PT3083958T - Métodos de integração genómica - Google Patents
Métodos de integração genómicaInfo
- Publication number
- PT3083958T PT3083958T PT14831130T PT14831130T PT3083958T PT 3083958 T PT3083958 T PT 3083958T PT 14831130 T PT14831130 T PT 14831130T PT 14831130 T PT14831130 T PT 14831130T PT 3083958 T PT3083958 T PT 3083958T
- Authority
- PT
- Portugal
- Prior art keywords
- methods
- genomic integration
- genomic
- integration
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361918625P | 2013-12-19 | 2013-12-19 | |
| US201461937444P | 2014-02-07 | 2014-02-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT3083958T true PT3083958T (pt) | 2019-06-27 |
Family
ID=52432914
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT14831130T PT3083958T (pt) | 2013-12-19 | 2014-12-19 | Métodos de integração genómica |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US9476065B2 (pt) |
| EP (1) | EP3083958B1 (pt) |
| JP (1) | JP6652489B2 (pt) |
| KR (1) | KR102274445B1 (pt) |
| CN (1) | CN106029886B (pt) |
| AU (1) | AU2014368982B2 (pt) |
| CA (1) | CA2933902C (pt) |
| MX (1) | MX2016007797A (pt) |
| PT (1) | PT3083958T (pt) |
| WO (1) | WO2015095804A1 (pt) |
Families Citing this family (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| PT3456831T (pt) | 2013-04-16 | 2021-09-10 | Regeneron Pharma | Modificação alvejada do genoma de rato |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| AU2014346559B2 (en) | 2013-11-07 | 2020-07-09 | Editas Medicine,Inc. | CRISPR-related methods and compositions with governing gRNAs |
| KR102170502B1 (ko) | 2013-12-11 | 2020-10-28 | 리제너론 파마슈티칼스 인코포레이티드 | 게놈의 표적화된 변형을 위한 방법 및 조성물 |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| JP2017518075A (ja) | 2014-05-30 | 2017-07-06 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | 潜伏性ウイルス感染を処置するための組成物および方法 |
| BR112016030145A8 (pt) | 2014-06-23 | 2018-12-11 | Regeneron Pharma | métodos para a montagem de ao menos dois ácidos nucleicos e de dois ou mais ácidos nucleicos |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| CA2963820A1 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| PT3221457T (pt) | 2014-11-21 | 2019-06-27 | Regeneron Pharma | Métodos e composições para modificação genética visada através da utilização de arn guia emparelhados |
| JP6840077B2 (ja) | 2014-12-19 | 2021-03-10 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | 単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物 |
| WO2016110512A1 (en) * | 2015-01-06 | 2016-07-14 | Dsm Ip Assets B.V. | A crispr-cas system for a yeast host cell |
| GB2531454A (en) | 2016-01-10 | 2016-04-20 | Snipr Technologies Ltd | Recombinogenic nucleic acid strands in situ |
| DK3320091T3 (da) * | 2015-07-06 | 2021-02-01 | Dsm Ip Assets Bv | Guide-rna-samlingsvektor |
| EP3328399B1 (en) * | 2015-07-31 | 2023-12-27 | Regents of the University of Minnesota | Modified cells and methods of therapy |
| WO2017048316A1 (en) * | 2015-09-18 | 2017-03-23 | President And Fellows Of Harvard College | Small molecule biosensors |
| US11667911B2 (en) | 2015-09-24 | 2023-06-06 | Editas Medicine, Inc. | Use of exonucleases to improve CRISPR/CAS-mediated genome editing |
| WO2017070632A2 (en) | 2015-10-23 | 2017-04-27 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| CN105469123A (zh) * | 2015-12-30 | 2016-04-06 | 华东理工大学 | 一种基于k平面回归的缺失数据补全方法 |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP4047092A1 (en) | 2016-04-13 | 2022-08-24 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| JP7093310B2 (ja) | 2016-05-18 | 2022-06-29 | アミリス， インコーポレイテッド | 外因性ランディングパッドへの核酸のゲノム組込みのための組成物および方法 |
