MX2020005724A - Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. - Google Patents
Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.Info
- Publication number
- MX2020005724A MX2020005724A MX2020005724A MX2020005724A MX2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A MX 2020005724 A MX2020005724 A MX 2020005724A
- Authority
- MX
- Mexico
- Prior art keywords
- seq
- alpha
- polynucleotides encoding
- variants
- amylase variants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Detergent Compositions (AREA)
Abstract
La presente invención se refiere a variantes de alfa-amilasa que comprenden sustituciones en las posiciones correspondientes a las posiciones 268 y 293 de SEC. ID NO.: 1, en particular, sustituciones seleccionadas del grupo que consiste en: 268G+293Y; 268G+293F; 268G+293W; 268G+293H; 268G+293A; 268G+293Q; 268A+293Y; 268A+293F; 268A+293W; 268A+293H; 268A+293A; 268A+293Q; 268P+293Y; 268P+293F; 268P+293W; 268P+293H; 268P+293A; 268P+293Q; 268S+293Y; 268S+293F; 268S+293W; 268S+293H; 268S+293A; 268S+293Q; 268T+293Y; 268T+293F; 268T+293W; 268T+293H; 268T+293A; 268T+293Q; 268V+293Y; 268V+293F; 268V+293W; 268V+293H; 268V+293A; 268V+293Q; 268I+293Y; 268I+293F; 268I+293W; 268I+293H; 268I+293A; 268I+293Q; 268L+293Y; 268L+293F; 268L+293W; 268L+293H; 268L+293A; 268L+293Q; 268M+293Y; 268M+293F; 268M+293W; 268M+293H; 268M+293A; 268M+293Q;y donde la variante tiene al menos el 70 %, al menos el 75 %, al menos el 80 %, al menos el 85 %, al menos el 90 %, al menos el 95 %, al menos el 96 %, al menos el 97 %, al menos el 98 % o al menos el 99 %, pero menos del 100 % de identidad de secuencia con una alfa-amilasa precursora seleccionada del grupo que consiste en SEC. ID NO.: 1, SEC. ID NO.: 2, SEC. ID NO.: 3, SEC. ID NO.: 4 y SEC. ID NO.: 18. La presente invención también se refiere apolinucleótidos que codifican las variantes; a construcciones de ácido nucleico, vectores y células hospedadoras que comprenden los polinucleótidos; y a métodos para usar las variantes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762596231P | 2017-12-08 | 2017-12-08 | |
US201862765268P | 2018-08-20 | 2018-08-20 | |
PCT/US2018/064415 WO2019113415A1 (en) | 2017-12-08 | 2018-12-07 | Alpha-amylase variants and polynucleotides encoding same |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2020005724A true MX2020005724A (es) | 2020-08-13 |
Family
ID=64746663
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2020005724A MX2020005724A (es) | 2017-12-08 | 2018-12-07 | Variantes de alfa-amilasa y polinucleotidos que codifican las mismas. |
Country Status (10)
Country | Link |
---|---|
US (1) | US11384347B2 (es) |
EP (1) | EP3720956B1 (es) |
CN (1) | CN111492054A (es) |
BR (1) | BR112020011173A2 (es) |
CA (1) | CA3081096A1 (es) |
DK (1) | DK3720956T5 (es) |
ES (1) | ES2954431T3 (es) |
FI (1) | FI3720956T3 (es) |
MX (1) | MX2020005724A (es) |
WO (1) | WO2019113415A1 (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
WO2024137246A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same |
WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
WO2024137250A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
WO2024137704A2 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast |
Family Cites Families (40)
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US3912590A (en) | 1973-01-03 | 1975-10-14 | Novo Industri As | Procedure for liquefying starch |
US4316956A (en) | 1980-02-06 | 1982-02-23 | Novo Industri A/S | Fermentation process |
US4335208A (en) | 1980-03-11 | 1982-06-15 | Novo Industri A/S | Saccharification of starch hydrolysates |
JPS57174089A (en) | 1981-04-20 | 1982-10-26 | Novo Industri As | Chain dividing enzyme product |
US4560651A (en) | 1981-04-20 | 1985-12-24 | Novo Industri A/S | Debranching enzyme product, preparation and use thereof |
ATE80657T1 (de) | 1986-07-09 | 1992-10-15 | Novo Nordisk As | Mischungen von alpha-amylase zur verfluessigung von staerke. |
US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
