MX2021012157A - Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos. - Google Patents

Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos.

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Publication number
MX2021012157A
MX2021012157A MX2021012157A MX2021012157A MX2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A
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Mexico
Prior art keywords
bacillus
genome
methods
compositions
recombinant dna
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MX2021012157A
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English (en)
Inventor
Wonchul Suh
Ryan L Frisch
Stacey Irene Robida Stubbs
Derek Joseph Zimmer
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Danisco Us Inc
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Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2021012157A publication Critical patent/MX2021012157A/es

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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
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Abstract

Se proporcionan métodos y composiciones para la integración de genes de interés en el genoma de una célula de Bacillus sp. sin la integración de un marcador seleccionable en los genoma. Los métodos emplean un sistema de ADN recombinante circular dual para la introducción de un sistema de endonucleasa ARN guía/Cas (también denominado endonucleasa guiada por ARN, RGEN), así como un ADN donante en una célula de Bacillus sp., y proporcionando un sistema altamente eficaz para insertar genes de interés en el genoma de la célula de Bacillus sp.
MX2021012157A 2019-04-05 2020-04-03 Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos. MX2021012157A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962829664P 2019-04-05 2019-04-05
PCT/US2020/026503 WO2020206197A1 (en) 2019-04-05 2020-04-03 Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof

Publications (1)

Publication Number Publication Date
MX2021012157A true MX2021012157A (es) 2022-01-06

Family

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MX2021012157A MX2021012157A (es) 2019-04-05 2020-04-03 Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos.

Country Status (7)

Country Link
US (1) US20220162621A1 (es)
EP (1) EP3947656A1 (es)
JP (1) JP2022526414A (es)
KR (1) KR20210148270A (es)
CA (1) CA3136113A1 (es)
MX (1) MX2021012157A (es)
WO (1) WO2020206197A1 (es)

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CN117813316A (zh) 2021-06-24 2024-04-02 巴斯夫欧洲公司 用于生产纯度更高的感兴趣的化合物的地衣芽孢杆菌宿主细胞
WO2022269082A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus production host
CN117693587A (zh) 2021-06-24 2024-03-12 巴斯夫欧洲公司 具有改变的RemA/RemB蛋白的改良芽孢杆菌宿主细胞
WO2023117970A1 (en) 2021-12-20 2023-06-29 Basf Se Method for improved production of intracellular proteins in bacillus

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Publication number Publication date
EP3947656A1 (en) 2022-02-09
CA3136113A1 (en) 2020-10-08
US20220162621A1 (en) 2022-05-26
KR20210148270A (ko) 2021-12-07
WO2020206197A1 (en) 2020-10-08
JP2022526414A (ja) 2022-05-24

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