AR098299A1 - Locus óptimos del maíz - Google Patents

Locus óptimos del maíz

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Publication number
AR098299A1
AR098299A1 ARP140104138A ARP140104138A AR098299A1 AR 098299 A1 AR098299 A1 AR 098299A1 AR P140104138 A ARP140104138 A AR P140104138A AR P140104138 A ARP140104138 A AR P140104138A AR 098299 A1 AR098299 A1 AR 098299A1
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AR
Argentina
Prior art keywords
seq
loci
sequence
dna
interest
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ARP140104138A
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English (en)
Inventor
Sriram Shreedharan
L Camper Debra
Sastry-Dent Lakshmi
R Webb Steven
Cao Zehui
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Dow Agrosciences Llc
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Publication of AR098299A1 publication Critical patent/AR098299A1/es

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
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    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

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  • Pest Control & Pesticides (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

Reivindicación 1: Una secuencia recombinante, donde dicha secuencia recombinante comprende: una secuencia de ácido nucleico de al menos 1 Kb y que tiene una identidad de secuencia de al menos 90% con una secuencia no génica que se selecciona del grupo que consiste en loci_137693_G1 (SEQ ID Nº 387), loci_265551_G1 (SEQ ID Nº 463), loci_128078_G1 (SEQ ID Nº 560), loci_168286_G1 (SEQ ID Nº 573), loci_3733_G1 (SEQ ID Nº 1268), loci_203075_G1 (SEQ ID Nº 2030), loci_232484_G1 (SEQ ID Nº 2053), loci_136086_G1 (SEQ ID Nº 4425), loci_203704_G1 (SEQ ID Nº 2033), loci_127268_G1 (SEQ ID Nº 2709), loci_204637_G1 (SEQ ID Nº 2731), loci_291068_G1 (SEQ ID Nº 3230), loci_232222_G1 (SEQ ID Nº 3357), loci_43577_G1 (SEQ ID Nº 3428), loci_204726_G1 (SEQ ID Nº 424), loci_232228_G1 (SEQ ID Nº 4529); y, un ADN de interés, donde el ADN de interés se inserta en dicha secuencia no génica para producir dicha secuencia recombinante. Reivindicación 15: Una célula de planta de maíz, parte de una planta de maíz o planta de maíz que comprende una secuencia recombinante de cualquiera de las reivindicaciones 1 - 10. Reivindicación 16: Un método para elaborar una célula vegetal transgénica que comprende un ADN de interés donde el método comprende: a) seleccionar un locus genómico optimo no génico de maíz objetivo que tiene una identidad de secuencia del al menos 90% del grupo que consiste en loci_137693_G1 (SEQ ID Nº 387), loci_265551_G1 (SEQ ID Nº 463), loci_128078_G1 (SEQ ID Nº 560) loci_168286_G1 (SEQ ID Nº 573), loci_3733_G1 (SEQ ID Nº 1268), loci_203075_G1 (SEQ ID Nº 2030), loci_232484_G1 (SEQ ID Nº 2053), loci_136086_G1 (SEQ ID Nº 4425), loci_203704_G1 (SEQ ID Nº 2033), loci_127268_G1 (SEQ ID Nº 2709), loci_204637_G1 (SEQ ID Nº 2731), loci_291068_G1 (SEQ ID Nº 3230), loci_232222_G1 (SEQ ID Nº 3357), loci_43577_G1 (SEQ ID Nº 3428), loci_204726_G1 (SEQ ID Nº 424) y loci_232228_G1 (SEQ ID Nº 4529); b) seleccionar una nucleasa específica del sitio que se una específicamente y escinda dicho locus genómico no génico de maíz objetivo; c) introducir dicha nucleasa específica del sitio en una célula vegetal de maíz; d) introducir el ADN de interés en la célula vegetal; e) insertar el ADN de interés en dicho locus genómico no génico de maíz objetivo; y f) seleccionar células vegetales transgénicas que comprenden el ADN de interés direccionado a dichos locus no génicos. Reivindicación 28: Un locus genómico no génico de maíz purificado de al menos 1 Kb y que tiene una identidad de secuencia de al menos 90 % con una secuencia no génica que se selecciona del grupo que consiste en loci_137693_G1 (SEQ ID Nº 387), loci_265551_G1 (SEQ ID Nº 463), loci_128078_G1 (SEQ ID Nº 560), loci_168286_G1 (SEQ ID Nº 573), loci_3733_G1 (SEQ ID Nº 1268), loci_203075_G1 (SEQ ID Nº 2030), loci_232484_G1 (SEQ ID Nº 2053), loci_136086_G1 (SEQ ID Nº 4425), loci_203704_G1 (SEQ ID Nº 2033), loci_127268_G1 (SEQ ID Nº 2709), loci_204637_G1 (SEQ ID Nº 2731), loci_291068_G1 (SEQ ID Nº 3230), loci_232222_G1 (SEQ ID Nº 3357), loci_43577_G1 (SEQ ID Nº 3428), loci_204726_G1 (SEQ ID Nº 424), y loci_232228_G1 (SEQ ID Nº 4529). Reivindicación 43: Una planta que comprende una secuencia recombinante, donde dicha secuencia recombinante comprende: una secuencia de ácido nucleico que tiene una identidad de secuencia de al menos 90% con una secuencia no génica, y un ADN de interés, donde el ADN de interés se inserta en dicha secuencia no génica.
ARP140104138A 2013-11-04 2014-11-04 Locus óptimos del maíz AR098299A1 (es)

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US (3) US10093940B2 (es)
EP (1) EP3066110B1 (es)
JP (2) JP6649261B2 (es)
KR (1) KR102269371B1 (es)
CN (1) CN106164085A (es)
AP (1) AP2016009227A0 (es)
AR (1) AR098299A1 (es)
AU (3) AU2014341927B2 (es)
BR (1) BR102014027442B1 (es)
CA (1) CA2928855A1 (es)
CL (1) CL2016001065A1 (es)
IL (1) IL245351B (es)
MX (1) MX360318B (es)
NZ (1) NZ719494A (es)
RU (2) RU2019128647A (es)
TW (2) TWI721478B (es)
UA (1) UA120503C2 (es)
UY (1) UY35815A (es)
WO (1) WO2015066636A2 (es)
ZA (1) ZA201602979B (es)

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