RU2016104056A - Мультиплексная геномная инженерия, направляемая рнк - Google Patents
Мультиплексная геномная инженерия, направляемая рнк Download PDFInfo
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- RU2016104056A RU2016104056A RU2016104056A RU2016104056A RU2016104056A RU 2016104056 A RU2016104056 A RU 2016104056A RU 2016104056 A RU2016104056 A RU 2016104056A RU 2016104056 A RU2016104056 A RU 2016104056A RU 2016104056 A RU2016104056 A RU 2016104056A
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- dna
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- 108020004414 DNA Proteins 0.000 claims 13
- 238000000034 method Methods 0.000 claims 12
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 11
- 210000004027 cell Anatomy 0.000 claims 8
- 150000007523 nucleic acids Chemical class 0.000 claims 8
- 108090000790 Enzymes Proteins 0.000 claims 7
- 102000004190 Enzymes Human genes 0.000 claims 7
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims 3
- 230000008045 co-localization Effects 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 108091033409 CRISPR Proteins 0.000 claims 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 1
- 101710096438 DNA-binding protein Proteins 0.000 claims 1
- 108091029865 Exogenous DNA Proteins 0.000 claims 1
- 108020005196 Mitochondrial DNA Proteins 0.000 claims 1
- 108020005202 Viral DNA Proteins 0.000 claims 1
- 210000004102 animal cell Anatomy 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 230000004927 fusion Effects 0.000 claims 1
- 230000006801 homologous recombination Effects 0.000 claims 1
- 238000002744 homologous recombination Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 230000006798 recombination Effects 0.000 claims 1
- 238000005215 recombination Methods 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
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- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Crystallography & Structural Chemistry (AREA)
Claims (18)
1. Способ внесения множественных изменений в ДНК-мишень в клетке, экспрессирующей фермент, который образует колокализационный комплекс с РНК, комплементарной ДНК-мишени, и который расщепляет ДНК-мишень сайт-специфическим образом, включающий
(а) введение в клетку первой чужеродной нуклеиновой кислоты, кодирующей одну или несколько РНК, комплементарных ДНК-мишени и направляющих фермент к ДНК-мишени, в котором одна или несколько РНК и фермент являются членами колокализационного комплекса для ДНК-мишени,
введение в клетку второй чужеродной нуклеиновой кислоты, кодирующей одну или несколько последовательностей донорной нуклеиновой кислоты,
где одна или несколько РНК и одна или несколько последовательностей донорной нуклеиновой кислоты экспрессируются,
где одна или несколько РНК и фермент колокализуются на ДНК-мишени, фермент расщепляет ДНК-мишень, и донорная нуклеиновая кислота вставляется в ДНК-мишень для получения измененной ДНК в клетке, и
повторение стадии (а) множество раз для внесения множественных изменений в ДНК в клетке.
2. Способ по п. 1, в котором фермент представляет собой РНК-направляемый ДНК-связывающий белок.
3. Способ по п. 1, в котором ферментом является Cas9.
4. Способ по п. 1, в котором клетка является эукариотической клеткой.
5. Способ по п. 1, в котором клетка является клеткой дрожжей, клеткой растений или клеткой животных.
6. Способ по п. 1, в котором РНК содержит примерно от 10 до 500 нуклеотидов.
7. Способ по п. 1, в котором РНК содержит примерно от 20 до 100 нуклеотидов.
8. Способ по п. 1, в котором одна или несколько РНК являются направляющими РНК.
9. Способ по п. 1, в котором одна или несколько РНК являются слиянием tracrRNA-crRNA.
10. Способ по п. 1, в котором ДНК является геномной ДНК, митохондриальной ДНК, вирусной ДНК или экзогенной ДНК.
11. Способ по п. 1, в котором вставка одной или нескольких последовательностей донорной нуклеиновой кислоты осуществляется рекомбинацией.
12. Способ по п. 1, в котором вставка одной или нескольких последовательностей донорной нуклеиновой кислоты осуществляется гомологичной рекомбинацией.
13. Способ по п. 1, в котором одна или несколько РНК и одна или несколько последовательностей донорной нуклеиновой кислоты присутствуют на одной или нескольких плазмидах.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361844168P | 2013-07-09 | 2013-07-09 | |
US61/844,168 | 2013-07-09 | ||
PCT/US2014/045691 WO2015006290A1 (en) | 2013-07-09 | 2014-07-08 | Multiplex rna-guided genome engineering |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
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RU2019135885A Division RU2764637C2 (ru) | 2013-07-09 | 2014-07-08 | Мультиплексная геномная инженерия, направляемая рнк |
Publications (3)
Publication Number | Publication Date |
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RU2016104056A true RU2016104056A (ru) | 2017-08-14 |
RU2016104056A3 RU2016104056A3 (ru) | 2018-03-21 |
RU2706562C2 RU2706562C2 (ru) | 2019-11-19 |
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RU2016104056A RU2706562C2 (ru) | 2013-07-09 | 2014-07-08 | Мультиплексная геномная инженерия, направляемая рнк |
RU2019135885A RU2764637C2 (ru) | 2013-07-09 | 2014-07-08 | Мультиплексная геномная инженерия, направляемая рнк |
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RU2019135885A RU2764637C2 (ru) | 2013-07-09 | 2014-07-08 | Мультиплексная геномная инженерия, направляемая рнк |
Country Status (13)
Country | Link |
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US (2) | US11459585B2 (ru) |
EP (2) | EP4166669A1 (ru) |
JP (3) | JP7120717B2 (ru) |
KR (3) | KR20230122182A (ru) |
CN (2) | CN105518144A (ru) |
AU (3) | AU2014287393B2 (ru) |
CA (2) | CA2917638A1 (ru) |
ES (1) | ES2929143T3 (ru) |
HK (1) | HK1217967A1 (ru) |
IL (2) | IL243475B2 (ru) |
RU (2) | RU2706562C2 (ru) |
SG (3) | SG10201800111SA (ru) |
WO (1) | WO2015006290A1 (ru) |
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