MX2016002306A - Promotor u6 de polimerasa iii de soja y metodos de uso. - Google Patents

Promotor u6 de polimerasa iii de soja y metodos de uso.

Info

Publication number
MX2016002306A
MX2016002306A MX2016002306A MX2016002306A MX2016002306A MX 2016002306 A MX2016002306 A MX 2016002306A MX 2016002306 A MX2016002306 A MX 2016002306A MX 2016002306 A MX2016002306 A MX 2016002306A MX 2016002306 A MX2016002306 A MX 2016002306A
Authority
MX
Mexico
Prior art keywords
soybean
methods
polymerase iii
promoter
plants
Prior art date
Application number
MX2016002306A
Other languages
English (en)
Inventor
Zhongsen Li
Andrew Mark Cigan
Joshua K Young
Saverio Carl Falco
Zhan-Bin Liu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51493053&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2016002306(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Du Pont filed Critical Du Pont
Publication of MX2016002306A publication Critical patent/MX2016002306A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physiology (AREA)
  • Immunology (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)

Abstract

La invención se refiere a secuencias reguladoras de la expresión génica de soja, específicamente al promotor de un gen de U6 polimerasa III de soja y fragmentos de este, y su uso para promover la expresión de uno o más fragmentos de ácidos nucleicos heterólogos en plantas. La invención describe, además, composiciones, constructos de polinucleótidos, células hospederas transformadas, plantas mutadas, plantas y semillas transgénicas que contienen el constructo recombinante con el promotor, y métodos para preparar y usarlas.
MX2016002306A 2013-08-22 2014-08-20 Promotor u6 de polimerasa iii de soja y metodos de uso. MX2016002306A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201361868706P 2013-08-22 2013-08-22
US201361882532P 2013-09-25 2013-09-25
US201461937045P 2014-02-07 2014-02-07
US201461953090P 2014-03-14 2014-03-14
US201462023239P 2014-07-11 2014-07-11
PCT/US2014/051782 WO2015026887A1 (en) 2013-08-22 2014-08-20 A soybean u6 polymerase iii promoter and methods of use

Publications (1)

Publication Number Publication Date
MX2016002306A true MX2016002306A (es) 2016-07-08

Family

ID=51493053

Family Applications (5)

Application Number Title Priority Date Filing Date
MX2016002306A MX2016002306A (es) 2013-08-22 2014-08-20 Promotor u6 de polimerasa iii de soja y metodos de uso.
MX2016002118A MX2016002118A (es) 2013-08-22 2014-08-20 Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.
MX2016002119A MX363842B (es) 2013-08-22 2014-08-20 Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas.
MX2016002266A MX361154B (es) 2013-08-22 2014-08-20 Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso.
MX2018014501A MX2018014501A (es) 2013-08-22 2016-02-19 Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso.

Family Applications After (4)

Application Number Title Priority Date Filing Date
MX2016002118A MX2016002118A (es) 2013-08-22 2014-08-20 Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.
MX2016002119A MX363842B (es) 2013-08-22 2014-08-20 Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas.
MX2016002266A MX361154B (es) 2013-08-22 2014-08-20 Modificación del genoma usando sistemas guía de polinucleótido/cas endonucleasa y métodos de uso.
MX2018014501A MX2018014501A (es) 2013-08-22 2016-02-19 Modificacion del genoma usando sistemas guia de polinucleotido/cas endonucleasa y metodos de uso.

Country Status (12)

Country Link
US (16) US20150059010A1 (es)
EP (6) EP3036332B1 (es)
CN (4) CN105829536A (es)
AU (6) AU2014308900A1 (es)
BR (3) BR112016003591A8 (es)
CA (7) CA2922089C (es)
DK (1) DK3036327T3 (es)
ES (1) ES2729635T3 (es)
HU (1) HUE044916T2 (es)
LT (1) LT3036327T (es)
MX (5) MX2016002306A (es)
WO (4) WO2015026883A1 (es)