| US11149268B2 (en) * | 2016-07-28 | 2021-10-19 | Dsm Ip Assets B.V. | Assembly system for a eukaryotic cell |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| GB2573062A (en) | 2016-10-14 | 2019-10-23 | Harvard College | AAV delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| WO2018131034A1 (en) * | 2017-01-11 | 2018-07-19 | Yeda Research And Development Co. Ltd. | Targeted recombination between homologous chromosomes and uses thereof |
| TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
| WO2018148410A1 (en) * | 2017-02-09 | 2018-08-16 | The Charles Stark Draper Laboratory Inc. | Bacteriophage recombination followed by blockage of nonrecombinant bacteriophage replication |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20190130613A (ko) | 2017-03-23 | 2019-11-22 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US20200377881A1 (en) * | 2017-03-24 | 2020-12-03 | President And Fellows Of Harvard College | Methods of Genome Engineering by Nuclease-Transposase Fusion Proteins |
| WO2018127611A1 (en) * | 2017-04-06 | 2018-07-12 | Dsm Ip Assets B.V. | Self-guiding integration construct (sgic) |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| US20200216858A1 (en) * | 2017-07-21 | 2020-07-09 | I'rom Group Co., Ltd. | Polynucleotide for modifying target sequence and use thereof |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| CN107299114B (zh) * | 2017-08-23 | 2021-08-27 | 中国科学院分子植物科学卓越创新中心 | 一种高效的酵母菌染色体融合方法 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| EP3679143A1 (en) * | 2017-09-08 | 2020-07-15 | Life Technologies Corporation | Methods for improved homologous recombination and compositions thereof |
| WO2019055878A2 (en) * | 2017-09-15 | 2019-03-21 | The Board Of Trustees Of The Leland Stanford Junior University | MULTIPLEX PRODUCTION AND BAR CODIFICATION OF GENETICALLY MODIFIED CELLS |
| WO2019055535A1 (en) | 2017-09-18 | 2019-03-21 | Amyris, Inc. | METHODS OF GENOMIC INTEGRATION FOR HOST CELLS COMPRISING HANSENULA AND ARXULA |
| CN111433369A (zh) * | 2017-09-18 | 2020-07-17 | 阿迈瑞斯公司 | 用于毕赤酵母和其他宿主细胞基因组整合的方法 |
| EP3684940A1 (en) | 2017-09-18 | 2020-07-29 | Amyris, Inc. | Methods for genetic engineering kluyveromyces host cells |
| CN111386343A (zh) | 2017-09-18 | 2020-07-07 | 阿迈瑞斯公司 | 用于克鲁维酵母宿主细胞基因组整合的方法 |
| WO2019079347A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | USES OF BASIC EDITORS ADENOSINE |
| CN108388767B (zh) * | 2018-03-16 | 2022-10-04 | 南京邮电大学 | Gpcr药物靶标生物学功能预测方法及其应用 |
| KR102179034B1 (ko) * | 2018-06-29 | 2020-11-16 | 연세대학교 산학협력단 | 세포 내재 반복 서열을 대상으로 탈아미노효소를 이용한 세포 바코딩 기술 개발 |
| BR112021000313A2 (pt) | 2018-07-12 | 2021-04-06 | Amyris, Inc. | Métodos para controlar taxas de alimentação de fermentação |
| WO2020047138A1 (en) * | 2018-08-29 | 2020-03-05 | Amyris, Inc. | Cells and methods for selection based assay |
| CN112955540A (zh) * | 2018-08-30 | 2021-06-11 | 因思科瑞普特公司 | 在自动化模块和仪器中对经核酸酶经编辑的序列的改进的检测 |
| EP3862430A4 (en) | 2018-10-04 | 2022-06-15 | Kaneka Corporation | DNA CONSTRUCT FOR USE IN PLANT GENO EDITING |
| US10519475B1 (en) | 2018-11-21 | 2019-12-31 | Amyris, Inc. | Biosynthesis of compounds in yeast |
| CN109666596A (zh) * | 2018-12-17 | 2019-04-23 | 陕西师范大学 | 一种产β-胡萝卜素的重组菌及利用Crispr-Cas9技术构建的方法 |
