BR112013029807A2 - polinucleotídeo recombatente, constructo de ácido nucleico, célula, planta, explante de planta, semente transgênica, polipeptídeo recombinante e método de direcionamento de um polipeptídeo de interesse a um cloroplasto - Google Patents

polinucleotídeo recombatente, constructo de ácido nucleico, célula, planta, explante de planta, semente transgênica, polipeptídeo recombinante e método de direcionamento de um polipeptídeo de interesse a um cloroplasto

Info

Publication number
BR112013029807A2
BR112013029807A2 BR112013029807A BR112013029807A BR112013029807A2 BR 112013029807 A2 BR112013029807 A2 BR 112013029807A2 BR 112013029807 A BR112013029807 A BR 112013029807A BR 112013029807 A BR112013029807 A BR 112013029807A BR 112013029807 A2 BR112013029807 A2 BR 112013029807A2
Authority
BR
Brazil
Prior art keywords
polypeptide
plant
recombinant
chloroplast
targeting
Prior art date
Application number
BR112013029807A
Other languages
English (en)
Inventor
Albert L Lu
Gary C Chun
Timothy M Bourett
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR112013029807A2 publication Critical patent/BR112013029807A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
BR112013029807A 2011-05-23 2012-05-10 polinucleotídeo recombatente, constructo de ácido nucleico, célula, planta, explante de planta, semente transgênica, polipeptídeo recombinante e método de direcionamento de um polipeptídeo de interesse a um cloroplasto BR112013029807A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161488952P 2011-05-23 2011-05-23
PCT/US2012/037360 WO2012161982A1 (en) 2011-05-23 2012-05-10 Chloroplast transit peptides and methods of their use

Publications (1)

Publication Number Publication Date
BR112013029807A2 true BR112013029807A2 (pt) 2017-01-24

Family

ID=46124770

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013029807A BR112013029807A2 (pt) 2011-05-23 2012-05-10 polinucleotídeo recombatente, constructo de ácido nucleico, célula, planta, explante de planta, semente transgênica, polipeptídeo recombinante e método de direcionamento de um polipeptídeo de interesse a um cloroplasto

Country Status (7)

Country Link
US (1) US9150625B2 (pt)
EP (1) EP2714725A1 (pt)
CN (1) CN103748108A (pt)
BR (1) BR112013029807A2 (pt)
CA (1) CA2836775A1 (pt)
MX (1) MX2013013644A (pt)
WO (1) WO2012161982A1 (pt)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX344968B (es) * 2012-02-01 2017-01-12 Dow Agrosciences Llc Peptido de transito al cloroplasto.
BR112015015055A2 (pt) 2012-12-21 2017-10-03 Pioneer Hi Bred Int Método para desintoxicar um herbicida análogo de auxina, método para controlar pelo menos uma erva em uma área de cultivo, método para testar uma resposta de planta a um ou mais compostos
WO2014150914A2 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
CN106232820A (zh) 2013-08-16 2016-12-14 先锋国际良种公司 杀昆虫蛋白及其使用方法
UA120598C2 (uk) 2013-09-13 2020-01-10 Піонір Хай-Бред Інтернешнл, Інк. Днк-конструкція та спосіб її застосування
WO2015120270A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Insecticidal proteins and methods for their use
CN106536545A (zh) 2014-02-07 2017-03-22 先锋国际良种公司 杀昆虫蛋白及其使用方法
UA126192C2 (uk) 2014-10-16 2022-08-31 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок та спосіб його застосування
BR112017019419B1 (pt) 2015-03-11 2022-10-11 E. I. Du Pont De Nemours And Company Construto de dna, método de obtenção de uma planta transgênica e método para controlar uma população de praga de verme da raiz do milho
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
UA124495C2 (uk) 2015-08-06 2021-09-29 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок рослинного походження та спосіб його застосування
CN108575091A (zh) 2015-12-18 2018-09-25 先锋国际良种公司 杀昆虫蛋白及其使用方法
WO2017192560A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3260542A1 (en) * 2016-06-20 2017-12-27 Algentech Protein production in plant cells
CN109788735A (zh) 2016-07-01 2019-05-21 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
KR102587506B1 (ko) 2016-07-29 2023-10-11 몬산토 테크놀로지 엘엘씨 식물에서 유전자 발현을 위한 방법 및 조성물
WO2018084936A1 (en) 2016-11-01 2018-05-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN110088123B (zh) 2016-12-14 2023-10-20 先锋国际良种公司 杀昆虫蛋白及其使用方法
WO2018118811A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3052794A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
EP3622076A1 (en) 2017-05-11 2020-03-18 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11761012B2 (en) 2017-11-03 2023-09-19 University Of Florida Research Foundation, Incorporated Mitigation of maize heat stress with recombinant 6-phosphogluconate dehydrogenase
JP6614622B2 (ja) * 2018-04-17 2019-12-04 国立大学法人名古屋大学 植物ゲノム編集方法
WO2020014483A1 (en) * 2018-07-11 2020-01-16 Cornell University Supported plant plasma membrane lipid bilayer on-a-chip
BR112021003797A2 (pt) 2018-08-29 2021-05-25 Pioneer Hi-Bred International, Inc. proteínas inseticidas e métodos para seu uso
CN111979208B (zh) * 2019-05-23 2023-01-10 弈柯莱生物科技(上海)股份有限公司 一种l-谷氨酸脱氢酶突变体及其应用
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
CN114085277B (zh) * 2022-01-19 2022-04-19 北京市农林科学院 一种将目的蛋白定位于叶绿体的方法及其应用
CN115851777B (zh) * 2022-08-22 2023-12-01 江西省超级水稻研究发展中心(江西省农科院海南水稻育种中心) 颖壳发育相关基因OsAPx5及其蛋白的应用

