MX2021012158A - Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos. - Google Patents

Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos.

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Publication number
MX2021012158A
MX2021012158A MX2021012158A MX2021012158A MX2021012158A MX 2021012158 A MX2021012158 A MX 2021012158A MX 2021012158 A MX2021012158 A MX 2021012158A MX 2021012158 A MX2021012158 A MX 2021012158A MX 2021012158 A MX2021012158 A MX 2021012158A
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MX
Mexico
Prior art keywords
bacillus
genome
integrating
methods
compositions
Prior art date
Application number
MX2021012158A
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English (en)
Inventor
Wonchul Suh
Ryan L Frisch
Stacey Irene Robida Stubbs
Derek Joseph Zimmer
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Danisco Us Inc
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Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2021012158A publication Critical patent/MX2021012158A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
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  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se proporcionan métodos y composiciones para la integración de secuencias de ADN donante en el genoma de una célula de Bacillus sp. sin la integración de un marcador seleccionable en el genoma. Los métodos emplean un constructo de ADN recombinante lineal que comprende un ADN donante flanqueado por brazos de homología largos (cada uno de al menos 1000 nucleótidos de longitud) en combinación con un constructo de ADN recombinante que codifica una endonucleasa Cas9 y un ARN guía, para la introducción de un sistema de ARN guía/endonucleasa Cas (RGEN) en una célula de Bacillus sp., y como tal proporciona un sistema altamente eficaz para la integración de secuencias de ADN donante en el genoma de la célula de Bacillus sp., sin la necesidad de integrar un marcador seleccionable en el genoma de la célula de Bacillus sp.
MX2021012158A 2019-04-05 2020-04-03 Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos. MX2021012158A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962829662P 2019-04-05 2019-04-05
PCT/US2020/026508 WO2020206202A1 (en) 2019-04-05 2020-04-03 Methods for integrating a donor dna sequence into the genome of bacillus using linear recombinant dna constructs and compositions thereof

Publications (1)

Publication Number Publication Date
MX2021012158A true MX2021012158A (es) 2022-01-06

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MX2021012158A MX2021012158A (es) 2019-04-05 2020-04-03 Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos.

Country Status (7)

Country Link
US (1) US20220177923A1 (es)
EP (1) EP3947662A1 (es)
JP (1) JP2022526982A (es)
KR (1) KR20210148269A (es)
CA (1) CA3136114A1 (es)
MX (1) MX2021012158A (es)
WO (1) WO2020206202A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112023027009A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse
EP4359424A1 (en) 2021-06-24 2024-05-01 Basf Se Improved bacillus production host
WO2022269084A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus host cell with altered rema/remb protein
WO2023117970A1 (en) 2021-12-20 2023-06-29 Basf Se Method for improved production of intracellular proteins in bacillus

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KR20210148269A (ko) 2021-12-07
EP3947662A1 (en) 2022-02-09
WO2020206202A1 (en) 2020-10-08
CA3136114A1 (en) 2020-10-08
JP2022526982A (ja) 2022-05-27
US20220177923A1 (en) 2022-06-09

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