DE69833457D1 - Neuartige methode zur integration von fremd-dna ins eukaryotische genom - Google Patents

Neuartige methode zur integration von fremd-dna ins eukaryotische genom

Info

Publication number
DE69833457D1
DE69833457D1 DE69833457T DE69833457T DE69833457D1 DE 69833457 D1 DE69833457 D1 DE 69833457D1 DE 69833457 T DE69833457 T DE 69833457T DE 69833457 T DE69833457 T DE 69833457T DE 69833457 D1 DE69833457 D1 DE 69833457D1
Authority
DE
Germany
Prior art keywords
methods
site
dna
target sites
compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69833457T
Other languages
English (en)
Other versions
DE69833457T2 (de
Inventor
L Baszczynski
Alexander Lyznik
William J Gordon-Kamm
Gururaj Rao
A Tagliani
Xueni Guan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Application granted granted Critical
Publication of DE69833457D1 publication Critical patent/DE69833457D1/de
Publication of DE69833457T2 publication Critical patent/DE69833457T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69833457T 1997-11-18 1998-11-17 Neuartige methode zur integration von fremd-dna ins eukaryotische genom Expired - Lifetime DE69833457T2 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US6561397P 1997-11-18 1997-11-18
US6562797P 1997-11-18 1997-11-18
US65627P 1997-11-18
US65613P 1997-11-18
US9943598P 1998-09-08 1998-09-08
US99435P 1998-09-08
PCT/US1998/024608 WO1999025840A1 (en) 1997-11-18 1998-11-17 A novel method for the integration of foreign dna into eukaryoticgenomes

Publications (2)

Publication Number Publication Date
DE69833457D1 true DE69833457D1 (de) 2006-04-20
DE69833457T2 DE69833457T2 (de) 2006-09-14

Family

ID=27370819

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69833457T Expired - Lifetime DE69833457T2 (de) 1997-11-18 1998-11-17 Neuartige methode zur integration von fremd-dna ins eukaryotische genom

Country Status (12)

Country Link
US (3) US6262341B1 (de)
EP (1) EP1032680B1 (de)
AT (1) ATE317440T1 (de)
AU (1) AU745960C (de)
BR (1) BR9814669A (de)
CA (1) CA2306188C (de)
DE (1) DE69833457T2 (de)
DK (1) DK1032680T3 (de)
ES (1) ES2256972T3 (de)
NZ (1) NZ504511A (de)
PT (1) PT1032680E (de)
WO (1) WO1999025840A1 (de)

