DK1234022T3 - DNA-rekombination i eukaryote celler med bakteriofag PHIC31-rekombinationssystemet - Google Patents

DNA-rekombination i eukaryote celler med bakteriofag PHIC31-rekombinationssystemet

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Publication number
DK1234022T3
DK1234022T3 DK00950558.7T DK00950558T DK1234022T3 DK 1234022 T3 DK1234022 T3 DK 1234022T3 DK 00950558 T DK00950558 T DK 00950558T DK 1234022 T3 DK1234022 T3 DK 1234022T3
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DK
Denmark
Prior art keywords
recombination
eukaryotic cells
dna
bacteriophage phic31
bacteriophage
Prior art date
Application number
DK00950558.7T
Other languages
English (en)
Inventor
David W Ow
Richard Calendar
Lynn C Thomason
Original Assignee
Univ California
Us Of America Represented By T
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Us Of America Represented By T filed Critical Univ California
Application granted granted Critical
Publication of DK1234022T3 publication Critical patent/DK1234022T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/70—Vector systems having a special element relevant for transcription from fungi
    • C12N2830/702—Vector systems having a special element relevant for transcription from fungi yeast
    • C12N2830/706—S. pombe

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK00950558.7T 1999-07-23 2000-07-21 DNA-rekombination i eukaryote celler med bakteriofag PHIC31-rekombinationssystemet DK1234022T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14546999P 1999-07-23 1999-07-23
PCT/US2000/019983 WO2001007572A2 (en) 1999-07-23 2000-07-21 Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system

Publications (1)

Publication Number Publication Date
DK1234022T3 true DK1234022T3 (da) 2010-07-19

Family

ID=22513262

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00950558.7T DK1234022T3 (da) 1999-07-23 2000-07-21 DNA-rekombination i eukaryote celler med bakteriofag PHIC31-rekombinationssystemet

Country Status (8)

Country Link
EP (1) EP1234022B1 (da)
JP (2) JP4808880B2 (da)
AT (1) ATE463569T1 (da)
AU (1) AU6364800A (da)
CA (1) CA2379115C (da)
DE (1) DE60044142D1 (da)
DK (1) DK1234022T3 (da)
WO (1) WO2001007572A2 (da)

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US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
DE60042969D1 (de) 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US6746870B1 (en) 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
GB9921937D0 (en) * 1999-09-17 1999-11-17 Univ Leeds Targeted gens removal
AU2001277076A1 (en) 2000-07-21 2002-02-05 The United States Of America As Represented By The Secretary Of Agriculture Methods for the replacement, translocation and stacking of DNA in eukaryotic genomes
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
NZ545697A (en) * 2001-05-30 2008-06-30 Glaxo Group Ltd A lambda-intR mutein comprising a glutamic acid to arginine change at position 174 of Wt lambda-intR
AU2002366865A1 (en) * 2001-12-20 2003-07-09 Sungene Gmbh And Co. Kgaa Method for the transformation of vegetable plastids
CA2480642C (en) 2002-03-29 2012-10-16 Janet Louise Suttie Lambda integrase mediated recombination in plants
PT1583830E (pt) 2003-01-07 2006-11-30 Symphogen As Método para produzir proteínas policlonais recombinantes
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
EP1771483A1 (en) 2004-07-20 2007-04-11 Symphogen A/S Anti-rhesus d recombinant polyclonal antibody and methods of manufacture
WO2006007853A2 (en) 2004-07-20 2006-01-26 Symphogen A/S A procedure for structural characterization of a recombinant polyclonal protein or a polyclonal cell line
EP1907553B1 (en) 2005-07-18 2012-08-22 Pioneer Hi-Bred International Inc. Modified frt recombination sites and methods of use
MX2008014615A (es) 2006-05-17 2009-01-15 Pioneer Hi Bred Int Minicromosomas artificiales de plantas.
BRPI0816750A2 (pt) 2007-06-29 2015-09-29 Pioneer Hi Bred Int métodos para alterar o genoma de uma célula de planta monocotiledônea e para modificar uma sequência alvo genômica endógena específica e planta de milho
CN102939383B (zh) 2009-12-30 2015-04-29 先锋国际良种公司 用于靶向多核苷酸修饰的方法和组合物
JP6092782B2 (ja) 2010-11-16 2017-03-08 エクセリミューン， インコーポレイテッド 組換えタンパク質の製造方法
CA2831144A1 (en) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Methods for producing a complex transgenic trait locus
JP6158170B2 (ja) 2011-04-27 2017-07-12 アミリス， インコーポレイテッド ゲノム修飾のための方法
BR112013033176A2 (pt) 2011-06-21 2018-06-12 E I Du Pont De Nemouras And Company método para produzir uma modificação direcionada em um gene de fertilidade masculina, planta, molécula de ácido nucleio isolada, construto de expressão
EP2739738B1 (en) 2011-08-03 2018-06-20 Ramot at Tel Aviv University, Ltd. Use of integrase for targeted gene expression
US20140173781A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic wheat plants
US10597662B2 (en) 2013-11-22 2020-03-24 Jmtc Enzyme Corporation Transformant and process for production thereof, and process for production of lactic acid
JP6515502B2 (ja) * 2013-11-22 2019-05-22 Ａｇｃ株式会社 形質転換体の製造方法、形質転換体、および単座組込み用ベクターキット
PT3083958T (pt) 2013-12-19 2019-06-27 Amyris Inc Métodos de integração genómica
ES2759269T3 (es) 2014-10-10 2020-05-08 Jmtc Enzyme Corp Transformante y procedimiento para la producción del mismo, y procedimiento para la producción de ácido láctico
EP4219731A3 (en) 2016-05-18 2023-08-09 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
CN108410766B (zh) * 2018-03-16 2021-03-05 中国科学院化学研究所 共轭聚合物pmnt在促进肠道有益菌粘附及其生物膜形成的应用

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SE9703663D0 (sv) * 1997-10-08 1997-10-08 Pharmacia & Upjohn Ab Beta recombinase
US6632672B2 (en) * 1998-08-19 2003-10-14 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification

Also Published As

Publication number Publication date
JP4808880B2 (ja) 2011-11-02
EP1234022A2 (en) 2002-08-28
CA2379115A1 (en) 2001-02-01
DE60044142D1 (de) 2010-05-20
EP1234022B1 (en) 2010-04-07
WO2001007572A2 (en) 2001-02-01
CA2379115C (en) 2011-04-26
ATE463569T1 (de) 2010-04-15
WO2001007572A3 (en) 2001-04-19
JP2003505065A (ja) 2003-02-12
AU6364800A (en) 2001-02-13
JP2011045381A (ja) 2011-03-10

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