ES2543203T3 - Grupo de genes NRPS-PKS, su manipulación y su utilidad - Google Patents

Grupo de genes NRPS-PKS, su manipulación y su utilidad Download PDF

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Publication number
ES2543203T3
ES2543203T3 ES09723397.7T ES09723397T ES2543203T3 ES 2543203 T3 ES2543203 T3 ES 2543203T3 ES 09723397 T ES09723397 T ES 09723397T ES 2543203 T3 ES2543203 T3 ES 2543203T3
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Spain
Prior art keywords
nucleotide sequence
seq
sequence
nrps
usefulness
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Active
Application number
ES09723397.7T
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English (en)
Inventor
Sergey Zotchev
Hanne JØRGENSEN
Havard Sletta
Trond Erling Ellingsen
Espen Fjaervik
Kristin Fløgstad Degnes
Geir Klinkenberg
Per Bruheim
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Sintef Tto AS
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Sintef Tto AS
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Publication of ES2543203T3 publication Critical patent/ES2543203T3/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D225/00—Heterocyclic compounds containing rings of more than seven members having one nitrogen atom as the only ring hetero atom
    • C07D225/02—Heterocyclic compounds containing rings of more than seven members having one nitrogen atom as the only ring hetero atom not condensed with other rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Other In-Based Heterocyclic Compounds (AREA)

Abstract

Una molécula de ácido nucleico que comprende: (a) una secuencia de nucleótidos como se muestra en la SEC ID Nº 1; o (b) una secuencia de nucleótidos que es el complemento de la SEC ID Nº 1; o (c) una secuencia de nucleótidos que tiene una identidad de secuencia de al menos 85 % (preferentemente una identidad de secuencia de al menos 87 %, 90 %, 91 %, 92 %, 93 %, 94 %, 95%, 96 %, 97 %, 98 % o 99 %) con la SEC ID Nº 1; o (d) una parte de una cualquiera de (a) a (c), en la que dicha parte comprende: (i) una secuencia de nucleótidos como se muestra en una cualquiera de las SEC ID Nº 2, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 3 8, 40 o 44 o una secuencia de nucleótidos que es complementaria de las mismas o que tiene una identidad de secuencia de al menos 85 % con las mismas; o (ii) una secuencia de nucleótidos que codifica una o más secuencias de aminoácidos seleccionadas de SEC ID Nº 3, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41 o 45 o que tiene una identidad de secuencia de al menos 85 % (preferentemente al menos 90 %, 91 %, 92 %, 93 %, 94 %, 95 %, 96 %, 97 %, 98 % o 99 %) con las mismas, en la que dicha molécula de ácido nucleico codificaones complementaria a una molécula de ácido nucleico que codifica uno o más polipéptidos que tienen actividad funcional en la síntesis de BE-14106.
ES09723397.7T 2008-03-20 2009-03-20 Grupo de genes NRPS-PKS, su manipulación y su utilidad Active ES2543203T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0805363.9A GB0805363D0 (en) 2008-03-20 2008-03-20 Novel NRPS-PKS gene cluster and its manipulation and utility
GB0805363 2008-03-20
PCT/GB2009/000759 WO2009115822A1 (en) 2008-03-20 2009-03-20 Nrps-pks gene cluster and its manipulation and utility

Publications (1)

Publication Number Publication Date
ES2543203T3 true ES2543203T3 (es) 2015-08-17

Family

ID=39386664

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09723397.7T Active ES2543203T3 (es) 2008-03-20 2009-03-20 Grupo de genes NRPS-PKS, su manipulación y su utilidad

Country Status (10)