| CN113260700B (zh) * | 2018-12-30 | 2024-01-30 | 豪夫迈·罗氏有限公司 | 基于可检测标签与靶蛋白的CRISPR/Cas控制的整合而选择细胞的方法 |
| KR20210143230A (ko) | 2019-03-19 | 2021-11-26 | 더 브로드 인스티튜트, 인코퍼레이티드 | 뉴클레오티드 서열을 편집하기 위한 방법 및 조성물 |
| MX2021012157A (es) * | 2019-04-05 | 2022-01-06 | Danisco Us Inc | Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos. |
| WO2020257545A1 (en) * | 2019-06-19 | 2020-12-24 | Applied Stemcell, Inc. | Methods for chomosome rearrangement |
| JP2021000041A (ja) * | 2019-06-24 | 2021-01-07 | トヨタ自動車株式会社 | 形質転換補助用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法 |
| EP4004198A4 (en) * | 2019-07-23 | 2023-07-19 | Pioneer Hi-Bred International, Inc. | DONOR DESIGN STRATEGY FOR CRISPR-CAS9 GENOME EDITING |
| CN116096873A (zh) | 2020-05-08 | 2023-05-09 | 布罗德研究所股份有限公司 | 同时编辑靶标双链核苷酸序列的两条链的方法和组合物 |
| JP2022088751A (ja) * | 2020-12-03 | 2022-06-15 | トヨタ自動車株式会社 | 形質転換体の製造方法 |
| CN112522205B (zh) * | 2020-12-15 | 2023-06-02 | 广东省农业科学院动物卫生研究所 | 一种过表达血管紧张素转换酶2的细胞系及其制备方法与应用 |
| JP2022099419A (ja) * | 2020-12-23 | 2022-07-05 | トヨタ自動車株式会社 | 形質転換用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法 |
| JP2022099381A (ja) * | 2020-12-23 | 2022-07-05 | トヨタ自動車株式会社 | 形質転換補助用プラスミド及びこれを用いた形質転換体の製造方法、並びに形質転換方法 |
| JP2024509194A (ja) * | 2021-03-05 | 2024-02-29 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | インビボｄｎａアセンブリー及び解析 |
| CN113717874B (zh) * | 2021-09-27 | 2023-04-11 | 四川大学 | 一种耐高温、耐高糖的酿酒酵母菌株及其构建方法和应用 |
| WO2023225358A1 (en) * | 2022-05-20 | 2023-11-23 | The Board Of Trustees Of The Leland Stanford Junior University | Generation and tracking of cells with precise edits |
| WO2023230411A1 (en) | 2022-05-26 | 2023-11-30 | Amyris, Inc. | Improved oligosaccharide production in yeast |
| WO2023244843A1 (en) | 2022-06-16 | 2023-12-21 | Amyris, Inc. | HIGH pH METHODS AND COMPOSITIONS FOR CULTURING GENETICALLY MODIFIED HOST CELLS |
| WO2024042405A1 (en) | 2022-08-26 | 2024-02-29 | Amyris Bio Products Portugal, Unipessoal, Ltda. | Compositions and methods for the synthesis of bio-based polymers |
| WO2024042486A1 (en) | 2022-08-26 | 2024-02-29 | Amyris Bio Products Portugal, Unipessoal, Ltda. | Compositions and methods for the production of polyurethanes |
Family Cites Families (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5272065A (en) | 1983-10-20 | 1993-12-21 | Research Foundation Of State University Of New York | Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA |
| US4873192A (en) | 1987-02-17 | 1989-10-10 | The United States Of America As Represented By The Department Of Health And Human Services | Process for site specific mutagenesis without phenotypic selection |
| US6140466A (en) | 1994-01-18 | 2000-10-31 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
| NZ312332A (en) | 1995-06-07 | 2000-01-28 | Life Technologies Inc | Recombinational cloning using engineered recombination sites |
| US5976846A (en) * | 1996-01-13 | 1999-11-02 | Passmore; Steven E. | Method for multifragment in vivo cloning and mutation mapping |
| US5840579A (en) * | 1996-05-01 | 1998-11-24 | The Johns Hopkins University | Nucleic acids encoding p53 mutations which suppress p53 cancer mutations |
| JP4206154B2 (ja) | 1997-11-13 | 2009-01-07 | 大日本住友製薬株式会社 | 変異型ｌｏｘＰ配列とその応用 |
| NZ504511A (en) | 1997-11-18 | 2002-12-20 | Pioneer Hi Bred Int | Recombinant proteins comprising fused first and second distinct site specific recombinase and use in methods for the integration of foreign DNA into eukaryotic genomes |