Families Citing this family (294)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201391373A1 (ru) 2011-03-23 2014-07-30 Пайонир Хай-Бред Интернэшнл, Инк. Способы получения сложного локуса трансгенных признаков
CA2853829C (en) 2011-07-22 2023-09-26 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
BR112014031891A2 (pt) 2012-06-19 2017-08-01 Univ Minnesota direcionamento genético nas plantas utilizando vírus de dna
SG11201503059XA (en) * 2012-10-23 2015-06-29 Toolgen Inc Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
RS62263B1 (sr) 2013-04-16 2021-09-30 Regeneron Pharma Ciljana modifikacija genoma pacova
US20160145631A1 (en) 2013-06-14 2016-05-26 Cellectis Methods for non-transgenic genome editing in plants
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
MX2016002306A (es) 2013-08-22 2016-07-08 Du Pont Promotor u6 de polimerasa iii de soja y metodos de uso.
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US10584358B2 (en) 2013-10-30 2020-03-10 North Carolina State University Compositions and methods related to a type-II CRISPR-Cas system in Lactobacillus buchneri
CA2930015A1 (en) 2013-11-07 2015-05-14 Editas Medicine, Inc. Crispr-related methods and compositions with governing grnas
RU2725520C2 (ru) 2013-12-11 2020-07-02 Регенерон Фармасьютикалс, Инк. Способы и композиции для направленной модификации генома
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
US10787654B2 (en) 2014-01-24 2020-09-29 North Carolina State University Methods and compositions for sequence guiding Cas9 targeting
US11186843B2 (en) * 2014-02-27 2021-11-30 Monsanto Technology Llc Compositions and methods for site directed genomic modification
CA2944978C (en) 2014-04-08 2024-02-13 North Carolina State University Methods and compositions for rna-directed repression of transcription using crispr-associated genes
US11584936B2 (en) * 2014-06-12 2023-02-21 King Abdullah University Of Science And Technology Targeted viral-mediated plant genome editing using CRISPR /Cas9
AU2015288157A1 (en) 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
US10450584B2 (en) 2014-08-28 2019-10-22 North Carolina State University Cas9 proteins and guiding features for DNA targeting and genome editing
MX2017002930A (es) 2014-09-12 2017-06-06 Du Pont Generacion de sitios de integracion especifica de sitio para loci de rasgos complejos en maiz y soja, y metodos de uso.
US10208298B2 (en) 2014-11-06 2019-02-19 E.I. Du Pont De Nemours And Company Peptide-mediated delivery of RNA-guided endonuclease into cells
SI3221457T1 (sl) * 2014-11-21 2019-08-30 Regeneron Pharmaceuticals, Inc. Postopki in sestavki za ciljno genetsko modifikacijo z uporabo vodilnih RNK v parih
AU2015355546B2 (en) * 2014-12-03 2021-10-14 Agilent Technologies, Inc. Guide RNA with chemical modifications
EP3229586A4 (en) 2014-12-10 2018-10-24 Regents of the University of Minnesota Genetically modified cells, tissues, and organs for treating disease
WO2016100309A1 (en) 2014-12-16 2016-06-23 Pioneer Hi-Bred International, Inc. Restoration of male fertility in wheat
DK3234133T3 (da) 2014-12-18 2021-02-08 Integrated Dna Tech Inc Crispr-baserede sammensætninger og fremgangsmåder til anvendelse
US20180179523A1 (en) * 2014-12-18 2018-06-28 Integrated Dna Technologies, Inc. Crispr-based compositions and methods of use
WO2016119703A1 (zh) 2015-01-27 2016-08-04 中国科学院遗传与发育生物学研究所 一种通过基因瞬时表达对整株植物定点改造的方法
CN111518811A (zh) * 2015-01-28 2020-08-11 先锋国际良种公司 Crispr杂合dna/rna多核苷酸及使用方法
EP3262062A1 (en) 2015-02-04 2018-01-03 Pioneer Hi-Bred International, Inc. Novel bt toxin receptors and methods of use
EP3262171A2 (en) 2015-02-23 2018-01-03 Crispr Therapeutics AG Materials and methods for treatment of hemoglobinopathies
AU2016223151A1 (en) * 2015-02-25 2017-08-03 Pioneer Hi-Bred International, Inc. Composition and methods for regulated expression of a guide RNA/Cas endonuclease complex
AU2016239037B2 (en) 2015-03-16 2022-04-21 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Method of applying non-genetic substance to perform site-directed reform of plant genome
BR112017017279A2 (pt) * 2015-03-19 2018-04-17 Pioneer Hi Bred Int métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta
BR112017017260A2 (pt) 2015-03-27 2018-04-17 Du Pont construções de dna, vetor, célula, plantas, semente, método de expressão de rna e método de modificação de local alvo
EP3280803B1 (en) 2015-04-06 2021-05-26 The Board of Trustees of the Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
MX2017014561A (es) 2015-05-15 2018-03-02 Pioneer Hi Bred Int Nuevos sistemas de arn guia/endonucleasa cas.
EP3095870A1 (en) * 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
DE102015006335A1 (de) 2015-05-19 2016-11-24 Kws Saat Se Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
DE102015014252A1 (de) 2015-11-05 2017-05-11 Kws Saat Se Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
EP4039816A1 (en) 2015-05-29 2022-08-10 North Carolina State University Methods for screening bacteria, archaea, algae, and yeast using crispr nucleic acids
WO2016196887A1 (en) 2015-06-03 2016-12-08 Board Of Regents Of The University Of Nebraska Dna editing using single-stranded dna
JP7051438B2 (ja) 2015-06-15 2022-04-11 ノース カロライナ ステート ユニバーシティ 核酸およびｒｎａに基づく抗菌剤の効率的な送達のための方法および組成物
US20180305707A1 (en) * 2015-06-26 2018-10-25 Pioneer Hi-Bred International, Inc. Gos2 regulatory elements and use thereof
CA2990699A1 (en) * 2015-06-29 2017-01-05 Ionis Pharmaceuticals, Inc. Modified crispr rna and modified single crispr rna and uses thereof
WO2017004279A2 (en) * 2015-06-29 2017-01-05 Massachusetts Institute Of Technology Compositions comprising nucleic acids and methods of using the same
EP3328399B1 (en) 2015-07-31 2023-12-27 Regents of the University of Minnesota Modified cells and methods of therapy
US9580727B1 (en) 2015-08-07 2017-02-28 Caribou Biosciences, Inc. Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides
AU2016309392A1 (en) * 2015-08-14 2018-02-22 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Method for obtaining glyphosate-resistant rice by site-directed nucleotide substitution
US11236347B2 (en) 2015-08-28 2022-02-01 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
IL241462A0 (en) 2015-09-10 2015-11-30 Yeda Res & Dev Heterologous engineering of betalain pigments in plants
WO2017053729A1 (en) 2015-09-25 2017-03-30 The Board Of Trustees Of The Leland Stanford Junior University Nuclease-mediated genome editing of primary cells and enrichment thereof
EP3356533A1 (en) 2015-09-28 2018-08-08 North Carolina State University Methods and compositions for sequence specific antimicrobials
WO2017059045A1 (en) * 2015-09-30 2017-04-06 Pioneer Hi-Bred International, Inc. Plant epsp synthases and methods of use
EP3356537B1 (en) * 2015-10-02 2022-08-17 Monsanto Technology LLC Recombinant maize b chromosome sequence and uses thereof
CN108513579B (zh) * 2015-10-09 2022-10-04 孟山都技术公司 新颖的rna导向性核酸酶及其用途
JP7011590B2 (ja) * 2015-10-12 2022-02-10 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 細胞内での遺伝子組換えおよび相同組換えの増加のための保護ｄｎａ鋳型および使用方法
EA037614B1 (ru) 2015-10-16 2021-04-21 Пайонир Хай-Бред Интернэшнл, Инк. Получение растений маиса с повышенной устойчивостью к северной пятнистости листьев
AU2016341041A1 (en) 2015-10-20 2018-03-15 Pioneer Hi-Bred International, Inc. Methods and compositions for marker-free genome modification
IL294014B2 (en) 2015-10-23 2024-07-01 Harvard College Nucleobase editors and their uses
EP3159407A1 (en) * 2015-10-23 2017-04-26 Silence Therapeutics (London) Ltd Guide rnas, methods and uses
EP3350327B1 (en) 2015-10-23 2018-09-26 Caribou Biosciences, Inc. Engineered crispr class 2 cross-type nucleic-acid targeting nucleic acids
WO2017077394A2 (en) 2015-11-04 2017-05-11 Crispr Therapeutics Ag Materials and methods for treatment of hemoglobinopathies
ES2953925T3 (es) * 2015-11-04 2023-11-17 Fate Therapeutics Inc Ingeniería genómica de células pluripotentes