Families Citing this family (235)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US7670823B1 (en) 1999-03-02 2010-03-02 Life Technologies Corp. Compositions for use in recombinational cloning of nucleic acids
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US7119250B2 (en) * 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
JP4303418B2 (ja) * 1997-10-24 2009-07-29 ライフ テクノロジーズ コーポレーション 組換え部位を有する核酸を使用する組換えクローニング
DE69841438D1 (de) * 1997-11-18 2010-02-25 Pioneer Hi Bred Int Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
US6187994B1 (en) * 1997-11-18 2001-02-13 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
US7102055B1 (en) 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
WO1999055851A2 (en) * 1998-04-28 1999-11-04 Novartis Ag Site-directed transformation of plants
US6746870B1 (en) 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
EP1978098A3 (de) * 1999-12-10 2009-02-18 Invitrogen Corporation Verwendung von mehrfachen Rekombinationsstellen mit einzigartiger Spezifizität beim Rekombinationsklonen
DE60034808T2 (de) 1999-12-16 2008-01-31 Cropdesign N.V. Optimierter t-dna transfer und entsprechende vektoren
US20020023278A1 (en) * 2000-05-08 2002-02-21 Lyznik Leszek Alexander Genetic transformation in plants using site-specific recombination and wide hybridization
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
MXPA03000634A (es) * 2000-07-21 2004-12-03 Us Agriculture Metodos para el reemplazo, el desplazamiento y acumulacion de adn en genomas eucarioticos.
AU2001278066A1 (en) * 2000-07-28 2002-02-13 University Of Connecticut Methods for the controlled, automatic excision of heterologous DNA from transgenic plants and DNA-excising gene cassettes for use therein
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7560622B2 (en) * 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
WO2002086144A2 (en) * 2001-04-19 2002-10-31 Invitrogen Corporation Compositions and methods for recombinational cloning of nucleic acid molecules
WO2002095055A2 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
JP4489424B2 (ja) * 2001-05-30 2010-06-23 グラクソ グループ リミテッド 染色体に基くプラットホーム
JP4771656B2 (ja) * 2001-05-30 2011-09-14 カリックス・バイオ−ベンチャーズ・インコーポレイテッド 植物人工染色体、その使用及び植物人工染色体の製造方法
AU2002355155A1 (en) * 2001-07-24 2003-02-17 Affinium Pharmaceuticals Inc. Methods for gene disruption and uses thereof
AU2003220642C1 (en) 2002-03-29 2009-01-29 Syngenta Participations Ag Lambda integrase mediated recombination in plants
US7164056B2 (en) * 2002-05-03 2007-01-16 Pioneer Hi-Bred International, Inc. Gene targeting using replicating DNA molecules
US8304233B2 (en) 2002-06-04 2012-11-06 Poetic Genetics, Llc Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
US7476777B2 (en) * 2002-09-17 2009-01-13 Ceres, Inc. Biological containment system
EP1405908A1 (de) * 2002-10-04 2004-04-07 ProBioGen AG Herstellung von Zelllinien zur heterologen Expression
US20040137624A1 (en) * 2002-12-27 2004-07-15 Lowe Brenda A. Methods of site-directed transformation
EP2535414B1 (de) 2003-04-29 2017-12-13 Pioneer Hi-Bred International Inc. Neue Glyphosat-N-Acetyltransferase-Gene (GAT)
WO2005028615A2 (en) * 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US7935862B2 (en) * 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
WO2005059148A1 (en) * 2003-12-17 2005-06-30 Pioneer Hi-Bred International, Inc. Recombinase mediated gene traps
AU2005327983B2 (en) 2004-06-30 2008-08-07 Pioneer Hi-Bred International, Inc. Methods of protecting plants from pathogenic fungi
BR122015026853B1 (pt) 2004-07-02 2017-03-28 Du Pont cassete de expressão, microorganismo transformado, método para indução de resistência a patógeno de planta em uma planta, composição anti-patogênica e método para proteção de uma planta contra um patógeno de planta
US7514237B2 (en) * 2004-09-02 2009-04-07 Wyeth Systems and methods for protein production
US7736897B2 (en) * 2005-07-18 2010-06-15 Pioneer Hi-Bred International, Inc. FRT recombination sites and methods of use
JP2009515522A (ja) 2005-11-10 2009-04-16 パイオニア ハイ−ブレッド インターナショナル， インコーポレイテッド Ｄｏｆ（ｄｎａｂｉｎｄｉｎｇｗｉｔｈｏｎｅｆｉｎｇｅｒ）配列および使用方法
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
US8058509B2 (en) * 2005-12-21 2011-11-15 Pioneer Hi-Bred International, Inc. Methods and compositions for in planta production of inverted repeats
US7981677B2 (en) 2006-03-01 2011-07-19 Pioneer Hi-Bred International, Inc. Identifying a high oil phenotype in maize by detecting a marker locus genetically linked with a QTL6 region
BRPI0710455A2 (pt) 2006-04-19 2011-08-16 Pioneer Hi Bred Int polipeptìdeo isolado, polinucleotìdeo isolado e métodos para aumentar o nìvel de um polipeptìdeo em uma planta, para modular o nìvel de um polipeptìdeo em uma planta e para modificar o crescimento de uma planta
WO2007134122A2 (en) 2006-05-09 2007-11-22 The Curators Of The University Of Missouri Plant artificial chromosome platforms via telomere truncation
CA2652461C (en) 2006-05-16 2015-12-01 Pioneer Hi-Bred International, Inc. Antifungal polypeptides and uses thereof in inducing fungal resistance in plants
CN101490267B (zh) 2006-05-17 2013-04-17 先锋高级育种国际公司 人工植物微染色体
US7951995B2 (en) 2006-06-28 2011-05-31 Pioneer Hi-Bred International, Inc. Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof
US9206397B2 (en) * 2006-07-07 2015-12-08 The Fred Hutchinson Cancer Research Center High efficiency FLP site-specific recombination in mammalian cells using an optimized FLP gene
EP1942192A1 (de) * 2007-01-08 2008-07-09 Heinrich-Pette-Institut für experimentelle Virologie und Immunologie Verwendung einer massgeschneiderten Recombinase für die Behandlung einer retroviralen Erkrankung
