AR062336A1 - Recombinacion de homologos mediada por nucleasas en dedo de zinc - Google Patents

Recombinacion de homologos mediada por nucleasas en dedo de zinc

Info

Publication number
AR062336A1
AR062336A1 ARP070103561A ARP070103561A AR062336A1 AR 062336 A1 AR062336 A1 AR 062336A1 AR P070103561 A ARP070103561 A AR P070103561A AR P070103561 A ARP070103561 A AR P070103561A AR 062336 A1 AR062336 A1 AR 062336A1
Authority
AR
Argentina
Prior art keywords
sequence
sequences
vector
chromosomal dna
cell
Prior art date
Application number
ARP070103561A
Other languages
English (en)
Inventor
Lisa Lynn Schulenberg
Qihua C Cai
Jeffrey Miller
William Michael Ainley
Robbi Janette Garrison
Beth C Rubin-Wilson
Joseph F Petolino
Original Assignee
Dow Agrosciences Llc
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Sangamo Biosciences Inc filed Critical Dow Agrosciences Llc
Publication of AR062336A1 publication Critical patent/AR062336A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Abstract

Métodos y composiciones para la integracion dirigida de una secuencia exogena en un sitio blanco predeterminado del genoma de plantas. Reivindicacion 1: Un vector donante que comprende una primera y segunda secuencia de ADN; en donde la primera secuencia es homologa de una tercera secuencia y la segunda secuencia es homologa de una cuarta secuencia; en donde la tercera y cuarta secuencias son secuencias de ADN cromosomico; y en donde los bordes cercanos de tercera y cuarta secuencias están separados por al menos 1 par de nucleotidos. Reivindicacion 2: El vector de la reivindicacion 1, en donde la tercera y cuarta secuencias son secuencias endogenas. Reivindicacion 3: El vector de la reivindicacion 1 o reivindicacion 2, en donde al menos una entre la primera o segunda secuencias tiene una longitud de 100 nucleotidos. Reivindicacion19: Un método para expresar una proteína en una célula vegetal, donde dicho método comprende los pasos de (a) poner la célula en contacto con un vector direccionador de acuerdo con la reivindicacion 8; y (b) expresar una o mas nucleasas en la célula, en donde dichas una o más nucleasas clivan el ADN cromosomico dentro de los 3 pares de kilobases de ya sea la tercera o cuarta secuencia; de modo tal que el clivaje de ADN cromosomico en el paso (b) estimula la incorporacion del vector direccionador en el genoma por recombinacion de homologos.
ARP070103561A 2006-08-11 2007-08-10 Recombinacion de homologos mediada por nucleasas en dedo de zinc AR062336A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US83714706P 2006-08-11 2006-08-11

Publications (1)

Publication Number Publication Date
AR062336A1 true AR062336A1 (es) 2008-10-29

Family

ID=38624400

Family Applications (3)

Application Number Title Priority Date Filing Date
ARP070103561A AR062336A1 (es) 2006-08-11 2007-08-10 Recombinacion de homologos mediada por nucleasas en dedo de zinc
ARP160102220A AR105435A2 (es) 2006-08-11 2016-07-21 Método de recombinación homóloga intramolecular en el genoma de una célula vegetal
ARP170100300A AR108684A2 (es) 2006-08-11 2017-02-06 Recombinación de homólogos mediada por nucleasas con dedos de zinc

Family Applications After (2)

Application Number Title Priority Date Filing Date
ARP160102220A AR105435A2 (es) 2006-08-11 2016-07-21 Método de recombinación homóloga intramolecular en el genoma de una célula vegetal
ARP170100300A AR108684A2 (es) 2006-08-11 2017-02-06 Recombinación de homólogos mediada por nucleasas con dedos de zinc

Country Status (19)

Country Link
US (2) US9428756B2 (es)
EP (2) EP2049663B1 (es)
JP (4) JP2010500029A (es)
KR (1) KR101439568B1 (es)
CN (2) CN101528924B (es)
AR (3) AR062336A1 (es)
AU (1) AU2007284748B2 (es)
BR (1) BRPI0716427A2 (es)
CA (1) CA2660485C (es)
DK (1) DK2049663T3 (es)
ES (1) ES2532002T3 (es)
HK (2) HK1221251A1 (es)
IL (1) IL196942A (es)
PL (1) PL2049663T3 (es)
SI (1) SI2049663T1 (es)
TW (1) TWI424061B (es)
UA (1) UA106193C2 (es)
WO (1) WO2008021207A2 (es)
ZA (1) ZA200900787B (es)

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