WO2017079026A1 (en) 2015-11-06 2017-05-11 E. I. Du Pont De Nemours And Company Generation of complex trait loci in soybean and methods of use
CN106811479B (zh) 2015-11-30 2019-10-25 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用
US10995339B2 (en) 2015-11-30 2021-05-04 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
MX2018006814A (es) * 2015-12-02 2019-08-05 Ceres Inc Metodos para la modificacion genetica de plantas.
WO2017100343A1 (en) * 2015-12-07 2017-06-15 Arc Bio, Llc Methods and compositions for the making and using of guide nucleic acids
US20180362961A1 (en) 2015-12-11 2018-12-20 Danisco Us Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
JP6888906B2 (ja) * 2015-12-11 2021-06-18 株式会社豊田中央研究所 生物体のゲノムの改変方法及びその利用
DK3390631T3 (da) 2015-12-18 2020-07-13 Danisco Us Inc Fremgangsmåder og sammensætninger til t-rna-baseret guide-rna-ekspression
WO2017106767A1 (en) * 2015-12-18 2017-06-22 The Scripps Research Institute Production of unnatural nucleotides using a crispr/cas9 system
BR112018012887B1 (pt) 2015-12-22 2024-02-06 Pioneer Hi-Bred International, Inc Cassete de expressão, vetor, métodos de obtenção de célula vegetal e planta transgênica, métodos para expressar um polinucleotídeo
WO2017112620A1 (en) 2015-12-22 2017-06-29 North Carolina State University Methods and compositions for delivery of crispr based antimicrobials
IL260532B2 (en) 2016-01-11 2023-12-01 Univ Leland Stanford Junior Systems containing chaperone proteins and their uses for controlling gene expression
KR20180095719A (ko) 2016-01-11 2018-08-27 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 키메라 단백질 및 면역요법 방법
US20190017067A1 (en) * 2016-01-12 2019-01-17 Regents Of The University Of Minnesota Glyphosate tolerant plants having modified 5-enolpyruvylshikimate-3-phosphate synthase gene regulation
WO2017132239A1 (en) 2016-01-26 2017-08-03 Pioneer Hi-Bred International, Inc. Waxy corn
KR20180116769A (ko) * 2016-02-09 2018-10-25 시버스 유에스 엘엘씨 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물
WO2017155715A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
EP3699280A3 (en) 2016-03-11 2020-11-18 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
WO2017155714A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
CN107312821A (zh) * 2016-04-25 2017-11-03 中国检验检疫科学研究院 用于转基因大米m12品系特异性基因成分精准定量检测的引物探针、试剂盒及方法
MX2018014599A (es) 2016-05-27 2019-02-28 Aadigen Llc Peptidos y nanoparticulas para suministro intracelular de moleculas editoras de genoma.
BR112018074494A2 (pt) * 2016-06-01 2019-03-19 Kws Saat Se & Co Kgaa sequências de ácidos nucleicos híbridas para engenharia genômica
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
CA3022854A1 (en) 2016-06-14 2017-12-21 Pioneer Hi-Bred International, Inc. Use of cpf1 endonuclease for plant genome modifications
CA3018430A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
CA3029271A1 (en) * 2016-06-28 2018-01-04 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
IL308426A (en) 2016-08-03 2024-01-01 Harvard College Adenosine nuclear base editors and their uses
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CN106350536B (zh) * 2016-08-28 2021-02-12 浙江大学 一种植物杂交体系及其应用
IL247752A0 (en) * 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
MX2019002907A (es) 2016-09-14 2019-09-18 Monsanto Technology Llc Métodos y composiciones para edición de genoma mediante inducción haploide.
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
US11242542B2 (en) 2016-10-07 2022-02-08 Integrated Dna Technologies, Inc. S. pyogenes Cas9 mutant genes and polypeptides encoded by same
CN118726313A (zh) 2016-10-07 2024-10-01 综合Dna技术公司 化脓链球菌cas9突变基因和由其编码的多肽
CA3037560A1 (en) 2016-10-13 2018-04-19 Pioneer Hi-Bred International, Inc. Generating northern leaf blight resistant maize
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
GB2604416B (en) 2016-10-18 2023-03-15 Univ Minnesota Tumor infiltating lymphocytes and methods of therapy
WO2018081728A1 (en) * 2016-10-31 2018-05-03 Emendobio Inc. Compositions for genome editing
CA3042259A1 (en) * 2016-11-04 2018-05-11 Flagship Pioneering Innovations V. Inc. Novel plant cells, plants, and seeds
AR110075A1 (es) * 2016-11-14 2019-02-20 Inst Genetics & Developmental Biology Cas Un método para edición basal en plantas
US11136567B2 (en) 2016-11-22 2021-10-05 Integrated Dna Technologies, Inc. CRISPR/CPF1 systems and methods
WO2018102131A1 (en) 2016-11-29 2018-06-07 Pioneer Hi-Bred International, Inc. Transcriptional terminators for gene expression in plants
US9816093B1 (en) 2016-12-06 2017-11-14 Caribou Biosciences, Inc. Engineered nucleic acid-targeting nucleic acids
WO2018103686A1 (zh) * 2016-12-07 2018-06-14 中国科学院上海生命科学研究院 叶绿体基因组编辑方法
BR112019011445A2 (pt) 2016-12-08 2019-10-08 Syngenta Participations Ag métodos para o melhoramento da frequência de transformação
JP2019536464A (ja) * 2016-12-08 2019-12-19 インテリア セラピューティクス，インコーポレイテッド 修飾されたガイドｒｎａ
WO2018111947A1 (en) 2016-12-12 2018-06-21 Integrated Dna Technologies, Inc. Genome editing enhancement
EA202091316A1 (ru) * 2016-12-22 2020-08-18 Тулджин Инкорпорейтид Обогащенный олеиновой кислотой растительный организм, имеющий генетически модифицированный fad2, и способ его получения
CN117737062A (zh) * 2016-12-22 2024-03-22 因特利亚治疗公司 用于治疗α-1抗胰蛋白酶缺乏症的组合物及方法
CA3047163A1 (en) * 2016-12-22 2018-06-28 Monsanto Technology Llc Genome editing-based crop engineering and production of brachytic plants
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
CN106834341B (zh) * 2016-12-30 2020-06-16 中国农业大学 一种基因定点突变载体及其构建方法和应用
CA3049749A1 (en) * 2017-01-11 2018-07-19 Yeda Research And Development Co. Ltd. Targeted recombination between homologous chromosomes and uses thereof
US11624071B2 (en) 2017-01-28 2023-04-11 Inari Agriculture Technology, Inc. Method of creating a plurality of targeted insertions in a plant cell
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
US11307139B2 (en) 2017-03-03 2022-04-19 Pioneer Hi-Bred International, Inc. Non-destructive assay for soybean seeds using near infrared analysis
CN107066944B (zh) * 2017-03-06 2019-11-05 北京数码视讯科技股份有限公司 一种手指指根的定位方法及装置
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
EP3592777A1 (en) 2017-03-10 2020-01-15 President and Fellows of Harvard College Cytosine to guanine base editor
JP7191388B2 (ja) 2017-03-23 2022-12-19 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸によってプログラム可能なｄｎａ結合蛋白質を含む核酸塩基編集因子
EP3601610A1 (en) 2017-03-30 2020-02-05 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
BR112019020311A2 (pt) * 2017-03-30 2020-04-28 Pioneer Hi Bred Int epsp sintases de planta melhoradas e métodos de uso
EP3601579B1 (en) 2017-03-31 2023-10-04 Pioneer Hi-Bred International, Inc. Expression modulating elements and use thereof
EP3615552A1 (en) 2017-04-24 2020-03-04 DuPont Nutrition Biosciences ApS Methods and compositions of anti-crispr proteins for use in plants
US20200407737A1 (en) 2017-05-03 2020-12-31 KWS SAAT SE & Co. KGaA Use of crispr-cas endonucleases for plant genome engineering
CN117051035A (zh) * 2017-05-05 2023-11-14 苏州齐禾生科生物科技有限公司 不使用转基因标记序列分离细胞的方法
CN115927437A (zh) * 2017-05-11 2023-04-07 中国科学院遗传与发育生物学研究所 抗除草剂基因的产生及其用途
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US20200199604A1 (en) * 2017-05-17 2020-06-25 Cold Spring Harbor Laboratory Compositions and methods for generating weak alleles in plants
WO2018220582A1 (en) * 2017-05-31 2018-12-06 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
GB201708665D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing extractability of solids from coffee beans
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
WO2018224861A1 (en) 2017-06-07 2018-12-13 International Rice Research Institute Increasing hybrid seed production through higher outcrossing rate in cytoplasmic male sterile gramineae plants and related materials and methods
WO2018237197A2 (en) * 2017-06-21 2018-12-27 Noble Research Institute, Llc CRISPR MEDIATED SELECTIVE GENE DISRUPTION IN PLANTS
