BR112014027468A2 - polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo. - Google Patents

polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.

Info

Publication number
BR112014027468A2
BR112014027468A2 BR112014027468A BR112014027468A BR112014027468A2 BR 112014027468 A2 BR112014027468 A2 BR 112014027468A2 BR 112014027468 A BR112014027468 A BR 112014027468A BR 112014027468 A BR112014027468 A BR 112014027468A BR 112014027468 A2 BR112014027468 A2 BR 112014027468A2
Authority
BR
Brazil
Prior art keywords
polynucleotide
plant
isolated
recombinant
disruption
Prior art date
Application number
BR112014027468A
Other languages
English (en)
Inventor
Mark Cigan Andrew
ONG Azalea
Bermudez Ericka
Gao Huirong
English James
K Young Joshua
Liu Lu
Carl Falco Saverio
Svitashev Sergei
Bin Liu Zhan
Original Assignee
Du Pont
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int filed Critical Du Pont
Publication of BR112014027468A2 publication Critical patent/BR112014027468A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
BR112014027468A 2012-05-04 2013-05-01 polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo. BR112014027468A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261642470P 2012-05-04 2012-05-04
US201261683765P 2012-08-16 2012-08-16
PCT/US2013/039011 WO2013166113A1 (en) 2012-05-04 2013-05-01 Compositions and methods comprising sequences having meganuclease activity

Publications (1)

Publication Number Publication Date
BR112014027468A2 true BR112014027468A2 (pt) 2017-06-27

Family

ID=48326496

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014027468A BR112014027468A2 (pt) 2012-05-04 2013-05-01 polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.

Country Status (6)

Country Link
US (5) US20150184173A1 (pt)
CN (1) CN104411823A (pt)
BR (1) BR112014027468A2 (pt)
CA (1) CA2872096A1 (pt)
RU (1) RU2014148769A (pt)
WO (1) WO2013166113A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019050902A1 (en) * 2017-09-05 2019-03-14 Adaptive Phage Therapeutics, Inc. METHODS OF DETERMINING THE SENSITIVITY PROFILE OF A BACTERIAL STRAIN TO A THERAPEUTIC COMPOSITION
US11071816B2 (en) 2017-10-04 2021-07-27 Johnson & Johnson Surgical Vision, Inc. System, apparatus and method for monitoring anterior chamber intraoperative intraocular pressure
EP3691585B1 (en) 2017-10-04 2023-09-27 Johnson & Johnson Surgical Vision, Inc. Systems for measuring fluid flow in a venturi based system
US11969380B2 (en) 2017-10-04 2024-04-30 Johnson & Johnson Surgical Vision, Inc. Advanced occlusion management methods for a phacoemulsification system
EP3691707B1 (en) 2017-10-04 2021-11-17 Johnson & Johnson Surgical Vision, Inc. A system to augment irrigation pressure and to maintain iop during post occlusion surge
US20210324398A1 (en) 2018-06-29 2021-10-21 Pioneer Hi-Bred International, Inc. Edited nac genes in plants
KR20220005555A (ko) * 2019-05-07 2022-01-13 프리시젼 바이오사이언시스 인코포레이티드 인식 서열에 대한 조작된 메가뉴클레아제의 최적화
WO2024036190A2 (en) 2022-08-09 2024-02-15 Pioneer Hi-Bred International, Inc. Guide polynucleotide multiplexing
WO2024123786A1 (en) 2022-12-06 2024-06-13 Pioneer Hi-Bred International, Inc. Methods and compositions for co-delivery of t-dnas expressing multiple guide polynucleotides into plants

