MX2016002266A - Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso. - Google Patents
Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso.Info
- Publication number
- MX2016002266A MX2016002266A MX2016002266A MX2016002266A MX2016002266A MX 2016002266 A MX2016002266 A MX 2016002266A MX 2016002266 A MX2016002266 A MX 2016002266A MX 2016002266 A MX2016002266 A MX 2016002266A MX 2016002266 A MX2016002266 A MX 2016002266A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- genome
- cas endonuclease
- cell
- guide polynucleotide
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 102000004533 Endonucleases Human genes 0.000 title abstract 3
- 108010042407 Endonucleases Proteins 0.000 title abstract 3
- 239000002157 polynucleotide Substances 0.000 title abstract 3
- 102000040430 polynucleotide Human genes 0.000 title abstract 3
- 108091033319 polynucleotide Proteins 0.000 title abstract 3
- 238000012986 modification Methods 0.000 title abstract 2
- 230000004048 modification Effects 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 abstract 3
- 238000009395 breeding Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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Abstract
Se proporciona composiciones y métodos para la modificación genómica de una secuencia objetivo en el genoma de una célula. Los métodos y composiciones usan un sistema de polinucleótido guía/endonucleasa Cas para proporcionar un sistema eficaz para modificar o alterar sitios objetivo dentro del genoma de una célula u organismo. Una vez que se identificó un sitio genómico objetivo puede usarse diversos métodos para modificar, además, los sitios objetivo de manera que contengan diversos polinucleótidos de interés. Además, se proporciona composiciones y métodos para editar una secuencia de nucleótidos en el genoma de una célula. Además, se describen métodos de cultivo y métodos para seleccionar plantas con el uso de un sistema bicomponente de polinucleótido de ARN y endonucleasa CAS.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361868706P | 2013-08-22 | 2013-08-22 | |
US201361882532P | 2013-09-25 | 2013-09-25 | |
US201461937045P | 2014-02-07 | 2014-02-07 | |
US201461953090P | 2014-03-14 | 2014-03-14 | |
US201462023239P | 2014-07-11 | 2014-07-11 | |
PCT/US2014/051780 WO2015026885A1 (en) | 2013-08-22 | 2014-08-20 | Genome modification using guide polynucleotide/cas endonuclease systems and methods of use |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2016002266A true MX2016002266A (es) | 2016-07-26 |
MX361154B MX361154B (es) | 2018-11-28 |
Family
ID=51493053
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2016002306A MX2016002306A (es) | 2013-08-22 | 2014-08-20 | Promotor u6 de polimerasa iii de soja y metodos de uso. |
MX2016002118A MX2016002118A (es) | 2013-08-22 | 2014-08-20 | Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso. |
MX2016002266A MX361154B (es) | 2013-08-22 | 2014-08-20 | Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso. |
MX2016002119A MX363842B (es) | 2013-08-22 | 2014-08-20 | Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas. |
MX2018014501A MX2018014501A (es) | 2013-08-22 | 2016-02-19 | Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso. |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2016002306A MX2016002306A (es) | 2013-08-22 | 2014-08-20 | Promotor u6 de polimerasa iii de soja y metodos de uso. |
MX2016002118A MX2016002118A (es) | 2013-08-22 | 2014-08-20 | Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso. |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
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MX2016002119A MX363842B (es) | 2013-08-22 | 2014-08-20 | Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas. |
MX2018014501A MX2018014501A (es) | 2013-08-22 | 2016-02-19 | Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso. |
Country Status (12)
Country | Link |
---|---|
US (16) | US20150082478A1 (es) |
EP (6) | EP3036333B1 (es) |
CN (4) | CN105916989A (es) |
AU (6) | AU2014308899B2 (es) |
BR (4) | BR112016003578A2 (es) |
CA (7) | CA2922040C (es) |
DK (1) | DK3036327T3 (es) |
ES (1) | ES2729635T3 (es) |
HU (1) | HUE044916T2 (es) |
LT (1) | LT3036327T (es) |
MX (5) | MX2016002306A (es) |
WO (4) | WO2015026883A1 (es) |
Families Citing this family (280)
Publication number | Priority date | Publication date | Assignee | Title |
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MX2013010911A (es) | 2011-03-23 | 2015-03-03 | Pioneer Hi Bred Int | Metodos para producir un locus de rasgo transgenico complejo. |
US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
EP2861737B1 (en) | 2012-06-19 | 2019-04-17 | Regents Of The University Of Minnesota | Gene targeting in plants using dna viruses |
ES2926021T3 (es) * | 2012-10-23 | 2022-10-21 | Toolgen Inc | Composición para escindir un ADN objetivo que comprende un ARN guía específico para el ADN objetivo y ácido nucleico codificador de proteína Cas o proteína Cas, y uso de la misma |
DK2986729T3 (en) | 2013-04-16 | 2018-10-29 | Regeneron Pharma | TARGETED MODIFICATION OF ROOT THROUGH |
WO2014199358A1 (en) | 2013-06-14 | 2014-12-18 | Cellectis | Methods for non-transgenic genome editing in plants |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
AU2014308899B2 (en) | 2013-08-22 | 2020-11-19 | E. I. Du Pont De Nemours And Company | Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker, and compositions thereof |
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