WO2018237372A1 (en) 2017-06-23 2018-12-27 Cornell University RNA MOLECULES, METHODS FOR PRODUCING CIRCULAR RNA, AND METHODS OF TREATMENT
EP3645021A4 (en) 2017-06-30 2021-04-21 Intima Bioscience, Inc. ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
US20210054404A1 (en) * 2017-08-22 2021-02-25 Napigen, Inc. Organelle genome modification using polynucleotide guided endonuclease
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
WO2019055141A1 (en) * 2017-09-14 2019-03-21 Pioneer Hi-Bred International, Inc. COMPOSITIONS AND METHODS FOR MODIFYING PLANT SIZE
US20200270632A1 (en) * 2017-09-15 2020-08-27 The Board Of Trustees Of The Leland Stanford Junior University Multiplex production and barcoding of genetically engineered cells
US11555195B2 (en) * 2017-09-18 2023-01-17 Futuragene Israel Ltd. Tissue-specific expression control of DELLA polypeptides
AU2018336128B2 (en) 2017-09-19 2023-01-05 Tropic Biosciences UK Limited Modifying the specificity of plant non-coding RNA molecules for silencing gene expression
CN111373046A (zh) 2017-09-25 2020-07-03 先锋国际良种公司 组织偏好性启动子和使用方法
US11603536B2 (en) 2017-09-29 2023-03-14 Inari Agriculture Technology, Inc. Methods for efficient maize genome editing
CN111465321B (zh) 2017-10-13 2022-12-06 先锋国际良种公司 用于植物细胞的细胞重编程的系统和方法
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
WO2019084306A1 (en) * 2017-10-26 2019-05-02 University Of Florida Research Foundation, Inc. METHOD FOR REDUCING GENOMIC COMPLEXITY AND DETECTING POLYMORPHISMS
CA3074617A1 (en) 2017-11-16 2019-05-23 Dow Agrosciences Llc Plant promoter for transgene expression
AU2018386002B2 (en) * 2017-12-15 2024-07-25 Danisco Us Inc CAS9 variants and methods of use
US11293019B2 (en) 2017-12-22 2022-04-05 Gflas Life Sciences, Inc. Chimeric genome engineering molecules and methods
US11672827B2 (en) 2017-12-23 2023-06-13 Uwell Biopharma Inc. Pharmaceutical chimeric receptor composition and method thereof
CA3084365A1 (en) 2017-12-27 2019-07-04 Pioneer Hi-Bred International, Inc. Transformation of dicot plants
CN111527638B (zh) * 2017-12-28 2024-09-20 日立造船株式会社 全固态电池的制造方法
CN109971763A (zh) * 2017-12-28 2019-07-05 未名生物农业集团有限公司 花期调控基因cmp1和相关的载体及其应用
US11634722B2 (en) 2018-01-22 2023-04-25 Inari Agriculture Technology, Inc. Plant gene editing systems, methods, and compositions
US20240318171A1 (en) * 2018-01-26 2024-09-26 Integrated Dna Technologies, Inc. Crispr-based compositions and methods of use
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US11802288B1 (en) 2018-01-29 2023-10-31 Inari Agriculture Technology, Inc. Methods for efficient soybean genome editing
US11220694B1 (en) 2018-01-29 2022-01-11 Inari Agriculture, Inc. Rice cells and rice plants
EP3749768A1 (en) 2018-02-05 2020-12-16 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of hemoglobinopathies
US11566236B2 (en) 2018-02-05 2023-01-31 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of hemoglobinopathies
US12060586B2 (en) * 2018-02-15 2024-08-13 The Broad Institute, Inc. Cell data recorders and uses thereof
US20210105962A1 (en) * 2018-02-22 2021-04-15 Elsoms Developments Ltd Methods and compositions relating to maintainer lines
WO2019165168A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019173125A1 (en) 2018-03-09 2019-09-12 Pioneer Hi-Bred International, Inc. Compositions and methods for modification of fatty acids in soybean
CN111868247A (zh) * 2018-03-12 2020-10-30 先锋国际良种公司 用于植物转化的方法
BR112020019198A2 (pt) 2018-03-23 2021-02-17 Pioneer Hi-Bred International, Inc. método para identificar um gene r em uma planta, método para obter uma planta resistente a doenças, planta modificada tendo resistência aumentada a uma doença, método para introduzir uma variante alélica de um gene nlr01, construto de dna recombinante, célula de planta transgênica e planta transgênica
CN110396523B (zh) * 2018-04-23 2023-06-09 中国科学院分子植物科学卓越创新中心 一种重复片段介导的植物定点重组方法
WO2019205919A1 (zh) * 2018-04-28 2019-10-31 青岛清原化合物有限公司 一种生物突变体库的构建方法
CN112088018A (zh) * 2018-05-07 2020-12-15 先锋国际良种公司 用于在植物细胞基因组中同源定向修复双链断裂的方法和组合物
CN112105732A (zh) * 2018-05-10 2020-12-18 先正达参股股份有限公司 用于多核苷酸的靶向编辑的方法和组合物
KR20210045360A (ko) 2018-05-16 2021-04-26 신테고 코포레이션 가이드 rna 설계 및 사용을 위한 방법 및 시스템
CN108642077A (zh) * 2018-05-18 2018-10-12 江苏省农业科学院 基于CRISPR/Cas9基因编辑技术选育绿豆不育突变体的方法及专用gRNA
US20210180079A1 (en) * 2018-05-21 2021-06-17 Pioneer Hi-Bred International, Inc. Modification of glutamine synthetase to improve yield in plants
EP3802887A2 (en) * 2018-05-25 2021-04-14 Pioneer Hi-Bred International, Inc. Systems and methods for improved breeding by modulating recombination rates
EP3575402A1 (en) * 2018-06-01 2019-12-04 Algentech SAS Gene targeting
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
WO2020006112A1 (en) 2018-06-26 2020-01-02 Regents Of The University Of Minnesota Delivery of developmental regulators to plants for the induction of meristematic tissue with genetic alterations
WO2020005933A1 (en) 2018-06-28 2020-01-02 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
US20210324398A1 (en) 2018-06-29 2021-10-21 Pioneer Hi-Bred International, Inc. Edited nac genes in plants
CA3108767A1 (en) * 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US20220380418A1 (en) 2018-08-13 2022-12-01 Pioneer Hi-Bred International, Inc. Novel insecticidal toxin receptors and methods of use
WO2020081149A2 (en) * 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
US11459551B1 (en) 2018-08-31 2022-10-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
CN109136258A (zh) * 2018-09-06 2019-01-04 先正达参股股份有限公司 小麦中基因编辑效率的优化
CN113015801A (zh) * 2018-09-20 2021-06-22 赛诺菲 基于内含子的通用克隆方法和组合物
EP3861120A4 (en) 2018-10-01 2023-08-16 North Carolina State University RECOMBINANT TYPE I CRISPR-CAS SYSTEM
BR112021004235A2 (pt) * 2018-10-02 2021-05-18 Monsanto Technology Llc composições e métodos para transferência de biomoléculas para células feridas
BR112021007306A2 (pt) * 2018-10-16 2021-07-27 Pioneer Hi-Bred International, Inc. mapeamento fino por edição genômica e identificação de gene causal
US11407995B1 (en) 2018-10-26 2022-08-09 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
BR112021008329A2 (pt) 2018-10-31 2021-08-03 Pioneer Hi-Bred International, Inc. composições e métodos para transformação de plantas mediada por ochrobactrum
KR20210084557A (ko) 2018-10-31 2021-07-07 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 오크로박트럼-매개 유전자 편집을 위한 조성물 및 방법
EP3874048A1 (en) * 2018-11-01 2021-09-08 Keygene N.V. Dual guide rna for crispr/cas genome editing in plants cells
US11434477B1 (en) 2018-11-02 2022-09-06 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
US11518999B1 (en) 2018-11-09 2022-12-06 Inari Agriculture Technology, Inc. Plant transformation
CN113473845A (zh) * 2018-12-04 2021-10-01 先正达农作物保护股份公司 经由基因组编辑进行基因沉默
CA3122465A1 (en) * 2018-12-12 2020-06-18 Kyushu University, National University Corporation Production method for genome-edited cells
CN113166744A (zh) 2018-12-14 2021-07-23 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
US11453885B1 (en) 2019-02-19 2022-09-27 Inari Agriculture Technology, Inc. Plant transformation
WO2020185907A1 (en) * 2019-03-11 2020-09-17 Colorado State University Research Foundation Herbicide tolerant plants and production and detection of same
GB201903520D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
GB201903519D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
WO2020191243A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020198408A1 (en) 2019-03-27 2020-10-01 Pioneer Hi-Bred International, Inc. Plant explant transformation
US12031149B2 (en) * 2019-03-27 2024-07-09 Emendobio Inc. Compositions and methods for promoting gene editing of CXCR4 gene
CA3136113A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
JP2022526982A (ja) 2019-04-05 2022-05-27 ダニスコ・ユーエス・インク 線状組換えＤＮＡコンストラクトを使用してバチルス（Ｂａｃｉｌｌｕｓ）のゲノムにドナーＤＮＡ配列を組み込むための方法及びその組成物
