MX2021003995A - Plasmido que contiene una secuencia que codifica un arnm con una cola de poli(a) segmentada. - Google Patents

Plasmido que contiene una secuencia que codifica un arnm con una cola de poli(a) segmentada.

Info

Publication number
MX2021003995A
MX2021003995A MX2021003995A MX2021003995A MX2021003995A MX 2021003995 A MX2021003995 A MX 2021003995A MX 2021003995 A MX2021003995 A MX 2021003995A MX 2021003995 A MX2021003995 A MX 2021003995A MX 2021003995 A MX2021003995 A MX 2021003995A
Authority
MX
Mexico
Prior art keywords
tail
elements
nucleotide
nucleotides
mrna
Prior art date
Application number
MX2021003995A
Other languages
English (en)
Inventor
Zeljka Trepotec
Manish Kumar Aneja
Carsten Rudolfph
Christian Plank
Ludwing WEISS
Original Assignee
Ethris Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ethris Gmbh filed Critical Ethris Gmbh
Publication of MX2021003995A publication Critical patent/MX2021003995A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/50—Vectors for producing vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente divulgación proporciona un plásmido de ADN que comprende una secuencia que codifica una molécula de ARNm y una cola de poli(A) modificada, en el que la parte de la secuencia que codifica la cola de poli(A) modificada se caracteriza porque ésta consiste de al menos dos elementos A cada uno definido como una secuencia de nucleótidos que consiste en de 55 a 65 nucleótidos T y al menos un elemento S cada elemento S consiste en un nucleótido que no es un nucleótido T, o 2 a 10 nucleótidos, preferiblemente 6 nucleótidos, en el que cada uno de los dos nucleótidos terminales no es un nucleótido T, en el que el número de elementos A es uno más que el número de elementos S, y en el que dos elementos A cualesquiera están separados por un elemento S; exhibiendo dicho plásmido de ADN una recombinación reducida durante la amplificación en una célula huésped bacteriana en comparación con el mismo plásmido de ADN sin dicho al menos un elemento S.
MX2021003995A 2018-10-11 2019-10-10 Plasmido que contiene una secuencia que codifica un arnm con una cola de poli(a) segmentada. MX2021003995A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18199857 2018-10-11
PCT/EP2019/077477 WO2020074642A1 (en) 2018-10-11 2019-10-10 Plasmid containing a sequence encoding an mrna with a segmented poly(a) tail

Publications (1)

Publication Number Publication Date
MX2021003995A true MX2021003995A (es) 2021-06-23

Family

ID=64023934

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021003995A MX2021003995A (es) 2018-10-11 2019-10-10 Plasmido que contiene una secuencia que codifica un arnm con una cola de poli(a) segmentada.

Country Status (15)

Country Link
US (1) US20210348172A1 (es)
EP (2) EP3864156B1 (es)
JP (1) JP2022504663A (es)
KR (1) KR20210075102A (es)
CN (1) CN112805386A (es)
AU (1) AU2019358238A1 (es)
BR (1) BR112021006597A2 (es)
CA (1) CA3115597A1 (es)
DK (1) DK3864156T3 (es)
ES (1) ES2958832T3 (es)
HU (1) HUE063060T2 (es)
MX (1) MX2021003995A (es)
PL (1) PL3864156T3 (es)
PT (1) PT3864156T (es)
WO (1) WO2020074642A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202015009974U1 (de) 2014-12-12 2022-02-17 Curevac Ag Artifizielle Nukleinsäuremoleküle für eine verbesserte Proteinexpression

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030083272A1 (en) * 1997-09-19 2003-05-01 Lahive & Cockfield, Llp Sense mrna therapy
KR20120097484A (ko) 2009-07-31 2012-09-04 에트리스 게엠베하 단백질 발현을 위한 비변형된 뉴클레오티드 및 변형된 뉴클레오티드의 조합을 가진 ｒｎａ
WO2016005004A1 (en) * 2014-07-11 2016-01-14 Biontech Rna Pharmaceuticals Gmbh Stabilization of poly(a) sequence encoding dna sequences
EP3112469B1 (en) * 2015-06-30 2018-02-07 ethris GmbH Utrs increasing the translation efficiency of rna molecules
CN105505755A (zh) * 2015-12-23 2016-04-20 杭州谷禾信息技术有限公司 空间转录组建库测序方法及所用装置
CN105734053B (zh) * 2016-04-20 2018-12-14 武汉生命之美科技有限公司 一种高通量测序分析Poly(A)尾长度文库的构建方法

Also Published As

Publication number Publication date
DK3864156T3 (da) 2023-10-09
EP3864156A1 (en) 2021-08-18
WO2020074642A1 (en) 2020-04-16
AU2019358238A1 (en) 2021-05-06
HUE063060T2 (hu) 2023-12-28
PL3864156T3 (pl) 2024-01-03
CN112805386A (zh) 2021-05-14
KR20210075102A (ko) 2021-06-22
CA3115597A1 (en) 2020-04-16
EP4306643A2 (en) 2024-01-17
ES2958832T3 (es) 2024-02-15
BR112021006597A2 (pt) 2021-07-20
EP3864156B1 (en) 2023-08-16
EP4306643A3 (en) 2024-03-20
PT3864156T (pt) 2023-10-04
US20210348172A1 (en) 2021-11-11
JP2022504663A (ja) 2022-01-13

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