MX2014009306A - Metodo sin alimentadoras para cultivo de celulas madre espermatogonias de bovino y porcino. - Google Patents

Metodo sin alimentadoras para cultivo de celulas madre espermatogonias de bovino y porcino.

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Publication number
MX2014009306A
MX2014009306A MX2014009306A MX2014009306A MX2014009306A MX 2014009306 A MX2014009306 A MX 2014009306A MX 2014009306 A MX2014009306 A MX 2014009306A MX 2014009306 A MX2014009306 A MX 2014009306A MX 2014009306 A MX2014009306 A MX 2014009306A
Authority
MX
Mexico
Prior art keywords
feeder
stem cells
culture
bovine
spermatogonial stem
Prior art date
Application number
MX2014009306A
Other languages
English (en)
Other versions
MX357280B (es
Inventor
Jon Michael Oatley
Melissa Joan Oatley
Original Assignee
Univ Washington State Res Fdn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Washington State Res Fdn filed Critical Univ Washington State Res Fdn
Publication of MX2014009306A publication Critical patent/MX2014009306A/es
Publication of MX357280B publication Critical patent/MX357280B/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/42—Gynaecological or obstetrical instruments or methods
    • A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
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Abstract

La presente invención se relaciona a la producción y cultivo de células madre espermatogonias no diferenciadas que se pueden mantener a largo plazo y están sin alimentadoras. Las poblaciones sin alimentadoras resultantes se pueden utilizar en cualquiera de un número de protocolos que incluyen la generación de toros de progenie. La presente invención incluye métodos novedosos requeridos para el enriquecimiento exitoso de células madre espermatogonias de bovino, líneas de células novedosas y otros componentes utilizados para las mismas, así como las composiciones de células madre resultantes.
MX2014009306A 2012-02-14 2013-02-11 Método sin alimentadoras para cultivo de células madre espermatogonias de bovino y porcino. MX357280B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261598437P 2012-02-14 2012-02-14
PCT/US2013/025537 WO2013122864A1 (en) 2012-02-14 2013-02-11 Feeder-free method for culture of bovine and porcine spermatogonial stem cells

Publications (2)

Publication Number Publication Date
MX2014009306A true MX2014009306A (es) 2014-10-30
MX357280B MX357280B (es) 2018-07-03

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MX2014009306A MX357280B (es) 2012-02-14 2013-02-11 Método sin alimentadoras para cultivo de células madre espermatogonias de bovino y porcino.

Country Status (11)

Country Link
US (3) US8998793B2 (es)
EP (2) EP3243903A1 (es)
JP (1) JP5782200B2 (es)
CN (2) CN104350145B (es)
AU (1) AU2013221839B2 (es)
BR (1) BR112014020183A8 (es)
CA (1) CA2864510C (es)
EA (1) EA034065B1 (es)
MX (1) MX357280B (es)
NZ (1) NZ627835A (es)
WO (1) WO2013122864A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX357280B (es) 2012-02-14 2018-07-03 Univ Washington State Método sin alimentadoras para cultivo de células madre espermatogonias de bovino y porcino.
US9670458B2 (en) * 2013-07-13 2017-06-06 Aggenetics, Inc. Methods for spermatogonial stem cell (SSC) transfer
KR101777160B1 (ko) * 2014-03-31 2017-09-11 강원대학교산학협력단 포유동물 유래 정원줄기세포의 분리방법
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