JP2022529771A (ja) * 2019-04-24 2022-06-24 オレゴン ヘルス アンド サイエンス ユニヴァーシティ プロモーター配列及び関連製品並びにそれらの使用
US11692197B2 (en) 2019-05-06 2023-07-04 Inari Agriculture Technology, Inc. Delivery of biological molecules to plant cells
CN110241121B (zh) * 2019-05-21 2022-03-29 南京农业大学 大豆E3泛素连接酶GmNLA1编码基因的应用
MX2021015795A (es) * 2019-06-19 2022-01-27 Betterseeds Ltd Un metodo para aumentar el rendimiento de cannabis a traves de la edicion de genes.
JP2022539338A (ja) 2019-06-25 2022-09-08 イナリ アグリカルチャー テクノロジー， インコーポレイテッド 改良された相同性依存性修復ゲノム編集
US20210047651A1 (en) * 2019-07-12 2021-02-18 The Regents Of The University Of California Compositions and methods for targeted insertion of selectable marker-free dna sequence in plants
US11746354B2 (en) 2019-07-19 2023-09-05 Inari Agriculture Technology, Inc. Homology dependent repair genome editing
CN110358767B (zh) * 2019-07-30 2021-06-08 湖北大学 一种基于CRISPR-Cas12a系统的运动发酵单胞菌基因组编辑方法及其应用
CN115244176A (zh) * 2019-08-19 2022-10-25 钟明宏 向导rna-cas蛋白复合物的缀合物
EP3798301A1 (en) * 2019-09-25 2021-03-31 KWS SAAT SE & Co. KGaA Promoter repression
US11926834B2 (en) 2019-11-05 2024-03-12 Pairwise Plants Services, Inc. Compositions and methods for RNA-encoded DNA-replacement of alleles
CN112779266A (zh) * 2019-11-06 2021-05-11 青岛清原化合物有限公司 在生物体内创制新基因的方法及应用
WO2021100034A1 (en) 2019-11-19 2021-05-27 Protalix Ltd. Removal of constructs from transformed cells
CN114746555A (zh) * 2019-11-25 2022-07-12 先正达农作物保护股份公司 大豆属调节元件及其用途
WO2021113264A1 (en) * 2019-12-02 2021-06-10 The Regents Of The University Of California Engineering circular guide rnas
CN111057713A (zh) * 2019-12-18 2020-04-24 广东省微生物研究所（广东省微生物分析检测中心） 一种适用于埃德菌FS110的CRISPR/Cas9载体及其构建方法和应用
US20230059309A1 (en) * 2020-01-09 2023-02-23 Pioneer Hi-Bred International, Inc. Two-step gene swap
US20210222183A1 (en) * 2020-01-22 2021-07-22 Pioneer Hi-Bred International, Inc. Dominant crispr inverted-repeat alleles to down-regulate gene expression in heterozygous plants
BR112022015868A2 (pt) * 2020-02-12 2022-10-04 Pioneer Hi Bred Int Reparo dirigido por homologia mediada por cas em tecido vegetal somático
AU2021226415A1 (en) 2020-02-28 2022-09-15 Pioneer Hi-Bred International, Inc. Sorghum doubled haploid production system
WO2021183874A1 (en) * 2020-03-13 2021-09-16 Pairwise Plants Services, Inc. Methods of introducing variation into plants and products produced therefrom
CN115484815A (zh) * 2020-03-30 2022-12-16 伊纳瑞农业技术有限公司 用于在大豆中表达rna指导的核酸酶和dna结合蛋白的改进多核苷酸
EP4127710A2 (en) 2020-04-02 2023-02-08 Pioneer Hi-Bred International, Inc. Novel insecticidal toxin receptors and methods of use
WO2021211227A1 (en) 2020-04-15 2021-10-21 Pioneer Hi-Bred International, Inc. Plant pathogen effector and disease resistance gene identification, compositions, and methods of use
TW202208626A (zh) * 2020-04-24 2022-03-01 美商生命編輯公司 Rna引導核酸酶及其活性片段與變體，以及使用方法
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
CN115697043A (zh) * 2020-05-13 2023-02-03 纽海姆有限公司 通过靶向诱变获得突变植物的方法
KR20230019149A (ko) * 2020-06-03 2023-02-07 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 옥수수 이벤트 dp-915635-4 및 이의 검출 방법
CN111778274A (zh) * 2020-06-09 2020-10-16 新疆生产建设兵团第六师农业科学研究所 一种通过基因编辑提高番茄耐贮藏性的方法
US20230220409A1 (en) 2020-06-12 2023-07-13 Pioneer Hi-Bred International, Inc. Alteration of seed composition in plants
US11359210B2 (en) 2020-07-31 2022-06-14 Inari Agriculture Technology, Inc. INIR12 transgenic maize
US20240011042A1 (en) 2020-07-31 2024-01-11 Inari Agriculture Technology, Inc. Excisable plant transgenic loci with signature protospacer adjacent motifs or signature guide rna recognition sites
US11242534B1 (en) 2020-07-31 2022-02-08 Inari Agriculture Technology, Inc. INHT31 transgenic soybean
US11369073B2 (en) 2020-07-31 2022-06-28 Inari Agriculture Technology, Inc. INIR12 transgenic maize
US11214811B1 (en) 2020-07-31 2022-01-04 Inari Agriculture Technology, Inc. INIR6 transgenic maize
US11326177B2 (en) 2020-07-31 2022-05-10 Inari Agriculture Technology, Inc. INIR12 transgenic maize
WO2022031720A1 (en) * 2020-08-03 2022-02-10 Arcadia Biosciences, Inc. Dna constructs containing rna polymerase iii promoters from cannabis, and methods of their use
EP4199703A1 (en) 2020-08-18 2023-06-28 International Rice Research Institute Methods of increasing outcrossing rates in gramineae
EP4200425A1 (en) 2020-08-18 2023-06-28 Pioneer Hi-Bred International, Inc. Multiple disease resistance genes and genomic stacks thereof
CN116056564A (zh) * 2020-09-10 2023-05-02 孟山都技术公司 利用减数分裂和种系启动子增加基因编辑和定点整合事件
KR102579767B1 (ko) * 2020-09-21 2023-09-18 서울대학교산학협력단 eIF4E1 유전자 교정에 의해 포티바이러스 저항성이 증가된 유전체 교정 토마토 식물체의 제조방법 및 상기 방법에 의해 제조된 포티바이러스 저항성이 증가된 유전체 교정 토마토 식물체
US11198885B1 (en) 2020-09-28 2021-12-14 Inari Agriculture Technology, Inc. Genetic regulatory element
US11976291B2 (en) 2020-09-28 2024-05-07 Inari Agriculture Technology, Inc. Genetically enhanced maize plants
JP2023545999A (ja) 2020-10-05 2023-11-01 プロタリクス リミテッド ダイサー（ｄｉｃｅｒ）様ノックアウト植物細胞
CN112481259B (zh) * 2020-11-24 2022-09-16 南昌大学 两种甘薯U6基因启动子IbU6的克隆与应用
BR112023011433A2 (pt) * 2020-12-11 2024-02-06 Celanese Int Corp Espuma formada de composição de éster de celulose
JP2024516693A (ja) * 2021-05-02 2024-04-16 チンタオ、キングアグルート、ケミカル、コンパウンド、カンパニー、リミテッド 生物内で新規遺伝子を生成する方法およびその利用
GB202109586D0 (en) 2021-07-02 2021-08-18 Tropic Biosciences Uk Ltd Method for editing banana genes
WO2023275255A1 (en) 2021-07-02 2023-01-05 Tropic Biosciences UK Limited Delay or prevention of browning in banana fruit
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
JP2024534945A (ja) 2021-09-10 2024-09-26 アジレント・テクノロジーズ・インク 化学修飾を有するプライム編集のためのガイドｒｎａ
AU2022375817A1 (en) 2021-11-01 2024-05-09 Flagship Pioneering Innovations Vii, Llc Polynucleotides for modifying organisms
EP4441208A1 (en) 2021-11-30 2024-10-09 Pioneer Hi-Bred International, Inc. High efficiency large scale chromosomal genome manipulation
IL314307A (en) 2022-01-20 2024-09-01 Flagship Pioneering Innovations Vii Llc Polynucleotides for transforming organisms
WO2023172875A1 (en) * 2022-03-07 2023-09-14 Inedita Bio, Inc. Methods and compositions for intron mediated- expression of regulatory elements for trait development
CN114836446B (zh) * 2022-04-26 2023-12-19 上海交通大学 一种抗草甘膦的植物及其制法
EP4299739A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
EP4299733A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005863A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024006802A1 (en) 2022-06-30 2024-01-04 Pioneer Hi-Bred International, Inc. Artificial intelligence-mediated methods and systems for genome editing
WO2024005864A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024020360A1 (en) 2022-07-18 2024-01-25 Pairwise Plants Services, Inc. Mustard green plants named 'pwrg-1', 'pwrg-2,' and 'pwsgc'
WO2024026232A1 (en) * 2022-07-27 2024-02-01 Pioneer Hi-Bred International, Inc Guide rna trapped genome editing
WO2024023578A1 (en) 2022-07-28 2024-02-01 Institut Pasteur Hsc70-4 in host-induced and spray-induced gene silencing
WO2024036190A2 (en) 2022-08-09 2024-02-15 Pioneer Hi-Bred International, Inc. Guide polynucleotide multiplexing
WO2024118876A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a recombinant self-excisable selection marker system
WO2024118881A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative muliplex genome engineering in microbial cells using a bidirectional selection marker system
WO2024118882A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a selection marker swapping system
WO2024123786A1 (en) 2022-12-06 2024-06-13 Pioneer Hi-Bred International, Inc. Methods and compositions for co-delivery of t-dnas expressing multiple guide polynucleotides into plants
WO2024137918A1 (en) 2022-12-21 2024-06-27 Donald Danforth Plant Science Center Antimicrobial peptides and modifications thereof
CN115762641B (zh) * 2023-01-10 2023-04-07 天津极智基因科技有限公司 一种指纹图谱构建方法及系统
WO2024196921A1 (en) 2023-03-20 2024-09-26 Pioneer Hi-Bred International, Inc. Cas polypeptides with altered pam recognition

