IN2014DN05937A - - Google Patents

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Publication number
IN2014DN05937A
IN2014DN05937A IN5937DEN2014A IN2014DN05937A IN 2014DN05937 A IN2014DN05937 A IN 2014DN05937A IN 5937DEN2014 A IN5937DEN2014 A IN 5937DEN2014A IN 2014DN05937 A IN2014DN05937 A IN 2014DN05937A
Authority
IN
India
Prior art keywords
complex
cascade
rna molecule
crispr
activity
Prior art date
Application number
Inventor
Stan Johan Jozef Brouns
Oost John Van Der
Original Assignee
Univ Wageningen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Univ Wageningen filed Critical Univ Wageningen
Publication of IN2014DN05937A publication Critical patent/IN2014DN05937A/en

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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
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    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
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    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21004—Type II site-specific deoxyribonuclease (3.1.21.4)

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  • Health & Medical Sciences (AREA)
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  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Mycology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract

A clustered regularly interspaced short palindromic repeat (CRISPR) associated complex for adaptive antiviral defence(Cascade);the Cascade protein complex comprising at least CRISPR associated protein subunits Cas7 Cas5 and Cas6 which includes at least one subunit with an additional amino acid sequence possessing nucleic acid or chromatin modifying visualising transcription activating or transcription repressing activity. The Cascade complex with additional activity is combined with an RNA molecule to produce a ribonucleoprotein complex. The RNA molecule is selected to have substantial complementarity to a target sequence. Targeted ribonucleoproteins can be used as genetic engineering tools for precise cutting of nucleic acids in homologous recombination non homologous end joining gene modification gene integration mutation repair or for their visualisation transcriptional activation or repression.A pair of ribonucleotides fused to FokI dimers may be used to generate double strand breakages in the DNA to facilitate these applications in a sequence specific manner.
IN5937DEN2014 2011-12-30 2012-12-21 IN2014DN05937A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201122458A GB201122458D0 (en) 2011-12-30 2011-12-30 Modified cascade ribonucleoproteins and uses thereof
PCT/EP2012/076674 WO2013098244A1 (en) 2011-12-30 2012-12-21 Modified cascade ribonucleoproteins and uses thereof

Publications (1)

Publication Number Publication Date
IN2014DN05937A true IN2014DN05937A (en) 2015-06-12

Family

ID=45695084

Family Applications (1)

Application Number Title Priority Date Filing Date
IN5937DEN2014 IN2014DN05937A (en) 2011-12-30 2012-12-21

Country Status (27)

Country Link
US (11) US20140294773A1 (en)
EP (2) EP2798060A1 (en)
JP (1) JP6408914B2 (en)
KR (1) KR101889589B1 (en)
CN (2) CN104321429A (en)
AU (1) AU2012360975B2 (en)
BR (1) BR112014016228B1 (en)
CA (1) CA2862018C (en)
CY (1) CY1120538T1 (en)
DE (1) DE212012000234U1 (en)
DK (1) DK3091072T3 (en)
ES (1) ES2689256T3 (en)
GB (3) GB201122458D0 (en)
HR (1) HRP20181150T1 (en)
HU (1) HUE039617T2 (en)
IL (1) IL233399A0 (en)
IN (1) IN2014DN05937A (en)
LT (1) LT3091072T (en)
MX (1) MX364830B (en)
PL (1) PL3091072T3 (en)
PT (1) PT3091072T (en)
RS (1) RS57604B1 (en)
RU (1) RU2014127702A (en)
SG (1) SG11201403713QA (en)
SI (1) SI3091072T1 (en)
SM (1) SMT201800498T1 (en)
WO (1) WO2013098244A1 (en)

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