DE60133884D1 - Herstellung von sekretierten proteinen durch rekombinante eukaryotische zellen - Google Patents
Herstellung von sekretierten proteinen durch rekombinante eukaryotische zellenInfo
- Publication number
- DE60133884D1 DE60133884D1 DE60133884T DE60133884T DE60133884D1 DE 60133884 D1 DE60133884 D1 DE 60133884D1 DE 60133884 T DE60133884 T DE 60133884T DE 60133884 T DE60133884 T DE 60133884T DE 60133884 D1 DE60133884 D1 DE 60133884D1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- secreted
- proteins
- secretated
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/11—Protein-serine/threonine kinases (2.7.11)
- C12Y207/11001—Non-specific serine/threonine protein kinase (2.7.11.1), i.e. casein kinase or checkpoint kinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
- C12Y301/03016—Phosphoprotein phosphatase (3.1.3.16), i.e. calcineurin
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US53469200A | 2000-03-24 | 2000-03-24 | |
PCT/US2001/009401 WO2001072783A2 (en) | 2000-03-24 | 2001-03-23 | Production of secreted proteins by recombinant eukaryotic cells |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60133884D1 true DE60133884D1 (de) | 2008-06-19 |
Family
ID=24131126
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60133884T Expired - Lifetime DE60133884D1 (de) | 2000-03-24 | 2001-03-23 | Herstellung von sekretierten proteinen durch rekombinante eukaryotische zellen |
Country Status (8)
Country | Link |
---|---|
US (4) | US20010034045A1 (de) |
EP (1) | EP1266018B1 (de) |
AT (1) | ATE394496T1 (de) |
AU (1) | AU2001250954A1 (de) |
CA (1) | CA2403842C (de) |
DE (1) | DE60133884D1 (de) |
DK (1) | DK1266018T3 (de) |
WO (1) | WO2001072783A2 (de) |
Families Citing this family (57)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9821198D0 (en) | 1998-09-30 | 1998-11-25 | Danisco | Enzyme |
US7312062B2 (en) | 1998-11-27 | 2007-12-25 | Novozymes A/S | Lipolytic enzyme variants |
US9057061B2 (en) | 2003-12-23 | 2015-06-16 | Novozymes Biopharma Dk A/S | Gene expression technique |
DK2339015T3 (en) * | 2004-03-31 | 2018-08-06 | Janssen Biotech Inc | Methods for changing protein production rates |
KR20070053732A (ko) * | 2004-09-02 | 2007-05-25 | 와이어쓰 | 단백질 생산 시스템 및 생산 방법 |
US7888489B2 (en) | 2005-01-24 | 2011-02-15 | Dsm Ip Assets B.V. | Method for producing a compound of interest in a filamentous fungal cell |
GB0512707D0 (en) * | 2005-06-22 | 2005-07-27 | Delta Biotechnology Ltd | Gene expression technique |
US8071089B2 (en) | 2005-11-01 | 2011-12-06 | Bio-Cat, Inc. | Composition with a fungal (yeast) lipase and method for treating lipid malabsorption in cystic fibrosis as well as people suffering from pancreatic lipase insufficiency |
EP1954812B1 (de) | 2005-11-29 | 2012-11-21 | DSM IP Assets B.V. | Dna-bindungsstelle eines transkriptionsaktivators mit eignung bei der genexpression |
CN101605887B (zh) * | 2006-05-16 | 2012-09-05 | 协和发酵麒麟株式会社 | 蛋白的高分泌生产方法 |
KR100977446B1 (ko) * | 2006-06-16 | 2010-08-24 | 한국생명공학연구원 | 분비 스트레스 반응을 조절하는 한세눌라 폴리모르파의신규한 유전자 및 상기 유전자를 이용하여 재조합 단백질의분비 발현 효율을 증가시키는 방법 |
JP5250850B2 (ja) | 2006-06-29 | 2013-07-31 | ディーエスエム アイピー アセッツ ビー.ブイ. | 改善されたポリペプチド発現を達成する方法 |
EP2082046A2 (de) * | 2006-11-02 | 2009-07-29 | DSMIP Assets B.V. | Verbesserte produktion sezernierter proteine durch filamentöse pilze |
US20100062491A1 (en) * | 2007-01-05 | 2010-03-11 | Novozymes A/S | Overexpression of the Chaperone BIP in a Heterokaryon |
CN101784666A (zh) | 2007-02-15 | 2010-07-21 | 帝斯曼知识产权资产管理有限公司 | 用于生产感兴趣的化合物的重组宿主细胞 |
EP2129685B1 (de) * | 2007-03-21 | 2013-11-20 | Danisco US Inc. | Überexpression von foldasen und chaperonen für erhöhte proteinproduktion |
WO2009058956A1 (en) * | 2007-11-01 | 2009-05-07 | Danisco Us, Inc. | Signal sequences and co-expressed chaperones for improving protein production in a host cell |
CN101952303A (zh) * | 2008-02-25 | 2011-01-19 | 诺维信公司 | 提高目标蛋白质的表达产率的方法 |