| US5929301A (en) | 1997-11-18 | 1999-07-27 | Pioneer Hi-Bred International | Nucleic acid sequence encoding FLP recombinase |
| PT1034262E (pt) | 1997-11-18 | 2005-10-31 | Pioneer Hi Bred Int | Composicoes e metodos para a modificacao genetica de plantas |
| US6453242B1 (en) | 1999-01-12 | 2002-09-17 | Sangamo Biosciences, Inc. | Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites |
| US6794136B1 (en) | 2000-11-20 | 2004-09-21 | Sangamo Biosciences, Inc. | Iterative optimization in the design of binding proteins |
| DK1234022T3 (da) | 1999-07-23 | 2010-07-19 | Univ California | DNA-rekombination i eukaryote celler med bakteriofag PHIC31-rekombinationssystemet |
| WO2001011058A1 (en) | 1999-08-09 | 2001-02-15 | Monsanto Technology Llc | Novel cloning methods and vectors |
| WO2001023545A1 (fr) | 1999-09-30 | 2001-04-05 | Sumitomo Pharmaceuticals Company, Limited | Adn contenant des sequences frt allelomorphes |
| WO2001083819A2 (en) | 2000-04-28 | 2001-11-08 | Sangamo Biosciences, Inc. | Methods for designing exogenous regulatory molecules |
| US20030082561A1 (en) | 2000-07-21 | 2003-05-01 | Takashi Sera | Zinc finger domain recognition code and uses thereof |
| WO2002057294A2 (en) | 2001-01-22 | 2002-07-25 | Sangamo Biosciences, Inc. | Zinc finger proteins for dna binding and gene regulation in plants |
| GB0108491D0 (en) | 2001-04-04 | 2001-05-23 | Gendaq Ltd | Engineering zinc fingers |
| US20020165175A1 (en) * | 2001-04-17 | 2002-11-07 | Xiaowu Liang | Fast and enzymeless cloning of nucleic acid fragments |
| AT5356U1 (de) | 2001-07-16 | 2002-06-25 | Uv & P Umweltman Verfahrenstec | Verfahren zur immobilisierung von verbrennungsrückständen |
| JP2005520519A (ja) | 2002-03-15 | 2005-07-14 | セレクティス | ハイブリッドおよび単鎖メガヌクレアーゼならびにその使用 |
| WO2009095742A1 (en) | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
| WO2003080809A2 (en) | 2002-03-21 | 2003-10-02 | Sangamo Biosciences, Inc. | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
| AU2003290518A1 (en) | 2002-09-06 | 2004-04-23 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
| EP2559759A1 (en) | 2003-01-28 | 2013-02-20 | Cellectis | Custom-made meganuclease and use thereof |
| US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| EP2927318B1 (en) | 2003-08-08 | 2020-05-20 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
| EP1591521A1 (en) | 2004-04-30 | 2005-11-02 | Cellectis | I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof |
| WO2006097784A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | I-crei meganuclease variants with modified specificity, method of preparation and uses thereof |
| WO2007034262A1 (en) | 2005-09-19 | 2007-03-29 | Cellectis | Heterodimeric meganucleases and use thereof |
| ES2347684T3 (es) | 2005-03-15 | 2010-11-03 | Cellectis | Variantes de la meganucleasa i-crei con especificidad modifica, metodo de preparacion y usos de las mismas. |
| WO2007060495A1 (en) | 2005-10-25 | 2007-05-31 | Cellectis | I-crei homing endonuclease variants having novel cleavage specificity and use thereof |
| WO2007049095A1 (en) | 2005-10-25 | 2007-05-03 | Cellectis | Laglidadg homing endonuclease variants having mutations in two functional subdomains and use thereof |
| AU2007284748B2 (en) | 2006-08-11 | 2013-05-16 | Corteva Agriscience Llc | Zinc finger nuclease-mediated homologous recombination |
