BR112013000434A2 - entrega de molécula de dna linear usando pontos quânticos pegilados para transformação estável em plantas. - Google Patents

entrega de molécula de dna linear usando pontos quânticos pegilados para transformação estável em plantas.

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Publication number
BR112013000434A2
BR112013000434A2 BR112013000434A BR112013000434A BR112013000434A2 BR 112013000434 A2 BR112013000434 A2 BR 112013000434A2 BR 112013000434 A BR112013000434 A BR 112013000434A BR 112013000434 A BR112013000434 A BR 112013000434A BR 112013000434 A2 BR112013000434 A2 BR 112013000434A2
Authority
BR
Brazil
Prior art keywords
plants
quantum dots
dna molecule
cell
stable transformation
Prior art date
Application number
BR112013000434A
Other languages
English (en)
Inventor
Frank G Burroughs
Jayakumar Pon Samuel
Kerrm Y Yau
Narasimha Chary Samboju
Steven R Webb
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BR112013000434A2 publication Critical patent/BR112013000434A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Nanotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medical Informatics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

entrega de molécula de dna linear usando pontos quânticos pegilados para transformação estável em plantas. apresente invenção refere-se a métodos para introdução de uma molécula de ácido nucleico linear de interesse em uma célula vegetal compreendendo uma parede celular incluem o uso de nanopartículas revestidas de polietilenoglicol. em algumas modalidades, a célula compreendendo uma parede celular é uma célula vegetal. os métodos incluem modificar geneticamente ou de outra maneira plantas e para tratamento ou prevenção de doença em células vegetais compreendendo uma parede celular. plantas transgênicas incluem uma molécula de ácido nucleico de interesse produzida pela regeneração de plantas inteiras de células vegetais transformadas com moléculas de ácidos nucleicos lineares.
BR112013000434A 2010-07-07 2011-07-07 entrega de molécula de dna linear usando pontos quânticos pegilados para transformação estável em plantas. BR112013000434A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36222410P 2010-07-07 2010-07-07
PCT/US2011/043221 WO2012006443A2 (en) 2010-07-07 2011-07-07 Linear dna molecule delivery using pegylated quantum dots for stable transformation in plants

Publications (1)

Publication Number Publication Date
BR112013000434A2 true BR112013000434A2 (pt) 2016-05-17

Family

ID=45441804

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013000434A BR112013000434A2 (pt) 2010-07-07 2011-07-07 entrega de molécula de dna linear usando pontos quânticos pegilados para transformação estável em plantas.

Country Status (12)

Country Link
US (1) US8653327B2 (pt)
EP (1) EP2591116B1 (pt)
JP (4) JP2013529934A (pt)
KR (1) KR101840068B1 (pt)
CN (2) CN107267557A (pt)
AU (1) AU2011274811C1 (pt)
BR (1) BR112013000434A2 (pt)
CA (2) CA2804168C (pt)
IL (1) IL223979A (pt)
UA (1) UA113046C2 (pt)
WO (1) WO2012006443A2 (pt)
ZA (1) ZA201300139B (pt)

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WO2016040030A1 (en) 2014-09-12 2016-03-17 E. I. Du Pont De Nemours And Company Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use
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Also Published As

Publication number Publication date
CN103080323A (zh) 2013-05-01
AU2011274811B2 (en) 2015-06-25
EP2591116A2 (en) 2013-05-15
US20120023619A1 (en) 2012-01-26
ZA201300139B (en) 2014-03-26
CA3065095C (en) 2023-02-14
JP2013529934A (ja) 2013-07-25
KR101840068B1 (ko) 2018-03-19
JP2019176872A (ja) 2019-10-17
JP2021180667A (ja) 2021-11-25
US8653327B2 (en) 2014-02-18
RU2013104998A (ru) 2014-08-20
KR20130089643A (ko) 2013-08-12
IL223979A (en) 2017-05-29
AU2011274811A1 (en) 2013-01-24
CA2804168C (en) 2020-02-18
CA3065095A1 (en) 2012-01-12
EP2591116B1 (en) 2019-03-13
WO2012006443A2 (en) 2012-01-12
EP2591116A4 (en) 2014-01-15
CN107267557A (zh) 2017-10-20
CA2804168A1 (en) 2012-01-12
UA113046C2 (xx) 2016-12-12
WO2012006443A3 (en) 2012-03-29
JP2017148047A (ja) 2017-08-31
AU2011274811C1 (en) 2015-09-24

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B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
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