Family Cites Families (206)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1230061A (en) 1916-02-25 1917-06-12 Joseph A Blanchette Oil-can.
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5196525A (en) 1987-01-13 1993-03-23 University Of British Columbia DNA construct for enhancing the efficiency of transcription
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5959177A (en) * 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
ES2187497T3 (es) 1990-04-12 2003-06-16 Syngenta Participations Ag Promotores preferentemente en tejidos.
ES2171391T3 (es) 1990-04-26 2002-09-16 Aventis Cropscience Nv Nueva cepa de bacillus thuringiensis y su gen de codificado de toxina insecticida.
US5478369A (en) 1990-06-12 1995-12-26 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
DE69227911T2 (de) 1991-08-02 1999-05-12 Kubota Corp., Tokio/Tokyo Neuer mikroorganismus und insektizid
DE69230290T2 (de) 1991-08-27 2000-07-20 Novartis Ag, Basel Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
EP0612208B1 (en) 1991-10-04 2004-09-15 North Carolina State University Pathogen-resistant transgenic plants
TW261517B (es) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
DE69233118T2 (de) 1991-12-04 2004-04-15 E.I. Du Pont De Nemours And Co., Wilmington Fettsäure-desaturase gene aus pflanzen
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5474896A (en) 1992-05-05 1995-12-12 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
AU670316B2 (en) 1992-07-27 1996-07-11 Pioneer Hi-Bred International, Inc. An improved method of (agrobacterium)-mediated transformation of cultured soybean cells
DK0668919T3 (da) 1992-11-17 2003-09-15 Du Pont Gener for mikorsomale delta-12-fedtsyredesaturaser og beslægtede enzymer fra planter
AU5676394A (en) 1992-11-20 1994-06-22 Agracetus, Inc. Transgenic cotton plants producing heterologous bioplastic
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
CA2161881C (en) 1993-01-13 2001-03-27 A. Gururaj Rao High lysine derivatives of alpha-hordothionin
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5837850A (en) 1994-04-21 1998-11-17 Pioneer Hi-Bred International, Inc. Regulatory element conferring tapetum specificity
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US7262055B2 (en) * 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
US5736514A (en) 1994-10-14 1998-04-07 Nissan Chemical Industries, Ltd. Bacillus strain and harmful organism controlling agents
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
EP0828835A1 (en) 1995-06-02 1998-03-18 Pioneer Hi-Bred International, Inc. HIGH THREONINE DERIVATIVES OF $g(a)-HORDOTHIONIN
EP0832235A1 (en) 1995-06-02 1998-04-01 Pioneer Hi-Bred International, Inc. HIGH METHIONINE DERIVATIVES OF alfa-HORDOTHIONIN
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
US5850016A (en) 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
WO1998001575A1 (en) 1996-07-08 1998-01-15 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
EP0946729A2 (en) 1996-11-01 1999-10-06 Pioneer Hi-Bred International, Inc. Proteins with enhanced levels of essential amino acids
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US7053282B1 (en) 1998-02-09 2006-05-30 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
ES2273127T3 (es) 1998-02-26 2007-05-01 Pioneer Hi-Bred International, Inc. Promotor alfa-tubulin 3-18 del maiz.
US6518485B1 (en) * 1998-06-04 2003-02-11 Westvaco Corporation Particle-mediated conifer transformation
ATE309362T1 (de) 1998-08-20 2005-11-15 Pioneer Hi Bred Int Samen-bevorzugende promotoren
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
US6504083B1 (en) 1998-10-06 2003-01-07 Pioneer Hi-Bred International, Inc. Maize Gos-2 promoters
WO2000066748A1 (en) 1999-04-29 2000-11-09 Syngenta Limited Herbicide resistant plants
US20100293669A2 (en) * 1999-05-06 2010-11-18 Jingdong Liu Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement
US7868149B2 (en) * 1999-07-20 2011-01-11 Monsanto Technology Llc Plant genome sequence and uses thereof
US6603061B1 (en) * 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
US20070083945A1 (en) * 2000-03-10 2007-04-12 Byrum Joseph R Nucleic acid molecules and other molecules associated with plants
US6627797B1 (en) * 2000-03-21 2003-09-30 The Texas A&M University System Maize lipoxygenase polynucleotide and methods of use
US6683231B2 (en) 2000-06-02 2004-01-27 E. I. Du Pont De Nemours And Company High level production of p-hydroxybenzoic acid in green plants
EP1320618B1 (en) 2000-09-26 2012-03-07 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US7517975B2 (en) 2000-09-26 2009-04-14 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
US7612251B2 (en) 2000-09-26 2009-11-03 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
AU2001287862B2 (en) 2000-09-29 2006-12-14 Syngenta Limited Herbicide resistant plants
DE10131786A1 (de) 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
US20090100536A1 (en) * 2001-12-04 2009-04-16 Monsanto Company Transgenic plants with enhanced agronomic traits
US20030186281A1 (en) 2001-12-21 2003-10-02 Wolfgang Hillen Modified tetracycline repressor protein compositions and methods of use
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
EP2522723B1 (en) 2003-01-28 2014-12-03 Cellectis Custom-made meganuclease and use thereof
CA2516310C (en) * 2003-02-19 2014-12-09 Commonwealth Scientific And Industrial Research Organisation Efficient gene silencing in plants using short dsrna sequences
IES20030696A2 (en) 2003-09-23 2005-03-23 Carsphairn Ltd A pipeline apparatus
PT2025756E (pt) 2003-11-18 2011-09-28 Bayer Bioscience Nv Inserção direccionada de adn em plantas
WO2005063997A2 (en) 2003-12-22 2005-07-14 Pioneer Hi-Bred International, Inc. Maize metallothionein promoter
CA2554644C (en) 2004-02-02 2015-06-16 Pioneer Hi-Bred International, Inc. Ap2 domain transcription factor odp2 (ovule development protein 2) and methods of use
US7292055B2 (en) 2005-04-21 2007-11-06 Endicott Interconnect Technologies, Inc. Interposer for use with test apparatus
CA2615797C (en) 2005-07-18 2014-04-29 Pioneer Hi-Bred International, Inc. Modified frt recombination sites and methods of use
US20070016985A1 (en) 2005-07-18 2007-01-18 Pioneer Hi-Bred International, Inc. Particle Preparation for Direct-Delivery Transformation
US10066233B2 (en) 2005-08-26 2018-09-04 Dupont Nutrition Biosciences Aps Method of modulating cell resistance
EP3339441A1 (en) 2005-10-13 2018-06-27 Monsanto Technology LLC Methods for producing hybrid seed
US8021867B2 (en) 2005-10-18 2011-09-20 Duke University Rationally-designed meganucleases with altered sequence specificity and DNA-binding affinity
ES2531882T3 (es) 2006-01-12 2015-03-20 Cibus Europe B.V. EPSPS mutantes
KR101447300B1 (ko) 2006-08-11 2014-10-07 몬산토 테크놀로지 엘엘씨 안트라닐레이트 신타제의 엽록체를 표적으로 하는 발현에 의한 고-트립토판 옥수수의 생산
EP3067425A1 (en) 2006-10-31 2016-09-14 E. I. du Pont de Nemours and Company Soybean event dp-305423-1 and constructs for the generation thereof
CA2673663C (en) 2006-12-29 2015-10-06 Dow Agrosciences Llc In vitro methods for the induction and maintenance of plant cell lines as single suspension cells with intact cell walls, and transformation thereof
BRPI0814244B1 (pt) 2007-06-07 2018-10-09 Agriculture And Agri Food Canada método para obtenção de uma planta geneticamente engenheirada
CA2691440A1 (en) 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
US7919676B2 (en) 2007-08-03 2011-04-05 Pioneer Hi-Bred International, Inc. Msca1 nucleotide sequences impacting plant male fertility and method of using same
CN101878307B (zh) 2007-09-27 2017-07-28 陶氏益农公司 以5‑烯醇式丙酮酰莽草酸‑3‑磷酸合酶基因为靶的改造锌指蛋白
US8722410B2 (en) 2007-10-05 2014-05-13 Dow Agrosciences, Llc. Methods for transferring molecular substances into plant cells
US8697857B2 (en) 2007-11-20 2014-04-15 E. I. Du Pont De Nemours And Company Soybean EF1A2 promoter and its use in constitutive expression of transgenic genes in plants
US8546553B2 (en) 2008-07-25 2013-10-01 University Of Georgia Research Foundation, Inc. Prokaryotic RNAi-like system and methods of use
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
MX2011003766A (es) 2008-10-28 2011-04-27 Pioneer Hi Bred Int Proteinas represoras responsivas a sulfonilurea.
DK2370575T3 (en) 2008-12-17 2018-02-05 Dow Agrosciences Llc TARGETED INTEGRATION IN THE ZP15 LOCUS
CN102369287B (zh) 2009-04-07 2014-09-17 陶氏益农公司 纳米颗粒介导的序列特异性核酸酶的投递
US8466341B2 (en) 2009-05-04 2013-06-18 Pioneer Hi-Bred International, Inc. Maize 17KD oleosin seed-preferred regulatory element
US20110167516A1 (en) 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
WO2011082310A2 (en) 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted polynucleotide modification
BR112012024589A2 (pt) 2010-03-31 2017-06-20 Dow Agrosciences Llc peptídeo zeína -gama de planta para liberação de biomoléculas em células de plantas
BR112012028805A2 (pt) 2010-05-10 2019-09-24 The Regents Of The Univ Of California E Nereus Pharmaceuticals Inc composições de endorribonuclease e métodos de uso das mesmas.
CA2798941A1 (en) 2010-06-09 2011-12-15 E. I. Du Pont De Nemours And Company Regulatory sequences for modulating transgene expression in plants
CA2804164C (en) 2010-07-07 2019-10-22 Dow Agrosciences Llc Production of functionalized linear dna cassette and quantum dot/nanoparticle mediated delivery in plants