AU2008212127B2 (en) 2007-02-05 2013-03-28 National University Of Singapore Putative cytokinin receptor and methods for use thereof
WO2008145629A2 (en) 2007-05-25 2008-12-04 Cropdesign N.V. Yield enhancement in plants by modulation of maize alfins
BRPI0816750A2 (pt) 2007-06-29 2015-09-29 Pioneer Hi Bred Int métodos para alterar o genoma de uma célula de planta monocotiledônea e para modificar uma sequência alvo genômica endógena específica e planta de milho
EP2031067A1 (de) * 2007-08-30 2009-03-04 Albert-Ludwigs-Universität Freiburg Herstellung von genetisch modifizierten Aktinomyceten durch Rekombination
US8847013B2 (en) 2008-01-17 2014-09-30 Pioneer Hi Bred International Inc Compositions and methods for the suppression of target polynucleotides from lepidoptera
US8367895B2 (en) 2008-01-17 2013-02-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from the family aphididae
AR070495A1 (es) 2008-02-29 2010-04-07 Monsanto Technology Llc Evento de maiz mon87460 y composiciones y metodos para detectarlo
US20110107445A1 (en) * 2008-03-26 2011-05-05 Iti Scotland Limited Efficient Insertion of DNA Into Embryonic Stem Cells
AP2011005671A0 (en) 2008-09-26 2011-04-30 Basf Agrochemical Products Bv Herbicide-resistant AHAS-mutants and methods of use.
WO2010065867A1 (en) 2008-12-04 2010-06-10 Pioneer Hi-Bred International, Inc. Methods and compositions for enhanced yield by targeted expression of knotted1
US8293533B2 (en) 2008-12-19 2012-10-23 E.I. Du Pont De Nemours And Company Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
CA2749524C (en) 2009-01-22 2021-07-06 Syngenta Participations Ag Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
US9347046B2 (en) 2009-01-22 2016-05-24 Syngenta Participations Ag Hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
GB2467167B (en) * 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
US20100210460A1 (en) 2009-02-19 2010-08-19 Pioneer Hi-Bred International, Inc. Blended refuge deployment via manipulation during hybrid seed production
MX2011011678A (es) 2009-05-04 2011-12-06 Pioneer Hi Bred Int Mejora de la produccion en plantas mediante la modulacion del factor de transcripcion ap2.
MX2011013224A (es) 2009-06-09 2012-06-01 Pioneer Hi Bred Int Promotor de endospermo temprano y metodos de uso.
AU2010286111A1 (en) 2009-08-21 2012-02-16 Beeologics Inc. Preventing and curing beneficial insect diseases via plant transcribed molecules
BR122018067925B8 (pt) 2009-08-28 2022-12-06 Du Pont Polinucleotídeo isolado, cassete de expressão, método para controlar uma praga de plantas do tipo coleoptera e método para obtenção de uma planta
WO2011056544A1 (en) 2009-10-26 2011-05-12 Pioneer Hi-Bred International, Inc. Somatic ovule specific promoter and methods of use
EP3078748B1 (de) 2009-12-30 2021-08-11 Pioneer Hi-Bred International, Inc. Verfahren und zusammensetzungen zur einbringung und regulierten expression von genen in pflanzen
WO2011082310A2 (en) 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted polynucleotide modification
EP2529028A1 (de) 2010-01-26 2012-12-05 Pioneer Hi-Bred International, Inc. Mit herbizidtoleranz assoziierte polynukleotid- und polypeptidsequenzen
EP2560473A1 (de) 2010-04-23 2013-02-27 Pioneer Hi-Bred International Inc. Genschalterzusammensetzungen und verfahren zu ihrer verwendung
MX2012012672A (es) 2010-05-06 2012-12-17 Du Pont Gen y proteina acc sintasa 3 de maiz y sus usos.
RU2617968C2 (ru) * 2010-05-27 2017-04-28 Хайнрих-Петте-Институт, Ляйбниц-Институт Фюр Экспериментелле Фирологи-Штифтунг Бюргерлихен Рехтс Адаптированная рекомбиназа для рекомбинации асимметричных участков-мишеней во множестве штаммов ретровирусов
MX365030B (es) 2010-06-25 2019-05-21 Du Pont Composiciones y metodos para mejorar la resistencia al tizon norteño de las hojas en maiz.
EP2603591A1 (de) 2010-08-13 2013-06-19 Pioneer Hi-Bred International Inc. Zusammensetzungen und verfahren mit sequenzen mit hydroxyphenylpyruvat-dioxygenase (hppd)-aktivität
US8865967B2 (en) 2010-08-23 2014-10-21 Pioneer Hi Bred International Inc Defensin variants and methods of use
CN103748227A (zh) 2010-11-24 2014-04-23 先锋国际良种公司 芸苔gat事件dp-073496-4及其鉴定和/或检测组合物和方法
CA2818918A1 (en) 2010-11-24 2012-05-31 Pioneer Hi-Bred International, Inc. Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof
AR083029A1 (es) 2010-12-09 2013-01-23 Syngenta Participations Ag Metodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
WO2012092106A1 (en) 2010-12-28 2012-07-05 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with lepidopteran activity
US9109231B2 (en) 2011-02-11 2015-08-18 Pioneer Hi Bred International Inc Synthetic insecticidal proteins active against corn rootworm
WO2012112411A1 (en) 2011-02-15 2012-08-23 Pioneer Hi-Bred International, Inc. Root-preferred promoter and methods of use
US8878007B2 (en) 2011-03-10 2014-11-04 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with lepidopteran activity
AR085533A1 (es) 2011-03-23 2013-10-09 Pioneer Hi Bred Int Metodos de produccion de un locus de rasgos transgenicos complejo
WO2012131495A2 (en) 2011-03-30 2012-10-04 Universidad Nacional Autónoma de México Mutant bacillus thuringiensis cry genes and methods of use
US20120266324A1 (en) 2011-04-15 2012-10-18 Pioneer Hi Bred International Inc. Self-Reproducing Hybrid Plants
EP2702160B1 (de) 2011-04-27 2020-05-27 Amyris, Inc. Verfahren zur genommodifikation
US9062317B2 (en) 2011-05-09 2015-06-23 E I Du Pont De Nemours And Company Methods and compositions for silencing gene families using artificial microRNAs
US9150625B2 (en) 2011-05-23 2015-10-06 E I Du Pont De Nemours And Company Chloroplast transit peptides and methods of their use
AU2012333207A1 (en) 2011-06-21 2014-01-23 E.I. Du Pont De Nemours And Company Methods and compositions for producing male sterile plants
