MX392008B - Mutaciones de la enzima crispr que reducen los efectos fuera del blanco - Google Patents

Mutaciones de la enzima crispr que reducen los efectos fuera del blanco

Info

Publication number
MX392008B
MX392008B MX2017016289A MX2017016289A MX392008B MX 392008 B MX392008 B MX 392008B MX 2017016289 A MX2017016289 A MX 2017016289A MX 2017016289 A MX2017016289 A MX 2017016289A MX 392008 B MX392008 B MX 392008B
Authority
MX
Mexico
Prior art keywords
crispr
crispr enzyme
target effects
reduce
enzyme
Prior art date
Application number
MX2017016289A
Other languages
English (en)
Other versions
MX2017016289A (es
Inventor
Bernd Zetsche
Feng Zhang
Ian Slaymaker
Linyi Gao
Original Assignee
Broad Inst Inc
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Broad Inst Inc, Massachusetts Inst Technology filed Critical Broad Inst Inc
Publication of MX2017016289A publication Critical patent/MX2017016289A/es
Publication of MX392008B publication Critical patent/MX392008B/es

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    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • G—PHYSICS
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    • G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
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  • Virology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
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  • Cell Biology (AREA)
  • Mathematical Optimization (AREA)
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  • Mathematical Analysis (AREA)
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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

La presente invención se refiere a una proteína CRISPR modificada, caracterizada porque tiene una especificidad aumentada loci polinucleotídicos blanco en comparación con los loci polinucleotídicos fuera del blanco, dicha proteína comprende dos o más modificaciones en el surco de unión entre los dominos RuvC y HNH con referencia a Cas9, en donde las modificaciones comprenden sustituir al menos dos residuos que están cargados positivamente con residuos de aminoácidos que no están cargados o que están cargados negativamente.
MX2017016289A 2015-06-18 2016-06-17 Mutaciones de la enzima crispr que reducen los efectos fuera del blanco MX392008B (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201562181453P 2015-06-18 2015-06-18
US201562207312P 2015-08-19 2015-08-19
US201562237360P 2015-10-05 2015-10-05
US201562255256P 2015-11-13 2015-11-13
US201562269876P 2015-12-18 2015-12-18
PCT/US2016/038034 WO2016205613A1 (en) 2015-06-18 2016-06-17 Crispr enzyme mutations reducing off-target effects

Publications (2)

Publication Number Publication Date
MX2017016289A MX2017016289A (es) 2018-08-15
MX392008B true MX392008B (es) 2025-03-21

Family

ID=56345216

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2017016289A MX392008B (es) 2015-06-18 2016-06-17 Mutaciones de la enzima crispr que reducen los efectos fuera del blanco
MX2022005304A MX2022005304A (es) 2015-06-18 2017-12-14 Mutaciones de la enzima crispr que reducen los efectos fuera del blanco.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2022005304A MX2022005304A (es) 2015-06-18 2017-12-14 Mutaciones de la enzima crispr que reducen los efectos fuera del blanco.

Country Status (14)

Country Link
US (4) US10876100B2 (es)
EP (2) EP3129393B1 (es)
JP (3) JP7107683B2 (es)
KR (2) KR102840885B1 (es)
CN (2) CN108290933A (es)
AU (2) AU2016280893B2 (es)
CA (1) CA2989830A1 (es)
IL (3) IL293323B2 (es)
MX (2) MX392008B (es)
RU (1) RU2752834C2 (es)
SG (1) SG10201912329YA (es)
TW (1) TWI813532B (es)
WO (1) WO2016205613A1 (es)
ZA (1) ZA201708498B (es)

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