MX2021012157A - Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos. - Google Patents
Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos.Info
- Publication number
- MX2021012157A MX2021012157A MX2021012157A MX2021012157A MX2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A
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- bacillus
- genome
- methods
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- recombinant dna
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
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- C12N15/09—Recombinant DNA-technology
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
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- C12Y304/21014—Microbial serine proteases (3.4.21.14)
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
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- Crystallography & Structural Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Se proporcionan métodos y composiciones para la integración de genes de interés en el genoma de una célula de Bacillus sp. sin la integración de un marcador seleccionable en los genoma. Los métodos emplean un sistema de ADN recombinante circular dual para la introducción de un sistema de endonucleasa ARN guía/Cas (también denominado endonucleasa guiada por ARN, RGEN), así como un ADN donante en una célula de Bacillus sp., y proporcionando un sistema altamente eficaz para insertar genes de interés en el genoma de la célula de Bacillus sp.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962829664P | 2019-04-05 | 2019-04-05 | |
PCT/US2020/026503 WO2020206197A1 (en) | 2019-04-05 | 2020-04-03 | Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2021012157A true MX2021012157A (es) | 2022-01-06 |
Family
ID=70476371
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2021012157A MX2021012157A (es) | 2019-04-05 | 2020-04-03 | Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos. |
Country Status (7)
Country | Link |
---|---|
US (1) | US20220162621A1 (es) |
EP (1) | EP3947656A1 (es) |
JP (1) | JP2022526414A (es) |
KR (1) | KR20210148270A (es) |
CA (1) | CA3136113A1 (es) |
MX (1) | MX2021012157A (es) |
WO (1) | WO2020206197A1 (es) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4359424A1 (en) | 2021-06-24 | 2024-05-01 | Basf Se | Improved bacillus production host |
BR112023027009A2 (pt) | 2021-06-24 | 2024-03-12 | Basf Se | Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse |
WO2022269084A1 (en) | 2021-06-24 | 2022-12-29 | Basf Se | Improved bacillus host cell with altered rema/remb protein |
WO2023117970A1 (en) | 2021-12-20 | 2023-06-29 | Basf Se | Method for improved production of intracellular proteins in bacillus |
CN116042401A (zh) * | 2023-02-20 | 2023-05-02 | 厦门大学 | 从环境样品中靶向分离纯化目标微生物的方法 |
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US5990387A (en) | 1988-06-10 | 1999-11-23 | Pioneer Hi-Bred International, Inc. | Stable transformation of plant cells |
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US5034323A (en) | 1989-03-30 | 1991-07-23 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
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TW261517B (es) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
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IL108241A (en) | 1992-12-30 | 2000-08-13 | Biosource Genetics Corp | Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus |
US5736369A (en) | 1994-07-29 | 1998-04-07 | Pioneer Hi-Bred International, Inc. | Method for producing transgenic cereal plants |
ATE229977T1 (de) | 1996-03-26 | 2003-01-15 | Razvan T Radulescu | Peptide mit antiproliferativen eigenschaften |
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EP2325332B1 (en) | 2005-08-26 | 2012-10-31 | DuPont Nutrition Biosciences ApS | Use of CRISPR associated genes (CAS) |
WO2008007989A1 (en) | 2006-07-11 | 2008-01-17 | Grabania, Bogdan | Head for directing objects, especially for displaying screens |
CN104053780A (zh) | 2011-12-09 | 2014-09-17 | 丹尼斯科美国公司 | 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子 |
DK3401400T3 (da) | 2012-05-25 | 2019-06-03 | Univ California | Fremgangsmåder og sammensætninger til rna-styret mål-dna-modifikation og til rna-styret transskriptionsmodulering |
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KR102274445B1 (ko) * | 2013-12-19 | 2021-07-08 | 아미리스 인코퍼레이티드 | 게놈 삽입을 위한 방법 |
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DK3368655T3 (da) | 2015-10-30 | 2020-09-14 | Danisco Us Inc | Forbedret proteinekspression og fremgangsmåder dertil |
EP4353828A2 (en) | 2017-02-24 | 2024-04-17 | Danisco US Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
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2020
- 2020-04-03 WO PCT/US2020/026503 patent/WO2020206197A1/en unknown
- 2020-04-03 KR KR1020217035668A patent/KR20210148270A/ko active Search and Examination
- 2020-04-03 EP EP20722758.8A patent/EP3947656A1/en active Pending
- 2020-04-03 US US17/601,550 patent/US20220162621A1/en active Pending
- 2020-04-03 JP JP2021559247A patent/JP2022526414A/ja active Pending
- 2020-04-03 CA CA3136113A patent/CA3136113A1/en active Pending
- 2020-04-03 MX MX2021012157A patent/MX2021012157A/es unknown
Also Published As
Publication number | Publication date |
---|---|
US20220162621A1 (en) | 2022-05-26 |
CA3136113A1 (en) | 2020-10-08 |
KR20210148270A (ko) | 2021-12-07 |
WO2020206197A1 (en) | 2020-10-08 |
JP2022526414A (ja) | 2022-05-24 |
EP3947656A1 (en) | 2022-02-09 |
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