MX2013010911A - Metodos para producir un locus de rasgo transgenico complejo. - Google Patents

Metodos para producir un locus de rasgo transgenico complejo.

Info

Publication number
MX2013010911A
MX2013010911A MX2013010911A MX2013010911A MX2013010911A MX 2013010911 A MX2013010911 A MX 2013010911A MX 2013010911 A MX2013010911 A MX 2013010911A MX 2013010911 A MX2013010911 A MX 2013010911A MX 2013010911 A MX2013010911 A MX 2013010911A
Authority
MX
Mexico
Prior art keywords
transgenic trait
complex transgenic
methods
producing
trait locus
Prior art date
Application number
MX2013010911A
Other languages
English (en)
Inventor
Michael Lassner
Derek Jantz
James Jefferson Smith
Mark Cigan
Carl Falco
Huirong Gao
Zhongsen Li
Zhan-Bin Liu
Sergei Svitashev
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of MX2013010911A publication Critical patent/MX2013010911A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se describen métodos para producir en una planta un locus de rasgo transgénico complejo que comprende por lo menos dos secuencias objetivo alteradas en una región genómica de interés. Los métodos involucran el uso de dos o más agentes de inducción de ruptura de doble hebra, cada uno de los cuales puede causar una ruptura de doble hebra en una secuencia objetivo en la región genómica de interés que da por resultado una alteración en la secuencia objetivo. También se describen loci de rasgo transgénico complejo en plantas. Un locus de rasgo transgénico complejo comprende por lo menos dos secuencias objetivo alteradas que se enlazan genéticamente a un polinucleótido de interés. También se describen plantas, células de planta, partes de planta y semillas que comprenden uno o más loci de rasgo transgénico complejo.
MX2013010911A 2011-03-23 2012-03-22 Metodos para producir un locus de rasgo transgenico complejo. MX2013010911A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161466602P 2011-03-23 2011-03-23
US201161499443P 2011-06-21 2011-06-21
PCT/US2012/030061 WO2012129373A2 (en) 2011-03-23 2012-03-22 Methods for producing a complex transgenic trait locus

Publications (1)

Publication Number Publication Date
MX2013010911A true MX2013010911A (es) 2015-03-03

Family

ID=45929041

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013010911A MX2013010911A (es) 2011-03-23 2012-03-22 Metodos para producir un locus de rasgo transgenico complejo.

Country Status (10)

Country Link
US (4) US10030245B2 (es)
EP (1) EP2689019A2 (es)
CN (1) CN103842511A (es)
AR (1) AR085533A1 (es)
BR (1) BR112013024337A2 (es)
CA (1) CA2831144A1 (es)
EA (1) EA201391373A1 (es)
MX (1) MX2013010911A (es)
WO (1) WO2012129373A2 (es)
ZA (1) ZA201307200B (es)

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