AR105435A2 - Método de recombinación homóloga intramolecular en el genoma de una célula vegetal - Google Patents

Método de recombinación homóloga intramolecular en el genoma de una célula vegetal

Info

Publication number
AR105435A2
AR105435A2 ARP160102220A ARP160102220A AR105435A2 AR 105435 A2 AR105435 A2 AR 105435A2 AR P160102220 A ARP160102220 A AR P160102220A AR P160102220 A ARP160102220 A AR P160102220A AR 105435 A2 AR105435 A2 AR 105435A2
Authority
AR
Argentina
Prior art keywords
sequence
genome
intramolecular
recombination method
dna
Prior art date
Application number
ARP160102220A
Other languages
English (en)
Inventor
Lynn Schulenberg Lisa
C Rubin-Wilson Beth
F Petolino Joseph
Janette Garrison Robbi
Michael Ainley William
Miller Jeffrey
C Cai Qihua
Original Assignee
Dow Agrosciences Llc
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Sangamo Biosciences Inc filed Critical Dow Agrosciences Llc
Publication of AR105435A2 publication Critical patent/AR105435A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Un método de recombinación homóloga intramolecular en el genoma de una célula vegetal, excluida la célula que interviene en dicho proceso, donde dicho método comprende el paso de poner en contacto: (a) un segmento de ADN dentro del genoma de la célula vegetal, donde el segmento de ADN comprende una primera secuencia, una segunda secuencia homóloga a la primera secuencia, y una tercer secuencia interpuesta entre la primera y segunda secuencia; (b) con una nucleasa de dedos de zinc, donde la nucleasa cliva el segmento de ADN dentro de la tercera secuencia; y donde el ADN entre la primera y segunda secuencia es eliminado del cromosoma.
ARP160102220A 2006-08-11 2016-07-21 Método de recombinación homóloga intramolecular en el genoma de una célula vegetal AR105435A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US83714706P 2006-08-11 2006-08-11

Publications (1)

Publication Number Publication Date
AR105435A2 true AR105435A2 (es) 2017-10-04

Family

ID=38624400

Family Applications (3)

Application Number Title Priority Date Filing Date
ARP070103561A AR062336A1 (es) 2006-08-11 2007-08-10 Recombinacion de homologos mediada por nucleasas en dedo de zinc
ARP160102220A AR105435A2 (es) 2006-08-11 2016-07-21 Método de recombinación homóloga intramolecular en el genoma de una célula vegetal
ARP170100300A AR108684A2 (es) 2006-08-11 2017-02-06 Recombinación de homólogos mediada por nucleasas con dedos de zinc

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP070103561A AR062336A1 (es) 2006-08-11 2007-08-10 Recombinacion de homologos mediada por nucleasas en dedo de zinc

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP170100300A AR108684A2 (es) 2006-08-11 2017-02-06 Recombinación de homólogos mediada por nucleasas con dedos de zinc

Country Status (19)

Country Link
US (2) US9428756B2 (es)
EP (2) EP2049663B1 (es)
JP (4) JP2010500029A (es)
KR (1) KR101439568B1 (es)
CN (2) CN101528924B (es)
AR (3) AR062336A1 (es)
AU (1) AU2007284748B2 (es)
BR (1) BRPI0716427A2 (es)
CA (1) CA2660485C (es)
DK (1) DK2049663T3 (es)
ES (1) ES2532002T3 (es)
HK (2) HK1221251A1 (es)
IL (1) IL196942A (es)
PL (1) PL2049663T3 (es)
SI (1) SI2049663T1 (es)
TW (1) TWI424061B (es)
UA (1) UA106193C2 (es)
WO (1) WO2008021207A2 (es)
ZA (1) ZA200900787B (es)

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