EA201500142A1 - ШТАММ, ДЕФИЦИТНЫЙ ПО AgsE - Google Patents

ШТАММ, ДЕФИЦИТНЫЙ ПО AgsE

Info

Publication number
EA201500142A1
EA201500142A1 EA201500142A EA201500142A EA201500142A1 EA 201500142 A1 EA201500142 A1 EA 201500142A1 EA 201500142 A EA201500142 A EA 201500142A EA 201500142 A EA201500142 A EA 201500142A EA 201500142 A1 EA201500142 A1 EA 201500142A1
Authority
EA
Eurasian Patent Office
Prior art keywords
host cell
agse
microbial host
modified
production
Prior art date
Application number
EA201500142A
Other languages
English (en)
Inventor
Ноэль Николас Мария Элизабет Пейдж Ван
Мартина Бейсхёйзен
Петер Йозеф Ида Вондерворт Ван Де
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201500142A1 publication Critical patent/EA201500142A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03004—Glucose oxidase (1.1.3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01183—Alpha-1,3-glucan synthase (2.4.1.183)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01004—Phospholipase A2 (3.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)

Abstract

Изобретение относится к мутантной микробной клетке-хозяину, которая является дефицитной по выработке белка AgsE или выработке гомологичного ему белка по сравнению с родительской микробной клеткой-хозяином, которая не была модифицирована, при измерении в одинаковых условиях. Авторами неожиданно было обнаружено, что при использовании мутантной микробной клетки-хозяина по настоящему изобретению в способе для выработки представляющего интерес соединения, например фермента, получают улучшенный выход указанного соединения по сравнению со способом, в котором используется родительская клетка-хозяин, которая не была модифицирована, при измерении в одинаковых условиях.
EA201500142A 2012-07-19 2013-07-19 ШТАММ, ДЕФИЦИТНЫЙ ПО AgsE EA201500142A1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261673596P 2012-07-19 2012-07-19
EP12177172 2012-07-19
PCT/EP2013/065348 WO2014013074A1 (en) 2012-07-19 2013-07-19 Agse-deficient strain

Publications (1)

Publication Number Publication Date
EA201500142A1 true EA201500142A1 (ru) 2015-07-30

Family

ID=49948320

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201500142A EA201500142A1 (ru) 2012-07-19 2013-07-19 ШТАММ, ДЕФИЦИТНЫЙ ПО AgsE

Country Status (13)