EP2739739A1 (de) 2011-08-03 2014-06-11 E. I. Du Pont de Nemours and Company Verfahren und zusammensetzungen zur gezielten einführung in eine pflanze
AU2012301912A1 (en) 2011-08-31 2014-03-06 E. I. Dupont De Nemours & Company Methods for tissue culture and transformation of sugarcane
IN2014DN02042A (de) 2011-10-28 2015-05-15 Du Pont
US20140298544A1 (en) 2011-10-28 2014-10-02 Pioneer Hi Bred International Inc Engineered PEP carboxylase variants for improved plant productivity
US9006515B2 (en) 2012-01-06 2015-04-14 Pioneer Hi Bred International Inc Pollen preferred promoters and methods of use
WO2013103371A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Ovule specific promoter and methods of use
EP2800814A1 (de) 2012-01-06 2014-11-12 Pioneer Hi-Bred International Inc. Verfahren zum screening von pflanzen auf genetische elemente zur induktion von parthenogenese in pflanzen
EP2802215B1 (de) 2012-01-11 2021-09-15 The Australian National University Verfahren zur modulation der pflanzenwurzelarchitektur
AR089793A1 (es) 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
EP2814965B1 (de) 2012-02-16 2018-03-21 Syngenta Participations AG Manipulierte pestizidproteine
WO2013134499A1 (en) 2012-03-07 2013-09-12 The Johns Hopkins University Anastasis biosensor
ES2828663T3 (es) 2012-04-18 2021-05-27 Univ Leland Stanford Junior Dirección genética no disruptiva
BR112014027468A2 (pt) 2012-05-04 2017-06-27 Du Pont polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.
CA2876426A1 (en) 2012-06-15 2013-12-19 E. I. Du Pont De Nemours And Company Methods and compositions involving als variants with native substrate preference
US9347105B2 (en) 2012-06-15 2016-05-24 Pioneer Hi Bred International Inc Genetic loci associated with resistance of soybean to cyst nematode and methods of use
EP2864484A1 (de) 2012-06-22 2015-04-29 Syngenta Participations AG Biologische bekämpfung von koleopteren
US9816102B2 (en) 2012-09-13 2017-11-14 Indiana University Research And Technology Corporation Compositions and systems for conferring disease resistance in plants and methods of use thereof
US20140109259A1 (en) 2012-10-11 2014-04-17 Pioneer Hi Bred International Inc Guard Cell Promoters and Uses Thereof
CN104884625A (zh) 2012-10-15 2015-09-02 先锋国际良种公司 增强cry内毒素的活性的方法和组合物
US20140173775A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic plants
US20150351390A1 (en) 2012-12-21 2015-12-10 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
WO2014116989A1 (en) 2013-01-25 2014-07-31 Pioneer Hi-Bred International, Inc. Maize event dp-032218-9 and methods for detection thereof
BR112015022742A2 (pt) 2013-03-11 2018-11-27 Pionner Hi Bred Int Inc métodos e composições que empregam um domínio de estabilização dependente de sulfonilureia
CA2905377A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Methods and compositions to improve the spread of chemical signals in plants
US9273322B2 (en) 2013-03-12 2016-03-01 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
UA123532C2 (uk) 2013-03-12 2021-04-21 Е. І. Дю Пон Де Немур Енд Компані Спосіб ідентифікації варіантного сайта розпізнавання для сконструйованого засобу, що рідко розщеплює, для індукції двониткового розриву
US9243258B2 (en) 2013-03-12 2016-01-26 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
US9803214B2 (en) 2013-03-12 2017-10-31 Pioneer Hi-Bred International, Inc. Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility
CA2905743C (en) 2013-03-13 2021-09-28 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in brassica
BR112015023268A2 (pt) 2013-03-13 2018-05-02 Du Pont método para reduzir a expressão, construto de dna, método de obtenção de célula vegetal, método de obtenção de planta transgênica ou uma parte de planta
BR112015023272A2 (pt) 2013-03-14 2017-07-18 Pioneer Hi Bred Int célula vegetal, planta, explante vegetal, semente transgênica, método para produzir uma célula vegetal tendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade de dicamba descarboxilase, método para controlar plantas daninhas em um campo contendo uma cultura e método para controlar plantas daninhas em um campo contendo uma cultura
CN105339380A (zh) 2013-03-14 2016-02-17 先锋国际良种公司 用以防治昆虫害虫的组合物和方法
US20160040149A1 (en) 2013-03-14 2016-02-11 Pioneer Hi-Bred International Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
EP2971000A4 (de) 2013-03-15 2016-11-23 Pioneer Hi Bred Int Phi-4-polypeptide und verfahren zu deren verwendung
US11013917B2 (en) 2013-06-21 2021-05-25 Newsouth Innovations Pty Limited Method and apparatus for close-field electroporation
US11459579B2 (en) 2013-07-09 2022-10-04 Board Of Trustees Of Michigan State University Transgenic plants produced with a K-domain, and methods and expression cassettes related thereto
US10570409B2 (en) 2013-07-09 2020-02-25 Board Of Trustees Of Michigan State University Transgenic plants produced with a K-domain, and methods and expression cassettes related thereto
BR112016001918A2 (pt) 2013-07-31 2017-08-29 Du Pont Semente de soja transgênica, farelo, progênie, produtos e subprodutos, métodos para aumentar ácidos graxos totais e a percentagem de ácidos graxos totais, terceira planta transgênica e alimento ou ração
WO2015021139A2 (en) 2013-08-08 2015-02-12 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides having broad spectrum activity and uses thereof
WO2015023846A2 (en) 2013-08-16 2015-02-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2015038622A1 (en) 2013-09-11 2015-03-19 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
EP4159028A1 (de) 2013-09-13 2023-04-05 Pioneer Hi-Bred International, Inc. Insektizidproteine und verfahren zu deren verwendung
