BR112022012350A2 - Nucleases de spcas9 de alta fidelidade para modificação de genoma - Google Patents
Nucleases de spcas9 de alta fidelidade para modificação de genomaInfo
- Publication number
- BR112022012350A2 BR112022012350A2 BR112022012350A BR112022012350A BR112022012350A2 BR 112022012350 A2 BR112022012350 A2 BR 112022012350A2 BR 112022012350 A BR112022012350 A BR 112022012350A BR 112022012350 A BR112022012350 A BR 112022012350A BR 112022012350 A2 BR112022012350 A2 BR 112022012350A2
- Authority
- BR
- Brazil
- Prior art keywords
- genome modification
- variants
- fidelity
- spcas9 nucleases
- nucleases
- Prior art date
Links
- 238000012986 modification Methods 0.000 title abstract 3
- 230000004048 modification Effects 0.000 title abstract 3
- 101000910035 Streptococcus pyogenes serotype M1 CRISPR-associated endonuclease Cas9/Csn1 Proteins 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108091033409 CRISPR Proteins 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
NUCLEASES DE SPCAS9 DE ALTA FIDELIDADE PARA MODIFICAÇÃO DE GENOMA. A presente invenção refere-se a variantes e sistemas de proteína Cas9 manipulada, ácidos nucleicos que codificam as variantes e os sistemas de proteínas referidos, e métodos de produção e uso das variantes e dos sistemas de proteínas referidos para modificação de genoma.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202062988279P | 2020-03-11 | 2020-03-11 | |
PCT/US2021/021922 WO2021183771A1 (en) | 2020-03-11 | 2021-03-11 | High fidelity spcas9 nucleases for genome modification |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112022012350A2 true BR112022012350A2 (pt) | 2022-09-27 |
Family
ID=75539896
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022012350A BR112022012350A2 (pt) | 2020-03-11 | 2021-03-11 | Nucleases de spcas9 de alta fidelidade para modificação de genoma |
Country Status (10)
Country | Link |
---|---|
US (1) | US20230058352A1 (pt) |
EP (1) | EP4118197A1 (pt) |
JP (1) | JP2023514327A (pt) |
KR (1) | KR20220128644A (pt) |
CN (1) | CN115244177A (pt) |
AU (1) | AU2021236230A1 (pt) |
BR (1) | BR112022012350A2 (pt) |
CA (1) | CA3163463A1 (pt) |
IL (1) | IL294120B2 (pt) |
WO (1) | WO2021183771A1 (pt) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023077149A1 (en) | 2021-11-01 | 2023-05-04 | Sigma-Aldrich Co. Llc | Electroporation enhancers for crispr-cas systems |
CN117866926A (zh) * | 2024-03-07 | 2024-04-12 | 珠海舒桐医疗科技有限公司 | 一种CRISPR-FrCas9蛋白突变体及应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MXPA04005010A (es) | 2001-11-26 | 2005-04-08 | Advanced Cell Tech Inc | METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS. |
CN109536474A (zh) * | 2015-06-18 | 2019-03-29 | 布罗德研究所有限公司 | 降低脱靶效应的crispr酶突变 |
US11242542B2 (en) * | 2016-10-07 | 2022-02-08 | Integrated Dna Technologies, Inc. | S. pyogenes Cas9 mutant genes and polypeptides encoded by same |
-
2021
- 2021-03-11 JP JP2022549484A patent/JP2023514327A/ja active Pending
- 2021-03-11 BR BR112022012350A patent/BR112022012350A2/pt unknown
- 2021-03-11 CA CA3163463A patent/CA3163463A1/en active Pending
- 2021-03-11 IL IL294120A patent/IL294120B2/en unknown
- 2021-03-11 EP EP21719308.5A patent/EP4118197A1/en active Pending
- 2021-03-11 KR KR1020227027960A patent/KR20220128644A/ko unknown
- 2021-03-11 AU AU2021236230A patent/AU2021236230A1/en active Pending
- 2021-03-11 WO PCT/US2021/021922 patent/WO2021183771A1/en unknown
- 2021-03-11 CN CN202180020084.3A patent/CN115244177A/zh active Pending
- 2021-03-11 US US17/790,392 patent/US20230058352A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA3163463A1 (en) | 2021-09-16 |
IL294120B2 (en) | 2023-10-01 |
AU2021236230A1 (en) | 2022-06-30 |
EP4118197A1 (en) | 2023-01-18 |
IL294120B1 (en) | 2023-06-01 |
US20230058352A1 (en) | 2023-02-23 |
CN115244177A (zh) | 2022-10-25 |
IL294120A (en) | 2022-08-01 |
KR20220128644A (ko) | 2022-09-21 |
JP2023514327A (ja) | 2023-04-05 |
WO2021183771A1 (en) | 2021-09-16 |
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