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ201918A (en) 1981-09-18 1987-04-30 Genentech Inc N-terminal methionyl analogues of bovine growth hormone
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
DE3587548T2 (de) 1984-12-28 1993-12-23 Bayer Ag Rekombinante DNA, die in pflanzliche Zellen eingebracht werden kann.
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
EP0463056A1 (en) 1989-03-17 1992-01-02 E.I. Du Pont De Nemours And Company External regulation of gene expression
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
DE69133128T2 (de) 1990-04-12 2003-06-18 Syngenta Participations Ag Gewebe-spezifische Promotoren
JP3387495B2 (ja) 1990-04-26 2003-03-17 プラント・ジエネテイツク・システムズ・エヌ・ベー 新規のＢａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ株及び殺虫毒素をコードするそれらの遺伝子
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
AU675628B2 (en) 1991-08-02 1997-02-13 Sumitomo Chemical Company, Limited Novel microorganism and insecticide
DE69230290T2 (de) 1991-08-27 2000-07-20 Novartis Ag Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
TW261517B (pt) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
JP2952041B2 (ja) 1992-07-27 1999-09-20 パイオニア ハイ−ブレッド インターナショナル，インコーポレイテッド 培養ダイズ細胞のａｇｒｏｂａｃｔｅｒｉｕｍ媒介形質転換の改良法
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5750868A (en) 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5737514A (en) 1995-11-29 1998-04-07 Texas Micro, Inc. Remote checkpoint memory system and protocol for fault-tolerant computer system
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
CA2309719A1 (en) 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Targeted manipulation of genes in plants
NZ504300A (en) 1997-11-18 2002-06-28 Pioneer Hi Bred Int A method for directional stable transformation of eukaryotic cells using non-identical recombination sites
AU745238C (en) 1997-11-18 2003-02-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from T-DNA using site-specific recombination systems
DE69833457T2 (de) 1997-11-18 2006-09-14 Pioneer Hi-Bred International, Inc. Neuartige methode zur integration von fremd-dna ins eukaryotische genom
AR014072A1 (es) 1998-02-26 2001-01-31 Pioneer Hi Bred Int Molecula de acido nucleico aislada que tiene una secuencia nucleotidica para un promotor que es capaz de iniciar una transcripcion constitutiva en unacelula de planta, construccion adn, vector, celula huesped, metodo para expresar en forma constitutiva una secuencia nucleotidica heteroloca en una
EP1104469B1 (en) 1998-08-20 2005-11-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
WO2000012733A1 (en) 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
US6489542B1 (en) 1998-11-04 2002-12-03 Monsanto Technology Llc Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids
WO2000028058A2 (en) 1998-11-09 2000-05-18 Pioneer Hi-Bred International, Inc. Transcriptional activator lec1 nucleic acids, polypeptides and their uses
EP1173582B1 (en) 1999-04-29 2006-06-14 Syngenta Limited Herbicide resistant plants
US6683231B2 (en) 2000-06-02 2004-01-27 E. I. Du Pont De Nemours And Company High level production of p-hydroxybenzoic acid in green plants
AU2001287862B2 (en) 2000-09-29 2006-12-14 Syngenta Limited Herbicide resistant plants
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
DE602005023727D1 (de) 2004-06-09 2010-11-04 Pioneer Hi Bred Int Plastiden transit peptide
JP5296684B2 (ja) 2006-08-11 2013-09-25 モンサント テクノロジー エルエルシー アントラニル酸合成酵素の葉緑体標的発現による高トリプトファントウモロコシの生産
US20110268865A1 (en) * 2008-08-01 2011-11-03 Bayer Bio Science N.V. Method for increasing photosynthetic carbon fixation in rice

Also Published As

Publication number Publication date
EP2714725A1 (en) 2014-04-09
CA2836775A1 (en) 2012-11-29
CN103748108A (zh) 2014-04-23
MX2013013644A (es) 2013-12-12
US20120304336A1 (en) 2012-11-29
WO2012161982A1 (en) 2012-11-29
US9150625B2 (en) 2015-10-06