US20120041171A1 (en) | 2009-04-22 | 2012-02-16 | Dsm Ip Assets B.V. | Process for the production of a recombinant polypeptide of interest |
BRPI1015536A2 (pt) | 2009-04-24 | 2019-07-02 | Dsm Ip Assets Bv | polipeptídeo de degradação de carboidratos e usos do mesmo |
EP2258854A1 (de) | 2009-05-20 | 2010-12-08 | FH Campus Wien | Eukaryotische Wirtszelle mit einem Ausdrucksverstärker |
CA2768608A1 (en) | 2009-07-22 | 2011-01-27 | Dsm Ip Assets B.V. | Improved host cell for the production of a compound of interest |
EP2531590A2 (de) | 2010-02-04 | 2012-12-12 | DSM IP Assets B.V. | Verfahren zur herstellung von filamentösen pilzstämmen mit einem sexuellen zyklus und verfahren zur herstellung sexuell gekreuzter, filamentöser pilzstämme |
AU2011214324B2 (en) | 2010-02-11 | 2014-11-20 | Dsm Ip Assets B.V. | Host cell capable of producing enzymes useful for degradation of lignocellulosic material |
EA201201126A1 (ru) | 2010-02-11 | 2013-05-30 | ДСМ АйПи АССЕТС Б.В. | Полипептид, обладающий активностью целлобиогидролазы, и его применение |
FI20105632A0 (fi) | 2010-06-04 | 2010-06-04 | Valtion Teknillinen | Menetelmä proteiinien tuottamiseksi rihmamaisilla sienillä |
FI20105633A0 (fi) * | 2010-06-04 | 2010-06-04 | Valtion Teknillinen | Parannettu menetelmä proteiinien tuottamiseksi |
FI20105634A0 (fi) * | 2010-06-04 | 2010-06-04 | Valtion Teknillinen | Proteiinien parannettu tuotto rihmamaisilla sienillä |
EP2580232A1 (de) * | 2010-06-08 | 2013-04-17 | National University of Ireland, Galway | Manipulation von hsp70- und ire1alpha-proteininteraktionen |
CA2803986C (en) | 2010-06-29 | 2019-04-23 | Dsm Ip Assets B.V. | Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof |
CA2801824A1 (en) | 2010-06-29 | 2012-01-05 | Dsm Ip Assets B.V. | Polypeptide having swollenin activity and uses thereof |
CN103119156A (zh) | 2010-06-29 | 2013-05-22 | 帝斯曼知识产权资产管理有限公司 | 具有降解碳水化合物活性的多肽及其用途 |
MX2012015144A (es) | 2010-06-29 | 2013-03-05 | Dsm Ip Assets Bv | Polipeptido que tiene actividad de acetil-xilan-esterasa y usos del mismo. |
PL2588494T3 (pl) | 2010-06-29 | 2018-08-31 | Dsm Ip Assets B.V. | Polipeptyd mający aktywność beta-glukozydazy i jego zastosowania |
CN102971338A (zh) | 2010-06-29 | 2013-03-13 | 帝斯曼知识产权资产管理有限公司 | 具有β-葡糖苷酶活性的多肽及其用途 |
US8945898B2 (en) | 2010-07-01 | 2015-02-03 | Dsm Ip Assets B.V. | Recombinant host cell with deficiency in non-ribosomal peptide synthase production |
JPWO2012060389A1 (ja) * | 2010-11-05 | 2014-05-12 | 旭硝子株式会社 | シゾサッカロミセス属酵母の形質転換体およびその製造方法 |
CA2819552C (en) * | 2010-12-09 | 2023-09-26 | Institut Pasteur | Mgmt-based method for obtaining high yield of recombinant protein expression |
WO2012109238A2 (en) | 2011-02-07 | 2012-08-16 | President And Fellows Of Harvard College | Methods for increasing immune responses using agents that directly bind to and activate ire-1 |
WO2013134774A1 (en) | 2012-03-09 | 2013-09-12 | Cornell University | Modulation of breast cancer growth by modulation of xbp1 activity |
CN102604913B (zh) * | 2012-04-05 | 2013-08-14 | 湖南尤特尔生化有限公司 | 一种疏棉状嗜热丝孢菌脂肪酶的制备方法和应用 |
WO2013181647A2 (en) | 2012-06-01 | 2013-12-05 | Danisco Us Inc. | Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms |
EP2875119B1 (de) | 2012-07-19 | 2017-12-20 | DSM IP Assets B.V. | Agse-armer stamm |
CN102994541B (zh) * | 2012-12-19 | 2015-04-15 | 江南大学 | 一种共表达upr关键基因和下游靶基因增强葡萄糖氧化酶分泌的方法 |
WO2014161936A1 (en) * | 2013-04-03 | 2014-10-09 | Novozymes A/S | A filamentous fungal cell with inactivated component of the selective autophagy pathway and method of using same |
ES2724300T3 (es) | 2013-09-25 | 2019-09-10 | Univ Cornell | Compuestos para inducir inmunidad antitumoral y métodos de lo mismo |
WO2015158800A1 (en) | 2014-04-17 | 2015-10-22 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Recombinant host cell for expressing proteins of interest |