BR112016008172B1 (pt) 2013-10-16 2021-06-15 The Australian National University Método para modulação do crescimento de plantas
WO2015057600A1 (en) 2013-10-18 2015-04-23 E. I. Du Pont De Nemours And Company Glyphosate-n-acetyltransferase (glyat) sequences and methods of use
CA2928830A1 (en) 2013-10-29 2015-05-07 Shai J. Lawit Self-reproducing hybrid plants
PT3083958T (pt) 2013-12-19 2019-06-27 Amyris Inc Métodos de integração genómica
BR112016018103B1 (pt) 2014-02-07 2024-01-16 E.I. Du Pont De Nemours And Company Polipeptídeo e seu uso, polinucleotídeo, composição, proteína de fusão, método para controlar uma população, método para inibir o crescimento, método para controlar a infestação, método para obtenção de uma planta ou célula vegetal, construto
MX2016010187A (es) 2014-02-07 2017-07-11 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
MX2016011690A (es) 2014-03-21 2016-11-07 Univ Leland Stanford Junior Edicion del genoma sin nucleasas.
AR100159A1 (es) 2014-04-22 2016-09-14 Du Pont Genes de anhidrasa carbónica plastídica para el incremento de aceite en semillas con expresión incrementada de dgat
WO2016000237A1 (en) 2014-07-03 2016-01-07 Pioneer Overseas Corporation Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes
WO2016022516A1 (en) 2014-08-08 2016-02-11 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
EP2993229A1 (de) 2014-09-02 2016-03-09 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Verträgliche und hochspezifische massgeschneiderte Rekombinase zur Rekombination asymmetrischer Zielorte in mehreren Retrovirenstämmen
AU2015315651A1 (en) 2014-09-12 2017-02-16 E. I. Du Pont De Nemours And Company Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use
WO2016044092A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
CA2963550A1 (en) 2014-10-16 2016-04-21 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides having broad spectrum activity and uses thereof
CA2963558C (en) 2014-10-16 2023-04-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX2017004814A (es) 2014-10-16 2017-08-02 Pioneer Hi Bred Int Polipeptidos insecticidas que tienen un espectro de actividad mejorado y sus usos.
MX2017007619A (es) 2014-12-12 2017-09-18 Syngenta Participations Ag Composiciones y metodos para controlar plagas en plantas.
US20170327834A1 (en) 2014-12-15 2017-11-16 Syngenta Participations Ag Pesticidal microrna carriers and use thereof
WO2016100309A1 (en) 2014-12-16 2016-06-23 Pioneer Hi-Bred International, Inc. Restoration of male fertility in wheat
US20170359965A1 (en) 2014-12-19 2017-12-21 E I Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
BR112017013528A2 (pt) 2014-12-23 2018-03-06 Syngenta Participations Ag controle biológico de pragas de coleópteros
MX2017011525A (es) 2015-03-11 2018-01-30 Pioneer Hi Bred Int Combinaciones insecticidas de pip-72 y metodos de uso.
BR112017017279A2 (pt) 2015-03-19 2018-04-17 Pioneer Hi Bred Int métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta
EP3091076A1 (de) 2015-05-07 2016-11-09 Limagrain Europe Für die haploide induktion in maispflanzen verantwortliches polynukleotid und zugehörige verfahren
RU2017144238A (ru) 2015-05-19 2019-06-19 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
US10647995B2 (en) 2015-06-16 2020-05-12 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
RU2762832C2 (ru) 2015-08-06 2021-12-23 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки растительного происхождения и способы их применения
CN108513584A (zh) 2015-08-28 2018-09-07 先锋国际良种公司 苍白杆菌介导的植物转化
WO2021257206A1 (en) 2020-06-17 2021-12-23 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
EA037614B1 (ru) 2015-10-16 2021-04-21 Пайонир Хай-Бред Интернэшнл, Инк. Получение растений маиса с повышенной устойчивостью к северной пятнистости листьев
WO2017079026A1 (en) 2015-11-06 2017-05-11 E. I. Du Pont De Nemours And Company Generation of complex trait loci in soybean and methods of use
BR112018012887B1 (pt) 2015-12-22 2024-02-06 Pioneer Hi-Bred International, Inc Cassete de expressão, vetor, métodos de obtenção de célula vegetal e planta transgênica, métodos para expressar um polinucleotídeo
US20190098858A1 (en) 2016-03-18 2019-04-04 Pioneer Hi-Bred International, Inc. Methods and compositions for producing clonal, non-reduced, non-recombined gametes
RU2018137045A (ru) 2016-04-14 2020-05-14 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные полипептиды, обладающие улучшенным спектром активности, и пути их применения
AR108284A1 (es) 2016-04-19 2018-08-08 Pioneer Hi Bred Int Combinaciones insecticidas de polipéptidos que tienen espectro de actividad mejorado y usos de éstas
EP3451837B1 (de) 2016-05-04 2021-08-25 Pioneer Hi-Bred International, Inc. Insektizide proteine und verfahren zu deren verwendung
JP7093310B2 (ja) 2016-05-18 2022-06-29 アミリス， インコーポレイテッド 外因性ランディングパッドへの核酸のゲノム組込みのための組成物および方法
BR112018076047A2 (pt) 2016-06-16 2019-03-26 Pioneer Hi Bred Int elemento de silenciamento, construto de dna, cassete de expressão, célula hospedeira, composição, célula vegetal, planta ou parte de planta, semente transgênica, método para controlar um inseto-praga de planta e kit
ES2924552T3 (es) 2016-06-24 2022-10-07 Pioneer Hi Bred Int Elementos reguladores de las plantas y métodos de uso de los mismos
MX2018015906A (es) 2016-07-01 2019-04-04 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
AR109206A1 (es) 2016-08-05 2018-11-07 Syngenta Participations Ag Control de plagas de coleópteros utilizando moléculas de arn
AR109205A1 (es) 2016-08-05 2018-11-07 Syngenta Participations Ag Control de plagas de coleópteros utilizando moléculas de arn
WO2018076335A1 (en) 2016-10-31 2018-05-03 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Compositions and methods for enhancing abiotic stress tolerance