Country Link
US (2) US10035986B2 (ru)
EP (2) EP2875119B1 (ru)
JP (1) JP6468561B2 (ru)
CN (1) CN105308171B (ru)
AU (1) AU2013291936B2 (ru)
CA (1) CA2876287C (ru)
CL (1) CL2015000084A1 (ru)
DK (2) DK2875119T3 (ru)
EA (1) EA201500142A1 (ru)
IN (1) IN2014DN11163A (ru)
MX (1) MX357482B (ru)
NZ (1) NZ702962A (ru)
WO (1) WO2014013074A1 (ru)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6132847B2 (ja) 2012-11-09 2017-05-24 国立大学法人東北大学 糸状菌の高密度培養株を用いた有用物質生産方法
MX370305B (es) 2013-12-02 2019-12-09 Dsm Ip Assets Bv Proteína estructurante de hielo.
WO2016026839A1 (en) 2014-08-21 2016-02-25 Dsm Ip Assets B.V. Use of penicillium glucose oxidase in the baking industry for replacing bromate or azodicarbonamide
US20170313997A1 (en) * 2014-10-29 2017-11-02 Novozymes A/S Filamentous Fungal Double-Mutant Host Cells
WO2016110453A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a filamentous fungal host cell
EP3280264B1 (en) 2015-04-10 2021-01-13 DSM IP Assets B.V. Method for preparing a dough
US10844397B2 (en) 2015-06-01 2020-11-24 Arizona Board Of Regents On Behalf Of The University Of Arizona Transgenic plant species engineered to inhibit biosynthesis of Aspergillus aflatoxin
NZ737305A (en) 2015-06-02 2021-07-30 Dsm Ip Assets Bv Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
CN105950482B (zh) * 2016-07-15 2019-04-02 南京林业大学 一株产菊粉酶的菌株及其应用
US10913938B2 (en) 2016-07-29 2021-02-09 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114941A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
BR112019012559A2 (pt) 2016-12-21 2019-11-26 Dsm Ip Assets Bv variantes de enzima lipolítica
EP3596199A1 (en) * 2017-03-13 2020-01-22 DSM IP Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
EP3809852A1 (en) 2018-06-19 2021-04-28 DSM IP Assets B.V. Lipolytic enzyme variants
CN110184195B (zh) * 2019-04-25 2020-11-17 广东海洋大学 一株高产油脂的桔青霉Asc2-4-1及其应用
CN110713937B (zh) * 2019-09-20 2022-02-01 兰州大学 提高中华羊茅-内生真菌菌株pa次生代谢产物波胺含量的方法
CN111849809B (zh) * 2020-07-09 2021-12-24 南京农业大学 一种可降解菊粉果聚糖的菌株及其应用
CN112662572B (zh) * 2021-02-02 2022-03-15 中国科学院海洋研究所 一种高产壳聚糖酶的菌株及其应用
EP4359426A2 (en) 2021-06-24 2024-05-01 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198345A (en) 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
CA1341226C (en) 1988-08-16 2001-05-01 Wim Van Hartingsveldt Gene replacement as a tool for the construction of aspergillus strains
FR2679920A1 (fr) 1991-08-02 1993-02-05 Rhone Poulenc Rorer Sa Levures recombinantes hautement stables pour la production de proteines recombinantes, leur preparation et leur utilisation.
ES2198410T3 (es) 1993-07-23 2004-02-01 Dsm Ip Assets B.V. Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion.
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5602004A (en) * 1994-07-20 1997-02-11 Novo Nordisk Biotech, Inc. Thermophilic fungal expression system
CA2201730C (en) 1995-08-03 2008-01-22 Bart Willem Swinkels The use of homologous amds genes as selectable markers
WO1998046772A2 (en) 1997-04-11 1998-10-22 Dsm N.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
CN1198924C (zh) 1997-12-22 2005-04-27 Dsm公司 在丝状真菌中表达克隆
JP4563585B2 (ja) 1998-10-05 2010-10-13 ノボザイムス アクティーゼルスカブ ポリペプチドの生成方法において有用な菌類転写活性化因子
DE60025467T2 (de) 1999-02-22 2006-09-21 Novozymes A/S Oxalacetat-hydrolase defiziente fungale wirtszellen
CN100532561C (zh) 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
WO2001021779A2 (en) 1999-09-17 2001-03-29 Dsm N.V. Penicillium chrysogenum transcriptional activator blar, a pathway specific regulator of beta-lactam biosynthesis, and uses therof
WO2001068864A1 (en) 2000-03-14 2001-09-20 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
DE60133884D1 (de) 2000-03-24 2008-06-19 Genencor Int Herstellung von sekretierten proteinen durch rekombinante eukaryotische zellen
US7229784B2 (en) * 2000-09-19 2007-06-12 Microbia, Inc. Modulation of secondary metabolite production by zinc binuclear cluster proteins
US20060088902A1 (en) * 2001-08-28 2006-04-27 Van Den Hondel Cornelis A Methods and materials for the identification of antifungal substrates in filamentous fungi
CA2513833A1 (en) 2003-02-05 2004-08-19 Dsm Ip Assets B.V. Use of oxalate deficient aspergillus niger strains for producing a polypeptide
ES2294958B1 (es) 2003-07-15 2009-02-16 Mintek Procedimiento de lixiviacion oxidativa.
WO2005026356A1 (en) 2003-09-12 2005-03-24 Commonwealth Scientific And Industrial Research Organisation Modified gene-silencing nucleic acid molecules and uses thereof
EP2172557B1 (en) 2004-04-02 2018-03-14 DSM IP Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
EP2345726A3 (en) 2004-04-16 2011-12-14 DSM IP Assets B.V. Fungal promoter for expressing a gene in a fungal cell
ES2274664B1 (es) * 2004-04-30 2008-04-01 Consejo Sup. Investig. Cientificas Levaduras bgs4delta autoliticas, procedimiento de obtencion y sus aplicaciones en procedimientos de expresion de proteinas.
CA2568788A1 (en) 2004-06-16 2005-12-29 Dsm Ip Assets B.V. Production of polypeptides by improved secretion
US7794974B2 (en) 2004-10-12 2010-09-14 Dsm Ip Assets B.V. Fungal transcriptional activators useful in methods for producing a polypeptide
EP1799829B1 (en) 2004-10-15 2012-01-18 DSM IP Assets B.V. Method for production of a compound in a eukaryotic cell
EP1799821A2 (en) 2004-10-15 2007-06-27 DSMIP Assets B.V. Homologous amds genes as selectable marker
PL2410048T3 (pl) 2005-01-24 2017-02-28 Dsm Ip Assets B.V. Sposób otrzymywania związku będącego przedmiotem zainteresowania w komórce grzyba nitkowatego
EP1856263A1 (en) 2005-03-01 2007-11-21 DSMIP Assets B.V. Aspergillus promotors for expressing a gene in a fungal cell
CA2630455A1 (en) 2005-11-29 2007-06-07 Dsm Ip Assets B.V. Dna binding site of a transcriptional activator useful in gene expression
BRPI0713795B1 (pt) 2006-06-29 2018-03-20 Dsm Ip Assets B.V. Método de otimização de uma sequência de nucleotídeos codificadora que codifica uma sequência de aminoácidos predeterminada
WO2008053019A2 (en) 2006-11-02 2008-05-08 Dsm Ip Assets B.V. Method for reducing the expression of a gene in a filamentous fungal cell
WO2008098933A1 (en) 2007-02-15 2008-08-21 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
DK2254996T3 (da) 2008-02-29 2013-10-07 Dsm Ip Assets Bv Lipaser med høj specificitet for kortkædede fedtsyrer og anvendelser deraf
ES2334421B1 (es) * 2008-05-29 2011-02-28 Consejo Superior De Investigaciones Cientificas (Csic) Levaduras vinicas recombinantes.
EA021205B1 (ru) 2009-03-10 2015-04-30 ДСМ АйПи АССЕТС Б.В. Способ повышения выхода полипептидов
US20120041171A1 (en) 2009-04-22 2012-02-16 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
EP3293264A1 (en) 2009-07-22 2018-03-14 DSM IP Assets B.V. Improved host cell for the production of a compound of interest
EA201300074A1 (ru) 2010-07-01 2013-06-28 ДСМ АйПи АССЕТС Б.В. Способ получения представляющего интерес соединения
CN102618606B (zh) * 2011-01-30 2014-09-10 浙江海正药业股份有限公司 一种棘白菌素生物转化方法

