MX2020005724A - Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. - Google Patents

Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.

Info

Publication number
MX2020005724A
MX2020005724A MX2020005724A MX2020005724A MX2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A
Authority
MX
Mexico
Prior art keywords
seq
alpha
polynucleotides encoding
variants
amylase variants
Prior art date
Application number
MX2020005724A
Other languages
English (en)
Inventor
Carsten Andersen
Louis Patrick Lessard
Lars Giger
Jensen Johanne Mørch
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2020005724A publication Critical patent/MX2020005724A/es

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)

Abstract

La presente invención se refiere a variantes de alfa-amilasa que comprenden sustituciones en las posiciones correspondientes a las posiciones 268 y 293 de SEC. ID NO.: 1, en particular, sustituciones seleccionadas del grupo que consiste en: 268G+293Y; 268G+293F; 268G+293W; 268G+293H; 268G+293A; 268G+293Q; 268A+293Y; 268A+293F; 268A+293W; 268A+293H; 268A+293A; 268A+293Q; 268P+293Y; 268P+293F; 268P+293W; 268P+293H; 268P+293A; 268P+293Q; 268S+293Y; 268S+293F; 268S+293W; 268S+293H; 268S+293A; 268S+293Q; 268T+293Y; 268T+293F; 268T+293W; 268T+293H; 268T+293A; 268T+293Q; 268V+293Y; 268V+293F; 268V+293W; 268V+293H; 268V+293A; 268V+293Q; 268I+293Y; 268I+293F; 268I+293W; 268I+293H; 268I+293A; 268I+293Q; 268L+293Y; 268L+293F; 268L+293W; 268L+293H; 268L+293A; 268L+293Q; 268M+293Y; 268M+293F; 268M+293W; 268M+293H; 268M+293A; 268M+293Q;y donde la variante tiene al menos el 70 %, al menos el 75 %, al menos el 80 %, al menos el 85 %, al menos el 90 %, al menos el 95 %, al menos el 96 %, al menos el 97 %, al menos el 98 % o al menos el 99 %, pero menos del 100 % de identidad de secuencia con una alfa-amilasa precursora seleccionada del grupo que consiste en SEC. ID NO.: 1, SEC. ID NO.: 2, SEC. ID NO.: 3, SEC. ID NO.: 4 y SEC. ID NO.: 18. La presente invención también se refiere apolinucleótidos que codifican las variantes; a construcciones de ácido nucleico, vectores y células hospedadoras que comprenden los polinucleótidos; y a métodos para usar las variantes.
MX2020005724A 2017-12-08 2018-12-07 Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. MX2020005724A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762596231P 2017-12-08 2017-12-08
US201862765268P 2018-08-20 2018-08-20
PCT/US2018/064415 WO2019113415A1 (en) 2017-12-08 2018-12-07 Alpha-amylase variants and polynucleotides encoding same

Publications (1)

Publication Number Publication Date
MX2020005724A true MX2020005724A (es) 2020-08-13

Family

ID=64746663

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020005724A MX2020005724A (es) 2017-12-08 2018-12-07 Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.

Country Status (10)

Country Link
US (1) US11384347B2 (es)
EP (1) EP3720956B1 (es)
CN (1) CN111492054A (es)
BR (1) BR112020011173A2 (es)
CA (1) CA3081096A1 (es)
DK (1) DK3720956T5 (es)
ES (1) ES2954431T3 (es)
FI (1) FI3720956T3 (es)
MX (1) MX2020005724A (es)
WO (1) WO2019113415A1 (es)

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WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast

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Also Published As

Publication number Publication date
EP3720956B1 (en) 2023-06-07
US20200392474A1 (en) 2020-12-17
EP3720956A1 (en) 2020-10-14
WO2019113415A1 (en) 2019-06-13
FI3720956T3 (fi) 2023-08-11
CA3081096A1 (en) 2019-06-13
ES2954431T3 (es) 2023-11-22
DK3720956T3 (da) 2023-09-04
BR112020011173A2 (pt) 2020-11-17
DK3720956T5 (da) 2024-08-26
US11384347B2 (en) 2022-07-12
CN111492054A (zh) 2020-08-04

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