US11021716B2 (en) 2016-11-01 2021-06-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018094020A1 (en) 2016-11-16 2018-05-24 The Johns Hopkins University Anastasis biosensor
US11174295B2 (en) 2016-12-14 2021-11-16 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3558004A1 (de) 2016-12-22 2019-10-30 Pioneer Hi-Bred International, Inc. Insektizide proteine und verfahren zu deren verwendung
CA3052794A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
EP3622076A1 (de) 2017-05-11 2020-03-18 Pioneer Hi-Bred International, Inc. Insektizide proteine und verfahren zu deren verwendung
US20200308598A1 (en) 2017-05-26 2020-10-01 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides having improved activity spectrum and uses thereof
EP3638776A1 (de) 2017-06-14 2020-04-22 Technische Universität Dresden Verfahren und mittel zur genetischen veränderung von genomen unter verwendung von designer-dna-rekombinatiosenzymen
US11649465B2 (en) 2017-09-11 2023-05-16 R.J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
CA3076831A1 (en) 2017-09-25 2019-03-28 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
WO2019074598A1 (en) 2017-10-13 2019-04-18 Pioneer Hi-Bred International, Inc. VIRUS-INDUCED GENETIC SILENCING TECHNOLOGY FOR THE CONTROL OF INSECTS IN MAIZE
AR113761A1 (es) 2017-10-18 2020-06-10 Syngenta Participations Ag Control de plagas de hemípteros utilizando moléculas de arn
WO2019099769A1 (en) 2017-11-16 2019-05-23 The Johns Hopkins University Anastasis biosensor caspase tracker
US11492639B2 (en) 2017-12-19 2022-11-08 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides and uses thereof
BR112020016306A2 (pt) 2018-02-12 2020-12-15 Curators Of The University Of Missouri Gene (saur) suprarregulado pequeno de auxina para o melhoramento da arquitetura do sistema radicular da planta, tolerância ao encharcamento, resistência à seca, e rendimento
CA3091431A1 (en) 2018-02-26 2019-08-29 Devgen Nv Control of insect pests using rna molecules
EP3759489A1 (de) 2018-03-02 2021-01-06 Pioneer Hi-Bred International, Inc. Pflanzengesundheitstest
CA3092075A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CA3092078A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US20210123069A1 (en) 2018-04-27 2021-04-29 Devgen Nv Control of insect pests using rna molecules
US11702668B2 (en) 2018-05-22 2023-07-18 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
EP3833747A1 (de) 2018-06-28 2021-06-16 Pioneer Hi-Bred International, Inc. Verfahren zur erzeugung transformierter pflanzen
WO2020046701A1 (en) 2018-08-29 2020-03-05 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3874050A1 (de) 2018-10-31 2021-09-08 Pioneer Hi-Bred International, Inc. Zusammensetzungen und verfahren zur ochrobacter vermittelten pflanzentransformation
EP3938521A1 (de) 2019-03-11 2022-01-19 Pioneer Hi-Bred International, Inc. Verfahren zur klonalen pflanzenproduktion
CA3129553A1 (en) 2019-03-21 2020-09-24 Lien DE SCHRIJVER Control of insect pests using rna molecules
WO2020198408A1 (en) 2019-03-27 2020-10-01 Pioneer Hi-Bred International, Inc. Plant explant transformation
WO2020198496A1 (en) 2019-03-28 2020-10-01 Pioneer Hi-Bred International, Inc. Modified agrobacterium strains and use thereof for plant transformation
WO2020214986A1 (en) 2019-04-18 2020-10-22 Pioneer Hi-Bred International, Inc. Embryogenesis factors for cellular reprogramming of a plant cell
EP3831939A1 (de) * 2019-12-06 2021-06-09 Technische Universität Dresden Fusion von stellenspezifischen rekombinasen zur effizienten und spezifischen genom-editierung
TW202142114A (zh) 2020-02-04 2021-11-16 美商陶氏農業科學公司 具有殺有害生物效用之組成物及與其相關之方法
US20230172175A1 (en) 2020-03-15 2023-06-08 Proteinea, Inc. Recombinant protein production in insects
CA3175936A1 (en) 2020-06-12 2021-12-16 Pioneer Hi-Bred International, Inc. Alteration of seed composition in plants
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
EP4192848A1 (de) 2020-08-10 2023-06-14 Pioneer Hi-Bred International, Inc. Pflanzenregulierungselemente und verfahren zur verwendung davon
BR112023002602A2 (pt) 2020-08-10 2023-04-04 Du Pont Composições e métodos para aumentar a resistência a helmintosporiose no milho
US20240002870A1 (en) 2020-09-30 2024-01-04 Pioneer Hi-Bred International, Inc. Rapid transformation of monocot leaf explants
EP4232586A1 (de) 2020-10-21 2023-08-30 Pioneer Hi-Bred International, Inc. Verdoppelter haploider induktor
CA3197681A1 (en) 2020-10-21 2022-04-28 Pioneer Hi-Bred International, Inc. Parthenogenesis factors and methods of using same
EP4251755A2 (de) 2020-11-24 2023-10-04 AgBiome, Inc. Pestizidgene und verfahren zur verwendung
US20240141311A1 (en) 2021-04-22 2024-05-02 North Carolina State University Compositions and methods for generating male sterile plants
EP4334457A1 (de) 2021-05-06 2024-03-13 Agbiome, Inc. Pestizidgene und verfahren zur verwendung
US20220386574A1 (en) * 2021-06-03 2022-12-08 Mayo Foundation For Medical Education And Research P21-cre animal models
WO2023076898A1 (en) 2021-10-25 2023-05-04 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
WO2023107943A1 (en) 2021-12-07 2023-06-15 AgBiome, Inc. Pesticidal genes and methods of use
TW202345696A (zh) 2022-05-18 2023-12-01 美商科迪華農業科技有限責任公司 具有殺有害生物效用之組成物及與其相關的方法
WO2024023578A1 (en) 2022-07-28 2024-02-01 Institut Pasteur Hsc70-4 in host-induced and spray-induced gene silencing
WO2024044596A1 (en) 2022-08-23 2024-02-29 AgBiome, Inc. Pesticidal genes and methods of use
EP4360451A1 (de) 2022-10-25 2024-05-01 Limagrain Europe Mutanten zur haploiden induktion
WO2024129674A1 (en) 2022-12-13 2024-06-20 AgBiome, Inc. Pesticidal genes and methods of use