Similar Documents

Publication Publication Date Title
BR112013029807A2 (pt) polinucleotídeo recombatente, constructo de ácido nucleico, célula, planta, explante de planta, semente transgênica, polipeptídeo recombinante e método de direcionamento de um polipeptídeo de interesse a um cloroplasto
BR112013003221A2 (pt) polinucleotídeo quimérico, construto de ácido nucleico, célula, planta, explante vegetal, semente transgênica, polipeptídeo quimérico, método de direcionar um polipeptídeo de interesse a um cloroplasto, cassete de expressão, célula vegetal, polipeptídeo isolado
CL2015000565A1 (es) Método de integración de transgenes por ingeniería genética para producir células de planta transgénica.
BR112013023148A2 (pt) molécula de ácido nucleico, construto de dna, célula hospedeira, planta transgênica, semente, pilopeptídeo isolado com atividade pesticida, composição, método para controlar uma população de pragas para exterminar uma praga, método para produzir um polipeptídeo com atividade pesticida, método para proteger uma planta contra uma praga
BR112014029908A2 (pt) método para aumentar a produção, taxa de crescimento, biomassa, vigor, teor de óleo, produção de sementes, produção de fibra, qualidade da fibra, resistência ao estresse abiótico e/ou eficiência no uso de nitrogênio de uma planta, método de produção de uma cultura, polinucleotídeo isolado, polipeptídeo isolado, estrutura de ácido nucléico, célula de planta, planta transgênica
EP2821486A4 (en) METHOD FOR INTRODUCING NUCLEIC ACID INTO PLANT CELLS
IL227552A0 (en) Nucleic acid construct for expression of alpha-galactosidase in plant cells
CL2013002707A1 (es) Construcción de adn que comprende un polinucleótido ligado operativamente a un ácido nucleico transcribible heterólogo, célula de planta transgénica que comprende dicha construcción, y método de expresión del ácido nucleico transcribible.
BR112014004130A2 (pt) polinucleotídeos e polipeptídeos isolados e métodos de uso dos mesmos para aumento da produção da planta e/ou características agrícolas
BR112014009771A2 (pt) método para controlar a vegetação, métodos para a identifacação de um herbicida e de uma sequência de nucleotídeos, ácido nucleico, célula vegetal, plantas, semente, métodos de produção de uma célula vegetal e de uma planta e método de identificação
DK3460062T3 (da) Isolerede polynukleotider og polypeptider og fremgangsmåde til anvendelse deraf til øgning af planters udbytte og/eller landbrugsmæssige egenskaber
HK1184492A1 (zh) 體細胞至誘導的重編程神經幹細胞 的轉化
BR112014010451A2 (pt) métodos para controlar a vegetação, para a identificação de um herbicida e de identificação de uma sequência de nucleotídeos, ácido nucleico isolado, célula vegetal transgênica, planta transgênica, planta, semente, métodos de produção de uma célula vegetal transgênica e de identificação ou seleção de uma célula vegetal transformada
AR094432A1 (es) Plantas resistentes a hongos que expresan hidrofobina
IL231871A0 (en) Benthamiana nicotiana plants lacking fucosyltransferase activity
BR112014027468A2 (pt) polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.
BR112015008618A2 (pt) métodos de análise de um embrião isolado de uma planta monocotiledônea e de uma planta dicotiledônea
BR112014010537A2 (pt) método para modular a sensibilidade ao etileno, planta transgênica, proteína isolada, sequência de polinucleotídeos isolada, polipeptídeo com atividade regulatória de etileno, método para aumentar o rendimento em uma planta, método para melhorar um parâmetro agronômico de uma planta, método de seleção assistida por marcador de uma planta
DK2811819T4 (da) Fremgangsmåde til samtidig dyrkning af afgrødeplanter og udnyttelse af energien fra sollys
ZA201307514B (en) Method for improving plant resistance to insects
UA109110C2 (ru) Полинуклеотид, который обеспечивает возможность специфической экспрессии в целых семенах однодольного растения
CL2014001570A1 (es) Metodo para analizar un gen ahasl de planta; kit para analizar gen ahasl de planta; cebador directo ahasl
GB201116129D0 (en) Methods and means to produce abiotic stress tolerant plants
DK3064575T3 (da) Fremgangsmåde til differentiering af pluripotent stamcelle induceret fra mesenkymal stamcelle til hepatocyt
HUE039314T2 (hu) Eljárás herbicid-rezisztens cukorrépa növények kifejlesztésére

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: suspension of the patent application procedure
B06A Notification to applicant to reply to the report for non-patentability or inadequacy of the application according art. 36 industrial patent law
B09A Decision: intention to grant
B11D Dismissal acc. art. 38, par 2 of ipl - failure to pay fee after grant in time