Also Published As

Publication number Publication date
MX357482B (es) 2018-07-11
CA2876287A1 (en) 2014-01-23
US10035986B2 (en) 2018-07-31
CN105308171B (zh) 2019-03-08
US20150191705A1 (en) 2015-07-09
US20170175158A1 (en) 2017-06-22
DK2875119T3 (en) 2018-04-03
JP6468561B2 (ja) 2019-02-13
EP3327112B1 (en) 2020-02-12
IN2014DN11163A (ru) 2015-10-02
MX2015000840A (es) 2015-04-08
JP2015522288A (ja) 2015-08-06
WO2014013074A1 (en) 2014-01-23
EP3327112A1 (en) 2018-05-30
CN105308171A (zh) 2016-02-03
US10100344B2 (en) 2018-10-16
EP2875119A1 (en) 2015-05-27
CL2015000084A1 (es) 2015-05-04
AU2013291936B2 (en) 2019-01-17
EP2875119B1 (en) 2017-12-20
DK3327112T3 (en) 2020-05-04
CA2876287C (en) 2022-11-22
NZ702962A (en) 2018-02-23
AU2013291936A1 (en) 2015-01-22

Similar Documents

Publication Publication Date Title
EA201500142A1 (ru) ШТАММ, ДЕФИЦИТНЫЙ ПО AgsE
MX2015000844A (es) Cepa con deficit de amilasa.
EA201300074A1 (ru) Способ получения представляющего интерес соединения
WO2014143744A3 (en) Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same
MX367859B (es) Metodos y materiales para la biosintesis de compuestos mogrosidos.
MX2013008062A (es) Seleccion de microcolonias encapsuladas en gel.
MX2019004568A (es) Produccion mejorada de derivados de acidos grasos.
WO2013185184A3 (en) Production of long chain polyunsaturated fatty acids in plant cells
WO2012173659A3 (en) Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation
PL2890793T3 (pl) Wytwarzanie lotnych dienów przez dehydratację enzymatyczną (EC 4.2.1.127) lekkich alkenoli
WO2015004403A3 (fr) Procede de culture cellulaire decouple
MY182946A (en) Production of organic acids by fermentation at low ph
MX2020001357A (es) Produccion microbiana de aminas grasas.
WO2013181575A3 (en) Methods related to denosumab
FI20115328A0 (fi) Uusia kutinaaseja, niiden tuottaminen ja käytöt
EP4282972A3 (en) Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
NZ720340A (en) Synthesis of trans-8-chloro-5-methyl-1 -[4-(pyridin-2-yloxy)-cyclohexyl]-5,6-dihydro-4h-2,3,5,10b-tetraaza-benzo[e]azulene and crystalline forms thereof
WO2014182684A3 (en) Methods and compositions related to large scale production of proteins
UY34386A (es) Método para la producción de ácido láctico
TR201904928T4 (tr) Tereftalik asit diesterlerin resirküle alkol dehidratasyonu ile üretimi için yöntem.
WO2013181599A3 (en) Methods related to rituximab
EA201301294A1 (ru) Получение высокочистых мультисубъединичных белков, таких как антитела, в трансформированных микроорганизмах, например pichia pastoris
WO2013092965A3 (en) Bacteria with reconstructed transcriptional units and the uses thereof
IN2014DN07407A (ru)
EA201692057A1 (ru) Композиции и способы повышения производства