AU2011274811C1 (en) 2010-07-07 2015-09-24 Corteva Agriscience Llc Linear DNA molecule delivery using pegylated quantum dots for stable transformation in plants
WO2012016052A1 (en) 2010-07-28 2012-02-02 True Fit Corporation Fit recommendation via collaborative inference
WO2012112411A1 (en) 2011-02-15 2012-08-23 Pioneer Hi-Bred International, Inc. Root-preferred promoter and methods of use
EA201391373A1 (ru) 2011-03-23 2014-07-30 Пайонир Хай-Бред Интернэшнл, Инк. Способы получения сложного локуса трансгенных признаков
WO2012129443A2 (en) 2011-03-23 2012-09-27 Dow Agrosciences Llc Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants
US9726249B2 (en) 2011-05-13 2017-08-08 Mississippi State University Shock mitigating materials and methods utilizing spiral shaped elements
US20140113376A1 (en) 2011-06-01 2014-04-24 Rotem Sorek Compositions and methods for downregulating prokaryotic genes
CA2844470A1 (en) 2011-06-21 2013-05-10 Pioneer Hi-Bred International, Inc. Methods and compositions for producing male sterile plants
CA3177512A1 (en) 2011-07-15 2013-01-24 E.I. Du Pont De Nemours And Company Novel plant terminator sequences
EP2740057A4 (en) 2011-08-01 2015-09-09 Tagged Inc GENERAL RECONCILIATION IN A DISTRIBUTED DATABASE
US10538775B2 (en) 2011-08-02 2020-01-21 Pioneer Hi-Bred International, Inc. Terminator sequence for gene expression in plants
EP2739739A1 (en) 2011-08-03 2014-06-11 E. I. Du Pont de Nemours and Company Methods and compositions for targeted integration in a plant
MX348003B (es) 2011-08-22 2017-03-08 Bayer Cropscience Nv Metodos y medios para modificar un genoma vegetal.
US20150159166A1 (en) 2011-10-31 2015-06-11 Pioneer Hi Bred International Inc Plant drought tolerance and nitrogen use efficiency by reducing plant sensitivity to ethylene
WO2013068845A2 (en) 2011-11-07 2013-05-16 The University Of Western Ontario Endonuclease for genome editing
WO2013090734A1 (en) 2011-12-15 2013-06-20 Dow Agrosciences Llc Method for improved transformation using agrobacterium
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
AR089793A1 (es) 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
BR112014028615A2 (pt) 2012-05-18 2017-06-27 Pioneer Hi Bred Int polinucleotídeos, constructo de dna, vetor, célula, plantas, semente, método de expressão, métodos para alterar a expressão e para alterar uma característica de planta.
DK2800811T3 (en) 2012-05-25 2017-07-17 Univ Vienna METHODS AND COMPOSITIONS FOR RNA DIRECTIVE TARGET DNA MODIFICATION AND FOR RNA DIRECTIVE MODULATION OF TRANSCRIPTION
RU2015124938A (ru) 2012-06-04 2017-01-10 Пайонир Хай-Бред Интернэшнл, Инк. Терминаторная последовательность sb-actin для экспрессии генов в растениях
BR112014031891A2 (pt) * 2012-06-19 2017-08-01 Univ Minnesota direcionamento genético nas plantas utilizando vírus de dna
JP2015527889A (ja) 2012-07-25 2015-09-24 ザ ブロード インスティテュート， インコーポレイテッド 誘導可能なｄｎａ結合タンパク質およびゲノム撹乱ツール、ならびにそれらの適用
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
US20140096284A1 (en) 2012-10-01 2014-04-03 Iowa State University Research Foundation, Inc. Method for the delivery of molecules lyophilized onto microparticles to plant tissues
SG11201503059XA (en) 2012-10-23 2015-06-29 Toolgen Inc Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
US20150291967A1 (en) 2012-10-31 2015-10-15 Luc Mathis Coupling herbicide resistance with targeted insertion of transgenes in plants
KR102243092B1 (ko) 2012-12-06 2021-04-22 시그마-알드리치 컴퍼니., 엘엘씨 Crispr-기초된 유전체 변형과 조절
WO2014093479A1 (en) 2012-12-11 2014-06-19 Montana State University Crispr (clustered regularly interspaced short palindromic repeats) rna-guided control of gene regulation
EP3031921A1 (en) 2012-12-12 2016-06-15 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
DK3064585T3 (da) 2012-12-12 2020-04-27 Broad Inst Inc Konstruering og optimering af forbedrede systemer, fremgangsmåder og enzymsammensætninger til sekvensmanipulation
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
EP4286402A3 (en) 2012-12-12 2024-02-14 The Broad Institute, Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
WO2014093655A2 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
SG11201504523UA (en) 2012-12-12 2015-07-30 Broad Inst Inc Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
KR20150105633A (ko) 2012-12-12 2015-09-17 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작을 위한 시스템, 방법 및 최적화된 가이드 조성물의 조작
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
JP2016500268A (ja) 2012-12-13 2016-01-12 ダウ アグロサイエンシィズ エルエルシー トウモロコシにおける特定の遺伝子座に対する精密な遺伝子標的化
CN105121641A (zh) 2012-12-17 2015-12-02 哈佛大学校长及研究员协会 Rna-引导的人类基因组工程化
US10227610B2 (en) 2013-02-25 2019-03-12 Sangamo Therapeutics, Inc. Methods and compositions for enhancing nuclease-mediated gene disruption
CA2905289C (en) 2013-03-12 2023-03-07 Pioneer Hi-Bred International, Inc. Methods for the identification of variant recognition sites for rare-cutting engineered double-strand-break-inducing agents and compositions and uses thereof
EP2971167B1 (en) 2013-03-14 2019-07-31 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
US10113162B2 (en) 2013-03-15 2018-10-30 Cellectis Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes
CA3206344A1 (en) * 2013-03-15 2014-09-18 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
JP2016512048A (ja) 2013-03-15 2016-04-25 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ ＣＲＩＳＰＲ／Ｃａｓシステムを使用した植物ゲノム操作
IL289396B2 (en) 2013-03-15 2023-12-01 The General Hospital Coporation Using tru-grnas to increase the specificity of RNA-guided genome editing
WO2014165825A2 (en) 2013-04-04 2014-10-09 President And Fellows Of Harvard College Therapeutic uses of genome editing with crispr/cas systems
WO2014186686A2 (en) 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
WO2014194190A1 (en) 2013-05-30 2014-12-04 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
CN105517579B (zh) 2013-07-10 2019-11-15 哈佛大学校长及研究员协会 用于RNA向导的基因调节和编辑的正交Cas9蛋白
EP3019619B1 (en) 2013-07-11 2021-08-25 ModernaTX, Inc. Compositions comprising synthetic polynucleotides encoding crispr related proteins and synthetic sgrnas and methods of use
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
WO2015021426A1 (en) 2013-08-09 2015-02-12 Sage Labs, Inc. A crispr/cas system-based novel fusion protein and its application in genome editing
MX2016002306A (es) 2013-08-22 2016-07-08 Du Pont Promotor u6 de polimerasa iii de soja y metodos de uso.
CA2930015A1 (en) 2013-11-07 2015-05-14 Editas Medicine, Inc. Crispr-related methods and compositions with governing grnas
EP3375877A1 (en) 2013-11-18 2018-09-19 Crispr Therapeutics AG Crispr-cas system materials and methods
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
WO2015112896A2 (en) 2014-01-24 2015-07-30 North Carolina State University Methods and compositions for sequences guiding cas9 targeting
US11186843B2 (en) 2014-02-27 2021-11-30 Monsanto Technology Llc Compositions and methods for site directed genomic modification
US11584936B2 (en) 2014-06-12 2023-02-21 King Abdullah University Of Science And Technology Targeted viral-mediated plant genome editing using CRISPR /Cas9
AU2015288157A1 (en) 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
BR112017000621B1 (pt) 2014-07-11 2024-03-12 Pioneer Hi-Bred International, Inc Método para melhorar um traço agronômico de uma planta de milho ou de soja
US10450584B2 (en) 2014-08-28 2019-10-22 North Carolina State University Cas9 proteins and guiding features for DNA targeting and genome editing
MX2017002930A (es) 2014-09-12 2017-06-06 Du Pont Generacion de sitios de integracion especifica de sitio para loci de rasgos complejos en maiz y soja, y metodos de uso.
CN111518811A (zh) 2015-01-28 2020-08-11 先锋国际良种公司 Crispr杂合dna/rna多核苷酸及使用方法
AU2016223151A1 (en) 2015-02-25 2017-08-03 Pioneer Hi-Bred International, Inc. Composition and methods for regulated expression of a guide RNA/Cas endonuclease complex
BR112017017279A2 (pt) 2015-03-19 2018-04-17 Pioneer Hi Bred Int métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta
BR112017017260A2 (pt) 2015-03-27 2018-04-17 Du Pont construções de dna, vetor, célula, plantas, semente, método de expressão de rna e método de modificação de local alvo
MX2017014561A (es) 2015-05-15 2018-03-02 Pioneer Hi Bred Int Nuevos sistemas de arn guia/endonucleasa cas.
DE102015006335A1 (de) 2015-05-19 2016-11-24 Kws Saat Se Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen
AU2016270870A1 (en) 2015-06-02 2018-01-04 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
CA2995843A1 (en) 2015-08-21 2017-03-02 Monsanto Technology Llc Enhanced recombination of genomic loci
CN108513579B (zh) 2015-10-09 2022-10-04 孟山都技术公司 新颖的rna导向性核酸酶及其用途
WO2017066497A2 (en) 2015-10-13 2017-04-20 Duke University Genome engineering with type i crispr systems in eukaryotic cells
AU2016341041A1 (en) 2015-10-20 2018-03-15 Pioneer Hi-Bred International, Inc. Methods and compositions for marker-free genome modification
EP3699280A3 (en) 2016-03-11 2020-11-18 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
WO2017155715A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
WO2017155714A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
CA3022854A1 (en) 2016-06-14 2017-12-21 Pioneer Hi-Bred International, Inc. Use of cpf1 endonuclease for plant genome modifications
CA3018430A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use

