MX2013008062A - Seleccion de microcolonias encapsuladas en gel. - Google Patents
Seleccion de microcolonias encapsuladas en gel.Info
- Publication number
- MX2013008062A MX2013008062A MX2013008062A MX2013008062A MX2013008062A MX 2013008062 A MX2013008062 A MX 2013008062A MX 2013008062 A MX2013008062 A MX 2013008062A MX 2013008062 A MX2013008062 A MX 2013008062A MX 2013008062 A MX2013008062 A MX 2013008062A
- Authority
- MX
- Mexico
- Prior art keywords
- screening
- gel
- cell
- microcolony
- encapsulated
- Prior art date
Links
- 238000012216 screening Methods 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 3
- 239000000017 hydrogel Substances 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 1
- 229930195729 fatty acid Natural products 0.000 abstract 1
- 239000000194 fatty acid Substances 0.000 abstract 1
- 150000004665 fatty acids Chemical class 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 229930001119 polyketide Natural products 0.000 abstract 1
- 125000000830 polyketide group Chemical group 0.000 abstract 1
- 150000003505 terpenes Chemical class 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
Abstract
Se proveen en la presente métodos y composiciones útiles para detectar la producción de compuestos industrialmente útiles (por ejemplo, isoprenoides, policétidos, y ácidos grasos) en una célula, por ejemplo, una célula microbiana modificada genéticamente para producir uno o más de tales compuestos. En algunas modalidades, los métodos comprenden encapsular la célula en una partícula de hidrogel, y detectar el compuesto dentro de la partícula de hidrogel.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161437214P | 2011-01-28 | 2011-01-28 | |
US201161486211P | 2011-05-13 | 2011-05-13 | |
PCT/US2012/023024 WO2012103516A1 (en) | 2011-01-28 | 2012-01-27 | Gel-encapsulated microcolony screening |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2013008062A true MX2013008062A (es) | 2013-08-09 |
Family
ID=45722697
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2013008062A MX2013008062A (es) | 2011-01-28 | 2012-01-27 | Seleccion de microcolonias encapsuladas en gel. |
Country Status (14)
Country | Link |
---|---|
US (1) | US20120196770A1 (es) |
EP (1) | EP2668284B1 (es) |
JP (1) | JP2014508516A (es) |
KR (1) | KR20140012072A (es) |
CN (1) | CN103459610A (es) |
AU (1) | AU2012211052A1 (es) |
BR (1) | BR112013017884A2 (es) |
CA (1) | CA2824420A1 (es) |
DK (1) | DK2668284T3 (es) |
HK (1) | HK1186498A1 (es) |
MX (1) | MX2013008062A (es) |
SG (1) | SG192107A1 (es) |
WO (1) | WO2012103516A1 (es) |
ZA (1) | ZA201304895B (es) |
Families Citing this family (19)
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US20150177115A1 (en) | 2012-04-06 | 2015-06-25 | Slingshot Biosciences | Hydrogel particles with tunable optical properties |
KR101663556B1 (ko) * | 2014-06-13 | 2016-10-07 | 한국생명공학연구원 | 세포를 함유하는 오일 내 하이드로겔 에멀젼, 세포외 구획화 방법 및 신규효소 초고속 스크리닝법 |
EP3214166A4 (en) * | 2014-10-31 | 2018-07-04 | National University Corporation Tokyo University of Agriculture and Technology | Cell isolation method and cell trapping filter |
DE102015106870B3 (de) | 2014-11-10 | 2016-02-25 | Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut | System zur Inkubation von mikrofluidischen Tropfen und Verfahren zur Bereitstellung von homogenen Inkubationsbedingungen in einer Tropfeninkubationseinheit |