CA2948369C (en) | 2014-04-17 | 2023-09-26 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Recombinant host cell engineered to overexpress helper proteins |
CN105112303B (zh) * | 2015-09-06 | 2018-10-16 | 江南大学 | 一种产酒用复合酶的黑曲霉菌株 |
CN109476714A (zh) * | 2016-07-22 | 2019-03-15 | 诺维信公司 | 改善的丝状真菌宿主 |
US20200063166A1 (en) | 2017-03-13 | 2020-02-27 | Dsm Ip Assets B.V. | Zinc binuclear cluster transcriptional regulator-deficient strain |
CN107723253B (zh) * | 2017-09-29 | 2021-10-15 | 天津昕因达生物技术有限公司 | 一种双质粒共转化外源基因高表达基因工程菌 |
WO2019195382A1 (en) * | 2018-04-06 | 2019-10-10 | Danisco Us Inc | Increased alcohol production from yeast producing an increased amount of active hac1 protein |
WO2019213521A1 (en) * | 2018-05-04 | 2019-11-07 | Board Of Trustees Of Michigan State University | Expression of unfolded protein response proteins improves plant biomass and growth |
WO2020112911A1 (en) * | 2018-11-28 | 2020-06-04 | Novozymes A/S | Improved filamentous fungal host cells |
CN110358781B (zh) * | 2019-07-31 | 2022-01-28 | 湖北大学 | 一种酸性哺乳动物几丁质酶编码基因和应用 |
WO2023039358A1 (en) * | 2021-09-09 | 2023-03-16 | Dupont Nutrition Biosciences Aps | Over expression of foldases and chaperones improves protein production |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2353384A (en) | 1983-01-19 | 1984-07-26 | Genentech Inc. | Amplification in eukaryotic host cells |
US5364770A (en) * | 1985-08-29 | 1994-11-15 | Genencor International Inc. | Heterologous polypeptides expressed in aspergillus |
US5010182A (en) * | 1987-07-28 | 1991-04-23 | Chiron Corporation | DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides |
GB8724885D0 (en) | 1987-10-23 | 1987-11-25 | Binns M M | Fowlpox virus promotors |
AU4005289A (en) | 1988-08-25 | 1990-03-01 | Smithkline Beecham Corporation | Recombinant saccharomyces |
FR2646437B1 (fr) | 1989-04-28 | 1991-08-30 | Transgene Sa | Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant |
US5665585A (en) * | 1992-09-03 | 1997-09-09 | Alko-Yhiot Oy | Recombinant production of glucoamylase P in trichoderma |
US5541094A (en) * | 1992-09-25 | 1996-07-30 | E. I. Du Pont De Nemours And Company | Glyoxylic acid/aminomethylphosphonic acid mixtures prepared using a microbial transformant |
US5861271A (en) * | 1993-12-17 | 1999-01-19 | Fowler; Timothy | Cellulase enzymes and systems for their expressions |
JPH1084970A (ja) * | 1996-07-23 | 1998-04-07 | H S P Kenkyusho:Kk | 新規転写調節因子 |
-
2001
- 2001-03-23 CA CA2403842A patent/CA2403842C/en not_active Expired - Fee Related
- 2001-03-23 EP EP01924287A patent/EP1266018B1/de not_active Expired - Lifetime
- 2001-03-23 WO PCT/US2001/009401 patent/WO2001072783A2/en active Application Filing
- 2001-03-23 DK DK01924287T patent/DK1266018T3/da active
- 2001-03-23 DE DE60133884T patent/DE60133884D1/de not_active Expired - Lifetime
- 2001-03-23 US US09/816,277 patent/US20010034045A1/en not_active Abandoned
- 2001-03-23 AU AU2001250954A patent/AU2001250954A1/en not_active Abandoned
- 2001-03-23 AT AT01924287T patent/ATE394496T1/de not_active IP Right Cessation
-
2003
- 2003-09-15 US US10/663,450 patent/US7662584B2/en not_active Expired - Fee Related
-
2009
- 2009-12-16 US US12/639,921 patent/US8753866B2/en not_active Expired - Fee Related
-
2014
- 2014-05-08 US US14/272,629 patent/US20140335591A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CA2403842C (en) | 2012-09-25 |
AU2001250954A1 (en) | 2001-10-08 |
US20100221775A1 (en) | 2010-09-02 |
US8753866B2 (en) | 2014-06-17 |
US20040186070A1 (en) | 2004-09-23 |
WO2001072783A2 (en) | 2001-10-04 |
DK1266018T3 (da) | 2008-09-01 |
EP1266018B1 (de) | 2008-05-07 |
US20140335591A1 (en) | 2014-11-13 |
US7662584B2 (en) | 2010-02-16 |
EP1266018A2 (de) | 2002-12-18 |
CA2403842A1 (en) | 2001-10-04 |
WO2001072783A3 (en) | 2002-07-18 |
US20010034045A1 (en) | 2001-10-25 |
ATE394496T1 (de) | 2008-05-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |