WO2023220649A3 - Effector protein compositions and methods of use thereof - Google Patents

Effector protein compositions and methods of use thereof Download PDF

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Publication number
WO2023220649A3
WO2023220649A3 PCT/US2023/066843 US2023066843W WO2023220649A3 WO 2023220649 A3 WO2023220649 A3 WO 2023220649A3 US 2023066843 W US2023066843 W US 2023066843W WO 2023220649 A3 WO2023220649 A3 WO 2023220649A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
effector protein
protein compositions
compositions
effector proteins
Prior art date
Application number
PCT/US2023/066843
Other languages
French (fr)
Other versions
WO2023220649A2 (en
Inventor
Sean Chen
Lucas Benjamin HARRINGTON
William Douglass WRIGHT
Aaron DELOUGHERY
Wiputra Jaya HARTONO
Benjamin Julius RAUCH
Stepan TYMOSHENKO
Original Assignee
Mammoth Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mammoth Biosciences, Inc. filed Critical Mammoth Biosciences, Inc.
Publication of WO2023220649A2 publication Critical patent/WO2023220649A2/en
Publication of WO2023220649A3 publication Critical patent/WO2023220649A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Provided herein are compositions, systems, and methods comprising effector proteins and uses thereof. These effector proteins may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure may leverage the activities of these effector proteins for the modification and engineering of nucleic acids.
PCT/US2023/066843 2022-05-10 2023-05-10 Effector protein compositions and methods of use thereof WO2023220649A2 (en)

Applications Claiming Priority (18)

Application Number Priority Date Filing Date Title
US202263340371P 2022-05-10 2022-05-10
US202263340374P 2022-05-10 2022-05-10
US63/340,371 2022-05-10
US63/340,374 2022-05-10
US202263353963P 2022-06-21 2022-06-21
US63/353,963 2022-06-21
US202263390576P 2022-07-19 2022-07-19
US202263390499P 2022-07-19 2022-07-19
US63/390,576 2022-07-19
US63/390,499 2022-07-19
US202263383836P 2022-11-15 2022-11-15
US202263383860P 2022-11-15 2022-11-15
US63/383,860 2022-11-15
US63/383,836 2022-11-15
US202363483934P 2023-02-08 2023-02-08
US202363483951P 2023-02-08 2023-02-08
US63/483,934 2023-02-08
US63/483,951 2023-02-08

Publications (2)

Publication Number Publication Date
WO2023220649A2 WO2023220649A2 (en) 2023-11-16
WO2023220649A3 true WO2023220649A3 (en) 2023-12-28

Family

ID=88731149

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/066843 WO2023220649A2 (en) 2022-05-10 2023-05-10 Effector protein compositions and methods of use thereof

Country Status (1)

Country Link
WO (1) WO2023220649A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080176285A1 (en) * 1999-09-14 2008-07-24 Genzyme Glycobiology Research Institute, Inc. Glcnac phosphotransferase of the lysosomal targeting pathway
US20200318136A1 (en) * 2019-04-03 2020-10-08 Regeneron Pharmaceuticals, Inc. Methods and compositions for insertion of antibody coding sequences into a safe harbor locus
WO2021072115A1 (en) * 2019-10-08 2021-04-15 Regents Of The University Of Minnesota Crispr-mediated human genome editing with vectors
WO2021247924A1 (en) * 2020-06-03 2021-12-09 Mammoth Biosciences, Inc. Programmable nucleases and methods of use

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080176285A1 (en) * 1999-09-14 2008-07-24 Genzyme Glycobiology Research Institute, Inc. Glcnac phosphotransferase of the lysosomal targeting pathway
US20200318136A1 (en) * 2019-04-03 2020-10-08 Regeneron Pharmaceuticals, Inc. Methods and compositions for insertion of antibody coding sequences into a safe harbor locus
WO2021072115A1 (en) * 2019-10-08 2021-04-15 Regents Of The University Of Minnesota Crispr-mediated human genome editing with vectors
WO2021247924A1 (en) * 2020-06-03 2021-12-09 Mammoth Biosciences, Inc. Programmable nucleases and methods of use

Also Published As

Publication number Publication date
WO2023220649A2 (en) 2023-11-16

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