KR20230141834A (ko) * | 2015-02-09 | 2023-10-10 | 슬링샷 바이오사이언시즈 인코포레이티드 | 튜닝가능한 광 특성을 갖는 하이드로겔 입자 및 이를 사용하기 위한 방법 |
US11046828B2 (en) * | 2015-04-21 | 2021-06-29 | Trustees Of Tufts College | Fabrication of macroporous monodisperse hydrogel microspheres |
CN107980096B (zh) * | 2015-05-19 | 2020-07-24 | 博瑞创新公司 | 低密度不混溶化合物在用于检测样本中分析物的装置中的用途 |
EP3432854B1 (en) * | 2016-03-24 | 2021-02-17 | Bio-Rad Laboratories, Inc. | Use of gel beads to control droplet dispersion |
CN105907601B (zh) * | 2016-05-12 | 2018-08-21 | 大连理工大学 | 一种基于固态双凝胶的微生物三维群落结构分析芯片及应用 |
WO2018017845A1 (en) * | 2016-07-21 | 2018-01-25 | Massachusetts Intitute Of Technology | Materials and devices containing hydrogel-encapsulated cells |
EP3421131A1 (en) * | 2017-06-30 | 2019-01-02 | Blink AG | A sample cartridge for incubating and/or analyzing a dispersion of particles, cells or droplets |
KR102187682B1 (ko) * | 2018-06-08 | 2020-12-07 | 한국과학기술원 | 타입 iii 폴리케타이드 합성 효소 기반 신규 말로닐-코에이 바이오센서 및 그 용도 |
WO2020076872A1 (en) | 2018-10-08 | 2020-04-16 | Bioelectronica Corporation | Systems and methods for optically processing samples |
TW202141019A (zh) | 2020-01-24 | 2021-11-01 | 美商史林夏特生化公司 | 類細胞校正粒子之組合物與方法 |
CN115485556A (zh) | 2020-05-04 | 2022-12-16 | 弹弓生物科学公司 | 用于多路复用测定的被动光学条形码化的组合物和方法 |
CN111650898B (zh) * | 2020-05-13 | 2023-10-20 | 大唐七台河发电有限责任公司 | 具备高容错性能的集散控制系统及方法 |
CA3212758A1 (en) * | 2021-04-05 | 2022-10-13 | John H. Evans Iv | Yeast strain development for ethanol production |
CN112941007B (zh) * | 2021-04-19 | 2022-06-17 | 广西大学 | 一种香蕉枯萎病菌的单孢分离方法 |
KR20230106910A (ko) * | 2022-01-07 | 2023-07-14 | 인하대학교 산학협력단 | 비색형 바이오센서, 이의 제조방법 및 이를 이용한 항생제 감수성 검사방법 |
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-
2012
- 2012-01-27 US US13/360,620 patent/US20120196770A1/en not_active Abandoned
- 2012-01-27 BR BR112013017884A patent/BR112013017884A2/pt not_active IP Right Cessation
- 2012-01-27 AU AU2012211052A patent/AU2012211052A1/en not_active Abandoned
- 2012-01-27 MX MX2013008062A patent/MX2013008062A/es active IP Right Grant
- 2012-01-27 SG SG2013056239A patent/SG192107A1/en unknown
- 2012-01-27 KR KR1020137022572A patent/KR20140012072A/ko not_active Application Discontinuation
- 2012-01-27 WO PCT/US2012/023024 patent/WO2012103516A1/en active Application Filing
- 2012-01-27 CA CA2824420A patent/CA2824420A1/en not_active Abandoned
- 2012-01-27 DK DK12705186.0T patent/DK2668284T3/en active
- 2012-01-27 EP EP12705186.0A patent/EP2668284B1/en not_active Not-in-force
- 2012-01-27 JP JP2013551394A patent/JP2014508516A/ja active Pending
- 2012-01-27 CN CN2012800150525A patent/CN103459610A/zh active Pending
-
2013
- 2013-07-01 ZA ZA2013/04895A patent/ZA201304895B/en unknown
- 2013-12-10 HK HK13113697.4A patent/HK1186498A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP2668284B1 (en) | 2014-10-15 |
WO2012103516A1 (en) | 2012-08-02 |
DK2668284T3 (en) | 2014-12-15 |
JP2014508516A (ja) | 2014-04-10 |
CN103459610A (zh) | 2013-12-18 |
SG192107A1 (en) | 2013-08-30 |
KR20140012072A (ko) | 2014-01-29 |
BR112013017884A2 (pt) | 2019-09-24 |
ZA201304895B (en) | 2014-09-25 |
US20120196770A1 (en) | 2012-08-02 |
AU2012211052A1 (en) | 2013-09-05 |
CA2824420A1 (en) | 2012-08-02 |
EP2668284A1 (en) | 2013-12-04 |
HK1186498A1 (en) | 2014-03-14 |
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