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
JP3720353B2 (ja) 1992-12-04 2005-11-24 メディカル リサーチ カウンシル 多価および多重特異性の結合タンパク質、それらの製造および使用
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
EP0632054A1 (de) 1993-06-28 1995-01-04 European Molecular Biology Laboratory Regulation stellenspezifischer Rekombination mittels stellungsspezifischer Rekombinase/Kernrezeptor-Fusionsproteine
CA2177367A1 (en) 1993-12-03 1995-06-08 Andrew David Griffiths Recombinant binding proteins and peptides
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
EP1227147A3 (de) 1995-06-07 2002-08-14 Invitrogen Corporation Rekombinatorische Klonierung unter Verwendung genmanipulierter Rekombinationsorte
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
AUPN523995A0 (en) 1995-09-05 1995-09-28 Crc For Biopharmaceutical Research Pty Ltd Method for producing phage display vectors
EP0876094A4 (de) 1995-10-13 2002-04-24 Purdue Research Foundation Verfahren zur herstellung von hybridpflanzen
AUPN903196A0 (en) 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US6774279B2 (en) * 1997-05-30 2004-08-10 Carnegie Institution Of Washington Use of FLP recombinase in mice
US6114600A (en) 1997-10-31 2000-09-05 The United States Of America As Represented By The Secretary Of Agriculture Resolution of complex integration patterns to obtain single copy transgenes
US6187994B1 (en) 1997-11-18 2001-02-13 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
DE69841438D1 (de) 1997-11-18 2010-02-25 Pioneer Hi Bred Int Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
WO1999055851A2 (en) 1998-04-28 1999-11-04 Novartis Ag Site-directed transformation of plants

