DE69836641D1 - Gebrauch von beta-rekombinase in eukaryotischen zellen, speziell für transgene arbeiten - Google Patents

Gebrauch von beta-rekombinase in eukaryotischen zellen, speziell für transgene arbeiten

Info

Publication number
DE69836641D1
DE69836641D1 DE69836641T DE69836641T DE69836641D1 DE 69836641 D1 DE69836641 D1 DE 69836641D1 DE 69836641 T DE69836641 T DE 69836641T DE 69836641 T DE69836641 T DE 69836641T DE 69836641 D1 DE69836641 D1 DE 69836641D1
Authority
DE
Germany
Prior art keywords
eukaryotic cells
beta recombinase
transgenic
specially
works
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69836641T
Other languages
English (en)
Other versions
DE69836641T2 (de
Inventor
Vicente Diaz
Fernando Rojo
Alonso Carlos Martinez
Juan C Alonso
Antonio Bernard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Pharmacia Spain SA
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Pharmacia Spain SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, Pharmacia Spain SA filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Publication of DE69836641D1 publication Critical patent/DE69836641D1/de
Application granted granted Critical
Publication of DE69836641T2 publication Critical patent/DE69836641T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
DE69836641T 1997-10-08 1998-09-22 Verwendung einer prokaryotischen beta-Rekombinase in eukaryotischen Zellen für transgene Arbeit Expired - Fee Related DE69836641T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9703663A SE9703663D0 (sv) 1997-10-08 1997-10-08 Beta recombinase
SE9703663 1997-10-08
PCT/SE1998/001693 WO1999018222A1 (en) 1997-10-08 1998-09-22 Use of beta recombinase in eukaryotic cells, especially for transgenic work

Publications (2)

Publication Number Publication Date
DE69836641D1 true DE69836641D1 (de) 2007-01-25
DE69836641T2 DE69836641T2 (de) 2007-09-27

Family

ID=20408539

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69836641T Expired - Fee Related DE69836641T2 (de) 1997-10-08 1998-09-22 Verwendung einer prokaryotischen beta-Rekombinase in eukaryotischen Zellen für transgene Arbeit

Country Status (10)

Country Link
EP (1) EP1021550B1 (de)
JP (1) JP2001519163A (de)
AT (1) ATE348179T1 (de)
AU (1) AU734317B2 (de)
CA (1) CA2305815A1 (de)
DE (1) DE69836641T2 (de)
ES (1) ES2281933T3 (de)
NZ (1) NZ504189A (de)
SE (1) SE9703663D0 (de)
WO (1) WO1999018222A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780644B1 (en) * 1997-10-08 2004-08-24 Pharmacia Spain S.A. Use of beta recombinase in eukaryotic cells, especially for transgenic work
US6746870B1 (en) 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
WO2001007572A2 (en) * 1999-07-23 2001-02-01 The Regents Of The University Of California Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system
AU2001277076A1 (en) 2000-07-21 2002-02-05 The United States Of America As Represented By The Secretary Of Agriculture Methods for the replacement, translocation and stacking of DNA in eukaryotic genomes
EP1322152A2 (de) * 2000-10-03 2003-07-02 ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire Transgene maus zur gezielten rekombination vermittelt durch verändertes cre-er
FR2814642B1 (fr) * 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
GB0216203D0 (en) * 2002-07-12 2002-08-21 Imp Cancer Res Tech Mondulation of cytochrome P450 reductase activity
CN109957557B (zh) * 2017-12-26 2023-01-06 广州市锐博生物科技有限公司 Dna聚合酶及其制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU724922B2 (en) * 1995-06-07 2000-10-05 Invitrogen Corporation Recombinational cloning using engineered recombination sites

Also Published As

Publication number Publication date
WO1999018222A1 (en) 1999-04-15
EP1021550A1 (de) 2000-07-26
ATE348179T1 (de) 2007-01-15
AU9466898A (en) 1999-04-27
NZ504189A (en) 2003-03-28
CA2305815A1 (en) 1999-04-15
JP2001519163A (ja) 2001-10-23
AU734317B2 (en) 2001-06-07
DE69836641T2 (de) 2007-09-27
EP1021550B1 (de) 2006-12-13
ES2281933T3 (es) 2007-10-01
SE9703663D0 (sv) 1997-10-08

Similar Documents

Publication Publication Date Title
BR9813608B1 (pt) célula hospedeira de bacillus transgênica, e, processo para produzir um polipeptìdeo tendo atividade aminopeptidase.
ATE323770T1 (de) Polypeptide mit lysophospholipaseaktivität und nukleinesäuren die sie kodieren
ATE466948T1 (de) Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle
DE60044142D1 (de) Dna-rekombination in eukaryotischen zellen mittels des rekombinationszentrums vom bakteriophag phic31
PT981637E (pt) Metodo para integrar genes em sitios especificos em celulas de mamifero atraves de recombinacao homologa e vectores para a realizacao do mesmo
ATE317440T1 (de) Neuartige methode zur integration von fremd-dna ins eukaryotische genom
ES2181900T3 (es) Clonacion recombinante in vitro usanto sitios de recombinacion por ingenieria genetica.
DE69836641D1 (de) Gebrauch von beta-rekombinase in eukaryotischen zellen, speziell für transgene arbeiten
DE69840230D1 (de) Polypeptide mit prolyldipeptidylaminopeptidase-aktivität und dafür kodierende nukleinsäuren
AU2002304258A1 (en) Method for the stable inversion of dna sequence by site-specific recombination and dna vectors and transgenic cells thereof
HK1158529A1 (en) Promoters exhibiting endothelial cell specificity and methods of using same
ATE384787T1 (de) Tripeptidyl-aminopeptidase
BR9500271B1 (pt) seqüência de dna recombinante, vetor, células procarióticas e de fungo transformadas e proteìna com atividade sacarose-isomerase e com atividade de platinase e/ou trehalulase.
DE69837475D1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
DK1025245T3 (da) Hurtig etablering af stabile pattedyrscelelinjer, der producerer höje koncentrationer af rekombinante proteiner
AR017368A1 (es) Un procedimiento para la insercion de adn foraneo en una celula hospedante, un vector recombinante utilizado en dicho procedimiento y el uso del mismo
ATE414771T1 (de) Galaninrezeptor, nukleinsäuren,transformierte zellen und verwendungen
AR016263A1 (es) Un constructo de adn que altera la expresion de un gen g-csf endogeno en una celula de mamifero en la integracion al genoma de la celula a traves derecombinacion homologa, una celula recombinante homologamente establemente transfectada con el constructo de adn, un metodo de alteracion de la expresio
ATE288488T1 (de) Zusammensetzungen und verfahren zur eliminierung unerwünschter zellen
GB2321702B (en) Methods and means relating to retrotransposon and retroviral integration
ATE288491T1 (de) Polypeptide mit 5-aminolevulinsäure synthaseaktivität und deren nukleinsäuren
WO2003070931A3 (de) Verfahren zur ortsspezifischen dna-rekombination
BR9912407A (pt) alcool desidrodiconiferìlico éter benzìlicoredutase recombinante e métodos de uso
ZA200202863B (en) Novel cell cycle checkpoint genes and proteins encoded thereby.
Hearle Can genetic engineering enhance the miracle of wool? Pt. 2: the link from structure to properties

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee