ATE463569T1 - Dna-rekombination in eukaryotischen zellen mittels des rekombinationszentrums vom bakteriophag phic31 - Google Patents

Dna-rekombination in eukaryotischen zellen mittels des rekombinationszentrums vom bakteriophag phic31

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Publication number
ATE463569T1
ATE463569T1 AT00950558T AT00950558T ATE463569T1 AT E463569 T1 ATE463569 T1 AT E463569T1 AT 00950558 T AT00950558 T AT 00950558T AT 00950558 T AT00950558 T AT 00950558T AT E463569 T1 ATE463569 T1 AT E463569T1
Authority
AT
Austria
Prior art keywords
recombination
eukaryotic cells
center
dna
bacteriophage phic31
Prior art date
Application number
AT00950558T
Other languages
English (en)
Inventor
David Ow
Richard Calendar
Lynn C Thomason
Original Assignee
Univ California
Us Of America Represented By T
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Us Of America Represented By T filed Critical Univ California
Application granted granted Critical
Publication of ATE463569T1 publication Critical patent/ATE463569T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/70—Vector systems having a special element relevant for transcription from fungi
    • C12N2830/702—Vector systems having a special element relevant for transcription from fungi yeast
    • C12N2830/706—S. pombe

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT00950558T 1999-07-23 2000-07-21 Dna-rekombination in eukaryotischen zellen mittels des rekombinationszentrums vom bakteriophag phic31 ATE463569T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14546999P 1999-07-23 1999-07-23
PCT/US2000/019983 WO2001007572A2 (en) 1999-07-23 2000-07-21 Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system

Publications (1)

Publication Number Publication Date
ATE463569T1 true ATE463569T1 (de) 2010-04-15

Family

ID=22513262

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00950558T ATE463569T1 (de) 1999-07-23 2000-07-21 Dna-rekombination in eukaryotischen zellen mittels des rekombinationszentrums vom bakteriophag phic31

Country Status (8)

Country Link
EP (1) EP1234022B1 (de)
JP (2) JP4808880B2 (de)
AT (1) ATE463569T1 (de)
AU (1) AU6364800A (de)
CA (1) CA2379115C (de)
DE (1) DE60044142D1 (de)
DK (1) DK1234022T3 (de)
WO (1) WO2001007572A2 (de)

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US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
AU752704B2 (en) 1997-10-24 2002-09-26 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
EP2336148A1 (de) 1999-03-02 2011-06-22 Life Technologies Corporation Zusammensetzungen und Verfahren zur Verwendung beim Rekombinationsklonen von Nukleinsäuren
US6746870B1 (en) 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
GB9921937D0 (en) * 1999-09-17 1999-11-17 Univ Leeds Targeted gens removal
EP1978098A3 (de) 1999-12-10 2009-02-18 Invitrogen Corporation Verwendung von mehrfachen Rekombinationsstellen mit einzigartiger Spezifizität beim Rekombinationsklonen
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002008409A2 (en) 2000-07-21 2002-01-31 The United States Of America, As Represented By The Secretary Of Agriculture Methods for the replacement, translocation and stacking of dna in eukaryotic genomes
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
MXPA03010626A (es) * 2001-05-30 2004-12-06 Chromos Molecular Systems Inc Plataformas a base de cromosomas.
AU2002366865A1 (en) * 2001-12-20 2003-07-09 Sungene Gmbh And Co. Kgaa Method for the transformation of vegetable plastids
US7351877B2 (en) 2002-03-29 2008-04-01 Syngenta Participations Ag Lambda integrase mediated recombination in plants
EA013225B1 (ru) 2003-01-07 2010-04-30 Симфоген А/С Способ получения линии клеток, продуцирующей рекомбинантный поликлональный белок, способ получения поликлонального белка, линия клеток, продуцирующая рекомбинантный поликлональный белок, библиотека векторов, популяция клеток
EP2287341B1 (de) 2003-12-01 2013-02-13 Life Technologies Corporation Rekombinationsstellen enthaltende Nukleinsäuremoleküle und Verfahren zur Verwendung davon
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
DK2053408T3 (da) 2004-07-20 2012-06-04 Symphogen As Fremgangsmåde til strukturel karakterisering af et rekombinant polyklonalt protein eller en polyklonal cellelinje
MX2007000644A (es) 2004-07-20 2007-03-28 Symphogen As Anticuerpo policlonal recombinante anti-rhesus d y metodos de fabricacion.
EP1907553B1 (de) 2005-07-18 2012-08-22 Pioneer Hi-Bred International Inc. Modifizierte frt-rekombinationsstellen und verwendungsverfahren
EP2308986B1 (de) 2006-05-17 2014-10-08 Pioneer Hi-Bred International Inc. Künstliche Pflanzenminichromosome
CN101849010A (zh) 2007-06-29 2010-09-29 先锋高级育种国际公司 用于改变单子叶植物细胞的基因组的方法
EP2529017A2 (de) 2009-12-30 2012-12-05 Pioneer Hi-Bred International, Inc. Verfahren und zusammensetzungen für gezielte polynukleotidmodifikation
WO2012068317A2 (en) 2010-11-16 2012-05-24 Excelimmune, Inc. Methods for producing recombinant proteins
MX2013010911A (es) 2011-03-23 2015-03-03 Pioneer Hi Bred Int Metodos para producir un locus de rasgo transgenico complejo.
CA2834375C (en) 2011-04-27 2020-07-14 Amyris, Inc. Methods for genomic modification
CN104080914A (zh) 2011-06-21 2014-10-01 先锋国际良种公司 产生雄性不育植物的组合物和方法
WO2013018096A1 (en) 2011-08-03 2013-02-07 Ramot At Tel-Aviv University Ltd. Use of integrase for targeted gene expression
US20140173781A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic wheat plants
JP6515502B2 (ja) * 2013-11-22 2019-05-22 Ａｇｃ株式会社 形質転換体の製造方法、形質転換体、および単座組込み用ベクターキット
EP3072955B1 (de) 2013-11-22 2019-01-09 JMTC Enzyme Corporation Transformant und verfahren zur herstellung davon sowie verfahren zur herstellung von milchsäure
MX2016007797A (es) 2013-12-19 2016-09-07 Amyris Inc Metodos para integracion genomica.
EP3205721B1 (de) 2014-10-10 2019-08-28 JMTC Enzyme Corporation Transformant und verfahren zur herstellung davon und verfahren zur herstellung von milchsäure
JP7093310B2 (ja) 2016-05-18 2022-06-29 アミリス， インコーポレイテッド 外因性ランディングパッドへの核酸のゲノム組込みのための組成物および方法
CN108410766B (zh) * 2018-03-16 2021-03-05 中国科学院化学研究所 共轭聚合物pmnt在促进肠道有益菌粘附及其生物膜形成的应用

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SE9703663D0 (sv) * 1997-10-08 1997-10-08 Pharmacia & Upjohn Ab Beta recombinase
WO2000011155A1 (en) * 1998-08-19 2000-03-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification

Also Published As

Publication number Publication date
CA2379115A1 (en) 2001-02-01
EP1234022B1 (de) 2010-04-07
JP2003505065A (ja) 2003-02-12
EP1234022A2 (de) 2002-08-28
JP4808880B2 (ja) 2011-11-02
AU6364800A (en) 2001-02-13
JP2011045381A (ja) 2011-03-10
DK1234022T3 (da) 2010-07-19
CA2379115C (en) 2011-04-26
WO2001007572A3 (en) 2001-04-19
WO2001007572A2 (en) 2001-02-01
DE60044142D1 (de) 2010-05-20

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