BR112014018560A8 - Planta ou semente, método para produzir uma segunda planta, método de produção de um locus, método de alteração de um locus, método de gerar uma biblioteca de plantas, método de identificar plantas, biblioteca de plantas, plantas de progênie - Google Patents

Planta ou semente, método para produzir uma segunda planta, método de produção de um locus, método de alteração de um locus, método de gerar uma biblioteca de plantas, método de identificar plantas, biblioteca de plantas, plantas de progênie

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Publication number
BR112014018560A8
BR112014018560A8 BR112014018560A BR112014018560A BR112014018560A8 BR 112014018560 A8 BR112014018560 A8 BR 112014018560A8 BR 112014018560 A BR112014018560 A BR 112014018560A BR 112014018560 A BR112014018560 A BR 112014018560A BR 112014018560 A8 BR112014018560 A8 BR 112014018560A8
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BR
Brazil
Prior art keywords
plant
producing
genomic
locu
interest
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BR112014018560A
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English (en)
Other versions
BR112014018560A2 (pt
Inventor
Carl Falco Saverio
Lassner Michael
Li Zhongsen
J Scelonge Christopher
Original Assignee
Pioneer Hi Bred Int
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Pioneer Hi Bred Int, Du Pont filed Critical Pioneer Hi Bred Int
Publication of BR112014018560A2 publication Critical patent/BR112014018560A2/pt
Publication of BR112014018560A8 publication Critical patent/BR112014018560A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)

Abstract

MÉTODO PARA PRODUZIR UMA SEGUNDA PLANTA, MÉTODO DE PRODUÇÃO DE UM LÓCUS, MÉTODO DE ALTERAÇÃO DE UM LÓCUS, MÉTODO DE GERAR UMA BIBLIOTECA DE PLANTAS, MÉTODO DE IDENTIFICAR PLANTAS, MÉTODO DE OBTENÇÃO DE UMA PLANTA TRANSGÊNICA Trata-se de composições e métodos para empilhar múltiplos loci transgênicos independentes no genoma de uma planta. As composições incluem plantas, sementes ou células de planta que compreendem pelo menos um sítio-alvo transgênico e pelo menos um locus genômico de interesse integrado a diferentes sítios genômicos em uma janela genômica. As técnicas de reprodução através de melhoramento de planta podem ser empregadas de modo que o sítio-alvo transgênico e o locus genômico de interesse possam ser juntamente reproduzidos através de melhoramento. Dessa forma, podem ser geradas múltiplas integrações de transgene independentes em uma janela genômica a fim de criar um locus de traço complexo. O locus de traço complexo é projetado de modo que os sítios alvos transgênicos e/ou loci genômicos de interesse possam se segregar independentemente um do outro, fornecendo assim o benefício de alteração de um locus de traço complexo por meio da inserção através de melhoramento e da retirada através de melhoramento de elementos específicos. Vários métodos também podem ser empregados para modificar os sítios alvos de modo que os mesmos contenham uma variedade de polinucleotídeos de interesse.
BR112014018560A 2012-01-27 2013-01-24 Planta ou semente, método para produzir uma segunda planta, método de produção de um locus, método de alteração de um locus, método de gerar uma biblioteca de plantas, método de identificar plantas, biblioteca de plantas, plantas de progênie BR112014018560A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261591329P 2012-01-27 2012-01-27
PCT/US2013/022891 WO2013112686A1 (en) 2012-01-27 2013-01-24 Methods and compositions for generating complex trait loci

Publications (2)

Publication Number Publication Date
BR112014018560A2 BR112014018560A2 (pt) 2017-06-20
BR112014018560A8 true BR112014018560A8 (pt) 2017-07-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014018560A BR112014018560A8 (pt) 2012-01-27 2013-01-24 Planta ou semente, método para produzir uma segunda planta, método de produção de um locus, método de alteração de um locus, método de gerar uma biblioteca de plantas, método de identificar plantas, biblioteca de plantas, plantas de progênie

Country Status (10)

Country Link
US (4) US10435699B2 (pt)
EP (1) EP2807257A1 (pt)
CN (1) CN104245939A (pt)
AR (1) AR089793A1 (pt)
BR (1) BR112014018560A8 (pt)
CA (1) CA2862844A1 (pt)
EA (1) EA201491431A1 (pt)
MX (1) MX2014009023A (pt)
WO (1) WO2013112686A1 (pt)
ZA (1) ZA201405779B (pt)

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Also Published As

Publication number Publication date
CA2862844A1 (en) 2013-08-01
WO2013112686A1 (en) 2013-08-01
US20130198888A1 (en) 2013-08-01
US11788097B2 (en) 2023-10-17
CN104245939A (zh) 2014-12-24
US10435699B2 (en) 2019-10-08
US11312969B2 (en) 2022-04-26
BR112014018560A2 (pt) 2017-06-20
US20190376073A1 (en) 2019-12-12
EA201491431A1 (ru) 2015-01-30
AR089793A1 (es) 2014-09-17
US20220251586A1 (en) 2022-08-11
ZA201405779B (en) 2015-07-29
MX2014009023A (es) 2015-03-10
EP2807257A1 (en) 2014-12-03
US20240093214A1 (en) 2024-03-21

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