MX2013008062A - Seleccion de microcolonias encapsuladas en gel. - Google Patents

Seleccion de microcolonias encapsuladas en gel.

Info

Publication number
MX2013008062A
MX2013008062A MX2013008062A MX2013008062A MX2013008062A MX 2013008062 A MX2013008062 A MX 2013008062A MX 2013008062 A MX2013008062 A MX 2013008062A MX 2013008062 A MX2013008062 A MX 2013008062A MX 2013008062 A MX2013008062 A MX 2013008062A
Authority
MX
Mexico
Prior art keywords
screening
gel
cell
microcolony
encapsulated
Prior art date
Application number
MX2013008062A
Other languages
English (en)
Inventor
Jeremy Agresti
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of MX2013008062A publication Critical patent/MX2013008062A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells

Abstract

Se proveen en la presente métodos y composiciones útiles para detectar la producción de compuestos industrialmente útiles (por ejemplo, isoprenoides, policétidos, y ácidos grasos) en una célula, por ejemplo, una célula microbiana modificada genéticamente para producir uno o más de tales compuestos. En algunas modalidades, los métodos comprenden encapsular la célula en una partícula de hidrogel, y detectar el compuesto dentro de la partícula de hidrogel.
MX2013008062A 2011-01-28 2012-01-27 Seleccion de microcolonias encapsuladas en gel. MX2013008062A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161437214P 2011-01-28 2011-01-28
US201161486211P 2011-05-13 2011-05-13
PCT/US2012/023024 WO2012103516A1 (en) 2011-01-28 2012-01-27 Gel-encapsulated microcolony screening

Publications (1)

Publication Number Publication Date
MX2013008062A true MX2013008062A (es) 2013-08-09

Family

ID=45722697

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013008062A MX2013008062A (es) 2011-01-28 2012-01-27 Seleccion de microcolonias encapsuladas en gel.

Country Status (14)

Country Link
US (1) US20120196770A1 (es)
EP (1) EP2668284B1 (es)
JP (1) JP2014508516A (es)
KR (1) KR20140012072A (es)
CN (1) CN103459610A (es)
AU (1) AU2012211052A1 (es)
BR (1) BR112013017884A2 (es)
CA (1) CA2824420A1 (es)
DK (1) DK2668284T3 (es)
HK (1) HK1186498A1 (es)
MX (1) MX2013008062A (es)
SG (1) SG192107A1 (es)
WO (1) WO2012103516A1 (es)
ZA (1) ZA201304895B (es)

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Also Published As

Publication number Publication date
EP2668284B1 (en) 2014-10-15
WO2012103516A1 (en) 2012-08-02
DK2668284T3 (en) 2014-12-15
JP2014508516A (ja) 2014-04-10
CN103459610A (zh) 2013-12-18
SG192107A1 (en) 2013-08-30
KR20140012072A (ko) 2014-01-29
BR112013017884A2 (pt) 2019-09-24
ZA201304895B (en) 2014-09-25
US20120196770A1 (en) 2012-08-02
AU2012211052A1 (en) 2013-09-05
CA2824420A1 (en) 2012-08-02
EP2668284A1 (en) 2013-12-04
HK1186498A1 (en) 2014-03-14

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