BR9814669A - Método para a integração de dna estranho emgenomas eucarióticos - Google Patents

Método para a integração de dna estranho emgenomas eucarióticos

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Publication number
BR9814669A
BR9814669A BR9814669-6A BR9814669A BR9814669A BR 9814669 A BR9814669 A BR 9814669A BR 9814669 A BR9814669 A BR 9814669A BR 9814669 A BR9814669 A BR 9814669A
Authority
BR
Brazil
Prior art keywords
methods
site
dna
target sites
compositions
Prior art date
Application number
BR9814669-6A
Other languages
English (en)
Inventor
Christopher L Baszczynski
Leszek Alexander Lyznik
William J Gordon-Kamm
Aragula Gururaj Rao
Laura A Tagliani
Xueni Guan
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of BR9814669A publication Critical patent/BR9814669A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

"MéTODO PARA A INTEGRAçãO DE DNA ESTRANHO EMGENOMAS EUCARIóTICOS". São fornecidos composições emétodos para introduzir um DNA de interesse em local-alvo de umgenoma. Em particular, os métodos e composições envolvem o usode uma combinação de locais-alvo para duas recombinasesespecíficas locais e expressão de uma recombinase quimérica comdupla especificidade de local-alvo. Dessa forma, a composiçãocompreende recombinases específicas locais originais comespecificidade para multiplicar locais-alvo, seq³ências denucleotídeos e cassetes de expressão codificando estasrecombinases ou locais-alvo. Os métodos envolvem atransformação de uma célula eucariótica tendo locais-alvo para arecombinase original com um DNA de interesse que é flanqueadopor locais-alvo correspondentes. A expressão da recombinaseresulta na integração do DNA de interesse no genoma da célula.As composições e métodos da invenção têm uso na construção decélulas eucarióticas transformadas de forma estável, e emparticular, células de plantas. Os métodos resultam na eficienteintegração de genoma objetivada do DNA por recombinaçãoespecífica local.
BR9814669-6A 1997-11-18 1998-11-17 Método para a integração de dna estranho emgenomas eucarióticos BR9814669A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US6561397P 1997-11-18 1997-11-18
US6562797P 1997-11-18 1997-11-18
US9943598P 1998-09-08 1998-09-08
PCT/US1998/024608 WO1999025840A1 (en) 1997-11-18 1998-11-17 A novel method for the integration of foreign dna into eukaryoticgenomes

Publications (1)

Publication Number Publication Date
BR9814669A true BR9814669A (pt) 2001-11-20

Family

ID=27370819

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9814669-6A BR9814669A (pt) 1997-11-18 1998-11-17 Método para a integração de dna estranho emgenomas eucarióticos

Country Status (12)

Country Link
US (3) US6262341B1 (pt)
EP (1) EP1032680B1 (pt)
AT (1) ATE317440T1 (pt)
AU (1) AU745960C (pt)
BR (1) BR9814669A (pt)
CA (1) CA2306188C (pt)
DE (1) DE69833457T2 (pt)
DK (1) DK1032680T3 (pt)
ES (1) ES2256972T3 (pt)
NZ (1) NZ504511A (pt)
PT (1) PT1032680E (pt)
WO (1) WO1999025840A1 (pt)

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