BR112013024220B8 - Métodos de introdução de um ácido nucleico de interesse em uma célula vegetal com parede celular, de expressão estável de um gene e de transferência de um gene para uma célula vegetal - Google Patents

Métodos de introdução de um ácido nucleico de interesse em uma célula vegetal com parede celular, de expressão estável de um gene e de transferência de um gene para uma célula vegetal

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Publication number
BR112013024220B8
BR112013024220B8 BR112013024220A BR112013024220A BR112013024220B8 BR 112013024220 B8 BR112013024220 B8 BR 112013024220B8 BR 112013024220 A BR112013024220 A BR 112013024220A BR 112013024220 A BR112013024220 A BR 112013024220A BR 112013024220 B8 BR112013024220 B8 BR 112013024220B8
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BR
Brazil
Prior art keywords
cell
gene
plant cell
methods
interest
Prior art date
Application number
BR112013024220A
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English (en)
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BR112013024220A2 (pt
BR112013024220B1 (pt
Inventor
Pon Samuel Jayakumar
Chary Samboju Narasimha
Y Yau Kerrm
Lin Gaofeng
R Webb Steven
G Burroughs Frank
Original Assignee
Dow Agrosciences Llc
Corteva Agriscience Llc
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Publication date
Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BR112013024220A2 publication Critical patent/BR112013024220A2/pt
Publication of BR112013024220B1 publication Critical patent/BR112013024220B1/pt
Publication of BR112013024220B8 publication Critical patent/BR112013024220B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Nanotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medical Informatics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

CONJUGADOS DE PONTO QUÂNTICO E PEPTÍDIO VEÍCULO ADEQUADOS PARA APLICAÇÕES DE FORMAÇÃO DE IMAGENS E DISTRIBUIÇÃO EM PLANTAS. Apresentam-se métodos para a introdução de uma molécula de interesse em uma célula vegetal com uma parede celular mediante uso de um conjugado QD-peptídio com um ponto quântico (QD) com um ou mais peptídios de penetração celular (CPPs). Apresentam-se métodos para a modificação genética ou outra de plantas e para o tratamento ou prevenção de doenças em células vegetais compreendendo uma parede celular.
BR112013024220A 2011-03-23 2012-03-22 Métodos de introdução de um ácido nucleico de interesse em uma célula vegetal com parede celular, de expressão estável de um gene e de transferência de um gene para uma célula vegetal BR112013024220B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161466804P 2011-03-23 2011-03-23
US61/466,804 2011-03-23
US2012129443 2012-09-27

Publications (3)

Publication Number Publication Date
BR112013024220A2 BR112013024220A2 (pt) 2016-12-20
BR112013024220B1 BR112013024220B1 (pt) 2020-12-15
BR112013024220B8 true BR112013024220B8 (pt) 2022-06-28

Family

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Family Applications (1)

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BR112013024220A BR112013024220B8 (pt) 2011-03-23 2012-03-22 Métodos de introdução de um ácido nucleico de interesse em uma célula vegetal com parede celular, de expressão estável de um gene e de transferência de um gene para uma célula vegetal

Country Status (12)

Country Link
US (1) US8609420B2 (pt)
EP (1) EP2697379B1 (pt)
JP (1) JP6067671B2 (pt)
KR (1) KR20140020965A (pt)
CN (1) CN103562397A (pt)
AR (1) AR085555A1 (pt)
AU (1) AU2012230804B2 (pt)
BR (1) BR112013024220B8 (pt)
CA (1) CA2830531C (pt)
IL (1) IL228526B (pt)
RU (1) RU2609637C2 (pt)
WO (1) WO2012129443A2 (pt)

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Also Published As

Publication number Publication date
IL228526B (en) 2018-05-31
CA2830531C (en) 2019-10-15
KR20140020965A (ko) 2014-02-19
US8609420B2 (en) 2013-12-17
CN103562397A (zh) 2014-02-05
CA2830531A1 (en) 2012-09-27
IL228526A0 (en) 2013-12-31
AU2012230804A1 (en) 2013-10-17
EP2697379B1 (en) 2018-08-29
RU2609637C2 (ru) 2017-02-02
WO2012129443A3 (en) 2012-10-26
AU2012230804B2 (en) 2016-12-22
EP2697379A4 (en) 2014-09-03
AR085555A1 (es) 2013-10-09
JP6067671B2 (ja) 2017-01-25
RU2013147198A (ru) 2015-04-27
BR112013024220A2 (pt) 2016-12-20
US20120244569A1 (en) 2012-09-27
EP2697379A2 (en) 2014-02-19
BR112013024220B1 (pt) 2020-12-15
WO2012129443A2 (en) 2012-09-27
NZ616041A (en) 2015-04-24
JP2014511678A (ja) 2014-05-19

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Owner name: CORTEVA AGRISCIENCE LLC (US)