MX364830B - Ribonucleoproteinas de cascada modificadas y usos de las mismas. - Google Patents

Ribonucleoproteinas de cascada modificadas y usos de las mismas.

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Publication number
MX364830B
MX364830B MX2014007910A MX2014007910A MX364830B MX 364830 B MX364830 B MX 364830B MX 2014007910 A MX2014007910 A MX 2014007910A MX 2014007910 A MX2014007910 A MX 2014007910A MX 364830 B MX364830 B MX 364830B
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Mexico
Prior art keywords
complex
cascade
crispr
rna molecule
sequence
Prior art date
Application number
MX2014007910A
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English (en)
Other versions
MX2014007910A (es
Inventor
Stan Johan Jozef Brouns
Der Oost John Van
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Caribou Biosciences Inc
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Publication date
Application filed by Caribou Biosciences Inc filed Critical Caribou Biosciences Inc
Publication of MX2014007910A publication Critical patent/MX2014007910A/es
Publication of MX364830B publication Critical patent/MX364830B/es

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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21004—Type II site-specific deoxyribonuclease (3.1.21.4)

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  • Biotechnology (AREA)
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  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La presente invención se refiere a un complejo asociado de repetición palindrómica corta espaciado regularmente agrupado (CRISPR = clustered regularly interspaced short palindromic repeat) para la defensa antiviral adaptativa (Cascada); el complejo de proteína cascada que comprende al menos subunidades de proteínas asociadas-CRISPR Cas7, Cas5 y Cas6 que incluye al menos una subunidad con una secuencia de aminoácidos adicional que posee actividad represora de transcripción o de activación de transcripción, visualización, modificación de cromatina o ácido nucleico. El complejo Cascada con actividad adicional se combina con una molécula de ARN para producir un complejo de ribonucleoproteína. La molécula de ARN se selecciona para que tenga complementariedad sustancial con una secuencia diana. Ribonucleoproteínas dirigidas pueden ser utilizadas como herramientas de ingeniería genética para el corte preciso de los ácidos nucleicos en la recombinación homóloga, unión de extremos no homólogos, modificación de genes, integración de genes, reparación de mutación o para su visualización, la activación o represión transcripcional. Un par de ribonucleótidos fusionados a dímeros FokI puede ser utilizado para generar rupturas de doble hebra en el ADN para facilitar estas aplicaciones de una manera específica de secuencia.
MX2014007910A 2011-12-30 2012-12-21 Ribonucleoproteinas de cascada modificadas y usos de las mismas. MX364830B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201122458A GB201122458D0 (en) 2011-12-30 2011-12-30 Modified cascade ribonucleoproteins and uses thereof
PCT/EP2012/076674 WO2013098244A1 (en) 2011-12-30 2012-12-21 Modified cascade ribonucleoproteins and uses thereof

Publications (2)

Publication Number Publication Date
MX2014007910A MX2014007910A (es) 2015-02-12
MX364830B true MX364830B (es) 2019-05-08

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MX2014007910A MX364830B (es) 2011-12-30 2012-12-21 Ribonucleoproteinas de cascada modificadas y usos de las mismas.

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US (11) US20140294773A1 (es)
EP (2) EP2798060A1 (es)
JP (1) JP6408914B2 (es)
KR (1) KR101889589B1 (es)
CN (2) CN104321429A (es)
AU (1) AU2012360975B2 (es)
BR (1) BR112014016228B1 (es)
CA (1) CA2862018C (es)
CY (1) CY1120538T1 (es)
DE (1) DE212012000234U1 (es)
DK (1) DK3091072T3 (es)
ES (1) ES2689256T3 (es)
GB (3) GB201122458D0 (es)
HR (1) HRP20181150T1 (es)
HU (1) HUE039617T2 (es)
IL (1) IL233399A0 (es)
IN (1) IN2014DN05937A (es)
LT (1) LT3091072T (es)
MX (1) MX364830B (es)
PL (1) PL3091072T3 (es)
PT (1) PT3091072T (es)
RS (1) RS57604B1 (es)
RU (1) RU2014127702A (es)
SG (1) SG11201403713QA (es)
SI (1) SI3091072T1 (es)
SM (1) SMT201800498T1 (es)
WO (1) WO2013098244A1 (es)

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