MX363842B - Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas. - Google Patents
Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas.Info
- Publication number
- MX363842B MX363842B MX2016002119A MX2016002119A MX363842B MX 363842 B MX363842 B MX 363842B MX 2016002119 A MX2016002119 A MX 2016002119A MX 2016002119 A MX2016002119 A MX 2016002119A MX 363842 B MX363842 B MX 363842B
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
Se proporcionan composiciones y métodos para modificar el genoma de una secuencia objetivo en el genoma de una planta o célula vegetal, sin incorporar un marcador transgénico seleccionable. Los métodos y composiciones usan un sistema polinucleótido guía/endonucleasa Cas para proporcionar un sistema eficaz para modificar o alterar sitios objetivo dentro del genoma de una planta, célula vegetal o semilla sin incorporar un marcador transgénico seleccionable. Una vez que se identifica un sitio objetivo genómico, puede emplearse una variedad de métodos para modificar aún más los sitios objetivo de manera que contengan una variedad de polinucleótidos de interés. Se describen, además, métodos de reproducción y métodos para seleccionar plantas con el uso de un sistema polinucleótido guía/endonucleasa Cas. Además, se proporcionan composiciones y métodos para editar una secuencia de nucleótidos en el genoma de una célula, sin incorporar un marcador transgénico seleccionable.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361868706P | 2013-08-22 | 2013-08-22 | |
| US201361882532P | 2013-09-25 | 2013-09-25 | |
| US201461937045P | 2014-02-07 | 2014-02-07 | |
| US201461953090P | 2014-03-14 | 2014-03-14 | |
| US201462023239P | 2014-07-11 | 2014-07-11 | |
| PCT/US2014/051781 WO2015026886A1 (en) | 2013-08-22 | 2014-08-20 | Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker, and compositions thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016002119A MX2016002119A (es) | 2016-06-23 |
| MX363842B true MX363842B (es) | 2019-04-05 |
Family
ID=51493053
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016002119A MX363842B (es) | 2013-08-22 | 2014-08-20 | Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas. |
| MX2016002306A MX2016002306A (es) | 2013-08-22 | 2014-08-20 | Promotor u6 de polimerasa iii de soja y metodos de uso. |
| MX2016002266A MX361154B (es) | 2013-08-22 | 2014-08-20 | Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso. |
| MX2016002118A MX2016002118A (es) | 2013-08-22 | 2014-08-20 | Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso. |
| MX2018014501A MX2018014501A (es) | 2013-08-22 | 2016-02-19 | Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso. |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016002306A MX2016002306A (es) | 2013-08-22 | 2014-08-20 | Promotor u6 de polimerasa iii de soja y metodos de uso. |
| MX2016002266A MX361154B (es) | 2013-08-22 | 2014-08-20 | Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso. |
| MX2016002118A MX2016002118A (es) | 2013-08-22 | 2014-08-20 | Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso. |
| MX2018014501A MX2018014501A (es) | 2013-08-22 | 2016-02-19 | Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso. |
Country Status (12)
| Country | Link |
|---|---|
| US (17) | US20160201072A1 (es) |
| EP (6) | EP3611268A1 (es) |
| CN (5) | CN106133138A (es) |
| AU (6) | AU2014308899B2 (es) |
| BR (3) | BR112016003561B8 (es) |
| CA (7) | CA3157565A1 (es) |
| DK (1) | DK3036327T3 (es) |
| ES (1) | ES2729635T3 (es) |
| HU (1) | HUE044916T2 (es) |
| LT (1) | LT3036327T (es) |
| MX (5) | MX363842B (es) |
| WO (4) | WO2015026886A1 (es) |
Families Citing this family (347)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112012032907A2 (pt) | 2010-06-25 | 2017-06-13 | Du Pont | métodos para selecionar e identificar uma planta de mais e planta de mais |
| BR112013024337A2 (pt) | 2011-03-23 | 2017-09-26 | Du Pont | locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão |
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