий к протоспейсеру, расположенный рядом с последовательностью, с которой гибридизуется CRISP РНК.3. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, маркирует точку в указанной целевой нуклеиновой кислоте, в которой происходит спаривание оснований с указанной CRISPR РНК.4. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, содержит 5′-ССТ-3′.5. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, содержит 5′-СТТ-3′.6. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, содержит 5′-САТ-3′.7. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, содержит 5′-СТС-3′.8. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, представляет собой 5′-ССТ-3′.9. Способ по п. 1, где указанную CRISPR РНК получают путем экспрессии в генетически модифицированных клетках.10. Способ по п. 1, где указанная целевая нуклеиновая кислота представляет собой двухцепочечную нуклеиновую кислоту.11. Способ по п. 1, где указанная целевая нуклеиновая кислота представляет собой одноцепочечную ДНК.12. Способ по п. 1, где указанная целевая нуклеиновая кислота представляет собой двухцепочечную ДНК.13. Способ по п. 1, где указанная целевая нуклеиновая кислота представляет собой геномную ДНК.14. Способ по п. 1, г
Claims (27)
1. Способ модификации целевой нуклеиновой кислоты in situ в эукариотической клетке, включающий:
(а) приведение указанной целевой нуклеиновой кислоты в контакт с рибонуклеопротеином, содержащим CRISPR РНК (производное РНК и кластерных коротких палиндромных повторов, разделенных регулярными промежутками); и
(б) модификацию указанной целевой нуклеиновой кислоты в указанной эукариотической клетке.
2. Способ по п. 1, где указанная целевая нуклеиновая кислота содержит мотив, примыкающий к протоспейсеру, расположенный рядом с последовательностью, с которой гибридизуется CRISP РНК.
3. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, маркирует точку в указанной целевой нуклеиновой кислоте, в которой происходит спаривание оснований с указанной CRISPR РНК.
4. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, содержит 5′-ССТ-3′.
5. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, содержит 5′-СТТ-3′.
6. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, содержит 5′-САТ-3′.
7. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, содержит 5′-СТС-3′.
8. Способ по п. 2, где указанный мотив, примыкающий к протоспейсеру, представляет собой 5′-ССТ-3′.
9. Способ по п. 1, где указанную CRISPR РНК получают путем экспрессии в генетически модифицированных клетках.
10. Способ по п. 1, где указанная целевая нуклеиновая кислота представляет собой двухцепочечную нуклеиновую кислоту.
11. Способ по п. 1, где указанная целевая нуклеиновая кислота представляет собой одноцепочечную ДНК.
12. Способ по п. 1, где указанная целевая нуклеиновая кислота представляет собой двухцепочечную ДНК.
13. Способ по п. 1, где указанная целевая нуклеиновая кислота представляет собой геномную ДНК.
14. Способ по п. 1, где спейсерный участок CRISPR РНК имеет по меньшей мере 50%-ную идентичность с целевой нуклеиновокислотной последовательностью.
15. Способ по п. 14, где спейсерный участок CRISPR РНК имеет 32 нуклеотида.
16. Способ по п. 1, где указанная модификация включает расщепление указанной целевой нуклеиновой кислоты.
17. Способ по п. 1, где указанная модификация включает негомологичное соединения концов молекулы дцДНК в клетке в нужном локусе для удаления по меньшей мере части нуклеотидной последовательности из этой молекулы ДЦДНК.
18. Способ по п. 1, где указанная модификация включает гомологичную рекомбинацию нуклеиновой кислоты в молекулу дцДНК в клетке в нужном локусе.
19. Способ по п. 1, где указанная клетка представляет собой клетку насекомого.
20. Способ по п. 1, где указанная клетка представляет собой клетку млекопитающего.
21. Способ по п. 20, где указанная клетка представляет собой стволовую клетку млекопитающего, не являющуюся стволовой клеткой человеческого эмбриона.
22. Способ по п. 20, где указанная клетка представляет собой клетку человека.
23. Способ по п. 22, где указанная клетка представляет собой стволовую клетку человека, не являющуюся стволовой клеткой человеческого эмбриона.
24. Способ по п. 1, где указанная клетка представляет собой клетку растения.
25. Способ по п. 1, где указанная клетка представляет собой дрожжевую клетку.
26. Способ по п. 1, где указанная клетка представляет собой клетку гриба.
27. Способ изменения экспрессии целевой нуклеиновой кислоты, включающий введение в эукариотическую клетку, содержащую молекулу ДНК, кодирующую указанную целевую нуклеиновую кислоту, сконструированной системы кластерных коротких палиндромных повторов, разделенных регулярными промежутками, содержащей один или более векторов, содержащих: (а) первую нуклеотидную последовательность, кодирующую РНК со сконструированными кластерными короткими палиндромными повторами, разделенными регулярными промежутками, которая гибридизуется с целевой нуклеиновой кислотой, и б) вторую нуклеотидную последовательность, кодирующую пептид, ассоциированный с кластерными короткими палиндромными повторами, разделенными регулярными промежутками, где компоненты (а) и (б) находятся в одном векторе или в разных векторах этой системы, посредством чего указанная РНК нацеливается на указанную нуклеиновую кислоту, и указанный пептид, ассоциированный с кластерными короткими палиндромными повторами, разделенными регулярными промежутками, расщепляет указанную молекулу ДНК.
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