Family Cites Families (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
ES2078518T3 (es) 1990-04-05 1995-12-16 Roberto Crea Mutagenesis por desplazamiento completo.
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
HU219057B (hu) 1991-08-27 2001-02-28 Novartis Ag. Inszekticid hatású proteinek Homoptera rovarok ellen és alkalmazásuk a növényvédelemben
TW261517B (pt) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
CA2219136A1 (en) 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US5756316A (en) 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5783431A (en) 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
JP2001504325A (ja) 1996-09-27 2001-04-03 マキシジェン，インコーポレイテッド 回帰的配列シャフリングおよび選択による遺伝子治療の最適化のための方法
DE69835360T2 (de) 1997-01-17 2007-08-16 Maxygen, Inc., Redwood City EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
EP0996718B1 (en) 1997-03-18 2008-12-24 Novozymes A/S Method for constructing a library using dna shuffling
BR9808368A (pt) 1997-03-18 2000-05-23 Novo Nordisk As Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse.
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
WO1999021979A1 (en) 1997-10-28 1999-05-06 Maxygen, Inc. Human papillomavirus vectors
EP1690868A1 (en) 1997-10-31 2006-08-16 Maxygen, Inc. Modification of virus tropism and host range by viral genome shuffling
DE69833457T2 (de) 1997-11-18 2006-09-14 Pioneer Hi-Bred International, Inc. Neuartige methode zur integration von fremd-dna ins eukaryotische genom
EP1032692A1 (en) 1997-11-18 2000-09-06 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
WO1999025855A1 (en) 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems
AU760113C (en) 1997-11-18 2004-04-22 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
AU3291099A (en) 1998-02-11 1999-08-30 Maxygen, Inc. Genetic vaccine vector engineering
AU3289199A (en) 1998-02-11 1999-08-30 Maxygen, Inc. Antigen library immunization
AU762993C (en) 1998-02-26 2004-06-10 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
IL140100A0 (en) 1998-06-29 2002-02-10 Phylos Inc Methods of generating highly diverse libraries
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
KR20010085850A (ko) 1998-09-29 2001-09-07 추후제출 코돈 변형 유전자의 재편성
IL142736A0 (en) 1998-11-09 2002-03-10 Pioneer Hi Bred Int Transcriptional activator lec 1 nucleic acids, polypeptides and their uses
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
AU2415200A (en) 1999-01-18 2000-08-01 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
EP1072010B1 (en) 1999-01-19 2010-04-21 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
DE60316124T3 (de) 2002-03-15 2018-03-22 Cellectis Hybride and einzelkettige meganukleasen und deren anwendungen
AU2003290518A1 (en) 2002-09-06 2004-04-23 Fred Hutchinson Cancer Research Center Methods and compositions concerning designed highly-specific nucleic acid binding proteins
AU2005210493B2 (en) 2004-02-02 2009-06-04 Pioneer Hi-Bred International, Inc. AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use
AU2005216528B2 (en) 2004-02-25 2009-06-04 Pioneer Hi-Bred International, Inc. Novel Bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
EP1591521A1 (en) 2004-04-30 2005-11-02 Cellectis I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof
WO2006097784A1 (en) 2005-03-15 2006-09-21 Cellectis I-crei meganuclease variants with modified specificity, method of preparation and uses thereof
US20110158974A1 (en) 2005-03-15 2011-06-30 Cellectis Heterodimeric Meganucleases and Use Thereof
US8051717B2 (en) 2005-07-06 2011-11-08 Central Research Institute Of Electric Power Industry Method and apparatus for measuring flaw height in ultrasonic tests
JP5937292B2 (ja) * 2005-10-18 2016-06-22 デューク大学 配列特異性およびｄｎａ−結合親和度が変更された、合理設計メガヌクレアーゼ
EP2087120A2 (en) 2006-12-08 2009-08-12 Pioneer Hi-Bred International Inc. Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
WO2008102198A1 (en) * 2007-02-19 2008-08-28 Cellectis Laglidadg homing endonuclease variants having novel substrate specificity and use thereof
EP2568048A1 (en) * 2007-06-29 2013-03-13 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
US20100071983A1 (en) 2008-09-24 2010-03-25 Holland Ronald A Three wheel vehicle rear suspension
DK2215223T3 (da) * 2007-10-31 2013-07-22 Prec Biosciences Inc Rationelt konstruerede enkeltkæde-meganucleaser med ikke-palindrome genkendelsessekvenser
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
US20100071083A1 (en) * 2008-03-12 2010-03-18 Smith James J Temperature-dependent meganuclease activity
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
EP2529018B1 (en) 2009-12-30 2016-06-22 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
US9477260B2 (en) 2015-01-21 2016-10-25 Dell Products L.P. Systems and methods for coupling information handling resource disposed in information handling system bezel

Also Published As

Publication number Publication date
WO2013166113A1 (en) 2013-11-07
US20140223606A1 (en) 2014-08-07
CN104411823A (zh) 2015-03-11
CA2872096A1 (en) 2013-11-07
US20180142223A1 (en) 2018-05-24
US9499827B2 (en) 2016-11-22
US10150956B2 (en) 2018-12-11
US20170022488A1 (en) 2017-01-26
US20190040372A1 (en) 2019-02-07
RU2014148769A (ru) 2016-06-27
US9909110B2 (en) 2018-03-06
US20150184173A1 (en) 2015-07-02

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