MX2014009023A - Metodos y composiciones para generar loci de rasgo complejo. - Google Patents

Metodos y composiciones para generar loci de rasgo complejo.

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Publication number
MX2014009023A
MX2014009023A MX2014009023A MX2014009023A MX2014009023A MX 2014009023 A MX2014009023 A MX 2014009023A MX 2014009023 A MX2014009023 A MX 2014009023A MX 2014009023 A MX2014009023 A MX 2014009023A MX 2014009023 A MX2014009023 A MX 2014009023A
Authority
MX
Mexico
Prior art keywords
genomic
interest
complex trait
compositions
locus
Prior art date
Application number
MX2014009023A
Other languages
English (en)
Inventor
Michael Lassner
Zhongsen Li
Saveri Carl Falco
Christopher J Scelonge
Original Assignee
Pioner Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioner Hi Bred International Inc filed Critical Pioner Hi Bred International Inc
Publication of MX2014009023A publication Critical patent/MX2014009023A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)

Abstract

Se proporcionan composiciones y métodos para apilar múltiples loci transgénicos independientes en el genoma de una planta. Las composiciones incluyen plantas, semillas o células de plantas que comprenden por lo menos un sitio objetivo transgénico y por lo menos un locus genómico de interés integrado en diferentes sitios genómicos dentro de una ventana genómica. Se pueden emplear técnicas de reproducción de plantas tal que el sitio objetivo transgénico y el locus genómico de interés se pueden reproducir conjuntamente. De esta manera, múltiples integraciones de transgén independientes se pueden generar dentro de una ventana genómica para crear un locus de rasgo complejo. El locus de rasgo complejo se diseña tal que los sitios objetivo transgénicos y/o loci genómicos de interés pueden segregarse independientemente entre sí, para de esta manera proporcionar el beneficio de alterar un locus de rasgo complejo mediante la reproducción en, y la reproducción lejos de los elementos específicos. También se pueden emplear varios métodos para modificar los sitios objetivo tal que contienen una variedad de polinucleótidos de interés.
MX2014009023A 2012-01-27 2013-01-24 Metodos y composiciones para generar loci de rasgo complejo. MX2014009023A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261591329P 2012-01-27 2012-01-27
PCT/US2013/022891 WO2013112686A1 (en) 2012-01-27 2013-01-24 Methods and compositions for generating complex trait loci

Publications (1)

Publication Number Publication Date
MX2014009023A true MX2014009023A (es) 2015-03-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014009023A MX2014009023A (es) 2012-01-27 2013-01-24 Metodos y composiciones para generar loci de rasgo complejo.

Country Status (10)

Country Link
US (4) US10435699B2 (es)
EP (1) EP2807257A1 (es)
CN (1) CN104245939A (es)
AR (1) AR089793A1 (es)
BR (1) BR112014018560A8 (es)
CA (1) CA2862844A1 (es)
EA (1) EA201491431A1 (es)
MX (1) MX2014009023A (es)
WO (1) WO2013112686A1 (es)
ZA (1) ZA201405779B (es)

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Also Published As

Publication number Publication date
AR089793A1 (es) 2014-09-17
US20220251586A1 (en) 2022-08-11
ZA201405779B (en) 2015-07-29
EA201491431A1 (ru) 2015-01-30
CN104245939A (zh) 2014-12-24
WO2013112686A1 (en) 2013-08-01
EP2807257A1 (en) 2014-12-03
US11788097B2 (en) 2023-10-17
US11312969B2 (en) 2022-04-26
BR112014018560A2 (es) 2017-06-20
US20240093214A1 (en) 2024-03-21
CA2862844A1 (en) 2013-08-01
US10435699B2 (en) 2019-10-08
BR112014018560A8 (pt) 2017-07-11
US20130198888A1 (en) 2013-08-01
US20190376073A1 (en) 2019-12-12

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