Also Published As

Publication number Publication date
DE69833457T2 (de) 2006-09-14
AU745960B2 (en) 2002-04-11
CA2306188C (en) 2008-08-05
US6541231B1 (en) 2003-04-01
DK1032680T3 (da) 2006-05-22
ES2256972T3 (es) 2006-07-16
US6262341B1 (en) 2001-07-17
AU745960C (en) 2003-09-18
PT1032680E (pt) 2006-05-31
EP1032680B1 (de) 2006-02-08
BR9814669A (pt) 2001-11-20
EP1032680A1 (de) 2000-09-06
US20030119166A1 (en) 2003-06-26
ATE317440T1 (de) 2006-02-15
CA2306188A1 (en) 1999-05-27
WO1999025840A1 (en) 1999-05-27
AU1590499A (en) 1999-06-07
NZ504511A (en) 2002-12-20
US7223601B2 (en) 2007-05-29

Similar Documents

Publication Publication Date Title
DE69833457D1 (de) Neuartige methode zur integration von fremd-dna ins eukaryotische genom
ES2784754T3 (es) Métodos y composiciones para modificar un locus objetivo
Shayevitch et al. The importance of DNA methylation of exons on alternative splicing
KR102098915B1 (ko) 키메라 게놈 조작 분자 및 방법
ES2666179T3 (es) Montaje de ADN mediado por nucleasas
Ren et al. A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice
KR20190019931A (ko) 다중 가이드 RNAs를 이용한 면역학적 내성 파괴 방법
WO2019103442A3 (ko) CRISPR/Cpf1 시스템을 이용한 유전체 편집용 조성물 및 이의 용도
DK0937098T3 (da) Rekombinatorisk kloning under anvendelse af gensplejsede rekombinationssites
KR102151065B1 (ko) 동물 배아의 염기 교정용 조성물 및 염기 교정 방법
Ma et al. An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites
DK0973928T3 (da) DNA-baseret transposonsystem til indføring af nukleinsyre i DNA i en celle
DE60208014D1 (de) Verfahren zur herstellung einer stabilen inversion einer dna-sequenz durch ortsspezifische rekombination und dna-vektoren und transgene zellen davon
Yu et al. A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice
Wefers et al. Generation of targeted mouse mutants by embryo microinjection of TALENs
KR102679001B1 (ko) 신규의 개량된 염기 편집 또는 교정용 융합단백질 및 이의 용도
EP4284923A1 (de) Crispr-assoziierte baseneditierung des komplementären strangs
KR20210148089A (ko) 표적화된 변형의 표적화 벡터로의 무흔적 도입을 위한 방법
Yin et al. PCR-based procedures to isolate insertion sites of DNA elements
Jamsai et al. Insertion of modifications in the β-globin locus using GET recombination with single-stranded oligonucleotides and denatured PCR fragments
WO2004005474A3 (en) A robust, inducible cardiac preferred expression system for transgenesis
Dujon Homing endonucleases and the yeast mitochondrial ω locus—a historical perspective
Ansai et al. Targeted mutagenesis using CRISPR/Cas system
EP3650557B1 (de) Verfahren zur bestimmung der restriktionseffizienz von endonukleasen, die doppelstrangbrüche in dna-zielsequenzen vermitteln
WO2003054217A3 (en) Plant msh2 sequences and methods of use

Legal Events

Date Code Title Description
8364 No opposition during term of opposition