Country Link
US (2) US9217150B2 (es)
EP (1) EP2271666B1 (es)
JP (1) JP5514185B2 (es)
CN (1) CN102015756B (es)
AU (1) AU2009227740B2 (es)
CA (1) CA2718429C (es)
DK (1) DK2271666T3 (es)
ES (1) ES2543203T3 (es)
GB (1) GB0805363D0 (es)
WO (1) WO2009115822A1 (es)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9428845B1 (en) 2010-12-28 2016-08-30 Warp Drive Bio, Inc. Identifying new therapeutic agents
EP4289950A3 (en) 2015-01-09 2024-01-24 Revolution Medicines, Inc. Macrocyclic compounds that participate in cooperative binding and medical uses thereof
EP3331996A1 (en) * 2015-08-05 2018-06-13 Merck Patent GmbH Artificial non-ribosomal peptide synthetases
AU2016329064B2 (en) 2015-10-01 2023-10-19 Warp Drive Bio, Inc. Methods and reagents for analyzing protein-protein interfaces
CN106916834B (zh) * 2015-12-24 2022-08-05 武汉合生科技有限公司 化合物的生物合成基因簇及其应用
EP3442599B1 (en) 2016-04-12 2022-03-30 Warp Drive Bio, Inc. Compositions and methods for the production of compounds
US11071302B2 (en) 2016-09-30 2021-07-27 Aphea.Bio Nv Means and methods for plant yield enhancement
US11479797B2 (en) 2016-10-28 2022-10-25 Ginkgo Bioworks, Inc. Compositions and methods for the production of compounds
CN111778222B (zh) * 2019-04-04 2024-03-22 中国科学院微生物研究所 一个能在真菌中产生黄酮类化合物的nrps-pks杂合蛋白及其编码基因与应用
JP2022553859A (ja) 2019-11-04 2022-12-26 レボリューション メディシンズ インコーポレイテッド Ｒａｓ阻害剤
CA3159561A1 (en) 2019-11-04 2021-05-14 Revolution Medicines, Inc. Ras inhibitors
WO2021091967A1 (en) 2019-11-04 2021-05-14 Revolution Medicines, Inc. Ras inhibitors
CA3194067A1 (en) 2020-09-15 2022-03-24 Revolution Medicines, Inc. Ras inhibitors
CN113278601B (zh) * 2021-05-17 2022-07-08 浙江工业大学 一种腺苷酰化蛋白a6突变体及其编码基因与应用
CN114657155B (zh) * 2021-12-23 2024-01-19 中国农业科学院生物技术研究所 一种新型的非核糖体多肽合成酶拆分表达方法
GB202207185D0 (en) 2022-05-17 2022-06-29 Sintef Tto As Novel antibiotic compound
CN116064614A (zh) * 2022-09-16 2023-05-05 中国科学院深海科学与工程研究所 特特拉姆酸类化合物的生物合成基因簇及应用
CN117551667B (zh) * 2024-01-03 2024-03-12 中国农业科学院农业基因组研究所 Rbb1基因在制备水稻类病斑材料和调控水稻抗病菌感染能力中的应用
CN117925744B (zh) * 2024-01-15 2024-07-23 苏州华赛生物工程技术有限公司 非核糖体肽合成酶在生产脱羧肌肽中的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH041179A (ja) 1990-04-17 1992-01-06 Banyu Pharmaceut Co Ltd 抗腫瘍性物質ｂｅ―１４１０６
EP1255840A2 (en) * 2000-02-08 2002-11-13 Norges Teknisk Naturvitenskapelige Universitet Gene cluster encoding a nystatin polyketide synthase and its manipulation and utility
MXPA02010565A (es) * 2000-04-28 2004-05-17 Kosan Biosciences Inc Produccion de policetidos.
CA2484638C (en) * 2003-09-11 2008-03-25 Ecopia Biosciences Inc. Polyene polyketides and methods of production
JP4001179B2 (ja) * 2005-12-21 2007-10-31 日立電線株式会社 レベルワウンドコイルとその製造方法、およびレベルワウンドコイルの包装体

Also Published As

Publication number Publication date
EP2271666B1 (en) 2015-05-06
EP2271666A1 (en) 2011-01-12
JP5514185B2 (ja) 2014-06-04
GB0805363D0 (en) 2008-04-30
JP2011515086A (ja) 2011-05-19
US20110117606A1 (en) 2011-05-19
AU2009227740A1 (en) 2009-09-24
AU2009227740B2 (en) 2014-02-13
CA2718429A1 (en) 2009-09-24
CA2718429C (en) 2019-09-24
US20160060636A1 (en) 2016-03-03
CN102015756B (zh) 2014-07-30
DK2271666T3 (da) 2015-07-20
CN102015756A (zh) 2011-04-13
WO2009115822A1 (en) 2009-09-24
US9217150B2 (en) 2015-12-22
US10047363B2 (en) 2018-08-14

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