| WO2009001159A1 (en) | 2007-06-25 | 2008-12-31 | Cellectis | Method for enhancing the cleavage activity of i-crei derived meganucleases |
| US20140112904A9 (en) | 2007-06-06 | 2014-04-24 | Cellectis | Method for enhancing the cleavage activity of i-crei derived meganucleases |
| WO2010015899A2 (en) | 2008-08-04 | 2010-02-11 | Cellectis | Novel method to generate meganucleases with altered characteristics |
| US20110014616A1 (en) * | 2009-06-30 | 2011-01-20 | Sangamo Biosciences, Inc. | Rapid screening of biologically active nucleases and isolation of nuclease-modified cells |
| WO2009042186A2 (en) | 2007-09-27 | 2009-04-02 | Sangamo Biosciences, Inc. | Genomic editing in zebrafish using zinc finger nucleases |
| JP2011500082A (ja) | 2007-10-25 | 2011-01-06 | サンガモ バイオサイエンシーズ， インコーポレイテッド | 標的組込みのための方法および組成物 |
| WO2010001189A1 (en) | 2008-07-03 | 2010-01-07 | Cellectis | The crystal structure of i-dmoi in complex with its dna target, improved chimeric meganucleases and uses thereof |
| EP2180058A1 (en) | 2008-10-23 | 2010-04-28 | Cellectis | Meganuclease recombination system |
| CN102282255B (zh) | 2008-11-19 | 2014-05-07 | 阿迈瑞斯公司 | 多核苷酸装配的组合物和方法 |
| CA2745031C (en) | 2008-12-04 | 2018-08-14 | Sangamo Biosciences, Inc. | Genome editing in rats using zinc-finger nucleases |
| EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
| CN105274004B (zh) * | 2009-03-06 | 2019-08-09 | 合成基因组股份有限公司 | 用于克隆和操作基因组的方法 |
| TR201815882T4 (tr) | 2009-12-10 | 2018-11-21 | Univ Iowa State Res Found Inc | Tal efektörü aracılı dna modifikasyonu. |
| GB201009732D0 (en) * | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| US7919605B1 (en) * | 2010-08-30 | 2011-04-05 | Amyris, Inc. | Nucleic acids, compositions and methods for the excision of target nucleic acids |
| WO2012149470A1 (en) | 2011-04-27 | 2012-11-01 | Amyris, Inc. | Methods for genomic modification |
| JP6073350B2 (ja) | 2011-11-09 | 2017-02-01 | アミリス， インコーポレイテッド | アセチル−補酵素ａ誘導イソプレノイドの生産 |
| US8332160B1 (en) | 2011-11-17 | 2012-12-11 | Amyris Biotechnologies, Inc. | Systems and methods for engineering nucleic acid constructs using scoring techniques |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| JP6343605B2 (ja) | 2012-05-25 | 2018-06-13 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Ｒｎａ依存性標的ｄｎａ修飾およびｒｎａ依存性転写調節のための方法および組成物 |
| CN103382468B (zh) * | 2013-07-04 | 2015-04-29 | 中国科学院遗传与发育生物学研究所 | 一种水稻基因组定点改造方法 |
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| CN106029886A (zh) | 2016-10-12 |
| JP6652489B2 (ja) | 2020-02-26 |
| US10041092B2 (en) | 2018-08-07 |
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| US20150184199A1 (en) | 2015-07-02 |
| EP3083958A1 (en) | 2016-10-26 |
| US9476065B2 (en) | 2016-10-25 |
| MX2016007797A (es) | 2016-09-07 |
| AU2014368982B2 (en) | 2021-03-25 |
| EP3083958B1 (en) | 2019-04-17 |
| WO2015095804A1 (en) | 2015-06-25 |
| JP2017500038A (ja) | 2017-01-05 |
| US20220325303A1 (en) | 2022-10-13 |
| US20170058299A1 (en) | 2017-03-02 |
| CN106029886B (zh) | 2021-02-05 |
| US20190017074A1 (en) | 2019-01-17 |
| KR20160098481A (ko) | 2016-08-18 |
| AU2014368982A1 (en) | 2016-06-16 |
| US20200354746A1 (en) | 2020-11-12 |
| CA2933902C (en) | 2022-08-30 |
| US10626418B2 (en) | 2020-04-21 |
| CA2933902A1 (en) | 2015-06-25 |
| US11390888B2 (en) | 2022-07-19 |
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