AU641169B2 (en) | 1989-06-13 | 1993-09-16 | Genencor International, Inc. | A method for killing cells without cell lysis |
IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
US5231017A (en) | 1991-05-17 | 1993-07-27 | Solvay Enzymes, Inc. | Process for producing ethanol |
FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
EP0994191B1 (en) | 1997-06-10 | 2006-03-01 | Takara Bio Inc. | System for expressing hyperthermostable protease |
AU9434398A (en) | 1997-10-13 | 1999-05-03 | Novo Nordisk A/S | Alpha-amylase mutants |
US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
EP1818396B1 (en) | 1999-03-30 | 2014-06-18 | Novozymes A/S | Alpha-amylase variants |
EP1250423B1 (en) | 2000-01-12 | 2008-09-03 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
ATE375388T1 (de) | 2001-07-27 | 2007-10-15 | Us Gov Health & Human Serv | Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden |
RU2318018C2 (ru) | 2001-12-07 | 2008-02-27 | Новозимс А/С | Полипептиды, обладающие протеазной активностью, и нуклеиновые кислоты, кодирующие указанные полипептиды |
WO2009061381A2 (en) | 2007-11-05 | 2009-05-14 | Danisco Us Inc., Genencor Division | Alpha-amylase variants with altered properties |
DK2447361T3 (en) * | 2008-06-06 | 2015-01-05 | Danisco Us Inc | Alpha-amylase (AMYS) variants of Geobacillus stearothermophilus with improved properties |
CA2726688A1 (en) | 2008-06-23 | 2010-01-21 | Novozymes A/S | Processes for producing fermentation products |
WO2010036515A1 (en) | 2008-09-25 | 2010-04-01 | Danisco Us Inc. | Alpha-amylase blends and methods for using said blends |
BRPI1008890A2 (pt) | 2009-02-20 | 2015-08-25 | Danisco Us Inc | Formulações de caldos de fermentação |
BRPI1010238A2 (pt) | 2009-04-01 | 2015-08-25 | Danisco Us Inc | Composições e métodos que comprendem variantes de alfa-amilase com propriedades alteradas |
MX2012006096A (es) | 2009-11-30 | 2012-07-03 | Novozymes North America Inc | Polipeptidos que tienen actividad glucoamilasa y polinucleotidos que codifican para los mismos. |
CA2782036C (en) | 2009-12-01 | 2019-01-15 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
CN103068975B (zh) | 2010-01-04 | 2018-03-20 | 诺维信公司 | α‑淀粉酶变体和编码该变体的多核苷酸 |
DK2558484T3 (en) | 2010-04-14 | 2016-03-29 | Novozymes As | Polypeptides having glucoamylase activity and polynucleotides encoding them |
CN103298932B (zh) | 2010-11-08 | 2016-08-10 | 诺维信公司 | 具有葡糖淀粉酶活性的多肽和编码该多肽的多核苷酸 |
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KR102046075B1 (ko) | 2011-10-17 | 2019-11-18 | 노보자임스 에이/에스 | 알파-아밀라아제 변이체 및 이를 암호화하는 폴리뉴클레오티드 |
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WO2015050723A1 (en) | 2013-10-03 | 2015-04-09 | Danisco Us Inc. | Alpha-amylases from exiguobacterium, and methods of use, thereof |
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-
2018
- 2018-12-07 DK DK18822573.4T patent/DK3720956T5/da active
- 2018-12-07 BR BR112020011173-2A patent/BR112020011173A2/pt unknown
- 2018-12-07 WO PCT/US2018/064415 patent/WO2019113415A1/en unknown
- 2018-12-07 CA CA3081096A patent/CA3081096A1/en active Pending
- 2018-12-07 EP EP18822573.4A patent/EP3720956B1/en active Active
- 2018-12-07 US US16/770,853 patent/US11384347B2/en active Active
- 2018-12-07 CN CN201880078038.7A patent/CN111492054A/zh active Pending
- 2018-12-07 MX MX2020005724A patent/MX2020005724A/es unknown
- 2018-12-07 FI FIEP18822573.4T patent/FI3720956T3/fi active
- 2018-12-07 ES ES18822573T patent/ES2954431T3/es active Active
Also Published As
Publication number | Publication date |
---|---|
EP3720956B1 (en) | 2023-06-07 |
US20200392474A1 (en) | 2020-12-17 |
EP3720956A1 (en) | 2020-10-14 |
WO2019113415A1 (en) | 2019-06-13 |
FI3720956T3 (fi) | 2023-08-11 |
CA3081096A1 (en) | 2019-06-13 |
ES2954431T3 (es) | 2023-11-22 |
DK3720956T3 (da) | 2023-09-04 |
BR112020011173A2 (pt) | 2020-11-17 |
DK3720956T5 (da) | 2024-08-26 |
US11384347B2 (en) | 2022-07-12 |
CN111492054A (zh) | 2020-08-04 |
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