Also Published As

Publication number Publication date
AU2014308898B2 (en) 2020-05-14
CA2922040C (en) 2024-02-27
CN105829536A (zh) 2016-08-03
US20230235345A1 (en) 2023-07-27
US11427830B2 (en) 2022-08-30
MX2018014501A (es) 2020-09-14
CA2922090A1 (en) 2015-02-26
US20230279413A1 (en) 2023-09-07
EP3473720A1 (en) 2019-04-24
LT3036327T (lt) 2019-06-25
EP3036333A1 (en) 2016-06-29
CN106133138A (zh) 2016-11-16
EP3036332A1 (en) 2016-06-29
BR112016003561B1 (pt) 2022-10-04
US10519457B2 (en) 2019-12-31
US20160208272A1 (en) 2016-07-21
CA2922090C (en) 2021-05-18
US20160201072A1 (en) 2016-07-14
BR112016003591A8 (pt) 2018-01-30
AU2014308899A1 (en) 2016-03-10
US20150082478A1 (en) 2015-03-19
BR112016003591A2 (pt) 2017-12-05
WO2015026886A1 (en) 2015-02-26
BR112016003776B1 (pt) 2023-12-12
AU2023201788A1 (en) 2023-04-20
EP3036327B1 (en) 2019-05-08
US20160251667A1 (en) 2016-09-01
US20180371479A1 (en) 2018-12-27
AU2014308896A1 (en) 2016-03-10
CN105916987A (zh) 2016-08-31
HUE044916T2 (hu) 2019-11-28
EP3036332B1 (en) 2020-06-24
BR112016003776A2 (pt) 2017-12-05
AU2014308900A1 (en) 2016-03-10
MX363842B (es) 2019-04-05
BR112016003561B8 (pt) 2022-11-01
BR112016003578A2 (pt) 2017-12-05
EP3611268A1 (en) 2020-02-19
CA2922089C (en) 2022-07-05
WO2015026887A1 (en) 2015-02-26
US20200157554A1 (en) 2020-05-21
CA3157565A1 (en) 2015-02-26
WO2015026883A1 (en) 2015-02-26
DK3036327T3 (da) 2019-06-11
WO2015026885A1 (en) 2015-02-26
BR112016003561A2 (pt) 2017-12-05
US20230323374A1 (en) 2023-10-12
US11773400B2 (en) 2023-10-03
US20160208271A1 (en) 2016-07-21
MX2016002118A (es) 2016-06-28
MX2016002119A (es) 2016-06-23
CA3109801A1 (en) 2015-02-26
US20240084318A1 (en) 2024-03-14
CA2922089A1 (en) 2015-02-26
ES2729635T3 (es) 2019-11-05
CA3109801C (en) 2024-01-09
US20200063151A1 (en) 2020-02-27
MX361154B (es) 2018-11-28
US20150059010A1 (en) 2015-02-26
EP3036334A1 (en) 2016-06-29
MX2016002266A (es) 2016-07-26
US10870859B2 (en) 2020-12-22
CA2922040A1 (en) 2015-02-26
EP3036327A1 (en) 2016-06-29
AU2014308899B2 (en) 2020-11-19
CA3221516A1 (en) 2015-02-26
US20220177900A1 (en) 2022-06-09
AU2020264325A1 (en) 2020-11-26
US20190376074A1 (en) 2019-12-12
CN105916989A (zh) 2016-08-31
AU2014308898A1 (en) 2016-03-03
EP3036333B1 (en) 2022-01-19
CA2922046A1 (en) 2015-02-26
US20190040405A1 (en) 2019-02-07

Similar Documents

Publication Publication Date Title
MX2016002306A (es) Promotor u6 de polimerasa iii de soja y metodos de uso.
AU2018204544B2 (en) Plant regulatory elements and uses thereof
MX2019011816A (es) Elementos reguladores de plantas y sus usos.
PH12014502328B1 (en) Plant regulatory elements and uses thereof
MX2020011519A (es) Elementos reguladores vegetales y sus usos.
PH12018502447A1 (en) Plant regulatory elements and uses thereof
PH12017501859A1 (en) Plant promoter for transgene expression
PH12017501858A1 (en) Plant promoter for transgene expression
NZ750837A (en) Plant regulatory elements and uses thereof